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公开(公告)号：US20190233477A1
公开(公告)日：2019-08-01
申请号：US16093943
申请日：2017-04-13
发明人： Roelof Ary Lans Bovenberg , Ulrike Maria Muller , Arnold Jacob Mathieu Driessen , Pohl Carsten , Reto Daniel Zwahlen
摘要： The present invention relates to a method to improve the production of a secondary metabolite catalyzed by a non-ribosomal peptide synthetase comprising contacting in a eukaryotic host a eukaryotic non-ribosomal peptide synthetase with an MbtH-like protein. The present invention further relates to a composition comprising a eukaryotic non-ribosomal peptide synthetase that is not a hybrid and a prokaryotic MbtH and to a eukaryotic host cell comprising a non-ribosomal peptide synthetase and a polynucleotide allowing the expression of an MbtH-like protein.
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公开(公告)号：US09657301B2
公开(公告)日：2017-05-23
申请号：US14980425
申请日：2015-12-28
申请人： DSM IP ASSETS B.V.
IPC分类号： C12N1/14 , C12N15/00 , C12Q1/68 , C12N9/00 , C12P21/04 , C07H21/04 , C07K1/00 , C12N15/80 , C07K14/37 , C12N15/90 , C12P21/02 , C12P23/00 , C12P37/00 , C12P17/10 , C12P21/00
CPC分类号： C12N15/80 , C07K14/37 , C12N15/902 , C12P17/10 , C12P21/00 , C12P21/02 , C12P23/00 , C12P37/00
摘要： The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.
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3.发明申请公开(公告)号：US20160362715A1
公开(公告)日：2016-12-15
申请号：US15252636
申请日：2016-08-31
CPC分类号： C12P37/00 , C07K5/0806 , C12N9/0004 , C12N15/70 , C12Y121/03001
摘要： The present invention relates to the preparation of β-lactam antibodies comprising contacting 4-hydroxyphenylglycine of phenylglycine, cysteine and valine with a non-ribosomal peptide synthetase and subsequent cyclization using an isopenicillin N synthase in the presence of an MbtH-like protein and to a host cell equipped to perform such preparation.
摘要翻译： 本发明涉及β-内酰胺抗体的制备，包括使苯基甘氨酸，半胱氨酸和缬氨酸的4-羟基苯基甘氨酸与非核糖体肽合成酶接触，随后在MbtH样蛋白质存在下使用异青霉素N合酶进行环化， 宿主细胞配备进行这样的准备。
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4.发明申请公开(公告)号：US20140349340A1
公开(公告)日：2014-11-27
申请号：US14374137
申请日：2013-01-28
IPC分类号： C07K5/083
CPC分类号： C12P37/00 , C07K5/0806 , C12N9/0004 , C12N15/70 , C12Y121/03001
摘要： The present invention relates to the preparation of β-lactam antibiotics comprising contacting 4-hydroxyphenylglycine or phenylglycine, cysteine and valine with a non-ribosomal peptide synthetase and subsequent cyclization using an isopenicillin N synthase in the presence of an MbtH-like protein and to a host cell equipped to perform such preparation.
摘要翻译： 本发明涉及β-内酰胺抗生素的制备，其包括使4-羟基苯基甘氨酸或苯基甘氨酸，半胱氨酸和缬氨酸与非核糖体肽合成酶接触，随后在MbtH样蛋白质存在下使用异青霉素N合酶进行环化， 配备进行这种准备的宿主细胞。
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公开(公告)号：US20110250656A1
公开(公告)日：2011-10-13
申请号：US12674752
申请日：2008-08-28
申请人： Sameer A.S. Mapari , Anne S. Meyer , Jens C. Frisvad , Ulf Thrane
发明人： Sameer A.S. Mapari , Anne S. Meyer , Jens C. Frisvad , Ulf Thrane
IPC分类号： C12P17/18 , C09D7/12 , C08K5/1545 , C08K5/3437 , A23L1/275 , C12P17/06 , C07D307/77 , C07D491/04 , C12M1/02 , D21H21/28 , C09D11/00
CPC分类号： C09B61/00 , A23C19/0682 , A23C19/0925 , A23L2/58 , A23L5/46 , C12P17/181 , C12P37/00
摘要： The present invention relates to the field of biotechnological production of polyketide based colorants from filamentous fungi, in particular a method for preparing a biomass comprising a Monascus-like pigment composition from a nontoxigenic and non-pathogenic fungal source. The present invention further relates to use of the Monascus-like pigment composition as a colouring agent for food items and/or non-food items, and a cosmetic composition comprising the Monascus-like pigment composition.
摘要翻译： 本发明涉及从丝状真菌生物技术生产基于聚酮化合物的着色剂的领域，特别是一种制备生物质的方法，所述生物质包含来自无毒性和非致病性真菌来源的红曲霉样颜料组合物。 本发明还涉及红曲霉样颜料组合物作为食品和/或非食品的着色剂的用途，以及包含红曲霉素类颜料组合物的化妆品组合物。
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6.发明申请公开(公告)号：US20080227145A1
公开(公告)日：2008-09-18
申请号：US10594014
申请日：2005-03-31
CPC分类号： C12N15/80 , C07K14/37 , C12N15/902 , C12P17/10 , C12P21/00 , C12P21/02 , C12P23/00 , C12P37/00
摘要： The present invention relates to a method for increasing the efficiency of targeted integration of a polynucleotide to a pre-determined site into the genome of a filamentous fungal cell with a preference for NHR, wherein said polynucleotide has a region of homology with said pre-determined site, comprising steering an integration pathway towards HR. The present invention also relates to a mutant filamentous fungus originating from a parent cell, said mutant having an HR pathway with elevated efficiency and/or an NHR pathway with a lowered efficiency and/or a NHR/HR ratio with decreased efficiency as compared to said HR and/or NHR efficiency and/or NHR/HR ratio of said parent cell under the same conditions.
摘要翻译： 本发明涉及一种提高多核苷酸靶向整合到具有优选NHR的丝状真菌细胞基因组中的预定位点的效率的方法，其中所述多核苷酸具有与所述预先确定的同源性的区域 包括指导人力资源的整合途径。 本发明还涉及源自亲本细胞的突变丝状真菌，所述突变体具有提高效率的HR途径和/或具有降低的效率和/或NHR / HR比率的NHR途径，与所述 在相同条件下所述亲本细胞的HR和/或NHR效率和/或NHR / HR比。
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公开(公告)号：US20080096242A1
公开(公告)日：2008-04-24
申请号：US11661548
申请日：2005-08-31
申请人： Johan Sanders , Ruud Weusthuis , Andreas Mooibroek
发明人： Johan Sanders , Ruud Weusthuis , Andreas Mooibroek
CPC分类号： C12P7/56 , C12P1/00 , C12P7/46 , C12P7/48 , C12P7/54 , C12P7/58 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , C12P13/06 , C12P13/08 , C12P13/14 , C12P37/00
摘要： The present invention relates to the field of fermentation technology. In particular the invention relates to fermentation processes for the production of a first and a second fermentation product by a single production organism wherein the first product is in a more reduced state than the substrate and the second fermentation product is in a more oxidised state than the substrate yet in a less oxidised state than the final oxidation product CO2, such that the concurrent synthesis of the first and second product in the organism allows recycling of reducing power and can be performed under (partially) anaerobic conditions. The invention also relates to such processes in which the first and second fermentation products are capable of forming a(n) (insoluble) complex or salt.
摘要翻译： 本发明涉及发酵技术领域。 特别地，本发明涉及用于通过单一生产生物体生产第一和第二发酵产物的发酵方法，其中第一产物处于比底物更低的状态，而第二发酵产物的氧化状态比 底物仍然比最终氧化产物CO 2 2氧化较少的状态，使得生物体中第一和第二产物的同时合成允许再循环还原能力并且可以在（部分）厌氧 条件。 本发明还涉及这样的方法，其中第一和第二发酵产物能够形成（n）（不溶性）络合物或盐。
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8.发明授权Method for producing novel β-lactam antibiotic from protoplast fusion strain 有权从原生质体融合菌株生产新型β-内酰胺抗生素的方法公开(公告)号：US07241588B2
公开(公告)日：2007-07-10
申请号：US10657199
申请日：2003-09-09
申请人： Chin-Chu Chen , Ying-Shih Feng , Charng-Cherng Chyau , Ching-Nung Chen , Shih-Jeng Huang , Yen-Lien Chen , Hung-Ping Tseng , Wei-Hui Chung , Yi-Hsuan Chen
发明人： Chin-Chu Chen , Ying-Shih Feng , Charng-Cherng Chyau , Ching-Nung Chen , Shih-Jeng Huang , Yen-Lien Chen , Hung-Ping Tseng , Wei-Hui Chung , Yi-Hsuan Chen
摘要： The present invention provides a method for producing a novel β-lactam antibiotic from a protoplast fusion strain. The method is to fermentatively culture the protoplast fusion strain of Penicillium chrysogenum and Cephalosporium acremonium. The ferment filtrate is isolated, lyophilized, and extracted by acetone or acetone/methanol. The extract is concentrated by decompression, and then analyzed by preparation type HPLC to isolate the active antibiotic compound.
摘要翻译： 本发明提供了从原生质体融合菌株生产新的β-内酰胺抗生素的方法。 该方法是发酵培养产黄青霉和顶头孢霉的原生质体融合菌株。 分离发酵滤液，冻干，用丙酮/丙酮/甲醇萃取。 提取物通过减压浓缩，然后通过制备型HPLC分析来分离活性抗生素化合物。
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公开(公告)号：US20060263864A1
公开(公告)日：2006-11-23
申请号：US10309407
申请日：2002-12-03
申请人： Robert Busby , Brian Cali , Peter Hecht , Douglas Holtzman , Kevin Madden , Mary Maxon , G. Milne , Thea Norman , John Royer , Jeff Silva , Eric Summers , Lixin Zhang , Maria Mayorga , Toby Feibelman , Reed Doten
发明人： Robert Busby , Brian Cali , Peter Hecht , Douglas Holtzman , Kevin Madden , Mary Maxon , G. Milne , Thea Norman , John Royer , Jeff Silva , Eric Summers , Lixin Zhang , Maria Mayorga , Toby Feibelman , Reed Doten
摘要： Methods for improving secondary metabolite production by modulating certain genes involved in secondary metabolite production, including genes encoding CreA, AreA, GanB, Gna3, FadA, Gna1, Gpa1, RfeH, An09, PacC, Lys14, LovU, Ste7, Pde2, Nc1, Vps34 and fungal homologs thereof.
摘要翻译： 包括编码CreA，AreA，GanB，Gna3，FadA，Gna1，Gpa1，RfeH，An09，PacC，Lys14，LovU，Ste7，Pde2，Nc1，Vps34等基因的调控某些参与次级代谢产物的基因的次级代谢产物生成方法 及其真菌同系物。
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公开(公告)号：US06949356B1
公开(公告)日：2005-09-27
申请号：US09487558
申请日：2000-01-19
申请人： Robert Busby , Brian Cali , Peter Hecht , Doug Holtzman , Kevin Madden , Mary Maxon , Todd Milne , Thea Norman , John Royer , Sofie Salama , Amir Sherman , Jeff Silva , Eric Summers
发明人： Robert Busby , Brian Cali , Peter Hecht , Doug Holtzman , Kevin Madden , Mary Maxon , Todd Milne , Thea Norman , John Royer , Sofie Salama , Amir Sherman , Jeff Silva , Eric Summers
IPC分类号： C12N15/09 , A61K31/351 , A61K38/00 , A61K45/00 , A61P3/06 , A61P31/04 , A61P35/00 , A61P37/06 , C07D309/30 , C07K14/38 , C07K14/395 , C12N1/15 , C12N15/80 , C12P17/06 , C12P35/00 , C12P35/06 , C12P37/00 , C12R1/66 , C12R1/82 , C12P1/00 , C12P1/02
摘要： The invention relates to the production of secondary metabolites by fungi. More particularly, the invention relates to improvement of production of commercially important secondary metabolites by fungi. The invention provides methods for improving secondary metabolite production in a fungus, comprising modulating the expression of a gene involved in regulation of secondary metabolite production.
摘要翻译： 本发明涉及由真菌生产次级代谢物。 更具体地，本发明涉及通过真菌改善商业上重要的次级代谢物的生产。 本发明提供了改善真菌中次生代谢物产生的方法，包括调节参与次级代谢产物调节的基因的表达。
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