TOXIN ACTIVITY ASSAYS, DEVICES, METHODS AND SYSTEMS THEREFOR

    公开(公告)号:US20190195865A9

    公开(公告)日:2019-06-27

    申请号:US15056870

    申请日:2016-02-29

    IPC分类号: G01N33/53

    摘要: Embodiments of the present invention are directed toward devices, system and method for conducting toxin activity assay using sedimentation. The toxin activity assay may include generating complexes which bind to a plurality of beads in a fluid sample. The complexes may include a target toxin and a labeling agent, or may be generated due to presence of active target toxin and/or labeling agent designed to be incorporated into complexes responsive to the presence of target active toxin. The plurality of beads including the complexes may be transported through a density media, wherein the density media has a lower density than a density of the beads and higher than a density of the fluid sample, and wherein the transporting occurs, at least in part, by sedimentation. Signal may be detected from the labeling agents of the complexes.

    Scanning method for screening of electronic devices

    公开(公告)号:US10094874B1

    公开(公告)日:2018-10-09

    申请号:US15208931

    申请日:2016-07-13

    摘要: A visualization method for screening electronic devices is provided. In accordance with the disclosed method, a probe is applied to a grid of multiple points on the circuit, and an output produced by the circuit in response to the stimulus waveform is monitored for each of multiple grid points where the probe is applied. A power spectrum analysis (PSA) produces a power spectrum amplitude, in each of one or more frequency bins, on the monitored output for each of the multiple grid points. The PSA provides a respective pixel value for each of the multiple grid points. An image is displayed, in which image portions representing the multiple grid points are displayed with the respective pixel values.

    Method for enhancing hydrocarbon recovery from tight formations

    公开(公告)号:US10066471B2

    公开(公告)日:2018-09-04

    申请号:US14976102

    申请日:2014-12-23

    摘要: A method of increasing recovery of liquid hydrocarbons from subsurface reservoirs, and particularly from those located in tight formations, is disclosed. One aspect includes calculating the in situ fractured formation wettability from real-time measurement of flowback volume and composition. Another aspect includes determining the composition of the fracturing fluid, the overflush or both, that will achieve higher liquid hydrocarbon recovery by increasing the water wettability of rock surfaces within the reservoir. Monitoring of rock-surface wettability through flowback volume and composition profiles allows the above mentioned injectates to be adjusted in the field to achieve maximal recovery. Other methods, apparatuses, and systems are disclosed.

    SYSTEMS AND METHODS FOR LOCATING AND IMAGING PROPPANT IN AN INDUCED FRACTURE

    公开(公告)号:US20180210108A1

    公开(公告)日:2018-07-26

    申请号:US15935787

    申请日:2018-03-26

    摘要: Born Scattering Inversion (BSI) systems and methods are disclosed. A BSI system may be incorporated in a well system for accessing natural gas, oil and geothermal reserves in a geologic formation beneath the surface of the Earth. The BSI system may be used to generate a three-dimensional image of a proppant-filled hydraulically-induced fracture in the geologic formation. The BSI system may include computing equipment and sensors for measuring electromagnetic fields in the vicinity of the fracture before and after the fracture is generated, adjusting the parameters of a first Born approximation model of a scattered component of the surface electromagnetic fields using the measured electromagnetic fields, and generating the image of the proppant-filled fracture using the adjusted parameters.

    Detection of bioagents using a shear horizontal surface acoustic wave biosensor

    公开(公告)号:US10031135B2

    公开(公告)日:2018-07-24

    申请号:US14172429

    申请日:2014-02-04

    IPC分类号: G01N33/543 C12Q1/6825

    摘要: Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.