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    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR
    1.
    发明申请
    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR 审中-公开

    公开(公告)号：US20190242888A1

    公开(公告)日：2019-08-08

    申请号：US16277618

    申请日：2019-02-15

    Applicant: STC.UNM ,  SANDIA CORPORATION

    Inventor： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC: G01N33/543 ,  G01N33/553 ,  C12Q1/6825 ,  G01N29/032

    CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    Abstract: Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR
    2.
    发明申请
    DETECTION OF BIOAGENTS USING A SHEAR HORIZONTAL SURFACE ACOUSTIC WAVE BIOSENSOR 审中-公开

    公开(公告)号：US20170184581A1

    公开(公告)日：2017-06-29

    申请号：US15411576

    申请日：2017-01-20

    Applicant: STC.UNM ,  SANDIA CORPORATION

    Inventor： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC: G01N33/543 ,  G01N33/553 ,  G01N29/032 ,  C12Q1/68

    CPC classification number: G01N33/54373 ,  C12Q1/6825 ,  G01N29/032 ,  G01N33/553 ,  G01N2291/0422

    Abstract: Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    Detection of bioagents using a shear horizontal surface acoustic wave biosensor
    3.
    发明授权
    Detection of bioagents using a shear horizontal surface acoustic wave biosensor 有权

    公开(公告)号：US10031135B2

    公开(公告)日：2018-07-24

    申请号：US14172429

    申请日：2014-02-04

    Applicant: STC.UNM ,  SANDIA CORPORATION

    Inventor： Richard S. Larson ,  Brian Hjelle ,  Pam R. Hall ,  David C. Brown ,  Marco Bisoffi ,  Susan M. Brozik ,  Darren W. Branch ,  Thayne L. Edwards ,  David Wheeler

    IPC: G01N33/543 ,  C12Q1/6825

    Abstract: Viruses and other bioagents are of high medical and biodefense concern and their detection at concentrations well below the threshold necessary to cause health hazards continues to be a challenge with respect to sensitivity, specificity, and selectivity. Ideally, assays for accurate and real time detection of viral agents and other bioagents would not necessitate any pre-processing of the analyte, which would make them applicable for example to bodily fluids (blood, sputum) and man-made as well as naturally occurring bodies of water (pools, rivers). We describe herein a robust biosensor that combines the sensitivity of surface acoustic waves (SAW) generated at a frequency of 325 MHz with the specificity provided by antibodies and other ligands for the detection of viral agents. In preferred embodiments, a lithium tantalate based SAW transducer with silicon dioxide waveguide sensor platform featuring three test and one reference delay lines was used to adsorb antibodies directed against Coxsackie virus B4 or the negative-stranded category A bioagent Sin Nombre virus (SNV), a member of the genus Hantavirus, family Bunyaviridae, negative-stranded RNA viruses. Rapid detection (within seconds) of increasing concentrations of viral particles was linear over a range of order of magnitude for both viruses, although the sensor was approximately 50×104-fold more sensitive for the detection of SNV. For both pathogens, the sensor's selectivity for its target was not compromised by the presence of confounding Herpes Simplex virus type 1. The biosensor was able to detect SNV at doses lower than the load of virus typically found in a human patient suffering from hantavirus cardiopulmonary syndrome (HCPS). Further, in a proof-of-principle real world application, the SAW biosensor was capable of selectively detecting SNV agents in complex solutions, such as naturally occurring bodies of water (river, sewage effluent) without analyte pre-processing.

    APPARATUS COMPRISING MAGNETICALLY ACTUATED VALVES AND USES THEREOF
    6.
    发明申请
    APPARATUS COMPRISING MAGNETICALLY ACTUATED VALVES AND USES THEREOF 有权
    包括磁力驱动阀的装置及其用途

    公开(公告)号：US20150024377A1

    公开(公告)日：2015-01-22

    申请号：US14157335

    申请日：2014-01-16

    Applicant: Sandia Corporation

    Inventor： Thayne L. Edwards ,  Jason C. Harper

    IPC: F16K31/08 ,  C12Q1/04

    CPC classification number: G01N33/56911 ,  B01L3/502738 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0633 ,  F16K31/086 ,  F16K99/003 ,  F16K99/0046 ,  F16K2099/0084 ,  Y10T137/87917

    Abstract: The present invention, in part, relates to an apparatus having a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. In some examples, the valve is opened by bringing a magnet in proximity to the valve element to provide a magnetic force that delaminates the valve element from the adhesive coating. In particular, the apparatus can be useful for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings.

    Abstract translation: 本发明部分地涉及一种具有一次使用的常闭型流体阀的装置，其通过结合到粘合剂涂层的阀元件最初保持在关闭位置。 阀门使用磁力打开。 阀元件包括磁性材料或金属。 在一些示例中，通过使磁体靠近阀元件来打开阀，以提供使阀元件从粘合剂涂层分层的磁力。 特别地，该装置可用于片上放大和/或检测各种靶标，包括生物靶标和任何可扩增靶标。 这样的设备和方法对于现场或实时检测目标是有用的，特别是在有限的资源设置中。

    Apparatus comprising magnetically actuated valves and uses thereof
    9.
    发明授权
    Apparatus comprising magnetically actuated valves and uses thereof 有权
    装置包括磁力驱动阀及其用途

    公开(公告)号：US09389231B2

    公开(公告)日：2016-07-12

    申请号：US14157335

    申请日：2014-01-16

    Applicant: Sandia Corporation

    Inventor： Thayne L. Edwards ,  Jason C. Harper

    IPC: F16K31/06 ,  G01N33/569 ,  F16K31/08 ,  B01L3/00 ,  F16K99/00

    CPC classification number: G01N33/56911 ,  B01L3/502738 ,  B01L2300/0663 ,  B01L2300/0816 ,  B01L2300/0887 ,  B01L2400/0406 ,  B01L2400/0633 ,  F16K31/086 ,  F16K99/003 ,  F16K99/0046 ,  F16K2099/0084 ,  Y10T137/87917

    Abstract: The present invention, in part, relates to an apparatus having a single-use, normally-closed fluidic valve that is initially maintained in the closed position by a valve element bonded to an adhesive coating. The valve is opened using a magnetic force. The valve element includes a magnetic material or metal. In some examples, the valve is opened by bringing a magnet in proximity to the valve element to provide a magnetic force that delaminates the valve element from the adhesive coating. In particular, the apparatus can be useful for on-chip amplification and/or detection of various targets, including biological targets and any amplifiable targets. Such apparatuses and methods are useful for in-field or real-time detection of targets, especially in limited resource settings.

    Abstract translation: 本发明部分地涉及一种具有一次使用的常闭型流体阀的装置，其通过结合到粘合剂涂层的阀元件最初保持在关闭位置。 阀门使用磁力打开。 阀元件包括磁性材料或金属。 在一些示例中，通过使磁体靠近阀元件来打开阀，以提供使阀元件从粘合剂涂层分层的磁力。 特别地，该装置可用于片上放大和/或检测各种靶标，包括生物靶标和任何可扩增靶标。 这样的设备和方法对于现场或实时检测目标是有用的，特别是在有限的资源设置中。

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