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    Recovery of rare cells using a microchannel apparatus with patterned posts
    2.
    发明授权
    Recovery of rare cells using a microchannel apparatus with patterned posts 有权
    使用具有图案柱的微通道装置回收稀有电池

    公开(公告)号：US08158410B2

    公开(公告)日：2012-04-17

    申请号：US11038920

    申请日：2005-01-18

    申请人： Zhongliang Tang ,  Ram S. Bhatt ,  Pavel Tsinberg

    发明人： Zhongliang Tang ,  Ram S. Bhatt ,  Pavel Tsinberg

    IPC分类号： C12M1/34 ,  C12M3/00

    CPC分类号： G01N1/40 ,  G01N1/405 ,  Y10T436/25375

    摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.

    摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流线，其中直线流被横向柱的图案中断。 柱在流动路径中的收集区域的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有直线表面，具有弧形横截面，并且被随机排列以便破坏流线型流动。 螯合剂如Abs通过亲水涂层，优选含有非常长的异氰酸酯部分或PEG或聚甘氨酸的水凝胶附着在收集区域中的所有表面上，因此在捕获细胞或其它靶向生物分子中是非常有效的 这种流线型流动中断。

    DEVICES AND METHODS OF CELL CAPTURE AND ANALYSIS
    3.
    发明申请
    DEVICES AND METHODS OF CELL CAPTURE AND ANALYSIS 有权
    细胞捕获和分析的设备和方法

    公开(公告)号：US20100255479A1

    公开(公告)日：2010-10-07

    申请号：US12730738

    申请日：2010-03-24

    申请人： Stephen Mikolajczyk ,  Tony Pircher ,  Pavel Tsinberg ,  Farideh Z. Bischoff

    发明人： Stephen Mikolajczyk ,  Tony Pircher ,  Pavel Tsinberg ,  Farideh Z. Bischoff

    IPC分类号： G01N33/574 ,  G01N33/53 ,  C12M1/34 ,  C12Q1/68

    CPC分类号： G01N33/54306 ,  G01N33/536 ,  G01N33/56966 ,  G01N33/57492

    摘要： The present invention provides a device for isolating target biomolecules or cells from samples, particularly biological samples. In particular, the device comprises a loading mixture, which contains the biological sample and a first binding entity that specifically binds to the target biomolecule or target cell; and a micro-channel coated with a second binding entity that binds directly or indirectly to the first binding entity. Methods of capturing, detecting, and/or evaluating target biomolecules or target cells (e.g. cancer cells) in biological samples are also disclosed.

    摘要翻译： 本发明提供了用于从样品，特别是生物样品中分离靶生物分子或细胞的装置。 具体地说，该装置包括载体混合物，其含有生物样品和特异性结合靶生物分子或靶细胞的第一结合实体; 以及涂覆有与第一结合实体直接或间接结合的第二结合实体的微通道。 还公开了在生物样品中捕获，检测和/或评估靶生物分子或靶细胞（例如癌细胞）的方法。

    CELL SEPARATION USING MICROCHANNEL HAVING PATTERNED POSTS
    4.
    发明申请
    CELL SEPARATION USING MICROCHANNEL HAVING PATTERNED POSTS 有权
    使用具有图案位置的微通道的细胞分离

    公开(公告)号：US20130121895A1

    公开(公告)日：2013-05-16

    申请号：US13525225

    申请日：2012-06-15

    申请人： Zhongliang TANG ,  Pavel TSINBERG ,  Ram S. BHATT

    发明人： Zhongliang TANG ,  Pavel TSINBERG ,  Ram S. BHATT

    IPC分类号： G01N1/34

    CPC分类号： G01N1/34 ,  B01F5/102 ,  B01F13/0059 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/088 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0655 ,  B01L2400/086 ,  G01N1/40 ,  G01N1/405 ,  G01N33/54366

    摘要： A micro flow device (11, 71) for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts (23, 81). The posts are spaced across the width of an expanded collection chamber region (17, 75) in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, being curved in cross-sections, e.g. circular or tear-drop shaped, and are randomly arranged so as to disrupt streamlined flow. The device is oriented so that its lower surface is aligned at about 45° to the horizontal. Sequestering agents, such as Abs, which are attached to surfaces of the collection region via a hydrophilic coating, preferably a permeable hydrogel containing isocyanate moieties, are highly effective in capturing cells or other targeted biomolecules while the remainder of the liquid sample exits horizontally.

    摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流量装置（11,71）使用流线，其中直线流被横向柱（23,81）的图案中断。 所述柱在所述流动路径中的扩张的收集室区域（17,75）的宽度上间隔开，并在其上表面和下表面之间延伸; 它们具有弯曲的横截面的直线表面，例如。 圆形或泪滴形，并且随机布置以便破坏流线型流。 该装置被定向成使得其下表面与水平面成约45°对准。 通过亲水涂层，优选含有异氰酸酯部分的渗透性水凝胶连接到收集区域的表面上的螯合剂如Abs在捕获细胞或其它靶向生物分子中而其余液体样品水平离开时是非常有效的。

    Device for cell separation and analysis and method of using
    6.
    发明授权
    Device for cell separation and analysis and method of using 有权
    用于细胞分离和分析的装置及其使用方法

    公开(公告)号：US07695956B2

    公开(公告)日：2010-04-13

    申请号：US11331988

    申请日：2006-01-12

    申请人： Pavel Tsinberg ,  Zhongliang Tang

    发明人： Pavel Tsinberg ,  Zhongliang Tang

    IPC分类号： C12M3/00

    CPC分类号： G01N1/40 ,  B01L3/5027 ,  G01N1/2813

    摘要： A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.

    摘要翻译： 用于从体液或其他液体样品中分离或分离细胞的微流装置使用流动路径，其中直线流被横向柱的图案中断，所述横向柱横跨收集区域的宽度以不规则或设置的随机图案 以便破坏流线型流程。 螯合剂如Abs通过亲水可渗透的水凝胶涂层附着在收集区域中的所有表面上。 收集区域形成为由PDMS成型的主体中的空腔，该柔性体被夹在玻璃滑块或类似的平板和刚性顶盖板之间，两者都通过热量与PDMS体压接成邻接关系 -shrunk聚合物套管。 细胞分离和洗涤后，细胞可以从多价螯合剂中释放出来，离心的装置迫使细胞收集在水凝胶涂层的载玻片或板附近。 分开聚合物套筒可以使用整体制表片将身体从滑块或板上剥离，以暴露其顶部表面上的分离的细胞，以进行显微镜检查。

    Microarrays utilizing hydrogels
    7.
    发明授权
    Microarrays utilizing hydrogels 有权
    利用水凝胶的微阵列

    公开(公告)号：US07595157B2

    公开(公告)日：2009-09-29

    申请号：US10922391

    申请日：2004-08-19

    申请人： Pavel Tsinberg

    发明人： Pavel Tsinberg

    IPC分类号： C12Q1/68

    CPC分类号： G01N33/54353 ,  B01J19/0046 ,  B01J2219/00387 ,  B01J2219/00527 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00621 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  G01N33/54393 ,  Y10S524/916

    摘要： A method of making a microarray by coating a flat substrate with a polymerizable hydrogel layer which contains anchoring moieties dispersed uniformly therethroughout. Following curing, a continuous layer of uniform thickness is securely attached to the upper surface of the substrate through an array region thereof. A plurality of different probes are then attached to create microspots at distinct spatial locations on the surface of this slab layer by linking the probes to the anchoring moieties in the cured hydrogel. Such anchoring moieties may employ linking systems such as organic chelators, that are activated by copper or some other metal, and complementary pairs such as avidin-biotin.

    摘要翻译： 通过用可聚合的水凝胶层涂覆平坦的基底来制备微阵列的方法，该可聚合水凝胶层包含在整个中均匀分散的锚定部分。 在固化之后，通过其阵列区域将均匀厚度的连续层牢固地附着到基板的上表面。 然后连接多个不同的探针以通过将探针连接到固化的水凝胶中的锚定部分而在该板层的表面上的不同空间位置产生微孔。 这种锚定部分可以使用由铜或一些其它金属活化的连接系统，例如有机螯合剂，以及互补对，例如抗生物素蛋白 - 生物素。

    Alleviation of non-specific binding in microarray assays
    8.
    发明授权
    Alleviation of non-specific binding in microarray assays 失效
    微阵列测定中非特异性结合的缓解

    公开(公告)号：US07258990B2

    公开(公告)日：2007-08-21

    申请号：US10922387

    申请日：2004-08-19

    申请人： Yehudit Falcovitz-Gerassi ,  Pavel Tsinberg

    发明人： Yehudit Falcovitz-Gerassi ,  Pavel Tsinberg

    IPC分类号： G01N33/53

    CPC分类号： G01N33/54393 ,  G01N33/543

    摘要： A post-incubation treatment is employed to effectively remove targets, such as proteins/protein complexes, or other label-bearing moieties that may non-specifically bind to a microarray substrate during a binding assay. Following incubation, a one-step wash is carried out with a liquid containing digester, e.g., a digestive enzyme (protease) or lysosome, which is effective to remove non-specifically bound targets or at least labeled portions of such targets from the substrate. Proteases are bound to or coated onto large molecules or onto solid particles of such a size such that they are prohibited from entering the porous surfaces of 3-D hydrogel microspots and are unable to reach and digest labeled target-probe complexes that are disposed within such porous hydrogel microspots. Digested segments of such protein which contain labels (or of essentially the entire protein) are carried away in the wash liquid and thus are not present to create background noise during imaging.

    摘要翻译： 采用后孵育处理以在结合测定期间有效地去除可能非特异性结合微阵列底物的靶蛋白质/蛋白质复合物或其它标记物部分。 孵育后，用含有液体的消化器（例如消化酶（蛋白酶）或溶酶体）进行一步洗涤，其有效地从底物除去非特异性结合的靶标或至少标记的这些靶标部分。 蛋白酶结合或涂覆在大分子上或者如此大小的固体颗粒上，使得它们被禁止进入3-D水凝胶微孔的多孔表面，并且不能到达并消化被置于其内的标记的靶 - 探针复合物 多孔水凝胶微孢子。 含有标签（或基本上整个蛋白质）的蛋白质的消化片段在洗涤液体中被携带，因此不存在以在成像期间产生背景噪声。

    Recovery of rare cells using a microchannel apparatus with patterned posts
    10.
    发明授权
    Recovery of rare cells using a microchannel apparatus with patterned posts 有权

    公开(公告)号：US08076127B2

    公开(公告)日：2011-12-13

    申请号：US11038920

    申请日：2005-01-18

    申请人： Zhongliang Tang ,  Ram S. Bhatt ,  Pavel Tsinberg

    发明人： Zhongliang Tang ,  Ram S. Bhatt ,  Pavel Tsinberg

    IPC分类号： C12M1/34 ,  C12M3/00

    摘要： A microflow apparatus for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts. The posts are spaced across the width of a collection region in the flow path, extending between the upper and lower surfaces thereof; they have rectilinear surfaces, have arcuate cross-sections, and are randomly arranged so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic coating, preferably a hydrogel containing isocyanate moieties or a PEG or polyglycine of substantial length, and are highly effective in capturing cells or other targeted biomolecules as a result of such streamlined flow disruption.