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    Detection of bioagents using a shear horizontal surface acoustic wave biosensor
    1.
    发明授权
    Detection of bioagents using a shear horizontal surface acoustic wave biosensor 有权

    公开(公告)号：US10031135B2

    公开(公告)日：2018-07-24

    申请号：US14172429

    申请日：2014-02-04

    申请人： STC.UNM ,  SANDIA CORPORATION

    发明人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC分类号： G01N33/543 ,  C12Q1/6825

    摘要： Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR
    2.
    发明申请
    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR 审中-公开

    公开(公告)号：US20190242888A1

    公开(公告)日：2019-08-08

    申请号：US16277618

    申请日：2019-02-15

    申请人： STC.UNM ,  SANDIA CORPORATION

    发明人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC分类号： G01N33/543 ,  G01N33/553 ,  C12Q1/6825 ,  G01N29/032

    CPC分类号： G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    摘要： Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR
    3.
    发明申请
    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR 审中-公开

    公开(公告)号：US20170184581A1

    公开(公告)日：2017-06-29

    申请号：US15411576

    申请日：2017-01-20

    申请人： STC.UNM ,  SANDIA CORPORATION

    发明人： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC分类号： G01N33/543 ,  G01N33/553 ,  G01N29/032 ,  C12Q1/68

    CPC分类号： G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    摘要： Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    High-frequency shear-horizontal surface acoustic wave sensor
    4.
    发明授权
    High-frequency shear-horizontal surface acoustic wave sensor 有权
    高频剪切水平声表面波传感器

    公开(公告)号：US08669688B1

    公开(公告)日：2014-03-11

    申请号：US13874692

    申请日：2013-05-01

    申请人： Sandia Corporation

    发明人： Darren W. Branch

    IPC分类号： H03H9/125

    CPC分类号： G01N29/022 ,  G01N29/036 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0423 ,  H03H9/02866 ,  H03H9/14505

    摘要： A Love wave sensor uses a single-phase unidirectional interdigital transducer (IDT) on a piezoelectric substrate for leaky surface acoustic wave generation. The IDT design minimizes propagation losses, bulk wave interferences, provides a highly linear phase response, and eliminates the need for impedance matching. As an example, a high frequency (˜300-400 MHz) surface acoustic wave (SAW) transducer enables efficient excitation of shear-horizontal waves on 36° Y-cut lithium tantalate (LTO) giving a highly linear phase response (2.8° P-P). The sensor has the ability to detect at the pg/mm2 level and can perform multi-analyte detection in real-time. The sensor can be used for rapid autonomous detection of pathogenic microorganisms and bioagents by field deployable platforms.

    摘要翻译： 爱波传感器在压电基板上使用单相单向叉指式传感器（IDT），以产生泄漏的表面声波。 IDT设计可最大限度地减少传播损耗，体波干扰，提供高度线性的相位响应，并消除对阻抗匹配的需要。 作为一个例子，高频（〜300-400 MHz）表面声波（SAW）传感器能够有效地激发36°Y切割的钽酸锂（LTO）上的剪切水平波，从而产生高度线性相位响应（2.8°PP ）。 该传感器能够以pg / mm2级别进行检测，并可实时执行多分析物检测。 该传感器可用于通过现场部署平台快速自主检测病原微生物和生物制剂。

    Miniature acoustic wave lysis system and uses thereof
    10.
    发明授权
    Miniature acoustic wave lysis system and uses thereof 有权
    微型声波裂解系统及其用途

    公开(公告)号：US09512421B1

    公开(公告)日：2016-12-06

    申请号：US14318364

    申请日：2014-06-27

    申请人： Sandia Corporation

    发明人： Darren W. Branch ,  Erika Cooley Vreeland ,  Gennifer Tanabe Smith

    IPC分类号： C12N13/00 ,  C12M1/33 ,  G01N29/036 ,  G01N29/02 ,  H03H9/145 ,  H03H9/02

    CPC分类号： C12N13/00 ,  C12N1/06 ,  G01N29/02 ,  G01N29/036 ,  G01N2291/0255 ,  G01N2291/0256 ,  G01N2291/0423 ,  H03H9/02866 ,  H03H9/14505

    摘要： The present invention relates to an acoustic lysis system including a disposable cartridge that can be reversibly coupled to a platform having a small, high-frequency piezoelectric transducer array. In particular, the system releases viable DNA, RNA, and proteins from human or bacterial cells, without chemicals or additional processing, to enable high-speed sample preparation for clinical point-of-care medical diagnostics and use with nano/microfluidic cartridges. Also described herein are methods of making and using the system of the invention.

    摘要翻译： 本发明涉及一种声学裂解系统，其包括可以可逆地耦合到具有小的高频压电换能器阵列的平台的一次性盒。 特别地，该系统从人或细菌细胞释放可行的DNA，RNA和蛋白质，无需化学品或额外处理，以实现临床点临床医疗诊断的高速样品制备和纳米/微流控盒的使用。 本文还描述了制造和使用本发明的系统的方法。