公开(公告)号：US20150079694A2

公开(公告)日：2015-03-19

申请号：US13811959

申请日：2011-07-19

申请人： Roland SCHENK

发明人： Roland SCHENK

IPC分类号： B01L3/00

CPC分类号： B01L3/508 ,  B01F5/0647 ,  B01F13/0059 ,  B01F13/1016 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0487 ,  G01N21/05 ,  G01N21/648 ,  G01N2021/0346 ,  G01N2021/056

摘要： An interchangeable disposable measuring cassette for insertion into a measuring apparatus for detecting target molecules in a liquid sample by measuring fluorescence emission has a flow measurement cell in which an excitation radiation provided by the measuring apparatus produces an evanescent field in the liquid sample beyond a boundary layer for the liquid sample and the measurement cell. To be better able to ensure that no sample liquid can cross from the measurement cell into the measuring apparatus, the measuring cassette includes a body including an optically transparent material and a base in contact with the underside of the body. The measurement cell is formed by a cutout provided in the body, the base, or both. The areas on which the body and the base are on top of one another around this cutout are connected to one another directly and in fluid-tight fashion by laser welding.

摘要翻译： 用于插入到用于通过测量荧光发射来检测液体样品中的目标分子的测量装置的可互换的一次性测量盒具有流量测量单元，其中由测量装置提供的激发辐射在液体样品中产生超出边界层的渐逝场 用于液体样品和测量池。 为了更好地确保没有样品液体可以从测量单元穿过测量装置，测量盒包括主体，其包括光学透明材料和与主体下侧接触的基部。 测量单元由设置在主体，基座或两者中的切口形成。 主体和基座在该切口周围彼此顶部的区域通过激光焊接直接并以流体密封的方式彼此连接。

公开(公告)号：US5126241A

公开(公告)日：1992-06-30

申请号：US418516

申请日：1989-10-10

申请人： Roland Schenk

发明人： Roland Schenk

IPC分类号： G01N33/543 ,  G01N33/78

CPC分类号： G01N33/78 ,  G01N33/54306

摘要： The present invention provides a process for the determination of a specifically bindable substance by incubation of a sample solution with at least three receptors R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 is specifically bindable with R.sub.2 and R.sub.3, as well as with the substance to be determined, R.sub.2 brings about the binding to a solid phase and R.sub.3 carries a labelling, separation of bound labelling from unbound labelling and measurement of the labelling in one of the two phases, wherein, as receptor R.sub.1, a receptor is used which has at least two binding positions which bind specifically with an epitope of the substance to be determined, as R.sub.2 a conjugate of a partner P.sub.1 of a specifically binding pair and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, the partner P.sub.1 thereby either being bound to a solid phase or being immobilized, and as R.sub.3 a complex is used which contains at least the substance S and a labelling.

摘要翻译： 本发明提供了一种通过将样品溶液与至少三种R 1，R 2和R 3（其中R 1与R 2和R 3特异性结合）的受体R 1，R 2和R 3以及与 确定，R2引起与固相的结合，R3携带标记，将结合的标记与未结合标记的分离以及在两个相之一中的标记的测量，其中作为受体R1，使用至少具有至少 作为R2与特异性结合对的配偶体P1的结合物和对应于待测物质或其衍生物的物质S的结合物特异性结合待测物质的表位的两个结合位置， 要测定的物质的至少一个表位，配偶体P1因此被结合到固相或固定化，并且作为R3，使用至少含有物质的复合物 e S和标签。

公开(公告)号：US09517466B2

公开(公告)日：2016-12-13

申请号：US13811959

申请日：2011-07-19

申请人： Roland Schenk

发明人： Roland Schenk

IPC分类号： G01N21/00 ,  G01N21/75 ,  G01N21/03 ,  B01L3/00

CPC分类号： B01L3/508 ,  B01F5/0647 ,  B01F13/0059 ,  B01F13/1016 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0487 ,  G01N21/05 ,  G01N21/648 ,  G01N2021/0346 ,  G01N2021/056

摘要： An interchangeable disposable measuring cassette for insertion into a measuring apparatus for detecting target molecules in a liquid sample by measuring fluorescence emission has a flow measurement cell in which an excitation radiation provided by the measuring apparatus produces an evanescent field in the liquid sample beyond a boundary layer for the liquid sample and the measurement cell. To be better able to ensure that no sample liquid can cross from the measurement cell into the measuring apparatus, the measuring cassette includes a body including an optically transparent material and a base in contact with the underside of the body. The measurement cell is formed by a cutout provided in the body, the base, or both. The areas on which the body and the base are on top of one another around this cutout are connected to one another directly and in fluid-tight fashion by laser welding.

摘要翻译： 用于插入到用于通过测量荧光发射来检测液体样品中的目标分子的测量装置的可互换的一次性测量盒具有流量测量单元，其中由测量装置提供的激发辐射在液体样品中产生超出边界层的渐逝场 用于液体样品和测量池。 为了更好地确保没有样品液体可以从测量单元穿过测量装置，测量盒包括主体，其包括光学透明材料和与主体下侧接触的基部。 测量单元由设置在主体，基座或两者中的切口形成。 主体和基座在该切口周围彼此顶部的区域通过激光焊接直接并以流体密封的方式彼此连接。

公开(公告)号：US20110232397A1

公开(公告)日：2011-09-29

申请号：US13129627

申请日：2009-11-16

申请人： Roland SCHENK

发明人： Roland SCHENK

IPC分类号： B01L3/02

CPC分类号： B01L3/022 ,  B01L2200/0621 ,  G01N35/10 ,  G01N35/1004 ,  G01N35/1016

摘要： The invention relates to an automatic pipetting device, which comprises at least two pumping units of different capacities and a liquid line, said liquid line opening on one side into a rinsing liquid reservoir and on the other side into a pipetting needle. In order for one pumping unit to be able to execute a pipetting cycle with small volumes at high stroke resolution and the other pumping unit to be able to execute a rinse cycle with large volumes, and to make the pumping units as space-saving, as low-maintenance and as inexpensive as possible, it is proposed according to the invention to drive the pistons of the at least two pumping units synchronously by a piston drive device and to arrange the point at which the largest working chamber is connected to the liquid line closer to the rinsing liquid reservoir than the point at which the working chamber of the at least one other pumping unit is connected to the liquid line, wherein moreover a 3-way valve is arranged on the liquid line at the point at which the largest working chamber is connected to the liquid line.

摘要翻译： 本发明涉及一种自动移液装置，其包括至少两个不同容量的泵送单元和液体管线，所述液体管线一侧开口到冲洗液体储存器中，另一侧开口进入移液针头。 为了使一个泵送单元能够以高冲程分辨率执行小体积的移液循环，另一个泵送单元能够执行大体积的冲洗循环，并且使得泵送单元节省空间，如 低维护性并且尽可能廉价，根据本发明提出，通过活塞驱动装置同步地驱动至少两个泵送单元的活塞，并且将最大工作室连接到液体管线的点 比所述至少一个其它泵送单元的工作室连接到所述液体管线的点更靠近所述冲洗液体储存器，此外，在所述液体管线上布置有最大工作点 室连接到液体管线。

公开(公告)号：US5858803A

公开(公告)日：1999-01-12

申请号：US376504

申请日：1989-07-05

申请人： Roland Schenk ,  Dietmar Zdunek

发明人： Roland Schenk ,  Dietmar Zdunek

IPC分类号： G01N33/536 ,  G01N33/53 ,  G01N33/538 ,  G01N33/542 ,  G01N33/543

CPC分类号： G01N33/538 ,  G01N33/53 ,  G01N33/54313

摘要： The present invention provides a process for the determination of a specifically bindable substance by incubation of the sample solution with at least three receptors R.sub.1, R.sub.2 and R.sub.3, of which R.sub.1 and R.sub.2 are bindable with one another and R.sub.3 is specifically bindable with the substance to be determined and measurement of the agglutination which takes place in the case of the reaction, wherein, as receptor R.sub.1, there is used a conjugate of a partner of a specifically binding pair P and of a substance S which corresponds to the substance to be determined or is a derivative thereof and has at least one epitope of the substance to be determined, as receptor R.sub.2 there is used a receptor which has at least two binding positions for P and as receptor R.sub.3 there is used a receptor which has at least two binding positions, of which at least one binds specifically with an epitope of the substance to be determined or of S. The present invention also provides a reagent for carrying out the above process.

摘要翻译： 本发明提供了通过将样品溶液与至少三种受体R1，R2和R3（其中R1和R2可彼此结合）和R3与该物质特异性结合的受体R1，R2和R3的温育来测定特异性可结合物质的方法， 确定和测量在反应情况下发生的凝集，其中作为受体R1，使用特异性结合对P的配偶体和对应于待测物质的物质S的缀合物 或者是其衍生物，并且具有待测定物质的至少一个表位，因为受体R2使用对P具有至少两个结合位置的受体，并且作为受体R3使用具有至少两个结合的受体 位置，其至少一个与待测物质的表位或S特异性结合。本发明还提供了用于进行上述方法的试剂。

公开(公告)号：US5437981A

公开(公告)日：1995-08-01

申请号：US887587

申请日：1992-05-20

申请人： Arno Deger ,  Roland Schenk ,  Gerhard Bienhaus

发明人： Arno Deger ,  Roland Schenk ,  Gerhard Bienhaus

IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/563 ,  G01N33/58 ,  G01N33/78 ,  G01N33/536 ,  G01N33/532 ,  G01N33/542

CPC分类号： G01N33/58 ,  G01N33/53 ,  G01N33/54306 ,  G01N33/563 ,  G01N33/78

摘要： Method for the immunological determination of a ligand in which the ligand to be determined is reacted witha) at least 2 molecules of a specifically bindable substance P2 of which at least one of these molecules carries a label andb) a receptor R which consists of a binding partner P1 which is capable of monovalent binding to P2 and a binding site R.sub.1 for the ligand to be determined and then the label is determined,as well as a reagent for the immunological determination of a ligand which contains a specifically bindable substance P2 which is present at least partially in labelled form and a receptor R which consists of a binding partner P1 which is capable of monovalent binding to P2 and a binding site R.sub.1 for the ligand to be determined.

摘要翻译： 用于免疫测定配体的方法，其中待测定的配体与a）至少2分子的特异性可结合物质P2，其中至少一个这些分子带有标记，以及b）受体R，其由 能够与P2结合的结合配偶体P1和待测配体的结合位点R1，然后测定标记物，以及用于免疫测定含有特异性可结合物质P2的配体的试剂 至少部分以标记形式存在，受体R由能够与P2单价结合的结合配偶体P1和待测配位体的结合位点R1组成。

公开(公告)号：USD674504S1

公开(公告)日：2013-01-15

申请号：US29361079

申请日：2010-05-05

申请人： Roland Schenk

设计人： Roland Schenk

公开(公告)号：US20100028215A1

公开(公告)日：2010-02-04

申请号：US12312345

申请日：2007-11-14

申请人： Roland Schenk

发明人： Roland Schenk

IPC分类号： B01L3/00

CPC分类号： G01N35/1002 ,  B01L3/508 ,  B01L9/06

摘要： The present invention concerns a reagent container for liquid reagents comprising a substantially circular sector-shaped, trapezoidal or triangular container bottom and container top and container side walls which extend substantially vertically from the container bottom to the container top and which converge towards each other from a container rear wall to a container front side, wherein the container top has an opening. In order to be able to fit reagent containers of various sizes into a reagent carousel in exactly positioned and reliably fixed relationship it is proposed in accordance with the invention that provided at at least one container side wall of the reagent container is at least one positioning groove or positioning abutment surface which starts from the container bottom and extends vertically in the direction of the container top. In order to be able to use as many different reagent containers as possible for a given reagent carousel there is further proposed a reagent carousel having a substantially circular carousel base surface and compartments which are arranged radially thereon and which are defined by radially extending boundaries, for receiving reagent containers, wherein the boundaries defining a compartment have at least one projection facing towards the compartment interior for engagement with a positioning groove or a positioning abutment surface of a reagent container.

摘要翻译： 本发明涉及一种用于液体试剂的试剂容器，其包括基本上圆形的扇形，梯形或三角形容器底部以及容器顶部和容器侧壁，其从容器底部基本垂直延伸到容器顶部并且从一个 容器后壁到容器前侧，其中容器顶部具有开口。 为了能够将各种尺寸的试剂容器以精确定位和可靠的固定关系装配到试剂转盘中，根据本发明提出，在试剂容器的至少一个容器侧壁处设置有至少一个定位槽 或从容器底部开始并沿容器顶部的方向垂直延伸的定位邻接表面。 为了能够为给定的试剂转盘使用尽可能多的不同的试剂容器，还提出了一种试剂转盘，其具有基本圆形的转盘底座表面和隔室，其径向布置并且由径向延伸的边界限定，用于 接收试剂容器，其中限定隔室的边界具有至少一个朝向隔室内部的突出部，用于与试剂容器的定位槽或定位邻接表面接合。

公开(公告)号：US20130164857A1

公开(公告)日：2013-06-27

申请号：US13811959

申请日：2011-07-19

申请人： Roland Schenk

发明人： Roland Schenk

IPC分类号： B01L3/00

CPC分类号： B01L3/508 ,  B01F5/0647 ,  B01F13/0059 ,  B01F13/1016 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/027 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2300/0654 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2400/0487 ,  G01N21/05 ,  G01N21/648 ,  G01N2021/0346 ,  G01N2021/056

摘要： An interchangeable disposable measuring cassette for insertion into a measuring apparatus for detecting target molecules in a liquid sample by measuring fluorescence emission has a flow measurement cell in which an excitation radiation provided by the measuring apparatus produces an evanescent field in the liquid sample beyond a boundary layer for the liquid sample and the measurement cell. To be better able to ensure that no sample liquid can cross from the measurement cell into the measuring apparatus, the measuring cassette includes a body including an optically transparent material and a base in contact with the underside of the body. The measurement cell is formed by a cutout provided in the body, the base, or both. The areas on which the body and the base are on top of one another around this cutout are connected to one another directly and in fluid-tight fashion by laser welding.

公开(公告)号：US20120237397A1

公开(公告)日：2012-09-20

申请号：US13498929

申请日：2010-11-11

申请人： Roland Schenk

发明人： Roland Schenk

IPC分类号： G01N35/10

CPC分类号： G01N35/025 ,  G01N2035/0094 ,  G01N2035/0453

摘要： An automated analysis apparatus having sample, reagent and process rotors, at least three pipetting devices for transferring first and second reagents and a sample, a mixing device, a measuring device, and a flushing device. The analysis apparatus operates in successive operating cycles with the process rotor rotating about its axis of rotation. Simultaneously in each operating cycle in different process containers at different positions of the process rotor a sample is transferred into a process container, the first reagent is transferred into a process container, the second reagent is transferred into a process container, the process mixture is mixed in a process container, the measurement parameter of the process mixture in a process container is measured, and at least one process container is flushed. The rotation of the process rotor is stopped at most twice per operating cycle to transfer a sample and the first and second reagents into a process container.

摘要翻译： 具有样品，试剂和工艺转子的自动分析装置，用于转移第一和第二试剂的至少三个移液装置和样品，混合装置，测量装置和冲洗装置。 分析装置在连续的操作循环中操作，其中过程转子围绕其旋转轴线旋转。 在处理转子的不同位置的不同处理容器的每个操作循环中同时将样品转移到处理容器中，将第一试剂转移到处理容器中，将第二试剂转移到处理容器中，混合过程混合物 在处理容器中，测量处理容器中的处理混合物的测量参数，并且冲洗至少一个处理容器。 每个操作循环停止过程转子的旋转至多两次，以将样品和第一和第二试剂转移到处理容器中。