公开(公告)号：US08679859B2

公开(公告)日：2014-03-25

申请号：US12455269

申请日：2009-05-28

申请人： Mingdi Yan ,  Olof Ramström ,  Li-Hong Liu ,  Xin Wang ,  Michael M. Lerner ,  Tosapol Maluangnont

发明人： Mingdi Yan ,  Olof Ramström ,  Li-Hong Liu ,  Xin Wang ,  Michael M. Lerner ,  Tosapol Maluangnont

IPC分类号： G01N33/543

CPC分类号： B82Y25/00 ,  C01B32/19 ,  H01F1/0054

摘要： Devices comprising functionalized materials, and embodiments of a method for making and using such devices, are disclosed. Exemplary devices include ophthalmic devices, nanoparticles, quartz crystal microbalances, microarrays, and nanocomposites. In particular embodiments, device surfaces are modified with monomers and/or polymers, typically carbohydrate monomers and/or polymers. Embodiments of a method for making and using such devices are disclosed. Monomers and/or polymers are covalently bonded to surfaces using functionalized perhalophenylazides. In some embodiments, device surfaces are functionalized with a perhalophenylazide. One or more monomers and/or polymers subsequently are covalently bonded to the device surface using the perhalophenylazide. In other embodiments, monomers and/or polymers are derivatized with a functionalized perhalophenylazide. The derivatized monomers and/or polymers then are covalently bonded to the device surface using the perhalophenylazide. In some embodiments, graphite is functionalized with perhalophenylazide to produce a functionalized graphene sheet. In other embodiments, graphene sheets are covalently attached to functionalized substrates.

摘要翻译： 公开了包括功能化材料的装置以及用于制造和使用这些装置的方法的实施例。 示例性装置包括眼科装置，纳米颗粒，石英晶体微量平衡，微阵列和纳米复合材料。 在具体实施方案中，装置表面用单体和/或聚合物（通常为碳水化合物单体和/或聚合物）进行改性。 公开了制造和使用这种装置的方法的实施例。 使用官能化的全卤代叠氮基，单体和/或聚合物共价键合到表面上。 在一些实施方案中，装置表面用全卤代苯偶酰胺官能化。 随后使用全卤代苯偶酰共价键将一种或多种单体和/或聚合物共价键合到器件表面。 在其它实施方案中，单体和/或聚合物用官能化的全卤代叠氮化物衍生化。 然后，衍生化的单体和/或聚合物使用全卤代苯偶酰共价键合到器件表面。 在一些实施方案中，石墨用全卤代苯偶氮官能化以产生官能化的石墨烯片。 在其它实施方案中，石墨烯片共价连接到官能化基材上。

公开(公告)号：US5830539A

公开(公告)日：1998-11-03

申请号：US565199

申请日：1995-11-17

申请人： Mingdi Yan ,  John F. W. Keana ,  Goran Karapetrov ,  Christopher J-P Sevrain ,  Martin N. Wybourne

发明人： Mingdi Yan ,  John F. W. Keana ,  Goran Karapetrov ,  Christopher J-P Sevrain ,  Martin N. Wybourne

IPC分类号： A61L27/28 ,  A61L33/00 ,  B05D1/18 ,  B05D7/14 ,  B05D3/06 ,  B05D3/10

CPC分类号： B82Y30/00 ,  A61L27/28 ,  A61L33/0076 ,  B05D1/185 ,  B82Y40/00 ,  B01J2219/00497 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00659

摘要： Methods for coating substrates are described. The methods comprise coating at least a portion of a substrate with particular coating materials. The coating materials can be crosslinked and coated onto a substrate. Alternatively, the coating materials may be covalently bonded to the substrates. The coating materials might themselves functionalize the substrate, or provide a biocompatible coating on the substrate. The coating materials might also include electrophilic or nucleophilic groups that allow for the subsequent reaction of the coating materials with additional reagents. The present invention also provides coated workpieces, particularly medical workpieces having a surface for contacting tissue or blood. These workpieces comprise a first layer and a second layer. The first layer comprises a molecular tether covalently bonded to the surface. The second layer is bonded to the article by the first layer and comprises a bioactive agent selected from the group consisting of biocompatible polymers, antibiotics, antimicrobials and anticoagulants.

摘要翻译： 描述了涂覆基材的方法。 所述方法包括用特定涂层材料涂覆至少一部分基底。 涂层材料可以交联并涂覆在基材上。 或者，涂层材料可以共价键合到基底上。 涂层材料本身可以使基底功能化，或在基材上提供生物相容的涂层。 涂层材料还可以包括允许涂层材料随后与另外的试剂反应的亲电或亲核基团。 本发明还提供涂覆的工件，特别是具有用于接触组织或血液的表面的医疗工件。 这些工件包括第一层和第二层。 第一层包括共价结合到表面上的分子系链。 第二层通过第一层结合到制品上，并且包含选自生物相容性聚合物，抗生素，抗微生物剂和抗凝剂的生物活性剂。

公开(公告)号：US5582955A

公开(公告)日：1996-12-10

申请号：US495005

申请日：1995-06-26

申请人： John F. W. Keana ,  Martin N. Wybourne ,  Sui X. Cai ,  Mingdi Yan

发明人： John F. W. Keana ,  Martin N. Wybourne ,  Sui X. Cai ,  Mingdi Yan

IPC分类号： C08F8/30 ,  C08F8/34 ,  G03C5/00

CPC分类号： C08F8/30 ,  C08F8/34 ,  Y10S430/143

摘要： Methods for covalently modifying surfaces of various substrates are disclosed, along with various substrates having surfaces modified by such methods. Candidate surfaces include various polymeric, siliceous, metallic, allotrophic forms of carbon, and semiconductor surfaces. The surfaces are exposed to a reagent, having molecules each comprising a nitrenogenic group and a functionalizing group, in the presence of energized charged particles such as electrons and ions, photons, or heat, which transform the nitrenogenic reagent to a nitrene intermediate. The nitrene covalently reacts with any of various chemical groups present on the substrate surface, thereby effecting nitrene addition of the functionalizing groups to the substrate surface. The functionalizing groups can then participate in downstream chemistry whereby any of a large variety of functional groups, including biological molecules, can be covalently bonded to the surface, thereby dramatically altering the chemical behavior of the surface. Such functionalizations of the surface can be done in a single reactive step or in multiple reactive steps.

公开(公告)号：US5580697A

公开(公告)日：1996-12-03

申请号：US267851

申请日：1994-06-23

申请人： John F. W. Keana ,  Martin N. Wybourne ,  Sui X. Cai ,  Mingdi Yan

发明人： John F. W. Keana ,  Martin N. Wybourne ,  Sui X. Cai ,  Mingdi Yan

IPC分类号： C08J7/12 ,  B01J19/08 ,  C01B31/04 ,  C08F8/34 ,  C23C16/02 ,  G01N21/45 ,  G01N21/77 ,  G01N33/543 ,  G03F7/20 ,  H01L21/368 ,  G03C5/00

CPC分类号： G01N33/54373 ,  C08F8/32 ,  G01N21/7703 ,  G03F7/2041 ,  G01N2201/0873 ,  Y10S430/143

摘要： Methods for covalently modifying surfaces of various substrates are disclosed, along with various substrates having surfaces modified by such methods. Candidate surfaces include various polymeric, siliceous, metallic, allotrophic forms of carbon, and semiconductor surfaces. The surfaces are exposed to a reagent, having molecules each comprising a nitrenogenic group and a functionalizing group, in the presence of energized charged particles such as electrons and ions, photons, or heat, which transform the nitrenogenic reagent to a nitrene intermediate. The nitrene covalently reacts with any of various chemical groups present on the substrate surface, thereby effecting nitrene addition of the functionalizing groups to the substrate surface. The functionalizing groups can then participate in downstream chemistry whereby any of a large variety of functional groups, including biological molecules, can be covalently bonded to the surface, thereby dramatically altering the chemical behavior of the surface. Such functionalizations of the surface can be done in a single reactive step or in multiple reactive steps.

公开(公告)号：US20100028559A1

公开(公告)日：2010-02-04

申请号：US12455269

申请日：2009-05-28

申请人： Mingdi Yan ,  Olof Ramström ,  Li-Hong Liu ,  Xin Wang ,  Michael M. Lerner ,  Tosapol Maluangnont

发明人： Mingdi Yan ,  Olof Ramström ,  Li-Hong Liu ,  Xin Wang ,  Michael M. Lerner ,  Tosapol Maluangnont

IPC分类号： B05D3/06 ,  C08B37/00 ,  C08F120/18 ,  C07K14/00 ,  B05D3/02 ,  C07K16/00

CPC分类号： B82Y25/00 ,  C01B32/19 ,  H01F1/0054

摘要： Devices comprising functionalized materials, and embodiments of a method for making and using such devices, are disclosed. Exemplary devices include ophthalmic devices, nanoparticles, quartz crystal microbalances, microarrays, and nanocomposites. In particular embodiments, device surfaces are modified with monomers and/or polymers, typically carbohydrate monomers and/or polymers. Embodiments of a method for making and using such devices are disclosed. Monomers and/or polymers are covalently bonded to surfaces using functionalized perhalophenylazides. In some embodiments, device surfaces are functionalized with a perhalophenylazide. One or more monomers and/or polymers subsequently are covalently bonded to the device surface using the perhalophenylazide. In other embodiments, monomers and/or polymers are derivatized with a functionalized perhalophenylazide. The derivatized monomers and/or polymers then are covalently bonded to the device surface using the perhalophenylazide. In some embodiments, graphite is functionalized with perhalophenylazide to produce a functionalized graphene sheet. In other embodiments, graphene sheets are covalently attached to functionalized substrates.

摘要翻译： 公开了包括功能化材料的装置以及用于制造和使用这些装置的方法的实施例。 示例性装置包括眼科装置，纳米颗粒，石英晶体微量平衡，微阵列和纳米复合材料。 在具体实施方案中，装置表面用单体和/或聚合物（通常为碳水化合物单体和/或聚合物）进行改性。 公开了制造和使用这种装置的方法的实施例。 使用官能化的全卤代叠氮基，单体和/或聚合物共价键合到表面上。 在一些实施方案中，装置表面用全卤代苯偶酰胺官能化。 随后使用全卤代苯偶酰共价键将一种或多种单体和/或聚合物共价结合到装置表面。 在其它实施方案中，单体和/或聚合物用官能化的全卤代叠氮化物衍生化。 然后，衍生化的单体和/或聚合物使用全卤代苯偶酰共价键合到器件表面。 在一些实施方案中，石墨用全卤代苯偶氮官能化以产生官能化的石墨烯片。 在其它实施方案中，石墨烯片共价连接到官能化基材上。

公开(公告)号：US5465151A

公开(公告)日：1995-11-07

申请号：US14425

申请日：1993-02-05

申请人： Martin N. Wybourne ,  John F. W. Keana ,  Sui X. Cai ,  Mingdi Yan ,  Jong Wu

发明人： Martin N. Wybourne ,  John F. W. Keana ,  Sui X. Cai ,  Mingdi Yan ,  Jong Wu

IPC分类号： C08J7/12 ,  B01J19/08 ,  C01B31/04 ,  C08F8/34 ,  C23C16/02 ,  G01N21/27 ,  G01N21/45 ,  G01N21/64 ,  G01N21/75 ,  G01N21/77 ,  G01N33/543 ,  G01N33/566 ,  G03F7/20 ,  H01L21/368 ,  G01B9/02

CPC分类号： G01N21/7703 ,  C08F8/32 ,  G01N33/54373 ,  G03F7/2041 ,  G01N2021/7779 ,  G01N2201/0873

摘要： Chemical and biosensors are disclosed. An optical waveguide is used to conduct electromagnetic radiation by total internal reflection in parallel through a reference waveguide portion and at least one analyte waveguide portion. The electromagnetic radiation is then converged into an exit beam. The external surface of at least the analyte portion is covalently modified, or functionalized, relative to the reference portion. Resulting interaction of the functionalized surface with molecules comprising an analyte causes a phase change in the electromagnetic radiation passing through the analyte portion relative to the reference portion sufficient to generate a corresponding and measurable interference pattern in the exit beam. A waveguide surface is functionalized by exposure to a reagent, having molecules each comprising a nitrenogenic group and a functionalizing group, in the presence of energized charged particles such as electrons and ions, photons, or heat, which transform the nitrenogenic reagent to a nitrene intermediate. The resulting reaction causes the functionalizing groups to covalently bond to the surface. The functionalizing groups can then participate in downstream chemistry whereby any of a large variety of functional groups, including biological molecules, can be covalently bonded to the surface. Thus, the waveguide surface can be made selectively responsive to a wide variety of analytes, including cells and other biological structures.

摘要翻译： 公开了化学和生物传感器。 光波导用于通过参考波导部分和至少一个分析物波导部分并行地通过全内反射来传导电磁辐射。 电磁辐射然后会聚到出射光束中。 至少分析物部分的外表面相对于参考部分共价修饰或官能化。 功能化表面与包含分析物的分子的相互作用导致相对于参考部分通过分析物部分的电磁辐射的相变足以在出射光束中产生相应和可测量的干涉图案。 波导表面通过暴露于具有各自包含硝基衍生基团和官能化基团的分子的试剂而被功能化，所述分子在有电的带电粒子例如电子和离子，光子或热的情况下，其将硝化反应试剂转变为硝酸中间体 。 所得到的反应使官能团共价键合到表面。 官能团然后可以参与下游化学，因此包括生物分子在内的各种各样的官能团中的任何一个可以共价键合到表面上。 因此，波导表面可以选择性地响应于各种各样的分析物，包括细胞和其他生物结构。

公开(公告)号：US20130252843A1

公开(公告)日：2013-09-26

申请号：US13988495

申请日：2011-11-22

申请人： Mingdi Yan ,  Xin Wang ,  H. S. N. Jayawardena

发明人： Mingdi Yan ,  Xin Wang ,  H. S. N. Jayawardena

IPC分类号： G01N33/58

CPC分类号： G01N33/587 ,  A61K49/0043 ,  A61K49/0052 ,  A61K49/0054 ,  A61K49/0065 ,  A61K49/0093 ,  G01N2400/00 ,  Y10T436/143333

摘要： Disclosed embodiments concern differentiating and classifying one or more targets using a perhalophenylazide-derived nanoparticle probe, or multiple such probes. Particular embodiments concern using statistical analysis to produce score plots illustrating the level of differentiation and/or classification. Also disclosed are methods for making perhalophenylazide-derived nanoparticle probes, individually or by using a microarray technique. Particular embodiments concern methods for using the per halophenylazide-derived nanoparticle probes to diagnose, detect, and/or treat a disease. Kits comprising the perhalophenylazide-derived nanoparticle probes are also disclosed.

摘要翻译： 公开的实施方案涉及使用全卤代苯偶氮衍生的纳米颗粒探针或多种这样的探针来分化和分类一种或多种靶。 特定实施例涉及使用统计分析来产生评估图，其显示分化水平和/或分类。 还公开了单独或通过使用微阵列技术制备全卤代苯偶氮衍生的纳米颗粒探针的方法。 具体实施方案涉及使用每卤代苯偶氮衍生的纳米颗粒探针来诊断，检测和/或治疗疾病的方法。 还公开了包含全卤代苯偶氮衍生的纳米颗粒探针的试剂盒。

公开(公告)号：US20080214410A1

公开(公告)日：2008-09-04

申请号：US11775190

申请日：2007-07-09

申请人： Mingdi Yan ,  Li Liu ,  John Gann

发明人： Mingdi Yan ,  Li Liu ,  John Gann

IPC分类号： C40B40/14 ,  C40B50/16

CPC分类号： C40B40/14 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00497 ,  B01J2219/00511 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/00711 ,  B01J2219/00716 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00731 ,  B01J2219/00736 ,  B82Y30/00

摘要： Embodiments of a method for covalently immobilizing one or more discrete molecules on a substrate and embodiments of substrates having covalently-immobilized discrete molecules are disclosed. Embodiments of the method can include exposing a substrate to a functionalizing reagent to form a functionalized substrate and exposing the functionalized substrate to a solution comprising the molecule to be immobilized. A reaction-energy source then can be used to activate the functionalizing reagent and covalently bond one or more of the molecules to the substrate. All or a substantial portion of the unbonded molecules then can be removed. Controlling the concentration of the functionalizing reagent to which the substrate is exposed allows the density of the bonding sites on the substrate to be reduced so that, after removal of the unbonded molecules, at least one of the bonded molecules remains on the substrate spatially isolated from any other bonded molecules.

摘要翻译： 公开了一种用于将一个或多个离散分子共价固定在基底上的方法和具有共价固定的离散分子的基底的实施方案的实施方案。 该方法的实施方案可以包括将底物暴露于官能化试剂以形成功能化底物，并将官能化底物暴露于包含待固定分子的溶液中。 然后可以使用反应能源来活化官能化试剂并将一个或多个分子共价键合到基底上。 然后可以除去全部或大部分未结合的分子。 控制底物暴露于其上的官能化试剂的浓度可使底物上的键合位点的密度降低，使得在除去未结合的分子后，至少一个键合分子保留在基质上空间上与 任何其他键合分子。

公开(公告)号：US07405034B2

公开(公告)日：2008-07-29

申请号：US10769423

申请日：2004-01-30

申请人： Mingdi Yan ,  Michele A. Bartlett

发明人： Mingdi Yan ,  Michele A. Bartlett

IPC分类号： G03F7/00 ,  B05D3/00

CPC分类号： C40B60/14 ,  B01J2219/00432 ,  B01J2219/00443 ,  B01J2219/00531 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B82Y30/00 ,  C40B40/10 ,  G03F7/0035 ,  G03F7/008 ,  G03F7/012 ,  G03F7/11

摘要： Methods of adhering polymeric materials to a substrate, either directly or through linker molecules, are disclosed. Structures, for example, microstructures, including microwells and arrays of microwells, may be readily formed using the methods. In some embodiments, microstructures formed completely from polymeric materials are provided, making it possible to tailor the chemical and physical properties of the microstructures. For example, microwells having a bottom comprising a polar polymeric material and well sides/top comprising a non-polar polymeric material are provided. Biochemical reagents may be easily delivered to such “smart wells” because the intrinsic attraction of the well bottom for the reagents and the intrinsic repulsion between the well sides/top combine to direct the reagents to the wells.

摘要翻译： 公开了将聚合物材料直接或通过连接分子粘附到基底上的方法。 可以使用这些方法容易地形成结构，例如微结构，包括微孔和微孔阵列。 在一些实施方案中，提供了由聚合材料完全形成的微结构，使得可以调整微结构的化学和物理性能。 例如，提供具有包含极性聚合物材料的底部的微孔和包含非极性聚合物材料的阱侧/顶部。 生物化学试剂可以很容易地传递到这种“智能井”，因为井底对于试剂的内在吸引力和阱侧/顶部之间的固有排斥结合起来将试剂引导到井。

公开(公告)号：US6022597A

公开(公告)日：2000-02-08

申请号：US966058

申请日：1997-11-07

申请人： Mingdi Yan ,  John F. W. Keana

发明人： Mingdi Yan ,  John F. W. Keana

IPC分类号： C08J5/06 ,  G03F7/008 ,  G03F7/16 ,  B05D3/06

CPC分类号： C08J5/06 ,  G03F7/008 ,  G03F7/165 ,  C08J2321/00

摘要： Methods for covalently modifying surfaces of various substrates are disclosed, along with various substrates having surfaces modified by such methods. Candidate surfaces include various polymeric, siliceous, metallic, allotrophic forms of carbon, and semiconductor surfaces. The surfaces are exposed to a reagent, having molecules each comprising a nitrenogenic group and a functionalizing group, in the presence of energized charged particles such as electrons and ions, photons, or heat, which transform the nitrenogenic reagent to a nitrene intermediate. The nitrene covalently reacts with any of various chemical groups present on the substrate surface, thereby effecting nitrene addition of the functionalizing groups to the substrate surface. The functionalizing groups can then participate in downstream chemistry whereby any of a large variety of functional groups, including biological molecules, can be covalently bonded to the surface, thereby dramatically altering the chemical behavior of the surface. Such functionalizations of the surface can be done in a single reactive step or in multiple reactive steps.

摘要翻译： 公开了共价修饰各种基材的表面的方法，以及具有通过这些方法修饰的表面的各种基材。 候选表面包括各种聚合物，硅质，金属，同位素形式的碳和半导体表面。 表面暴露于试剂，其具有各自包含硝基衍生基团和官能化基团的分子，在激发带电粒子例如电子和离子，光子或热的存在下，其将硝化试剂转化为硝酸中间体。 所述氮烯与存在于所述基材表面上的各种化学基团共价反应，由此将所述官能团添加到所述基材表面上。 官能化基团然后可以参与下游化学，由此可以将大量各种官能团（包括生物分子）中的任何一个共价键合到表面，从而显着地改变表面的化学行为。 表面的这种功能化可以在单个反应步骤中或在多个反应步骤中进行。