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    Competitive immunoassay method, device and test kit
    4.
    发明授权
    Competitive immunoassay method, device and test kit 失效
    竞争性免疫分析方法，装置和检测试剂盒

    公开(公告)号：US4803170A

    公开(公告)日：1989-02-07

    申请号：US946475

    申请日：1986-12-24

    申请人： Thomas H. Stanton ,  James A. Clagett ,  Deborah C. Schindele ,  George E. Renzoni ,  Niels H. Andersen ,  Kent E. Opheim

    发明人： Thomas H. Stanton ,  James A. Clagett ,  Deborah C. Schindele ,  George E. Renzoni ,  Niels H. Andersen ,  Kent E. Opheim

    IPC分类号： G01N33/533 ,  G01N33/543 ,  G01N33/558 ,  G01N33/53

    CPC分类号： G01N33/54306 ,  G01N33/533 ,  G01N33/558 ,  Y10S436/807 ,  Y10S436/808 ,  Y10S436/809

    摘要： An immunoassay device including one or more reaction chambers, each adapted to receive and retain a volume of test fluid in fluid communication with nonoverlapping first, second, and third reagent-bearing surfaces. To the first surface is reversibly bound an analyte conjugate: analyte component conjugated to one or more components, termed ligand/marker, that serve ligand and marker functions as described herein. Analyte binding partner is immobilized on the second surface, and ligand/marker binding partner is immobilized on the third surface. The reaction chamber is preferably configured to receive and direct the test fluid sequentially past the first second, and third reagent surfaces. In use, analyte conjugate solubilized from the first surface completes with any analyte in the test fluid for analyte binding partner sites on the second surface. Excess analyte conjugate becomes sequestered on the third surface, where the marker activity is read to indicate analyte presence and concentration in the test fluid. A test kit includes the immunoassay device in combination with comparative test results.

    摘要翻译： 一种包括一个或多个反应室的免疫测定装置，每个反应室适于容纳和保持与非重叠的第一，第二和第三试剂载体表面流体连通的一定体积的测试流体。 第一表面可逆地结合分析物缀合物：与一种或多种组分缀合的分析物成分，称为配体/标记，其用于如本文所述的配体和标记功能。 分析物结合配偶体固定在第二表面上，并且配体/标记结合配偶体固定在第三表面上。 反应室优选地构造成接收并引导测试流体依次经过第一和第三试剂表面。 在使用中，从第一表面溶解的分析物结合物与第二表面上分析物结合配偶位点的测试流体中的任何分析物完成。 超量分析物缀合物在第三表面上被隔离，其中读取标记活性以指示分析物在测试流体中的存在和浓度。 测试试剂盒包括免疫测定装置和比较试验结果。