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1.发明申请公开(公告)号：US20120244595A1
公开(公告)日：2012-09-27
申请号：US13059956
申请日：2009-08-19
CPC分类号： C12N9/14 , C07K2319/01 , C07K2319/035 , C12N9/2462 , C12Y302/01017
摘要： The present invention relates to recombinant polypeptides having the activity of binding and lysing of bacteria, comprising at least one enzymatically active domain and at least two bacterial cell binding domains. The present invention further relates to recombinant polypeptide having the activity of binding bacteria, comprising at least two bacterial cell binding domain. Further the present inventions relates to nucleic acid molecules comprising a nucleotide sequence encoding the recombinant polypeptides, vectors and host cells.
摘要翻译： 本发明涉及具有结合和裂解细菌活性的重组多肽，其包含至少一个酶活性结构域和至少两个细菌细胞结合结构域。 本发明还涉及具有结合细菌活性的重组多肽，其包含至少两个细菌细胞结合结构域。 此外，本发明涉及包含编码重组多肽，载体和宿主细胞的核苷酸序列的核酸分子。
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公开(公告)号：US20050153415A1
公开(公告)日：2005-07-14
申请号：US10503556
申请日：2003-01-22
申请人： Markus Zimmer , Martin Loessner , Andrew Morgan
发明人： Markus Zimmer , Martin Loessner , Andrew Morgan
IPC分类号： G01N33/569 , A61K38/00 , A61K38/46 , A61P1/00 , A61P31/04 , C07K14/01 , C12N1/21 , C12N9/78 , C12N15/09 , C12Q1/04 , C12Q1/34 , C12N9/24 , C07H21/04 , C12N7/00 , C12N15/74
CPC分类号： C07K14/005 , A61K38/00 , C12N2795/10022
摘要： A nucleic acid comprising a nucleotide sequence encoding a lysin, which nucleotide sequence comprises the sequence shown in SEQ IDNo. 1 or a variant, homologue or derivative thereof. A novel lysin obtainable from a bacteriophage capable of colonising Clostridium perfringens, and the use thereof as a medicament for the treatment of a disorder associated with pathogenic Clostridium bacteria, for example.
摘要翻译： 包含编码赖氨酸的核苷酸序列的核酸，所述核苷酸序列包含SEQ ID NO：所示的序列。 1或其变体，同系物或其衍生物。 可从能够定殖产气荚膜梭菌的噬菌体获得的新型赖氨酸及其作为用于治疗与病原性梭菌属细菌相关的病症的药物的用途。
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公开(公告)号：US20140287433A1
公开(公告)日：2014-09-25
申请号：US14233740
申请日：2012-07-18
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , G01N33/5432 , G01N2333/33 , G01N2333/952
摘要： This invention relates to a method of determining presence, amount and/or activity of a clostridial neurotoxin in a sample, the method comprising or consisting of the following steps: (a) bringing said sample into contact with a liposome, said liposome comprising (aa) at least one receptor on its outer surface, said receptor being capable of binding said neurotoxin and comprising or consisting of (i) a glycolipid and (ii) a peptide or protein; and (ab) a substrate in its interior, said substrate (i) being cleavable by the peptidase comprised in said neurotoxin and (ii) generating a detectable signal upon cleavage, said detectable signal preferably being generated by (1) the donor of a FRET pair, said donor exhibiting increased fluorescence upon cleavage by said peptidase, (2) a luminescent compound formed upon said cleavage, or (3) an enzyme formed upon said cleavage; and (b) determining whether an increase in signal occurs as compared to the absence of said sample, wherein such increase is indicative of the presence of said neurotoxin and/or the degree of such increase is indicative of the amount and/or activity of said neurotoxin in said sample.
摘要翻译： 本发明涉及一种测定样品中梭菌神经毒素的存在，量和/或活性的方法，所述方法包括或由以下步骤组成：（a）使所述样品与脂质体接触，所述脂质体包含（aa ）在其外表面上的至少一个受体，所述受体能够结合所述神经毒素并且包含（i）糖脂和（ii）肽或蛋白质或由其组成; 和（ab）其内部的底物，所述底物（i）可由包含在所述神经毒素中的肽酶切割，和（ii）在切割时产生可检测信号，所述可检测信号优选通过（1）FRET的供体产生 所述供体在所述肽酶切割时表现出增加的荧光，（2）在所述切割时形成的发光化合物，或（3）在所述切割时形成的酶; 和（b）确定与不存在所述样品相比是否发生信号增加，其中这种增加指示所述神经毒素的存在和/或这种增加的程度指示所述神经毒素的量和/或活性 所述样品中的神经毒素。
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公开(公告)号：US20140079727A1
公开(公告)日：2014-03-20
申请号：US13055646
申请日：2009-07-24
CPC分类号： C12N9/2402 , A01N63/00 , A23B4/20 , A23L3/3526 , A23L3/3571 , A61K38/47 , A61K45/06 , C12N9/2462
摘要： The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO:1. The present invention further relates to the nucleic acid molecules comprising a nucleotide sequence coding for the polypeptide, vectors comprising the nucleic acid molecules, and host cells for the expression of the polypeptides. In addition, the present invention relates to the use of the polypeptide as a human medical, veterinary medical or diagnostic substance, as an antimicrobial substance in food, in cosmetics, as disinfecting agent or in the environmental field.
摘要翻译： 本发明涉及具有根据SEQ ID NO：1的氨基酸序列的多肽。 本发明还涉及包含编码多肽的核苷酸序列，包含核酸分子的载体和用于表达多肽的宿主细胞的核酸分子。 此外，本发明涉及多肽作为人类医学，兽医医学或诊断物质，作为食品中的抗微生物物质，在化妆品中，作为消毒剂或在环境领域中的用途。
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公开(公告)号：US07371375B2
公开(公告)日：2008-05-13
申请号：US10503556
申请日：2003-01-22
CPC分类号： C07K14/005 , A61K38/00 , C12N2795/10022
摘要： A nucleic acid comprising a nucleotide sequence encoding a lysin, which nucleotide sequence comprises the sequence shown in SEQ ID No. 1 or a variant, homologue or derivative thereof. A novel lysin obtainable from a bacteriophage capable of colonising Clostridium perfringens, and the use thereof as a medicament for the treatment of a disorder associated with pathogenic Clostridium bacteria, for example.
摘要翻译： 包含编码赖氨酸的核苷酸序列的核酸，所述核苷酸序列包含SEQ ID No.1所示的序列或其变体，同系物或衍生物。 可从能够定殖产气荚膜梭菌的噬菌体获得的新型赖氨酸及其作为用于治疗与病原性梭菌属细菌相关的病症的药物的用途。
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6.发明申请Virulent phages to control listeria monocytogenes in foodstuffs and in food processing plants 有权有毒的噬菌体在食品和食品加工厂中控制单核细胞增多性李斯特菌公开(公告)号：US20050175594A1
公开(公告)日：2005-08-11
申请号：US10516507
申请日：2003-07-07
申请人： Martin Loessner , Richard Carlton
发明人： Martin Loessner , Richard Carlton
IPC分类号： A01N63/00 , A23G9/30 , A23L3/3463 , A23L3/3571 , C07K14/01 , C12N7/00 , C12N9/14 , C12N9/50 , C12N15/74
CPC分类号： C07K14/005 , A23G9/30 , A23G9/305 , A23L3/34635 , A23L3/3571 , C12N7/00 , C12N9/14 , C12N9/503 , C12N15/74 , C12N2795/10122 , C12N2795/10143
摘要： The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and/or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and/or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and/or with other antimicrobial agents to reduce or eliminate Listeria.
摘要翻译： 本发明涉及来自病毒科（Myoviridae）的毒性（溶解性）单核细胞增生利斯特氏菌（Listeria monocytogenes）噬菌体，优选P100，单独或与其它有毒噬菌体组合。 P100和来自P100的细胞内溶素可以施用于食品，将被添加到食品中的组分和/或对这些食品加工的食品加工厂的基础设施，或其中的容器或包装 这些食物被储存和/或运输，以便减少单核细胞增生利斯特氏菌的污染。 本发明中也可以使用P100来鉴别存在于食品中（或内部）的单核细胞增生李斯特细菌，以及存在于食品加工厂的设备或一般环境中的单核细胞增生李斯特菌，其中正在处理食品， 在感染单核细胞增多性李斯特菌的动物体内。 本发明的噬菌体和细胞内溶素也可用于治疗感染单核细胞增多性李斯特菌的动物。 P100将以很高的效率杀死其主体范围内的细菌，并在其上传播高滴度。 P100可以与其他溶解性噬菌体和/或与其他抗微生物剂组合以减少或消除李斯特菌。
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公开(公告)号：US5824468A
公开(公告)日：1998-10-20
申请号：US648978
申请日：1996-05-17
申请人： Siegfried Scherer , Martin Loessner
发明人： Siegfried Scherer , Martin Loessner
CPC分类号： C12N15/86 , C12N15/77 , C12N7/00 , C12Q1/04 , C12N2795/10143
摘要： The invention relates to a detection procedure for bacteria of the genus Listeria, comprising the steps: (a) provision of a DNA vector prepared by means of recombination techniques, comprising a genetic system comprising DNA which encodes the expression of one or more proteins, the proteins not being a gene product of bacteria of the genus Listeria and it being possible to determine the presence of the proteins by a detection reaction, and the DNA vector infecting the bacteria of the genus Listeria and it being possible in this way to transfer the genetic system to the bacteria; (b) mixing of the sample with said DNA vector under conditions which allow an infection of bacteria of the genus Listeria by the DNA vector; (c) expression of the detectable proteins in the bacteria of the genus Listeria; (d) detection of the detectable proteins, the presence of bacteria of the genus Listeria being detected, and to recombinant DNA vectors and reagent compositions suitable for this detection procedure.
摘要翻译： 本发明涉及利斯特氏菌属细菌的检测方法，包括以下步骤：（a）提供通过重组技术制备的DNA载体，其包含编码一种或多种蛋白质表达的DNA的遗传系统， 蛋白质不是李斯特氏菌属的细菌的基因产物，并且可以通过检测反应来确定蛋白质的存在，并且感染李斯特氏菌的细菌的DNA载体可以以这种方式转移遗传 系统对细菌; （b）在允许DNA载体感染李斯特菌属细菌的条件下，将样品与所述DNA载体混合; （c）在李斯特氏菌属细菌中表达可检测的蛋白质; （d）可检测蛋白质的检测，检测到的李斯特菌属细菌的存在，以及适用于该检测程序的重组DNA载体和试剂组合物。
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8.发明申请公开(公告)号：US20120171188A1
公开(公告)日：2012-07-05
申请号：US13251896
申请日：2011-10-03
CPC分类号： G01N33/56911 , A01N63/02 , C07K2319/01 , C12N9/1048 , C12N9/48 , C12N9/80 , C12N9/88
摘要： The present invention relates to recombinant polypeptides having the activity of binding and lysing of bacteria, comprising at least one enzymatically active domain and at least two bacterial cell binding domains. The present invention further relates to recombinant polypeptide having the activity of binding bacteria, comprising at least two bacterial cell binding domain. Further the present inventions relates to nucleic acid molecules comprising a nucleotide sequence encoding the recombinant polypeptides, vectors and host cells.
摘要翻译： 本发明涉及具有结合和裂解细菌活性的重组多肽，其包含至少一个酶活性结构域和至少两个细菌细胞结合结构域。 本发明还涉及具有结合细菌活性的重组多肽，其包含至少两个细菌细胞结合结构域。 此外，本发明涉及包含编码重组多肽，载体和宿主细胞的核苷酸序列的核酸分子。
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公开(公告)号：US20120128652A1
公开(公告)日：2012-05-24
申请号：US13230642
申请日：2011-09-12
IPC分类号： A61K38/48 , C12N15/57 , A61P29/00 , C12N1/00 , G01N33/569 , A61P31/04 , C12N9/50 , C12N15/63
CPC分类号： C12N9/2462 , A61K38/00 , C12N1/06 , C12Y302/01017 , G01N33/56911
摘要： The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO:1. The present invention further relates to the nucleic acid molecules comprising a nucleotide sequence coding for the polypeptide, vectors comprising the nucleic acid molecules, and host cells for the expression of the polypeptides. In addition, the present invention relates to the use of the polypeptide as a human medical, veterinary medical or diagnostic substance, as an antimicrobial substance in food, in cosmetics, as disinfecting agent or in the environmental field.
摘要翻译： 本发明涉及具有根据SEQ ID NO：1的氨基酸序列的多肽。 本发明还涉及包含编码多肽的核苷酸序列，包含核酸分子的载体和用于表达多肽的宿主细胞的核酸分子。 此外，本发明涉及多肽作为人类医学，兽医医学或诊断物质，作为食品中的抗微生物物质，在化妆品中，作为消毒剂或在环境领域中的用途。
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10.发明授权Virulent phages to detect Listeria monocytogenes in foodstuffs and in food processing plants 有权有毒的噬菌体检测食品中的单核细胞增多性李斯特菌和食品加工厂公开(公告)号：US08034552B2
公开(公告)日：2011-10-11
申请号：US12200375
申请日：2008-08-28
申请人： Martin Loessner , Richard Carlton
发明人： Martin Loessner , Richard Carlton
IPC分类号： C12Q1/70
CPC分类号： C07K14/005 , A23G9/30 , A23G9/305 , A23L3/34635 , A23L3/3571 , C12N7/00 , C12N9/14 , C12N9/503 , C12N15/74 , C12N2795/10122 , C12N2795/10143
摘要： The present invention relates to virulent (lytic) Listeria monocytogenes phage from the Myoviridae family, preferably P100, alone or in combination with other virulent phages. P100 and the endolysin from P100 can be administered to food products, to the components that will be added to food products, and/or to the infrastructure of the food processing plants within which such food products are processed, or the containers or wraps in which such foods are stored and/or shipped, in order to reduce Listeria monocytogenes contamination. P100 can also be used in the present invention to identify Listeria monocytogenes bacteria present on (or within) foodstuffs, as well as those Listeria monocytogenes bacteria present in the equipment or the general environment of the food processing plants in which the foodstuffs are being processed and in animals infected with Listeria monocytogenes. The phage and the endolysin of the present invention can also be used to treat animals infected with Listeria monocytogenes. P100 will kill the bacteria that are within its host range with great efficiency and will propagate to high titer thereon. P100 can be combined with other lytic phage, and/or with other antimicrobial agents to reduce or eliminate Listeria.
摘要翻译： 本发明涉及来自病毒科（Myoviridae）的毒性（溶解性）单核细胞增生利斯特氏菌（Listeria monocytogenes）噬菌体，优选P100，单独或与其它有毒噬菌体组合。 P100和来自P100的细胞内溶素可以施用于食品，将被添加到食品中的组分和/或对这些食品加工的食品加工厂的基础设施，或其中的容器或包装 这些食物被储存和/或运输，以便减少单核细胞增生利斯特氏菌的污染。 本发明中也可以使用P100来鉴别存在于食品中（或内部）的单核细胞增生李斯特细菌，以及存在于食品加工厂的设备或一般环境中的单核细胞增生李斯特菌，其中正在处理食品， 在感染单核细胞增多性李斯特菌的动物体内。 本发明的噬菌体和细胞内溶素也可用于治疗感染单核细胞增多性李斯特菌的动物。 P100将以很高的效率杀死其主体范围内的细菌，并在其上传播高滴度。 P100可以与其他溶解性噬菌体和/或与其他抗微生物剂组合以减少或消除李斯特菌。
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