利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

13 条记录 (耗时 0.02 秒)

    System and method for producing beads
    1.
    发明授权
    System and method for producing beads 有权
    珠粒的制备方法

    公开(公告)号：US09192907B2

    公开(公告)日：2015-11-24

    申请号：US12745611

    申请日：2008-12-02

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Rene Oehlenschlaeger Holte ,  Brian Larsen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Rene Oehlenschlaeger Holte ,  Brian Larsen

    IPC分类号： B01J2/08 ,  B01J13/04

    CPC分类号： B01J2/08 ,  B01J13/046

    摘要： The present invention relates to a method for producing beads comprising a material capable of gelation, said method comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilizing the droplets by transferring the emulsion from the first reactor to a stabilization reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.

    摘要翻译： 本发明涉及包含能够凝胶化的材料的珠粒的制造方法，所述方法包括以下步骤：（i）将（a）包含能够凝胶化的材料的液体组合物组合; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经过添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够 凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经受凝胶化手段，以在5分钟以内获得凝胶化，并形成珠粒。

    Isolation of immunoglobulins
    3.
    发明授权
    Isolation of immunoglobulins 有权
    免疫球蛋白的分离

    公开(公告)号：US07745582B2

    公开(公告)日：2010-06-29

    申请号：US11930556

    申请日：2007-10-31

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    IPC分类号： C07K1/22 ,  C07K16/00

    CPC分类号： C07K16/00 ,  B01D15/1807 ,  B01D15/3804 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3253 ,  B01J20/3255 ,  C07K1/22

    摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matrices, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

    摘要翻译： 本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。 杂交瘤细胞培养上清液，动物血浆或血清或初乳。 该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。 固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如， 羧酸。 所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。

    Isolation of immunoglobulins
    6.
    发明授权
    Isolation of immunoglobulins 有权
    免疫球蛋白的分离

    公开(公告)号：US06498236B1

    公开(公告)日：2002-12-24

    申请号：US09147668

    申请日：1999-03-19

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen

    IPC分类号： B01D1500

    CPC分类号： C07K16/00 ,  B01D15/1807 ,  B01D15/3804 ,  B01J20/3219 ,  B01J20/3242 ,  B01J20/3253 ,  B01J20/3255 ,  C07K1/22

    摘要： The present invention relates to a novel method for the isolation or purification of immunoglobulins (a special class of proteins) from a solution containing immunoglobulins, e.g. hybridoma cell culture supernatants, animal plasma or sera, or colostrum. The method includes the use of a minimum of salts, such as lyotropic salts, in the binding process and preferably also the use of small amounts of organic solvents in the elution process. The solid phase matrices, preferably epichlorohydrin activated agarose matricees, are functionalised with mono- or bicyclic aromatic or heteroaromatic ligands (molecular weight: at the most 500 Dalton) which, preferably, comprises an acidic substituent, e.g. a carboxylic acid. The matrices utilised show excellent properties in a “Standard Immunoglobulin Binding Test” and in a “Monoclonal Antibody Array Binding Test” with respect to binding efficiency and purity, and are stable in 1M NaOH.

    摘要翻译： 本发明涉及从含有免疫球蛋白的溶液中分离或纯化免疫球蛋白（一类特殊蛋白质）的新方法。 杂交瘤细胞培养上清液，动物血浆或血清或初乳。 该方法包括在结合过程中使用最少量的盐，例如溶性盐，并且还优选在洗脱过程中使用少量的有机溶剂。 固相基质，优选表氯醇活化的琼脂糖基质，用单环或双环芳族或杂芳族配体（分子量：最多500道尔顿）官能化，其优选包含酸性取代基，例如， 羧酸。 所使用的基质在结合效率和纯度方面在“标准免疫球蛋白结合试验”和“单克隆抗体阵列结合试验”中显示出优异的性质，并且在1M NaOH中稳定。

    SYSTEM AND METHOD FOR PRODUCING BEADS
    9.
    发明申请
    SYSTEM AND METHOD FOR PRODUCING BEADS 有权
    用于生产珠粒的系统和方法

    公开(公告)号：US20110018153A1

    公开(公告)日：2011-01-27

    申请号：US12745611

    申请日：2008-12-02

    申请人： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Rene Oehlenschlaeger Holte ,  Brian Larsen

    发明人： Allan Otto Fog Lihme ,  Marie Bendix Hansen ,  Rene Oehlenschlaeger Holte ,  Brian Larsen

    IPC分类号： B29B9/00 ,  B01J2/08

    CPC分类号： B01J2/08 ,  B01J13/046

    摘要： The present invention relates to a method for producing beads comprising a material capable of gelation, said meth comprising the steps of: (i) combining (a) a liquid composition comprising a material capable of gelation; and (b) a first hydrophobic phase; (ii) subjecting the liquid composition and the first hydrophobic phase, to means for emulsification in a first reactor by addition of external mechanical energy creating an emulsion comprising individual droplets comprising the material capable of gelation in the first hydrophobic phase (wherein the material capable of gelation provides a discontinuous phase and the first hydrophobic phase provides a continuous phase); (iii) stabilising the droplets by transferring the emulsion from the first reactor to a stabilisation reactor wherein the emulsion obtained in step (ii) is subjected to means for gelation in order to obtain gelation within 5 minutes or less, and the beads are formed.

    摘要翻译： 本发明涉及包含能够凝胶化的材料的珠粒的生产方法，所述的方法包括以下步骤：（i）组合（a）包含能凝胶化的材料的液体组合物; 和（b）第一疏水相; （ii）使液体组合物和第一疏水相经过添加外部机械能在第一反应器中进行乳化的装置，产生包含单个液滴的乳液，所述液滴包含能够在第一疏水相中凝胶化的材料（其中能够 凝胶化提供不连续相，第一疏水相提供连续相）; （iii）通过将乳液从第一反应器转移到稳定反应器中来稳定液滴，其中在步骤（ii）中获得的乳液经历凝胶化手段以在5分钟或更短时间内获得凝胶化，并形成珠粒。

    ISOLATION AND SEPARATION OF MINIMALLY DENATURED POTATO PROTEINS AND PEPTIDES
    10.
    发明申请
    ISOLATION AND SEPARATION OF MINIMALLY DENATURED POTATO PROTEINS AND PEPTIDES 审中-公开
    最小去除的蛋白质和蛋白质的分离和分离

    公开(公告)号：US20100087628A1

    公开(公告)日：2010-04-08

    申请号：US12524817

    申请日：2008-01-29

    申请人： Allen Otto Fog Lihme ,  Marie Bendix Hansen ,  Martin Pontoppidan

    发明人： Allen Otto Fog Lihme ,  Marie Bendix Hansen ,  Martin Pontoppidan

    IPC分类号： C07K14/415 ,  C07K1/22

    CPC分类号： C07K14/81 ,  C07K1/22 ,  C07K14/415

    摘要： The present invention relates to the large-scale fractionation and isolation of peptides, polypeptides and protein(s) from a potato derived protein solution such as potato extract, potato fruit juice and fruit water using an adsorbent coupled with a ligand for the capture of the protein(s), from the protein solution. In particular the invention relates to a process for the isolation and separation of patatin and potato protease inhibitors using a low temperature non-denaturing process.

    摘要翻译： 本发明涉及使用与配体偶联的吸附剂从马铃薯衍生的蛋白质溶液如马铃薯提取物，马铃薯果汁和水果水中大量分级分离和分离肽，多肽和蛋白质，以捕获 蛋白质，从蛋白质溶液中。 特别地，本发明涉及使用低温非变性方法分离和分离patatin和马铃薯蛋白酶抑制剂的方法。