公开(公告)号：US20130196877A1

公开(公告)日：2013-08-01

申请号：US13877346

申请日：2011-10-04

申请人： Nilanjana Banerjee ,  Angel Janevski ,  Sitharthan Kamalakaran ,  Vinay Varadan ,  Nevenka Dimitrova ,  Robert Lucito

发明人： Nilanjana Banerjee ,  Angel Janevski ,  Sitharthan Kamalakaran ,  Vinay Varadan ,  Nevenka Dimitrova ,  Robert Lucito

IPC分类号： G06F19/20

CPC分类号： G16B25/00 ,  G16B5/00 ,  G16B20/00

摘要： The present invention relates to a method for identifying multi-modal associations between biomedical markers which allows for the determination of network nodes and/or high ranking network members or combinations thereof, indicative of having a diagnostic, prognostic or predictive value for a medical condition, in particular ovarian cancer. The present invention further relates to a biomedical marker or group of biomedical markers associated with a high likelihood of responsiveness of a subject to a cancer therapy, preferably a platinum based cancer therapy, wherein said bio-medical marker or group of biomedical markers comprises at least 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 8, 19, 20 or all markers selected from PKMYT1, SKIL, RAB8A, HIRIP3, CTNNB1, NGFR, ZCCHC11, LSP1, CD200, PAX8, CYBRD1, HOXC11, TCEAL1, FZD10,FZD1, BBS4, IRS2, TLX3, TSPAN2, TXN, and CFLAR. Furthermore, an assay for detecting, diagnosing, graduating, monitoring or prognosticating a medical condition, or for detecting, 1 diagnosing, monitoring or prognosticating the responsiveness of a subject to a therapy against said medical condition, in particular ovarian cancer, is provided, as well as a corresponding method for classifying a subject comprising and a medical decision support system.

摘要翻译： 本发明涉及一种用于识别生物医学标记之间的多模式关联的方法，其允许确定网络节点和/或高排名网络成员或其组合，指示具有用于医疗状况的诊断，预后或预测值， 特别是卵巢癌。 本发明还涉及与受试者对癌症治疗（优选基于铂的癌症治疗）的高反应性的高可能性相关联的生物医学标记物或生物医学标记物组，其中所述生物医学标记物或生物医学标记物组至少包括 1,2,3,4,5,6,7,8,9,10,11,12,13,14,15,16,17,8,19,20或选自PKMYT1，SKIL，RAB8A， HIRIP3，CTNNB1，NGFR，ZCCHC11，LSP1，CD200，PAX8，CYBRD1，HOXC11，TCEAL1，FZD10，FZD1，BBS4，IRS2，TLX3，TSPAN2，TXN和CFLAR。 此外，提供了用于检测，诊断，毕业，监测或预测医学状况或用于检测1诊断，监测或预测受试者对于针对所述医学病症，特别是卵巢癌的治疗的反应性的测定法，作为 以及用于分类受试者的相应方法以及医疗决策支持系统。

公开(公告)号：US08357112B2

公开(公告)日：2013-01-22

申请号：US12530243

申请日：2008-03-03

申请人： Nevenka Dimitrova ,  Christopher S. Hall ,  Chien T. Chin

发明人： Nevenka Dimitrova ,  Christopher S. Hall ,  Chien T. Chin

IPC分类号： A61B17/20

CPC分类号： A61B8/481 ,  A61K9/0009 ,  A61K41/0028 ,  A61K47/6925 ,  A61M37/0092

摘要： This invention relates to a method and an apparatus of controlling a release of a material carried by ultrasound sensitive particles, the release being caused by irradiating the ultrasound sensitive particles with an ultrasonic pulse having acoustic properties selected so as to interact with the ultrasound sensitive particles and thus causing the release of the material. The ultrasound sensitive particles comprise sub-groups of ultrasound sensitive particles, the ultrasound sensitive particles within the same sub-group having their respective acoustic property causing each respective sub-group to interact independently with the sound wave.

摘要翻译： 本发明涉及一种控制由超声敏感颗粒携带的材料的释放的方法和装置，该释放是通过用选择的声学性质的超声脉冲照射超声波敏感颗粒而引起的，以便与超声敏感颗粒相互作用， 从而导致材料的释放。 超声敏感颗粒包括超声敏感颗粒的子组，相同子组内的超声敏感颗粒具有各自的声学特性，导致每个相应的子组与声波独立地相互作用。

公开(公告)号：US08199991B2

公开(公告)日：2012-06-12

申请号：US12516931

申请日：2007-12-03

申请人： Lalitha Agnihotri ,  James David Schaffer ,  Nevenka Dimitrova

发明人： Lalitha Agnihotri ,  James David Schaffer ,  Nevenka Dimitrova

IPC分类号： G06K9/36

CPC分类号： G06K9/00134 ,  G06K9/3233 ,  G06K9/3275 ,  G06T7/70 ,  G06T2207/20056 ,  G06T2207/30072

摘要： A method of automatically identifying the microarray chip corners and probes, even if there are no probes at the corners, in a high density and high resolution microarray scanned image having an image space, wherein the method minimizes the error distortions in the image arising in the scanning process by applying to the image a multipass corner finding algorithm comprising: (a) applying a Radon transform to an input microarray image to project the image into an angle and distance space where it is possible to find the orientation of the straight lines; (b) applying a fast Fourier transform to the projected image of (a) to find the optimal tilting angle of the projected image; (c) determining the optimal first and last local maxima for the optimal tilting angle; (d) back projecting the determined first and last local maxima to the image space to find the first approximation of the first and last column lines of the image; (e) rotating the image and repeating steps (a) through (d) to find the first approximation of the top and bottom row lines of the image; (f) determining the first approximation of the four corners of the image from the intersection of the column and row lines; (g) applying a heuristic for determining if the first approximation of step (f) is sufficient; and (h) optionally trimming the scanned image around the first approximation of the four corners and repeating steps (a) through (f).

摘要翻译： 即使在具有图像空间的高密度和高分辨率的微阵列扫描图像中，即使在角落处没有探针也能够自动识别微阵列芯片角部和探针的方法，其中该方法使图像中产生的图像中的误差失真最小化 扫描过程，通过向图像应用多点角发现算法，包括：（a）将Radon变换应用于输入微阵列图像以将图像投影到可以找到直线的取向的角度和距离空间中; （b）对（a）的投影图像应用快速傅立叶变换以找到投影图像的最佳倾斜角; （c）确定最佳倾斜角的最佳第一和最后局部最大值; （d）将确定的第一和最后局部最大值向前投影到图像空间，以找到图像的第一列和最后一列的第一近似; （e）旋转图像并重复步骤（a）至（d）以找到图像的顶行和下行行的第一近似值; （f）从列和行之间的交点确定图像的四个角的第一近似值; （g）应用启发式来确定步骤（f）的第一近似是否足够; 和（h）可选地修整围绕四个角的第一近似的扫描图像并重复步骤（a）至（f）。

公开(公告)号：US20120053071A1

公开(公告)日：2012-03-01

申请号：US13140972

申请日：2009-12-14

申请人： Nevenka Dimitrova ,  Chetan Mittal ,  Sitharthan Kamalakaran

发明人： Nevenka Dimitrova ,  Chetan Mittal ,  Sitharthan Kamalakaran

IPC分类号： C40B30/04 ,  C40B30/00 ,  G06F19/00 ,  C12Q1/68

CPC分类号： C12Q1/6827 ,  C12Q2523/125

摘要： The present invention relates to a method for the detection of a DNA methylation signature associated with the presence of or the predisposition to develop a disorder, the method comprising the identification of one or more candidate genes exhibiting differential DNA methylation in target and reference samples as well as the respective determination of the nucleic acid sites in said candidate genes that are differentially methylated and the recognition sites for DNA binding factors, said DNA binding factors each recognizing such a differentially methylated nucleic acid site, wherein the patterns of differentially methylated nucleic acid sites and of DNA binding factor recognition sites obtained together represent a DNA methylation signature that is indicative for the presence of or the predisposition to develop a disorder in a target sample.

摘要翻译： 本发明涉及一种用于检测与发生病症的存在或倾向相关的DNA甲基化特征的方法，所述方法包括鉴定靶和参照样品中显示差异DNA甲基化的一个或多个候选基因 作为差异甲基化的所述候选基因中的核酸位点和DNA结合因子的识别位点的各自测定，所述DNA结合因子各自识别这样的差异甲基化核酸位点，其中差异甲基化的核酸位点和 一起获得的DNA结合因子识别位点代表一种DNA甲基化特征，其指示在目标样品中存在发生障碍的倾向或倾向。

公开(公告)号：US20110189098A1

公开(公告)日：2011-08-04

申请号：US12993109

申请日：2009-05-14

申请人： Nevenka Dimitrova ,  Chetan Mital

发明人： Nevenka Dimitrova ,  Chetan Mital

IPC分类号： A61K49/00 ,  C12Q1/68 ,  C07H21/00

CPC分类号： C12N15/62 ,  C07K14/43595 ,  C07K14/71 ,  C07K2319/60 ,  C12Q1/6897 ,  C12Q2537/164 ,  C12Q2525/207 ,  C12Q2525/143

摘要： The present invention relates to a method of non-invasively monitoring the expression of a gene of interest in a cell when contacting said cell with a compound influencing the expression of said gene of interest. The present invention is also concerned with different isolated nucleic acid molecules comprising a coding sequence. Said coding sequence comprises a gene of interest-sequence encoding a gene of interest-polypeptide fused to a reporter sequence encoding a fluorescent reporter polypeptide and is operatively coupled to a promoter sequence. The present invention is also concerned with the use of a method and a nucleic acid molecule of the invention for delivering a compound influencing the expression of a gene of interest in a cell, monitoring the delivery of said compound as well as monitoring the influence on the expression of said gene fluorescence of interest induced by said compound at the same time.

摘要翻译： 本发明涉及当使所述细胞与影响目标基因表达的化合物接触时非侵入性地监测细胞中目的基因的表达的方法。 本发明还涉及包含编码序列的不同分离的核酸分子。 所述编码序列包含编​​码与编码荧光报道多肽的报告基因融合的感兴趣基因多肽的目标基因序列，并且可操作地偶联至启动子序列。 本发明还涉及本发明的方法和核酸分子用于递送影响细胞中目的基因表达的化合物，监测所述化合物的递送以及监测对所述化合物的影响 所述化合物同时诱导的所述感兴趣的基因荧光的表达。

公开(公告)号：US20100239502A1

公开(公告)日：2010-09-23

申请号：US12668551

申请日：2008-06-26

申请人： Evan Edward Santo ,  Nevenka Dimitrova ,  CHIEN Ting Chien

发明人： Evan Edward Santo ,  Nevenka Dimitrova ,  CHIEN Ting Chien

IPC分类号： A61K49/00 ,  C12N15/85 ,  C12Q1/68

CPC分类号： C12N15/1086 ,  C12Q1/6897 ,  C12Q2565/101

摘要： The invention features compositions and methods for in vivo expression analysis. The data presented herein demonstrates that ultrasound-enhanced delivery and/or expression of a composition for expression analysis comprising microbubbles vectors as well as a genetic payload, comprising a “always-on” promoter, a “reference” reporter gene, a “query” promoter and an “answer” reporter gene, enables in vivo analysis of gene expression both without requiring prior preparation (especially genetic modification) of the test subject (animal or patient) and without causing long term or systemic effects on the subject. Such an invention can be used, for example, to query the epigenotypic or phenotypic response of the individual subject to a foreign effector substance such as a pyrogen, pharmaceutical compound, pharmaceutical lead compound, an allergen, an autoimmunogene, a toxin, a polyclonal antibody, a monoclonal antibody, an antigen, a lipid, a carbohydrate, a peptide, a protein, a protein-complex, an amino acid, a fatty acid, a nucleotide, DNA, RNA, PNA, siRNA and micro RNA.

摘要翻译： 本发明的特征在于用于体内表达分析的组合物和方法。 本文提供的数据表明包括微泡载体和遗传有效载荷的用于表达分析的组合物的超声增强递送和/或表达，包括“始终在上”启动子，“参考”报告基因，“查询” 启动子和“答案”报告基因，能够在不需要测试对象（动物或患者）的事先准备（特别是遗传修饰）的情况下进行基因表达的体内分析，并且不会对受试者造成长期或全身影响。 这样的发明可以用于例如查询个体受试者对外源性效应物质如热原，药物化合物，药物引导化合物，变应原，自身免疫原，毒素，多克隆抗体的表型表型或表型反应 ，单克隆抗体，抗原，脂质，碳水化合物，肽，蛋白质，蛋白质复合物，氨基酸，脂肪酸，核苷酸，DNA，RNA，PNA，siRNA和微RNA。

公开(公告)号：US20090129647A1

公开(公告)日：2009-05-21

申请号：US12282435

申请日：2007-03-07

申请人： Nevenka Dimitrova ,  Yee Him Cheung

发明人： Nevenka Dimitrova ,  Yee Him Cheung

IPC分类号： G06K9/00

CPC分类号： G06F19/22 ,  G06F19/24 ,  G06F19/26

摘要： Spectrogram extraction from DNA sequence has been known since 2001. A DNA spectrogram is generated by applying Fourier transform to convert a symbolic DNA sequence consisting of letters A, T, C, G into a visual representation that highlights periodicities of co-occurrence of DNA patterns. Given a DNA sequence or whole genomes, with this method it is easy to generate a large number of spectrogram images. However, the difficult part is to elucidate where are the repetitive patterns and to associate a biological and clinical meaning to them. The present disclosure provides systems and methods that facilitate the location and/or identification of repetitive DNA patterns, such as CpG islands, Alu repeats, tandem repeats and various types of satellite repeats. These repetitive elements can be found within a chromosome, within a genome or across genomes of various species. The disclosed systems and methods apply image processing operators to find prominent features in the vertical and horizontal direction of the DNA spectrograms. Systems and methods for fast, full scale analysis of the derived images using supervised machine learning methods are also disclosed. The disclosed systems and methods for detecting and/or classifying repetitive DNA patterns include: (a) comparative histogram method, (b) feature selection and classification using support vector machines and genetic algorithms, and (c) generation of spectrovideo from a plurality of spectral images.

摘要翻译： 从2001年开始，DNA序列的谱图提取是已知的。通过应用傅立叶变换将由字母A，T，C，G组成的符号DNA序列转换成突出显示DNA图案共同出现周期的视觉表示，生成DNA谱图 。 给定一个DNA序列或整个基因组，用这种方法很容易产生大量的谱图。 然而，困难的部分是阐明重复模式在哪里，并将生物和临床意义与他们联系起来。 本公开提供了促进重复DNA模式（例如CpG岛，Alu重复序列，串联重复序列和各种类型的卫星重复）的定位和/或识别的系统和方法。 这些重复元件可以在染色体内，基因组内或各种物种的基因组中发现。 所公开的系统和方法应用图像处理算子以在DNA谱图的垂直和水平方向上发现显着特征。 还公开了使用监督机器学习方法对衍生图像进行快速，全面分析的系统和方法。 用于检测和/或分类重复DNA模式的所公开的系统和方法包括：（a）比较直方图方法，（b）使用支持向量机和遗传算法的特征选择和分类，以及（c）从多个光谱 图片。

公开(公告)号：US07457811B2

公开(公告)日：2008-11-25

申请号：US10178015

申请日：2002-06-21

申请人： Angel Janevski ,  Nevenka Dimitrova ,  Lalitha Agnihotri

发明人： Angel Janevski ,  Nevenka Dimitrova ,  Lalitha Agnihotri

IPC分类号： G06F17/00

CPC分类号： G06F17/30828 ,  G06F17/30867 ,  H04N21/44218 ,  H04N21/44222 ,  Y10S707/99942 ,  Y10S707/99943 ,  Y10S707/99944 ,  Y10S707/99945

摘要： A content maintenance system uses a time-dependent precipitation function for iteratively augmenting or removing content over time, after an initial demonstration of user interest. A plurality of parallel precipitation processes can be launched simultaneously in response to different facets of a user expression of interest. Precipitation is dependent on highlighting or extracting segment descriptors from content of interest to the user. Then segments are filtered, rated, annotated and/or prioritized from that content. The remaining segments are matched against stored search structures. When the segments match, they are precipitated out for storage and can generate new search structures.

摘要翻译： 在用户感兴趣的初步演示之后，内容维护系统使用时间依赖的降水函数来迭代地增加或移除内容。 响应于感兴趣的用户表达的不同方面，可以同时启动多个并行降水过程。 降水取决于从用户感兴趣的内容中突出显示或提取片段描述符。 然后，细分受到过滤，评分，注释和/或从内容中排列。 剩余的段与存储的搜索结构匹配。 当段匹配时，它们被沉淀出来用于存储，并且可以生成新的搜索结构。

公开(公告)号：US07356830B1

公开(公告)日：2008-04-08

申请号：US09351086

申请日：1999-07-09

申请人： Nevenka Dimitrova

发明人： Nevenka Dimitrova

IPC分类号： H04N5/455 ,  H04N7/173

CPC分类号： G06K9/00758 ,  G06F17/30787 ,  G06F17/30796 ,  G06F17/30799 ,  G06F17/30855 ,  H04H20/93 ,  H04N7/24 ,  H04N21/858

摘要： A given video segment is configured to include links to one or more other video segments or information sources. The given video segment is processed in a video processing system to determine an association between an object, entity, characterization or other feature of the segment and at least one additional information source containing the same feature. The association is then utilized to access information from the additional information source, such that the accessed information can be displayed to a user in conjunction with or in place of the original video segment. A set of associations for the video segment can be stored in a database or other memory of the processing system, or incorporated into the video segment itself, e.g., in a transport stream of the video segment. The additional information source may be, e.g., an additional video segment which includes the designated feature, or a source of audio, text or other information containing the designated feature. The feature may be a video feature extracted from a frame of the video segment, e.g., an identification of a particular face, scene, event or object in the frame, an audio feature such as a music signature extraction, a speaker identification, or a transcript extraction, or a textual feature. The invention allows a user to access information by clicking on or otherwise selecting an object or other feature in a displayed video segment, thereby facilitating the retrieval of information related to that segment.

摘要翻译： 给定的视频段被配置为包括到一个或多个其他视频段或信息源的链接。 给定视频段在视频处理系统中被处理以确定该段的对象，实体，表征或其他特征与包含相同特征的至少一个附加信息源之间的关联。 然后，该关联用于从附加信息源访问信息，使得所访问的信息可以结合或替代原始视频段来显示给用户。 用于视频段的一组关联可以存储在处理系统的数据库或其他存储器中，或者例如在视频段的传输流中被并入视频段本身。 附加信息源可以是例如包括指定特征的附加视频段，或包含指定特征的音频，文本或其它信息的来源。 该特征可以是从视频段的帧中提取的视频特征，例如，帧中的特定面部，场景，事件或对象的识别，诸如音乐签名提取，扬声器标识或音频特征的音频特征 转录本提取或文本功能。 本发明允许用户通过点击或以其它方式选择所显示的视频段中的对象或其他特征来访问信息，从而便于检索与该段有关的信息。

公开(公告)号：US07337455B2

公开(公告)日：2008-02-26

申请号：US10029916

申请日：2001-12-31

申请人： Lalitha Agnihotri ,  J. David Schaffer ,  Nevenka Dimitrova ,  Thomas McGee ,  Sylvie Jeannin

发明人： Lalitha Agnihotri ,  J. David Schaffer ,  Nevenka Dimitrova ,  Thomas McGee ,  Sylvie Jeannin

IPC分类号： H04N7/10 ,  H04N7/025

CPC分类号： G06K9/00718 ,  G06F17/30787 ,  G06F17/30802 ,  G06K9/62 ,  G11B27/28 ,  G11B2220/20 ,  H04N5/76 ,  H04N21/4147 ,  H04N21/44008 ,  H04N21/812

摘要： A method for optimizing the performance of an algorithm for detecting predetermined content in a media information stream, and a program and apparatus that operate in accordance with the method. The algorithm is a function of a set of parameters. The method comprises the steps of performing the algorithm at least once to detect the predetermined content in the media information stream, while employing a respective set of parameters in the algorithm for each performance thereof, and automatically evolving at least one respective set of parameters employed in the algorithm to maximize the degree of accuracy at which the algorithm detects the predetermined content in the media information stream.

摘要翻译： 一种用于优化用于检测媒体信息流中的预定内容的算法的性能的方法，以及根据该方法操作的程序和装置。 该算法是一组参数的函数。 该方法包括以下步骤：至少一次执行该算法以检测媒体信息流中的预定内容，同时在算法中对其每次演奏采用相应的一组参数，并且自动演化至少一个相应的参数集 最大化算法在媒体信息流中检测预定内容的准确度的算法。