-
1.发明授权公开(公告)号：US07138254B2
公开(公告)日：2006-11-21
申请号：US10361481
申请日：2003-02-07
CPC分类号： C12Q1/6806 , B01J2219/00286 , B01J2219/00369 , B01J2219/00511 , B01J2219/00585 , B01J2219/00691 , B01J2219/00702 , B01J2219/00722 , B01L3/0241 , B01L3/5025 , B01L7/52 , B01L2300/0838 , B01L2300/1844 , B01L2400/0409 , C12Q1/6869 , C40B40/06 , C40B60/14 , C12Q2565/518 , C12Q2535/101 , C12Q2527/125
摘要： Methods for preparing nanoscale reactions using nucleic acids or proteins are presented. Nucleic acids are captured saturably, yet reversibly, on the internal surface of the reaction chamber, typically a capillary. Excess nucleic acid is removed and the reaction is performed directly within the capillary. Proteins are captured specifically and saturably on the modified inner surface of the reaction chamber, typically a capillary. Excess protein is removed and the reaction is performed directly within the capillary. Devices for effecting the methods of the invention and a system designed advantageously to utilize the methods for high throughput reactions involving nucleic acids or proteins are also provided.
-
公开(公告)号：US08557518B2
公开(公告)日：2013-10-15
申请号：US12845650
申请日：2010-07-28
申请人： Stevan Bogdan Jovanovich , Iuliu I. Blaga , Michael Nguyen , William D. Nielsen , Mattias Vangbo
发明人： Stevan Bogdan Jovanovich , Iuliu I. Blaga , Michael Nguyen , William D. Nielsen , Mattias Vangbo
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/028 , B01L2200/0668 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2300/088 , B01L2300/0887 , B01L2300/0896 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B82Y30/00 , F16K99/0001
摘要： The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.
摘要翻译： 本发明公开了可编程微流体电路的集成，以实现处理生化和化学反应的实际应用并整合这些反应。 在一些实施方案中，组合用于生化反应或化学工作流程的工作流程。 公开了诸如具有Y阀和流过阀的可编程微流体电路的微型阀。 在一些实施方案中，本发明的微型阀用于混合流体，其可以是整合过程的一部分。 这些过程包括将样品和移动反应混合到用于模块化微流体的边缘或储存器，使用捕获区域以及将其注入到分离设备上的分析装置中。 在一些实施例中，使用星形和嵌套星形设计，或者通过微通道中的通道的横截面积的改变来进行珠捕获。 使用固定温度和通过微型泵将样品移动进一步公开了温度区之间样品的移动。
-
公开(公告)号：US08551714B2
公开(公告)日：2013-10-08
申请号：US13367326
申请日：2012-02-06
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0059 , B01J2219/005 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/028 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2200/12 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/1822 , B01L2300/1844 , B01L2400/0409 , B01L2400/0421 , B01L2400/043 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , C12Q2565/629 , G01N1/34 , G01N27/44721 , G01N27/44743 , G01N27/44791 , G01N35/0098
摘要： Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
摘要翻译： 公开了用于将微芯片连接到各种类型的模块的方法和装置。 所公开的技术可用作各种应用的样品制备和分析系统，如DNA测序和基因分型，蛋白质组学，病原体检测，诊断和生物防治。
-
公开(公告)号：US20120088249A1
公开(公告)日：2012-04-12
申请号：US13287398
申请日：2011-11-02
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0059 , B01J2219/005 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/028 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2200/12 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/1822 , B01L2300/1844 , B01L2400/0409 , B01L2400/0421 , B01L2400/043 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , C12Q2565/629 , G01N1/34 , G01N27/44721 , G01N27/44743 , G01N27/44791 , G01N35/0098
摘要： Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
摘要翻译： 公开了用于将微芯片连接到各种类型的模块的方法和装置。 所公开的技术可用作各种应用的样品制备和分析系统，如DNA测序和基因分型，蛋白质组学，病原体检测，诊断和生物防治。
-
公开(公告)号：US08431390B2
公开(公告)日：2013-04-30
申请号：US13287398
申请日：2011-11-02
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0059 , B01J2219/005 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/028 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2200/12 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/1822 , B01L2300/1844 , B01L2400/0409 , B01L2400/0421 , B01L2400/043 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , C12Q2565/629 , G01N1/34 , G01N27/44721 , G01N27/44743 , G01N27/44791 , G01N35/0098
摘要： Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
摘要翻译： 公开了用于将微芯片连接到各种类型的模块的方法和装置。 所公开的技术可用作各种应用的样品制备和分析系统，如DNA测序和基因分型，蛋白质组学，病原体检测，诊断和生物防治。
-
公开(公告)号：US20110039303A1
公开(公告)日：2011-02-17
申请号：US12526015
申请日：2008-02-05
申请人： Stevan Bogdan Jovanovich , Iuliu Ioan Blaga , Allen Boronkay , Joanne Horn , Michael Van Nguyen , William D. Nielsen , Mattias Vangbo
发明人： Stevan Bogdan Jovanovich , Iuliu Ioan Blaga , Allen Boronkay , Joanne Horn , Michael Van Nguyen , William D. Nielsen , Mattias Vangbo
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/028 , B01L2200/0668 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2300/088 , B01L2300/0887 , B01L2300/0896 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B82Y30/00 , F16K99/0001
摘要： The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.
摘要翻译： 本发明公开了可编程微流体电路的集成，以实现处理生化和化学反应的实际应用并整合这些反应。 在一些实施方案中，组合用于生化反应或化学工作流程的工作流程。 公开了诸如具有Y阀和流过阀的可编程微流体电路的微型阀。 在一些实施方案中，本发明的微型阀用于混合流体，其可以是整合过程的一部分。 这些过程包括将样品和移动反应混合到用于模块化微流体的边缘或储存器，使用捕获区域以及将其注入到分离设备上的分析装置中。 在一些实施例中，使用星形和嵌套星形设计，或者通过微通道中的通道的横截面积的改变来进行珠捕获。 使用固定温度和通过微型泵将样品移动进一步公开了温度区之间样品的移动。
-
公开(公告)号：US09121058B2
公开(公告)日：2015-09-01
申请号：US13202884
申请日：2011-08-20
CPC分类号： B01J19/0046 , B01J2219/00389 , B01J2219/00599 , B01J2219/00716 , B01J2219/00722 , B01L3/502738 , B01L7/52 , B01L2200/0647 , B01L2200/10 , B01L2200/16 , B01L2300/0816 , B01L2300/0867 , B01L2300/087 , B01L2400/043 , B01L2400/0481 , B01L2400/0655 , C12Q1/6806 , C12Q1/6869 , C12Q1/6874 , F16K99/0015 , F16K99/0059 , F16K2099/0084
摘要： The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.
摘要翻译： 本发明提供用于执行综合分析的系统，设备，方法和套件。 综合分析可以包括样品处理，库构建，扩增和测序。 集成分析可以在一个或多个流体连接的模块中进行。 一个或多个模块可以由计算机控制和/或自动化。 可以对组织样品，临床样品或环境样品进行综合分析。 集成分析系统可以具有紧凑的格式，并在指定的时间段内返回结果。
-
公开(公告)号：US20100068723A1
公开(公告)日：2010-03-18
申请号：US12605217
申请日：2009-10-23
IPC分类号： C12Q1/68 , G01N33/543 , G01N1/14
CPC分类号： B01L3/50273 , B01F11/0071 , B01F13/0059 , B01J2219/005 , B01L3/502715 , B01L3/502738 , B01L3/502761 , B01L7/52 , B01L9/527 , B01L2200/027 , B01L2200/028 , B01L2200/0647 , B01L2200/0668 , B01L2200/10 , B01L2200/12 , B01L2300/0654 , B01L2300/0803 , B01L2300/0806 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/087 , B01L2300/0874 , B01L2300/0887 , B01L2300/1822 , B01L2300/1844 , B01L2400/0409 , B01L2400/0421 , B01L2400/043 , B01L2400/0487 , B01L2400/0638 , B01L2400/0655 , B01L2400/086 , C12Q2565/629 , G01N1/34 , G01N27/44721 , G01N27/44743 , G01N27/44791 , G01N35/0098
摘要： Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such asDNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense.
摘要翻译： 公开了用于将微芯片连接到各种类型的模块的方法和装置。 所公开的技术可用作各种应用的样品制备和分析系统，如DNA测序和基因分型，蛋白质组学，病原体检测，诊断和生物防治。
-
公开(公告)号：US06870185B2
公开(公告)日：2005-03-22
申请号：US10633171
申请日：2003-08-01
CPC分类号： B01L3/502738 , B01L3/502715 , B01L2200/027 , B01L2200/10 , B01L2300/0861 , B01L2400/0487 , B01L2400/0622 , B01L2400/0644 , B01L2400/065 , G01N27/44743 , G01N27/44791
摘要： Structures and methods that facilitate integration and/or isolation of various functions in a microchip system are disclosed. In one embodiment, the integration of the functions is by a multi-chip, sliding linear valve approach. The chips are in continued physical contact. In a second embodiment, the chips are separated and rejoined when they are moved to the preferred position. Surface coating of the joining edges helps prevent leakage and keeps liquid in the capillary channels for both embodiments. Another embodiment relates to miniature valves. Several designs were disclosed, including the linear, edge-contact sliding valve approach. Method to fabricate very small, high aspect ratio holes in glass was also disclosed, which facilitates the above embodiments.
-
公开(公告)号：US20140045704A1
公开(公告)日：2014-02-13
申请号：US13886068
申请日：2013-05-02
申请人： Stevan Bogdan Jovanovich , luliu I. Balga , Allen R. Boronkay , Joanne Horn , Michael Van Nguyen , William D. Nielsen , Mattias Vangbo
发明人： Stevan Bogdan Jovanovich , luliu I. Balga , Allen R. Boronkay , Joanne Horn , Michael Van Nguyen , William D. Nielsen , Mattias Vangbo
CPC分类号： C12Q1/686 , B01L3/502715 , B01L3/50273 , B01L3/502761 , B01L7/52 , B01L7/525 , B01L2200/028 , B01L2200/0668 , B01L2200/10 , B01L2300/0816 , B01L2300/0867 , B01L2300/0874 , B01L2300/088 , B01L2300/0887 , B01L2300/0896 , B01L2300/1827 , B01L2400/0481 , B01L2400/0655 , B33Y80/00 , B82Y30/00 , F16K99/0001
摘要： The present invention discloses the integration of programmable microfluidic circuits to achieve practical applications to process biochemical and chemical reactions and to integrate these reactions. In some embodiments workflows for biochemical reactions or chemical workflows are combined. Microvalves such as programmable microfluidic circuit with Y valves and flow through valves are disclosed. In some embodiments microvalves of the present invention are used for mixing fluids, which may be part of an integrated process. These processes include mixing samples and moving reactions to an edge or reservoir for modular microfluidics, use of capture regions, and injection into analytical devices on separate devices. In some embodiments star and nested star designs, or bead capture by change of cross sectional area of a channel in a microvalve are used. Movement of samples between temperature zones are further disclosed using fixed temperature and movement of the samples by micropumps.
摘要翻译： 本发明公开了可编程微流体电路的集成，以实现处理生化和化学反应的实际应用并整合这些反应。 在一些实施方案中，组合用于生化反应或化学工作流程的工作流程。 公开了诸如具有Y阀和流过阀的可编程微流体电路的微型阀。 在一些实施方案中，本发明的微型阀用于混合流体，其可以是整合过程的一部分。 这些过程包括将样品和移动反应混合到用于模块化微流体的边缘或储存器，使用捕获区域以及将其注入分离装置中的分析装置中。 在一些实施例中，使用星形和嵌套星形设计，或者通过微通道中的通道的横截面积的改变来进行珠捕获。 使用固定温度和通过微型泵将样品移动进一步公开了温度区之间样品的移动。
-
-
-
-
-
-
-
-
-
搜索结果
17 条记录 (耗时 0.028 秒)
