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公开(公告)号：US09150817B2
公开(公告)日：2015-10-06
申请号：US12296786
申请日：2007-04-13
CPC分类号： C11C3/003 , C07K14/195 , C07K14/21 , C11C3/00 , C11C3/04 , C12P7/64 , C12P7/6427 , C12P7/6436 , C12P7/6445 , C12P7/6454 , C12P7/6472
摘要： The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.
摘要翻译： 本发明提供一种浓缩PUFA油的方法，其特征在于使用脂肪酶的醇解反应在少量水和至少一种选自氧化镁，氢氧化镁，氧化钙和钙的添加剂的化合物存在下进行 氢氧化物，然后进行分离以获得甘油酯级分。
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公开(公告)号：US20100190220A1
公开(公告)日：2010-07-29
申请号：US12669803
申请日：2008-07-29
IPC分类号： C12P7/64
CPC分类号： C11C1/045 , C11C3/10 , C12P7/6472
摘要： Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.
摘要翻译： 含有EPA和DHA的油和脂肪的醇解通过对具有18个或更少碳原子的脂肪酸具有底物特异性的脂肪酶和在诸如氧化镁的反应添加剂存在下进行。 然后分离甘油酯级分; 甘油酯级分的醇解通过对具有20个碳以下的脂肪酸和在氧化镁等反应添加剂的存在下具有底物特异性的脂肪酶进行， 并同时获得富含EPA的油和DHA富集油。
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公开(公告)号：US06905850B2
公开(公告)日：2005-06-14
申请号：US10311350
申请日：2001-07-02
申请人： Roxana Irimescu , Kiyomi Furihata , Kazuhiko Hata , Tsuneo Yamane
发明人： Roxana Irimescu , Kiyomi Furihata , Kazuhiko Hata , Tsuneo Yamane
CPC分类号： C12P7/6445 , C12P7/6472
摘要： The invention provides a process for producing 2-monoglycerides (2-MG) or triglycerides (TG), particularly triglycerides having polyunsaturated fatty acid (PUFA) residues in the sn-2 positions, at an extremely high purity and a high efficiency.The 2-MG is produced by conducting alcoholysis of a starting TG with a first 1,3-lipase. Then, the TG is produced by introducing fatty acid residues to the 1- and 3-positions of the produced 2-MG by using a second 1,3-lipase. The fatty acid residue in the 2-posiiton is preferably a residue of DHA, EPA or ARA. The “first lipase” refers to one which is selective for the 1- and 3-positions and can act even on a long-chain fatty acid in alcoholysis, while the “second lipase” refers to one which is selective for the 1- and 3-positions and can produce a TG through the reaction of a 2-MG with a fatty acid ester or a free fatty acid.
摘要翻译： 本发明提供了以非常高的纯度和高效率生产2-单甘油酯（2-MG）或甘油三酸酯（TG），特别是在sn-2位具有多不饱和脂肪酸（PUFA）残基的甘油三酯）的方法。 通过用第一种1,3-脂肪酶进行起始TG的醇解来制备2-MG。 然后，通过使用第二种1,3-脂肪酶将所产生的2-MG的1-和3-位引入脂肪酸残基来产生TG。 2-位的脂肪酸残基优选为DHA，EPA或ARA的残基。 “第一脂肪酶”是指对于1-和3-位选择性的那些，并且甚至可以在醇解中的长链脂肪酸上起作用，而“第二脂肪酶”是指对于1-和 3-位，并且可以通过2-MG与脂肪酸酯或游离脂肪酸的反应产生TG。
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公开(公告)号：US6117905A
公开(公告)日：2000-09-12
申请号：US284205
申请日：1999-06-29
CPC分类号： A23D9/00 , A23L33/12 , A23L33/40 , A23V2002/00
摘要： Edible oil containing arachidonic acid obtained from microorganisms belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid are provided. The oil contain little unsaponifiable matters and, above all, the smallest possible amount of sterol with cyclopropane structure which have not been recognized as food components, and are suitable for the production of foods, in particular, infant formula.Arachidonic acid-containing edible oil originating in microorganisms containing not more than 0.8% by weight, preferably not more than 0.6% by weight of unsaponifiable matters and 20% by weight or more of arachidonic acid. Further, these edible oil contain not more than 0.3% by weight, preferably not more than 0.15% by weight of 24,25-methylenecholest-5-en-3 .beta.-ol. The microorganisms are those belonging to the subgenus Mortierella of the genus Mortierella and being capable of producing arachidonic acid. These microorganisms belong to the species alpine of the genus Mortierella. Foods including the arachidonic acid-containing edible oil. Formula for premature infants, formula for infants, foods for infants, and foods for pregnant women and nursing mothers, including the arachidonic acid-containing edible oil.
摘要翻译： PCT No.PCT / JP97 / 03631 Sec。 371日期1999年6月29日第 102（e）1999年6月29日PCT PCT 1997年10月9日PCT公布。 公开号WO98 / 16119 日期：1998年04月23日提供了从属于被孢霉属的被孢霉属的属于微生物的微生物获得的能够产生花生四烯酸的花生四烯酸的可食用油。 该油含有少量不皂化物质，最重要的是尽可能少量的具有环丙烷结构的固醇未被认定为食品成分，并且适用于生产食品，特别是婴儿配方奶粉。 来源于不超过0.8重量％，优选不超过0.6重量％的不皂化物和20重量％以上花生四烯酸的微生物的含花生四烯酸的食用油。 此外，这些食用油含有不多于0.3重量％，优选不超过0.15重量％的24,25-亚甲基胆甾-5-烯-3β-醇。 这些微生物属于被孢霉属的被孢霉属亚属属，并且能够产生花生四烯酸。 这些微生物属于被孢霉属的高山属。 食物包括含花生四烯酸的食用油。 婴幼儿配方奶粉，婴儿配方食品，孕妇食品，哺乳母亲，包括含花生四烯酸的食用油。
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公开(公告)号：US20090176284A1
公开(公告)日：2009-07-09
申请号：US12296786
申请日：2007-04-13
IPC分类号： C12P7/64
CPC分类号： C11C3/003 , C07K14/195 , C07K14/21 , C11C3/00 , C11C3/04 , C12P7/64 , C12P7/6427 , C12P7/6436 , C12P7/6445 , C12P7/6454 , C12P7/6472
摘要： The present invention provides a process for concentrated PUFA oil, characterized in that alcoholysis reaction using lipase is carried out in the presence of a small amount of water and at least one compound as an additive selected from magnesium oxide, magnesium hydroxide, calcium oxide and calcium hydroxide, and then separation is conducted to obtain a glyceride fraction.
摘要翻译： 本发明提供一种浓缩PUFA油的方法，其特征在于使用脂肪酶的醇解反应在少量水和至少一种选自氧化镁，氢氧化镁，氧化钙和钙的添加剂的化合物存在下进行 氢氧化物，然后进行分离以获得甘油酯级分。
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公开(公告)号：US09556401B2
公开(公告)日：2017-01-31
申请号：US12669803
申请日：2008-07-29
CPC分类号： C11C1/045 , C11C3/10 , C12P7/6472
摘要： Alcoholysis of oils and fats containing EPA and DHA is performed by a lipase having substrate specificity for fatty acids having 18 carbons or less and in the presence of a reaction additive such as magnesium oxide; then the glyceride fraction is separated; alcoholysis of the glyceride fraction is performed by a lipase having substrate specificity for fatty acids having 20 carbons or less and in the presence of a reaction additive such as magnesium oxide; and EPA-enriched oil and DHA-enriched oil are simultaneously obtained.
摘要翻译： 含有EPA和DHA的油和脂肪的醇解通过对具有18个或更少碳原子的脂肪酸具有底物特异性的脂肪酶和在诸如氧化镁的反应添加剂存在下进行。 然后分离甘油酯级分; 甘油酯级分的醇解通过对具有20个碳以下的脂肪酸和在氧化镁等反应添加剂的存在下具有底物特异性的脂肪酶进行， 同时获得富含EPA的油和富含DHA的油。
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公开(公告)号：US5840944A
公开(公告)日：1998-11-24
申请号：US232021
申请日：1994-06-27
申请人： Kiyomi Furihata , Kazuhiko Hata
发明人： Kiyomi Furihata , Kazuhiko Hata
IPC分类号： C07C69/587 , C07C51/44 , C07C57/03 , C07C67/54 , C07C67/56 , C11B3/10 , C11B3/12 , C11C1/08 , C11C1/10
摘要： A mixture of fatty acids or their esters derived from natural oils and fats including eicosapentaenoic acids or their derivatives is precision distilled under a high vacuum using a plurality of distillation columns, three distillation columns or more in particular, in order to acquire a fraction consisting mainly of fatty acids of carbon number 20 or their esters, which is then subjected to a column chromatography with a reversed-phase distribution system. Eicosapentaenoic acids or their esters, which is useful for preventing or treating thrombus, with a purity as high as 99% and above and with a recovery rate 55% or above, is produced.
摘要翻译： PCT No.PCT / JP92 / 01393 Sec。 371日期1994年6月27日第 102（e）日期1994年6月27日PCT提交1992年10月28日PCT公布。 第WO93 / 09210号公报 日期1993年5月13日由天然油和脂肪（包括二十碳五烯酸或其衍生物）衍生的脂肪酸或其酯的混合物在高真空下使用多个蒸馏塔，特别是三个蒸馏塔或更多的蒸馏塔进行精馏，以获得 主要由碳数为20的脂肪酸或其酯组成的级分，然后用反相分配系统进行柱层析。 产生可用于预防或治疗血栓的二十碳五烯酸或其酯，纯度高达99％以上，回收率为55％以上。
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