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公开(公告)号：US07625701B2
公开(公告)日：2009-12-01
申请号：US11154419
申请日：2005-06-15
申请人： John G. K. Williams , Gregory Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi Reynolds , Pamela Sheaff
发明人： John G. K. Williams , Gregory Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi Reynolds , Pamela Sheaff
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charged-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US08148516B2
公开(公告)日：2012-04-03
申请号：US12854137
申请日：2010-08-10
申请人： John G. K. Williams , Gregory R Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
发明人： John G. K. Williams , Gregory R Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US07659070B2
公开(公告)日：2010-02-09
申请号：US11925722
申请日：2007-10-26
申请人： John G. K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
发明人： John G. K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US20110039266A1
公开(公告)日：2011-02-17
申请号：US12854137
申请日：2010-08-10
申请人： John G.K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
发明人： John G.K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
IPC分类号： C12Q1/68 , C07H19/048 , C07K9/00
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US20080153095A1
公开(公告)日：2008-06-26
申请号：US11925722
申请日：2007-10-26
申请人： John G.K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
发明人： John G.K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
IPC分类号： C12Q1/68
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US20080206764A1
公开(公告)日：2008-08-28
申请号：US11925724
申请日：2007-10-26
申请人： John G.K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
发明人： John G.K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US20060063173A1
公开(公告)日：2006-03-23
申请号：US11154419
申请日：2005-06-15
申请人： John Williams , Gregory Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi Reynolds , Pamela Sheaff
发明人： John Williams , Gregory Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi Reynolds , Pamela Sheaff
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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公开(公告)号：US06936702B2
公开(公告)日：2005-08-30
申请号：US09876374
申请日：2001-06-06
申请人： John G. K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
发明人： John G. K. Williams , Gregory R. Bashford , Jiyan Chen , Dan Draney , Nara Narayanan , Bambi L. Reynolds , Pamela Sheaff
IPC分类号： G01N21/64 , C07B61/00 , C07H19/10 , C07H19/20 , C07H21/00 , C12N15/09 , C12Q1/00 , C12Q1/42 , C12Q1/44 , C12Q1/48 , C12Q1/68 , G01N27/447 , G01N33/483 , G01N33/53 , C07H19/00 , C07H21/02 , C07H21/04
CPC分类号： C07H19/10 , C07H19/20 , C07H21/00 , C12Q1/6869 , C40B40/00 , C12Q2565/629 , C12Q2565/607 , C12Q2565/301 , C12Q2533/101
摘要： The present invention provides compounds, methods and systems for sequencing nucleic acid using single molecule detection. Using labeled NPs that exhibit charge-switching behavior, single-molecule DNA sequencing in a microchannel sorting system is realized. In operation, sequencing products are detected enabling real-time sequencing as successive detectable moieties flow through a detection channel. By electrically sorting charged molecules, the cleaved product molecules are detected in isolation without interference from unincorporated NPs and without illuminating the polymerase-DNA complex.
摘要翻译： 本发明提供了使用单分子检测来测序核酸的化合物，方法和系统。 使用表现出电荷转换行为的标记NP，实现了微通道分选系统中的单分子DNA测序。 在操作中，随着连续的可检测部分流经检测通道，测序产物被检测成能够进行实时测序。 通过电分选带电分子，分离检测产物分子而不受未结合的NP的干扰，而不照射聚合酶-DNA复合物。
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