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    Proteinaceous-substance-binding low-molecular-weight compound
    3.
    发明授权
    Proteinaceous-substance-binding low-molecular-weight compound 有权
    蛋白质 - 物质结合低分子量化合物

    公开(公告)号：US09273151B2

    公开(公告)日：2016-03-01

    申请号：US14007000

    申请日：2012-03-23

    申请人： Dai Murata ,  Shinichi Yoshida

    发明人： Dai Murata ,  Shinichi Yoshida

    IPC分类号： C07K1/14 ,  C07K17/00 ,  C07K1/22 ,  B01J20/289 ,  B01J20/32 ,  B01D15/38

    CPC分类号： C07K17/00 ,  B01D15/3809 ,  B01J20/289 ,  B01J20/3202 ,  B01J20/3219 ,  B01J20/3246 ,  C07K1/22

    摘要： The low-molecular-weight compound represented by the general formula (1): ArX-(Linker)-ArYHB (1), wherein ArX is a structure containing an optionally substituted aromatic six-membered ring, ArYHB is a structure containing an optionally substituted aromatic six-membered ring having a proton donor, the atom group “Linker” has not less than 4 and not more than 30 atoms and binds ArX with ArYHB, is used for binding a proteinaceous substance.

    摘要翻译： 由通式（1）表示的低分子量化合物：ArX-（连接子）-ArYHB（1），其中ArX是含有任意取代的芳香族六元环的结构，ArYHB是含有任意取代的 具有质子给体的芳族六元环，原子团“连接子”具有不少于4个且不多于30个的原子并与ArYHB结合ArX，用于结合蛋白质物质。

    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand
    5.
    发明授权
    Protein having affinity for immunoglobulin, and immunoglobulin-binding affinity ligand 有权
    对免疫球蛋白具有亲和力的蛋白质和免疫球蛋白结合亲和配体

    公开(公告)号：US09403883B2

    公开(公告)日：2016-08-02

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/31 ,  C07K14/47 ,  B01J20/286 ,  B01J20/32 ,  B01D15/38

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。

    PROTEIN FOR AFFINITY-SEPARATION MATRIX
    6.
    发明申请
    PROTEIN FOR AFFINITY-SEPARATION MATRIX 审中-公开
    蛋白质亲和分离矩阵

    公开(公告)号：US20140107315A1

    公开(公告)日：2014-04-17

    申请号：US14007225

    申请日：2012-03-26

    申请人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira

    发明人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira

    IPC分类号： C07K14/31 ,  C07K1/22

    CPC分类号： C07K14/31 ,  B01D15/3809 ,  B01J2220/4856 ,  C07K1/22 ,  C07K16/00 ,  C07K16/065 ,  C07K16/1271 ,  C07K17/00 ,  C07K17/12 ,  C07K2317/21 ,  C07K2317/52 ,  C07K2317/92

    摘要： An object of the present invention is to provide a technique to create novel engineered protein ligands that, when immobilized through a lysine residue (its side chain ε-amino group) which allows for efficient immobilization to a carrier, show the optimum binding capacity and binding efficiency to a target molecule. The present invention provides an engineered protein having a sequence obtained by replacing all the lysine residues in Protein A, which is the most typical protein ligand, with other amino acids, and adding lysine at a terminal; and an affinity separation matrix in which such an engineered protein is immobilized on a water-insoluble carrier by reductive amination or the like. This affinity separation matrix is characterized by its high binding capacity to a target molecule even when the immobilized amount of the ligand is small.

    摘要翻译： 本发明的一个目的是提供一种创建新型工程蛋白质配体的技术，当通过允许有效固定至载体的赖氨酸残基（其侧链和整合氨基）进行固定时，显示出最佳的结合能力， 目标分子的结合效率。 本发明提供了一种工程改造的蛋白质，其具有通过将最典型的蛋白质配体的蛋白A中的所有赖氨酸残基与其它氨基酸置换并在末端添加赖氨酸而获得的序列; 以及通过还原胺化等将这样的工程化蛋白质固定在水不溶性载体上的亲和分离基质。 该亲和分离基质的特征在于即使当配体的固定量小时，其对靶分子的结合能力也很高。

    PROTEIN CAPABLE OF BINDING SPECIFICALLY TO IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND
    7.
    发明申请
    PROTEIN CAPABLE OF BINDING SPECIFICALLY TO IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND 审中-公开
    蛋白质能够特异性结合免疫球蛋白和免疫调节蛋白结合亲和力配体

    公开(公告)号：US20130096276A1

    公开(公告)日：2013-04-18

    申请号：US13636584

    申请日：2011-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira ,  Masayuki Takano ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C07K14/195

    CPC分类号： C07K14/195 ,  C07K1/22 ,  C07K14/31 ,  C07K16/065 ,  C07K16/32 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/92

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the acidic condition compared with known engineered Protein A ligands. The present invention provides a protein having an affinity for an immunoglobulin, including an amino acid sequence obtained by introducing, into an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, at least one amino acid substitution at any one or more of amino acid residues corresponding to positions 31 to 37 of the A, B and C domains (positions 29 to 35 of the E domain, positions 34 to 40 of the D domain), which are conserved in all the domains, the protein having a lower affinity for an Fab region of an immunoglobulin than a protein having the amino acid sequence before introduction of the substitution.

    摘要翻译： 本发明的目的是与已知的工程改造的蛋白A配体相比，在酸性条件下产生具有更好抗体解离性能的新型工程蛋白A配体。 本发明提供对免疫球蛋白具有亲和性的蛋白质，包括通过将衍生自蛋白A​​的E，D，A，B和C结构域的任何氨基酸序列导入的氨基酸序列，至少一个氨基酸 在对应于A，B和C结构域（E结构域的29至35位，D结构域的34至40位）的31至37位的氨基酸残基的任何一个或多个取代，其在所有 结构域，对于免疫球蛋白Fab区域的亲和力低于在引入取代之前具有氨基酸序列的蛋白质的蛋白质。

    PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND
    8.
    发明申请
    PROTEIN HAVING AFFINITY FOR IMMUNOGLOBULIN, AND IMMUNOGLOBULIN-BINDING AFFINITY LIGAND 有权
    具有免疫球蛋白的免疫原性蛋白质和免疫球蛋白结合亲和力配体

    公开(公告)号：US20120208234A1

    公开(公告)日：2012-08-16

    申请号：US13258655

    申请日：2010-03-24

    申请人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    发明人： Shinichi Yoshida ,  Dai Murata ,  Masayuki Takano ,  Junya Akagi ,  Keita Iguchi ,  Yoshiyuki Nakano

    IPC分类号： C12P21/02 ,  C07K19/00 ,  C12N15/31 ,  C12N15/62 ,  C12N1/21 ,  C07K16/12 ,  C07K1/14 ,  C07K14/31 ,  C12N15/74

    CPC分类号： C07K14/47 ,  B01D15/3809 ,  B01J20/286 ,  B01J20/3244 ,  B01J20/3274 ,  C07K14/31

    摘要： An object of the present invention is to create a novel engineered Protein A ligand having better antibody dissociation properties in the presence of an acid than conventional engineered Protein A ligands and a further object of the present invention is to create a novel engineered Protein A ligand having higher alkali resistance. The present invention is to provide a protein having an affinity for an immunoglobulin, including an amino acid sequence derived from any of E, D, A, B and C domains of Protein A, wherein at least one Gly residue in the amino acid sequence is replaced with an amino acid other than Ala, and the protein has a lower affinity for an Fab region of an immunoglobulin than a protein including an amino acid sequence in which the Gly residue is replaced with Ala. Also, the present invention is to provide the protein having an affinity for an immunoglobulin, which has improved chemical stability in an alkaline condition compared to the corresponding domain.

    摘要翻译： 本发明的一个目的是在酸存在下产生具有比常规工程改造的蛋白A配体更好的抗体解离性能的新型工程改造的蛋白A配体，本发明的另一个目的是产生一种新的工程改造的蛋白A配体，其具有 耐碱性较高。 本发明提供一种对免疫球蛋白具有亲和性的蛋白质，其包括衍生自蛋白A​​的E，D，A，B和C结构域中的任一个的氨基酸序列，其中氨基酸序列中至少一个Gly残基为 被除Ala以外的氨基酸所取代，并且该蛋白质对包含氨基酸序列的蛋白质的免疫球蛋白Fab区域的亲和力低于其中Gly残基被Ala置换的蛋白质，本发明还提供 对于免疫球蛋白具有亲和力的蛋白质，与相应的结构域相比，其在碱性条件下具有改善的化学稳定性。

    Protein for affinity-separation matrix
    9.
    发明授权
    Protein for affinity-separation matrix 有权

    公开(公告)号：US10065995B2

    公开(公告)日：2018-09-04

    申请号：US14007225

    申请日：2012-03-26

    申请人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira

    发明人： Shinichi Yoshida ,  Dai Murata ,  Shunichi Taira

    IPC分类号： C07K14/31 ,  C07K1/22 ,  C07K16/00 ,  C07K16/12 ,  C07K17/12 ,  B01D15/38 ,  C07K16/06 ,  C07K17/00

    摘要： An object of the present invention is to provide a technique to create novel engineered protein ligands that, when immobilized through a lysine residue (its side chain ε-amino group) which allows for efficient immobilization to a carrier, show the optimum binding capacity and binding efficiency to a target molecule. The present invention provides an engineered protein having a sequence obtained by replacing all the lysine residues in Protein A, which is the most typical protein ligand, with other amino acids, and adding lysine at a terminal; and an affinity separation matrix in which such an engineered protein is immobilized on a water-insoluble carrier by reductive amination or the like. This affinity separation matrix is characterized by its high binding capacity to a target molecule even when the immobilized amount of the ligand is small.