公开(公告)号：US20060234980A1

公开(公告)日：2006-10-19

申请号：US10516234

申请日：2003-05-29

申请人： Hiroyuki Hashimoto ,  Kei Sonoyama ,  Koki Fujita ,  Kozo Hara ,  Masamichi Okada ,  Shigeharu Mori

发明人： Hiroyuki Hashimoto ,  Kei Sonoyama ,  Koki Fujita ,  Kozo Hara ,  Masamichi Okada ,  Shigeharu Mori

IPC分类号： A61K31/716 ,  A61K31/715 ,  A61K47/00

CPC分类号： C07H3/06 ,  A23L33/10 ,  A61K31/702 ,  A61K31/715

摘要： It is intended to provide an anti-inflammatory agent made from a highly safe material which can be daily taken as a food, an agent for preventing or ameliorating allergic diseases and a functional food having the above-mentioned effects. The anti-inflammatory agent contains an α-bond galactooligosaccharide as the active ingredient. The agent for preventing or ameliorating allergic diseases contains an α-bond galactooligosaccharide as the active ingredient. The functional food contains an α-bond galactooligosaccharide as the active ingredient. As such α-bond galactooligosaccharide, use can be made of a product synthesized by the dehydration condensation reaction by α-galactosidase with the use of galactose or galactose-containing substance as a substrate.

摘要翻译： 本发明提供一种由日常服用的高度安全的材料制成的抗炎剂，用于预防或改善过敏性疾病的药剂和具有上述效果的功能性食品。 抗炎剂含有α-键低聚半乳糖作为活性成分。 用于预防或改善过敏性疾病的药物含有α-键低聚半乳糖作为活性成分。 功能性食品含有α-键低聚半乳糖作为活性成分。 作为这样的α-键低聚半乳糖，可以使用通过使用半乳糖或含半乳糖的物质作为底物的α-半乳糖苷酶通过脱水缩合反应合成的产物。

公开(公告)号：US06284510B1

公开(公告)日：2001-09-04

申请号：US09503172

申请日：2000-02-14

申请人： Tetsuya Ito ,  Koki Fujita ,  Kozo Hara ,  Takashi Tonozuka ,  Yoshiyuki Sakano

发明人： Tetsuya Ito ,  Koki Fujita ,  Kozo Hara ,  Takashi Tonozuka ,  Yoshiyuki Sakano

IPC分类号： C12P2106

CPC分类号： C12N9/2408 ,  C12N9/2431 ,  C12Y302/01026

摘要： A &bgr;-fructofuranosidase gene coding for a protein having an amino acid sequence described in Seq. I.D. No. 1 in the Sequence Listing. The gene enables production of &bgr;-fructofuranosidase on a large scale more easily and less dependently on the productivity of microorganisms than the production by cultivation of the microorganisms. Also, the &bgr;-fructofuranosidase gene is useful for the development of variant enzymes that have increased heat resistance and transfer ratio by means of genetic engineering techniques.

摘要翻译： 编码具有Seq。所述的氨基酸序列的蛋白质的β-呋喃果糖苷酶基因。 ID。 序列表第1号。 该基因能够比通过微生物培养的生产更容易且更不依赖于微生物的生产力而大量生产β-呋喃果糖苷酶。 此外，β-呋喃果糖苷酶基因可用于通过遗传工程技术开发具有增加的耐热性和转移比的变体酶。

公开(公告)号：US5523218A

公开(公告)日：1996-06-04

申请号：US368392

申请日：1994-12-30

申请人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Kyoko Koizumi

发明人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Kyoko Koizumi

IPC分类号： C08B37/16 ,  C12P19/18 ,  C12P19/04 ,  C12P19/08 ,  C12P19/44

CPC分类号： A61Q19/00 ,  A61K8/738 ,  C08B37/0012 ,  C12P19/18

摘要： Disclosed are (1) a novel galactosyl-cyclodextrin having a galactosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an .alpha.-bond and a method of producing said galactosyl-cyclodextrin; (2) a novel galactosyl-cyclodextrin having a galactosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via a .beta.-bond and a method of producing said galactosyl-cyclodextrin; and (3) a novel mannosyl-cyclodextrin having a mannosyl group as bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an .alpha.-bond and a method of producing said mannosyl-cyclodextrin. The new branched cyclodextrins are expected to be widely useful in various fields of drug industry, food industry and cosmetic industry.

摘要翻译： 公开了（1）具有通过α键与环糊精的葡糖基的羟基键合的半乳糖基的新型半乳糖基 - 环糊精和所述半乳糖基 - 环糊精的制备方法; （2）具有通过β键与环糊精的葡糖基的羟基键合的半乳糖基的新型半乳糖基 - 环糊精和所述半乳糖基 - 环糊精的制造方法; 和（3）具有通过α键与环糊精的葡糖基的羟基键合的甘露糖基的新型甘露糖基 - 环糊精和所述甘露糖基 - 环糊精的制造方法。 新型支链环糊精预期在药物工业，食品工业和化妆品行业的各个领域中广泛应用。

公开(公告)号：US5393660A

公开(公告)日：1995-02-28

申请号：US147717

申请日：1993-11-04

申请人： Sumio Kitahata ,  Nobuhiro Kuwahara ,  Koki Fujita ,  Koji Hara ,  Keiichi Majima ,  Shin-ichi Teshima ,  Yuzo Hayashi

发明人： Sumio Kitahata ,  Nobuhiro Kuwahara ,  Koki Fujita ,  Koji Hara ,  Keiichi Majima ,  Shin-ichi Teshima ,  Yuzo Hayashi

IPC分类号： C07H15/203 ,  C12Q1/40 ,  C12N9/26

CPC分类号： C07H15/203 ,  C12Q1/40 ,  C12Q2334/00 ,  C12Q2334/10

摘要： A reagent for determining a-amylase activity, comprising a maltooligosaccharide derivative of the following formula ##STR1## (wherein either one of R.sub.1 and R.sub.2 is .beta.-galactopyranosil and the other is hydrogen, R.sub.3 is a group bonded to the reducing terminal glucose via a bond cleavable by .alpha.-amylase, which becomes a measurable substance upon cleavage of the bond, and n is an integer of 0-2), which does not comprise adjuvant enzymes; and a method for determining .alpha.-amylase activity which comprises use of the reagent. The reagent of the present invention does not require use of any adjuvant enzyme and is stable since the substrate is not exposed to the decomposition by an adjuvant enzyme. The substrate used in the present invention has high affinity for .alpha.-amylase. Thus, the reagent and the determination method of the present invention make it possible to determine .alpha.-amylase activity with high sensitivity.

摘要翻译： 一种用于测定α-淀粉酶活性的试剂，包括下式的麦芽低聚糖衍生物（Ⅰ）（其中R 1和R 2中的任一个是β-半乳糖吡喃糖苷，另一个是氢，R 3是键合到还原末端的基团 葡萄糖通过可以被α-淀粉酶切割的键，其在切割键时成为可测量物质，n是0-2的整数），其不包含辅酶; 以及一种测定α-淀粉酶活性的方法，其包括使用该试剂。 本发明的试剂不需要使用任何辅助酶，并且是稳定的，因为底物不被辅助酶暴露于分解。 用于本发明的底物对α-淀粉酶具有高亲和力。 因此，本发明的试剂和测定方法能够以高灵敏度确定α-淀粉酶活性。

公开(公告)号：US5366879A

公开(公告)日：1994-11-22

申请号：US992862

申请日：1992-12-17

申请人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Hirofumi Nakano

发明人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Hirofumi Nakano

IPC分类号： C12P19/16 ,  C12P19/18 ,  C12P19/22

CPC分类号： C12P19/18

摘要： Disclosed is a method of producing a branched cyclodextrin where a liquid containing a cyclodextrin and a branched oligosaccharide is treated with a debranching enzyme. By the method, a branched cyclodextrin having a maltosyl or maltotriosyl group as bonded to the glucose of a CD molecule via an .alpha.-1,6 bond is obtained efficiently.

摘要翻译： 公开了一种制备支链环糊精的方法，其中用脱支酶处理含有环糊精和支链寡糖的液体。 通过该方法，有效地获得具有通过α-1,6键与CD分子的葡萄糖结合的麦芽糖基或麦芽三糖基的支链环糊精。

公开(公告)号：US5089401A

公开(公告)日：1992-02-18

申请号：US672388

申请日：1991-03-20

申请人： Koki Fujita ,  Kozo Hara ,  Hitoshi Hashimoto ,  Sumio Kitahata

发明人： Koki Fujita ,  Kozo Hara ,  Hitoshi Hashimoto ,  Sumio Kitahata

IPC分类号： C12P19/14 ,  C07H3/06 ,  C12N9/10 ,  C12N9/26 ,  C12P19/12 ,  C12P19/18 ,  C12P19/24 ,  C12R1/06

CPC分类号： C12N9/2408 ,  C12P19/12 ,  C12P19/24 ,  Y10S435/83

摘要： An enzymatic method for the preparation of a fructose-containing oligosaccharide, in which a .beta.-fructofuranosidase obtained by culturing Arthrobacter sp. K-1 (FERM BP-3192) as an enzyme is reacted on sucrose, raffinose or stachyose as the donor in the presence of an aldose or ketose as the receptor. The enzyme is characterized by:(1) activity on sucrose for the transglycosidation of a fructosyl group to the receptor in the presence of a monosaccharide, sugar alcohol, alkyl alcohol, glycoside or oligosaccharide;(2) activity for the decomposition of sucrose, elrose, neokestose, xylsucrose, raffinose and stachyose with inactivity on a saccharide selected from the group consisting of 1-kestose, nistose, inulobiose and levan biose;(3) optimum pH of 6.5 to 6.8 at 40.degree. C. with stability at a pH of 5.5 to 10;(4) optimum temperature of 55.degree. C. at a pH of 6.5 exhibiting at least 70% of residual activity at 60.degree. C.;(5) susceptibility to inhibition by the ions of silver, mercury, zinc, copper and tin;(6) two molecular weights of 52,000.+-.2,500 and 58,000.+-.2,500; and(7) two isoelectric points of pH 4.3 and pH 4.6.

公开(公告)号：US07491518B2

公开(公告)日：2009-02-17

申请号：US10362483

申请日：2001-08-08

申请人： Masamichi Okada ,  Shigeharu Mori ,  Hiroyuki Hashimoto ,  Koki Fujita ,  Kozo Hara

发明人： Masamichi Okada ,  Shigeharu Mori ,  Hiroyuki Hashimoto ,  Koki Fujita ,  Kozo Hara

IPC分类号： C12P19/14 ,  C12P19/12 ,  C12P19/18

CPC分类号： C12N9/2465 ,  C12P19/14 ,  C12Y302/01022

摘要： A novel method of very efficiently elevating the yield of oligosacchrides containing α-galactosyl, compared with the conventional methods, which comprises treating galactose or a galactose-containing material with a specific α-galactosidase and thus performing a dehydrocondensation reaction at a high substrate concentration. Anti-candida compositions originating in foods, having a high safety and excellent anti-candida effect and not being restricted in the supply, which contain as the active ingredient oligosacchrides obtained by treating galactose or a galactose-containing material with an α-galactosidase and thus performing a dehydrocondensation.

摘要翻译： 与常规方法相比，非常有效地提高含有α-半乳糖苷的寡糖的产率的新方法，其包括用特定的α-半乳糖苷酶处理半乳糖或含半乳糖的材料，从而以高底物浓度进行脱氢反应。 起始于食物的抗念珠菌组合物，具有高安全性和优异的抗念珠菌作用，不受供体限制，其含有通过用α-半乳糖苷酶处理半乳糖或含半乳糖的物质获得的活性成分的寡糖，因此 进行脱氢。

公开(公告)号：US5763598A

公开(公告)日：1998-06-09

申请号：US876979

申请日：1997-06-16

申请人： Kenichi Hamayasu ,  Tetsuya Ito ,  Koki Fujita ,  Kozo Hara ,  Kyoko Koizumi ,  Toshiko Tanimoto ,  Hirofumi Nakano ,  Sumio Kitahata

发明人： Kenichi Hamayasu ,  Tetsuya Ito ,  Koki Fujita ,  Kozo Hara ,  Kyoko Koizumi ,  Toshiko Tanimoto ,  Hirofumi Nakano ,  Sumio Kitahata

IPC分类号： C12P19/26 ,  A61K47/48 ,  C08B37/16 ,  A01N43/04 ,  A61K31/715

CPC分类号： B82Y5/00 ,  A61K47/48969 ,  C08B37/0012

摘要： Disclosed are novel branched cyclodextrins having any of an N-acetylglucosamine group, a glucosaminyl group, an N-acetylgalactosaminyl group and a galactosaminyl group as bonded to the hydroxyl group of the cyclodextrin ring by .beta.-bonding therebetween, and their salts. The novel branched cyclodextrins are produced by reacting a solution containing a cyclodextrin and an N-acetylglucosamine or N-acetylgalactosamine with an N-acetylhexosaminidase, and optionally deacetylating the resulting product.

摘要翻译： 公开了通过β键连接在环糊精环的羟基上的N-乙酰葡糖胺基，葡糖胺基，N-乙酰半乳糖胺基和半乳糖胺基中的任一种的新型支链环糊精及其盐。 新型支链环糊精是通过使含有环糊精和N-乙酰葡糖胺或N-乙酰半乳糖胺的溶液与N-乙酰基己糖胺酶反应，并任选地将所得产物脱乙酰化来制备的。

公开(公告)号：US5622844A

公开(公告)日：1997-04-22

申请号：US457091

申请日：1995-06-01

申请人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Kyoko Koizumi

发明人： Sumio Kitahata ,  Koji Hara ,  Koki Fujita ,  Nobuhiro Kuwahara ,  Kyoko Koizumi

IPC分类号： C08B37/16 ,  C12P19/18 ,  C12P19/04 ,  C12P19/08 ,  C12P19/44

CPC分类号： A61Q19/00 ,  A61K8/738 ,  C08B37/0012 ,  C12P19/18

摘要： A method of producing a mannosyl-cyclodextrin having a mannosyl group bonded to the hydroxyl group of the glucosyl group of a cyclodextrin via an alpha-bond. The method includes treating a liquid containing a cyclodextrin and an alpha-mannosyl compound with an alpha-mannosyl transfer agent.

摘要翻译： 具有通过α-键与环糊精的葡糖基的羟基键合的甘露糖基的甘露糖基 - 环糊精的制造方法。 该方法包括用α-甘露糖基转移剂处理含有环糊精和α-甘露糖基化合物的液体。

公开(公告)号：US20100075381A1

公开(公告)日：2010-03-25

申请号：US12598456

申请日：2008-04-21

申请人： Tetsuya Ito ,  Yuki Ario ,  Eriko Kishino ,  Koki Fujita

发明人： Tetsuya Ito ,  Yuki Ario ,  Eriko Kishino ,  Koki Fujita

IPC分类号： C12P19/00 ,  C12N1/21

CPC分类号： C12P19/02 ,  C12N1/20 ,  C12P17/04 ,  C12R1/01

摘要： The object of the present invention is to provide a microorganism having an excellent ability to specifically oxidize the hydroxymethyl group of glucose, and a method of producing glucuronic acid and/or glucuronolactone by using such a microorganism to directly oxidize glucose, and the invention is directed at a microorganism which produces glucuronic acid directly from glucose and a mutant strain which is capable of specifically oxidizing the hydroxymethyl group of glucose and in which a DNA nucleotide sequence corresponding to 16S rRNA has the nucleotide sequence of SEQ ID NO: 1 in the sequence listing, and the invention is also directed at a method of producing glucuronic acid and/or glucuronolactone by specifically oxidizing the hydroxymethyl group of glucose using the mutant strain, and this invention enables D-glucuronic acid and/or D-glucuronolactone to be produced and furnished easily and safely at a high yield and a low cost.

摘要翻译： 本发明的目的是提供一种具有优异的葡萄糖羟甲基氧化能力的微生物，以及通过使用这样的微生物直接氧化葡萄糖来生产葡萄糖醛酸和/或葡糖醛酸内酯的方法，本发明是针对 在从葡萄糖直接生产葡萄糖醛酸的微生物和能够特异性氧化葡萄糖羟甲基的突变菌株，其中对应于16S rRNA的DNA核苷酸序列具有序列表中SEQ ID NO：1的核苷酸序列 本发明还涉及通过使用突变菌株特异性氧化葡萄糖羟甲基来生产葡萄糖醛酸和/或葡糖醛酸内酯的方法，本发明使D-葡萄糖醛酸和/或D-葡糖醛酸内酯能够生产和配制 轻松安全地以高产量和低成本。