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    Replication of hepatitis C virus genome and identification of virus having high infectivity
    4.
    发明授权
    Replication of hepatitis C virus genome and identification of virus having high infectivity 失效
    复制丙型肝炎病毒基因组和识别感染性高的病毒

    公开(公告)号：US5766919A

    公开(公告)日：1998-06-16

    申请号：US654128

    申请日：1996-05-28

    申请人： Hiroshi Yoshikura ,  Yohko Shimizu ,  Aikichi Iwamoto ,  Minako Hijikata

    发明人： Hiroshi Yoshikura ,  Yohko Shimizu ,  Aikichi Iwamoto ,  Minako Hijikata

    IPC分类号： C12N7/00 ,  C12Q1/04 ,  G01N33/50 ,  G01N33/576 ,  C12N7/01

    CPC分类号： G01N33/5767 ,  C12N7/00 ,  C12Q1/04 ,  G01N33/5091 ,  C12N2770/24221 ,  C12N2770/24251 ,  G01N2333/18

    摘要： A method for replication of hepatitis C virus genome comprising infecting animal cells that have been infected with mouse retrovirus, with hepatitis C virus, and then culturing those cells. The present invention further provides a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising centrifugating a sample comprising a virus to be tested in a liquid medium to determine the density of the virus, and assigning the virus to a hepatitis C virus with high infectivity it the density thereof is up to about 1.06 g/ml, and assigning the virus to a hepatitis C virus with less infectivity if the density thereof is at least 1.13 g/ml; a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising bringing a virus to be tested into contact with an anti-human immunoglobulin, and assigning the virus to hepatitis C virus with high infectivity if the virus does not form an immunocomplex and assigning the virus to a hepatitis C virus with less infectivity if it does form an immunocomplex; and a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising inoculating a virus to be tested to cells infected with murine retrovirus, culturing the cells, and assigning the virus to a hepatitis C virus with high infectivity if the virus replicates in the cells, or assigning the virus to a hepatitis C virus with less infectivity if the virus does not replicate in the cells.

    摘要翻译： 一种用于复制丙型肝炎病毒基因组的方法，包括用丙型肝炎病毒感染已被小鼠逆转录病毒感染的动物细胞，然后培养这些细胞。 本发明还提供了一种鉴别具有较高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法，包括在液体培养基中离心包含待测试病毒的样品以确定病毒的密度， 病毒感染性高的丙型肝炎病毒，其密度高达约1.06μg/ ml，并且如果其密度至少为1.13μg/ ml，则将该病毒分配给感染性较差的丙型肝炎病毒; 一种区分具有高感染性的丙型肝炎病毒和较少感染性的丙型肝炎病毒的方法，包括使待测试的病毒与抗人免疫球蛋白接触，并将病毒分配给感染性高的丙型肝炎病毒，如果病毒 如果它形成免疫复合物，则不形成免疫复合物并将病毒分配给具有较少感染性的丙型肝炎病毒; 以及用于区分具有高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法，包括将待测试的病毒接种到用鼠逆转录病毒感染的细胞，培养细胞，并将病毒分配给具有高度感染性的丙型肝炎病毒 如果病毒在细胞中复制，或者如果病毒不在细胞中复制，则将病毒分配给具有较少感染性的丙型肝炎病毒的感染性。

    Replication of hepatitis C virus genome and identification of virus having high infectivity
    6.
    发明授权
    Replication of hepatitis C virus genome and identification of virus having high infectivity 失效
    复制丙型肝炎病毒基因组和识别感染性高的病毒

    公开(公告)号：US5552310A

    公开(公告)日：1996-09-03

    申请号：US963490

    申请日：1992-10-20

    申请人： Hiroshi Yoshikura ,  Yohko Shimizu ,  Aikichi Iwamoto ,  Minako Hijikata

    发明人： Hiroshi Yoshikura ,  Yohko Shimizu ,  Aikichi Iwamoto ,  Minako Hijikata

    IPC分类号： C12N7/00 ,  C12Q1/04 ,  G01N33/50 ,  G01N33/576 ,  C12Q1/70 ,  C12N7/01 ,  C12N7/02

    CPC分类号： G01N33/5767 ,  C12N7/00 ,  C12Q1/04 ,  G01N33/5091 ,  C12N2770/24221 ,  C12N2770/24251 ,  G01N2333/18

    摘要： A method for replication of hepatitis C virus genome comprising infecting animal cells that have been infected with mouse retrovirus, with hepatitis C virus, and then culturing those cells. The present invention further provides a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising centrifugating a sample comprising a virus to be tested in a liquid medium to determine the density of the virus, and assigning the virus to a hepatitis C virus with high infectivity if the density thereof is up to about 1.06 g/ml, and assigning the virus to a hepatitis C virus with less infectivity if the density thereof is at least 1.13 g/ml; a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising brining a virus to be tested into contact with an anti-human immunoglobulin, and assigning the virus to hepatitis C virus with high infectivity if it does not form an immunocomplex and assigning the virus to a hepatitis C virus with less infectivity if it does form an immunocomplex; and a method for distinguishing a hepatitis C virus with high infectivity and a hepatitis C virus with less infectivity, comprising inoculating a virus to be tested to cells infected with murine retrovirus, culturing the cells, and assigning the virus to a hepatitis C virus with high infectivity if the virus replicates in the cells, or assigning the virus to a hepatitis C virus with less infectivity if the virus does not replicate in the cells.

    摘要翻译： 一种用于复制丙型肝炎病毒基因组的方法，包括用丙型肝炎病毒感染已被小鼠逆转录病毒感染的动物细胞，然后培养这些细胞。 本发明还提供了一种鉴别具有较高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法，包括在液体培养基中离心包含待测试病毒的样品以确定病毒的密度， 如果其密度高达约1.06μg/ ml，则具有高感染性的丙型肝炎病毒，如果其密度至少为1.13μg/ ml，则将病毒分配给具有较低感染性的丙型肝炎病毒; 一种区分具有高感染性的丙型肝炎病毒和较少感染性的丙型肝炎病毒的方法，包括将待测试的病毒与抗人免疫球蛋白接触，并将该病毒分配给感染性高的丙型肝炎病毒 不形成免疫复合物，如果确实形成免疫复合物，则将病毒分配给感染性较差的丙型肝炎病毒; 以及用于区分具有高感染性的丙型肝炎病毒和感染性较差的丙型肝炎病毒的方法，包括将待测试的病毒接种到用鼠逆转录病毒感染的细胞，培养细胞，并将病毒分配给具有高度感染性的丙型肝炎病毒 如果病毒在细胞中复制，或者如果病毒不在细胞中复制，则将病毒分配给具有较少感染性的丙型肝炎病毒的感染性。