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公开(公告)号：US06204030B1
公开(公告)日：2001-03-20
申请号：US09388805
申请日：1999-09-02
IPC分类号： C12P1912
CPC分类号： G02B21/32 , C12M33/02 , G01N1/04 , G01N1/08 , G01N1/2806 , G01N1/2813 , G01N1/286 , G01N1/31 , G01N15/1468 , G01N33/50 , G01N2001/028 , G01N2001/2833 , G01N2001/284 , G01N2035/00237 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.
摘要翻译： 一种显微切割的方法，其包括：使用显微镜形成组织样品的细胞的图像场，从细胞的图像区域鉴定感兴趣的细胞的至少一个区域，所述细胞的至少一个区域包括不同类型的细胞 而不是相邻的细胞区域，并且从组织样品中提取感兴趣的细胞的至少一个区域。 通过使组织样品与可以选择性活化的转移表面接触使得其区域粘附到待提取的感兴趣的细胞区域来实现提取。 转移表面包括可激发的粘合剂层，其向组织样品的选定区域提供化学或静电粘附。 转移表面被激活后，分离转移表面和组织样品。 在分离期间，感兴趣的细胞区域保持粘附到转移表面，并因此与组织样品分离。
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公开(公告)号：US5843657A
公开(公告)日：1998-12-01
申请号：US544388
申请日：1995-10-10
IPC分类号： G01N33/48 , C12M1/26 , C12M1/34 , G01N1/02 , G01N1/04 , G01N1/08 , G01N1/28 , G01N1/31 , G01N15/14 , G01N33/483 , G01N33/50 , G01N35/00 , G02B21/32 , A01N1/02 , A01N1/00 , A01N3/00
CPC分类号： G02B21/32 , C12M33/02 , G01N1/2813 , G01N1/286 , G01N33/50 , G01N1/04 , G01N1/08 , G01N1/2806 , G01N1/31 , G01N15/1468 , G01N2001/028 , G01N2001/2833 , G01N2001/284 , G01N2035/00237 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.
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公开(公告)号：US6010888A
公开(公告)日：2000-01-04
申请号：US925894
申请日：1997-09-08
IPC分类号： G01N33/48 , C12M1/26 , C12M1/34 , G01N1/02 , G01N1/04 , G01N1/08 , G01N1/28 , G01N1/31 , G01N15/14 , G01N33/483 , G01N33/50 , G01N35/00 , G02B21/32 , C12P19/12
CPC分类号： G02B21/32 , C12M33/02 , G01N1/2813 , G01N1/286 , G01N33/50 , G01N1/04 , G01N1/08 , G01N1/2806 , G01N1/31 , G01N15/1468 , G01N2001/028 , G01N2001/2833 , G01N2001/284 , G01N2035/00237 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.
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公开(公告)号：US06569639B2
公开(公告)日：2003-05-27
申请号：US09765937
申请日：2001-01-18
IPC分类号： G01N130
CPC分类号： G02B21/32 , C12M33/02 , G01N1/04 , G01N1/08 , G01N1/2806 , G01N1/2813 , G01N1/286 , G01N1/31 , G01N15/1468 , G01N33/50 , G01N2001/028 , G01N2001/2833 , G01N2001/284 , G01N2035/00237 , Y10T436/25 , Y10T436/25125 , Y10T436/25375
摘要： A method of microdissection which involves: forming an image field of cells of the tissue sample utilizing a microscope, identifying at least one zone of cells of interest from the image field of cells which at least one zone of cells of interest includes different types of cells than adjacent zones of cells, and extracting the at least one zone of cells of interest from the tissue sample. The extraction is achieved by contacting the tissue sample with a transfer surface that can be selectively activated so that regions thereof adhere to the zone of cells of interest to be extracted. The transfer surface includes an activatable adhesive layer which provides chemical or electrostatic adherence to the selected regions of the tissue sample. After the transfer surface is activated the transfer surface and tissue sample are separated. During separation the zone of cells of interest remains adhered to the transfer surface and is thus separated from the tissue sample.
摘要翻译： 一种显微切割的方法，其包括：使用显微镜形成组织样品的细胞的图像场，从细胞的图像区域鉴定感兴趣的细胞的至少一个区域，所述细胞的至少一个区域包括不同类型的细胞 而不是相邻的细胞区域，并且从组织样品中提取感兴趣的细胞的至少一个区域。 通过使组织样品与可以选择性活化的转移表面接触使得其区域粘附到待提取的感兴趣的细胞区域来实现提取。 转移表面包括可激发的粘合剂层，其向组织样品的选定区域提供化学或静电粘附。 转移表面被激活后，分离转移表面和组织样品。 在分离期间，感兴趣的细胞区域保持粘附到转移表面，并因此与组织样品分离。
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