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    Preparing method for (S)-3hydroxybutyric acid and (S)-3 hydroxybutyrate ester using recombinant microorganism
    3.
    发明授权
    Preparing method for (S)-3hydroxybutyric acid and (S)-3 hydroxybutyrate ester using recombinant microorganism 有权
    使用重组微生物制备（S）-3-羟基丁酸和（S）-3羟基丁酸酯的方法

    公开(公告)号：US08535918B2

    公开(公告)日：2013-09-17

    申请号：US12733026

    申请日：2008-06-10

    申请人： Si Jae Park ,  Sang Hyun Lee

    发明人： Si Jae Park ,  Sang Hyun Lee

    IPC分类号： C12P7/62

    CPC分类号： C12P7/62 ,  C12P7/42

    摘要： A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding β-ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding β-ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided.Accordingly, (S)-3-hydroxybutyric acid with high optical purity may be produced from acetyl CoA produced in glycolysis of a microorganism by a simple process involving the manipulation of a metabolic pathway by a recombinant gene introduced into the microorganism without using a high-cost metal catalyst or a substrate. Further, (S)-3-hydroxybutyrate ester and lactone of (S)-3-hydroxybutyrate ester may be simply produced from (S)-3-hydroxybutyric acid produced by the above method using lipase.

    摘要翻译： 提供了使用突变的微生物合成光学活性（S）-3-羟基丁酸和（S）-3-羟基丁酸酯的方法。 更具体地，涉及制备用编码β-酮硫解酶的基因转化的（S）-3-羟基丁酸的突变微生物，编码（S）-3-羟基丁酰辅酶A脱氢酶的基因和编码酰基辅酶A水解酶的基因; 使用突变的微生物制备（S）-3-羟基丁酸的方法; 用于制备用编码β-酮硫解酶的基因转化的（S）-3-羟基丁酸酯的突变微生物，编码（S）-3-羟基丁酰辅酶A脱氢酶的基因，编码酰基辅酶A水解酶的基因和编码脂肪酶的基因; 并提供使用突变微生物制备（S）-3-羟基丁酸酯的方法。 因此，具有高光学纯度的（S）-3-羟基丁酸可以通过涉及通过引入微生物的重组基因操纵代谢途径的简单方法由乙醇辅酶A生产的乙酰辅酶A产生， 成本金属催化剂或基材。 此外，（S）-3-羟基丁酸酯的（S）-3-羟基丁酸酯和内酯可以简单地由使用脂肪酶的上述方法制备的（S）-3-羟基丁酸生成。

    Cells or plants producing polylactate or its copolymers and uses thereof
    8.
    发明申请
    Cells or plants producing polylactate or its copolymers and uses thereof 有权
    产生聚乳酸或其共聚物的细胞或植物及其用途

    公开(公告)号：US20070277268A1

    公开(公告)日：2007-11-29

    申请号：US11439517

    申请日：2006-05-24

    申请人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

    发明人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

    IPC分类号： A01H5/00 ,  C12P7/62 ,  C12N1/21 ,  C08L77/06

    CPC分类号： C12P7/625 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/93 ,  C12N15/70 ,  C12N15/74 ,  C12N15/8242 ,  C12N15/8243 ,  C12N2500/35 ,  Y02P20/52

    摘要： The present invention relates to cells or plants that can produce polylactate or its copolymers and to a method for preparing polylactate or its copolymers using the same. More specifically, cells or plants with the ability to produce polylactate or hydroxyalkanoate-lactate copolymers comprise both a gene encoding an enzyme that converts lactate into lactyl-CoA and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses lactyl-CoA as a substrate. Also described is a method for preparing polylactate or hydroxyalkanoate-lactate copolymers which comprises culturing the cells in a medium containing lactate or lactate and various hydroxyalkanoates or culturing the plants. Effective preparation of hydroxyalkanoate-lactate copolymer which comprises various hydroxyalkanoates as well as polylactate, using the cells or the plants, is disclosed.

    摘要翻译： 本发明涉及可产生聚乳酸或其共聚物的细胞或植物，以及使用其制备聚乳酸或其共聚物的方法。 更具体地说，具有产生聚乳酸或羟基链烷酸酯 - 乳酸酯共聚物能力的细胞或植物包括编码将乳酸转化为乳酰基-CoA的酶的基因和使用乳酰CoA作为底物的编码聚羟基链烷酸酯（PHA）合酶的基因。 还描述了制备聚乳酸或羟基链烷酸酯 - 乳酸共聚物的方法，其包括在含有乳酸盐或乳酸盐和各种羟基链烷酸酯的培养基中培养细胞或培养植物。 公开了使用细胞或植物有效制备包含各种羟基链烷酸酯以及聚乳酸的羟基链烷酸酯 - 乳酸共聚物。

    Rhodococcus - E. coli shuttle vector
    9.
    发明申请
    Rhodococcus - E. coli shuttle vector 审中-公开
    红球菌 - 大肠杆菌穿梭载体

    公开(公告)号：US20060263868A1

    公开(公告)日：2006-11-23

    申请号：US11355198

    申请日：2006-02-16

    申请人： Sanghyun Lee ,  Junhyeong Cho ,  Ohjin Park ,  Joowon Rhee ,  Si Jae Park

    发明人： Sanghyun Lee ,  Junhyeong Cho ,  Ohjin Park ,  Joowon Rhee ,  Si Jae Park

    IPC分类号： C12N1/21 ,  C12N15/74

    CPC分类号： C12N15/70 ,  C12N15/74

    摘要： A novel Rhodococcus-E. coli shuttle vector comprising an origin of replication of a novel plasmid derived from Rhodococcus erythropolis IAM1484 and an origin of replication derived from E. coli, is disclosed. This shuttle vector replicates bidirectionally in E. coli and Rhodococcus. Also disclosed is a recombinant vector in which a target gene is operably linked to the shuttle vector, and microorganisms transformed with the recombinant vector. The shuttle vector is relatively small in size, can replicate in a variety of species of the genus Rhodococcus and has high replication stability in a host cell. Therefore, this shuttle vector is useful for cloning a variety of genes.

    摘要翻译： 一种新型红球菌 公开了包含衍生自红球菌红血球IAM1484的新质粒的复制起点和源自大肠杆菌的复制起点的大肠杆菌穿梭载体。 这种穿梭载体在大肠杆菌和红球菌中双向复制。 还公开了重组载体，其中靶基因与穿梭载体可操作地连接，并用重组载体转化微生物。 穿梭载体的尺寸相对较小，可以在红球菌属的各种物种中复制，并且在宿主细胞中具有高的复制稳定性。 因此，该穿梭载体可用于克隆各种基因。

    Cells or plants producing polylactate or its copolymers and uses thereof
    10.
    发明授权
    Cells or plants producing polylactate or its copolymers and uses thereof 有权
    产生聚乳酸或其共聚物的细胞或植物及其用途

    公开(公告)号：US09410167B2

    公开(公告)日：2016-08-09

    申请号：US11439517

    申请日：2006-05-24

    申请人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

    发明人： Jun-Hyeong Cho ,  Si-Jae Park ,  Sang-Yup Lee ,  Yu-Kyung Jung

    IPC分类号： C12N15/70 ,  C12N15/74 ,  C12P7/62 ,  C12N9/10 ,  C12N9/00 ,  C12N15/82

    CPC分类号： C12P7/625 ,  C12N9/10 ,  C12N9/1025 ,  C12N9/93 ,  C12N15/70 ,  C12N15/74 ,  C12N15/8242 ,  C12N15/8243 ,  C12N2500/35 ,  Y02P20/52

    摘要： The present invention relates to cells or plants that can produce polylactate or its copolymers and to a method for preparing polylactate or its copolymers using the same. More specifically, cells or plants with the ability to produce polylactate or hydroxyalkanoate-lactate copolymers comprise both a gene encoding an enzyme that converts lactate into lactyl-CoA and a gene encoding polyhydroxyalkanoate (PHA) synthase which uses lactyl-CoA as a substrate. Also described is a method for preparing polylactate or hydroxyalkanoate-lactate copolymers which comprises culturing the cells in a medium containing lactate or lactate and various hydroxyalkanoates or culturing the plants. Effective preparation of hydroxyalkanoate-lactate copolymer which comprises various hydroxyalkanoates as well as polylactate, using the cells or the plants, is disclosed.

    摘要翻译： 本发明涉及可产生聚乳酸或其共聚物的细胞或植物，以及使用其制备聚乳酸或其共聚物的方法。 更具体地说，具有产生聚乳酸或羟基链烷酸酯 - 乳酸酯共聚物能力的细胞或植物包括编码将乳酸转化为乳酰基-CoA的酶的基因和使用乳酰CoA作为底物的编码聚羟基链烷酸酯（PHA）合酶的基因。 还描述了制备聚乳酸或羟基链烷酸酯 - 乳酸共聚物的方法，其包括在含有乳酸盐或乳酸盐和各种羟基链烷酸酯的培养基中培养细胞或培养植物。 公开了使用细胞或植物有效制备包含各种羟基链烷酸酯以及聚乳酸的羟基链烷酸酯 - 乳酸共聚物。