Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
    2.
    发明申请
    Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 失效
    分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法

    公开(公告)号:US20060040342A1

    公开(公告)日:2006-02-23

    申请号:US10920625

    申请日:2004-08-17

    摘要: The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.

    摘要翻译: 本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子,其表现出相对于表现出第二形态的天然真菌的第一形态。 本发明还包括利用真菌进行蛋白质或化学生产的方法。 通过用重组多核苷酸分子转化真菌产生转化的真菌。 重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。 基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达,以在转基因真菌中最佳产生感兴趣的化合物。