公开(公告)号：US08685701B2

公开(公告)日：2014-04-01

申请号：US13147572

申请日：2010-02-03

申请人： Taek Ho Yang ,  Si Jae Park ,  Eun Jeong Lee ,  Hye Ok Kang ,  Tae Wan Kim ,  Sang Hyun Lee

发明人： Taek Ho Yang ,  Si Jae Park ,  Eun Jeong Lee ,  Hye Ok Kang ,  Tae Wan Kim ,  Sang Hyun Lee

IPC分类号： C12N1/20 ,  C12N9/10 ,  C12N15/00 ,  C12P7/62 ,  C07H21/04

CPC分类号： C12P7/56 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N15/74 ,  C12P7/62 ,  C12P7/625 ,  C12Y101/01036 ,  C12Y203/01 ,  C12Y208/03001

摘要： Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.

摘要翻译： 本发明提供能够生产聚乳酸或羟基链烷酸酯 - 乳酸共聚物的重组Ralstonia eutropha，以及使用其制备聚乳酸酯或羟基链烷酸酯 - 乳酸共聚物的方法。 可以培养通过将乳酸酯转化为乳酰基-CoA的酶的基因和使用乳酰基辅酶A作为底物的聚羟基链烷酸酯（PHA）合酶的基因引入的重组Ralstonia eutropha，从而有效地制备乳酸聚合物 和乳酸共聚物。

公开(公告)号：US20120045801A1

公开(公告)日：2012-02-23

申请号：US13147572

申请日：2010-02-03

申请人： Taek Ho Yang ,  Si Jae Park ,  Eun Jeong Lee ,  Hye Ok Kang ,  Tae Wan Kim ,  Sang Hyun Lee

发明人： Taek Ho Yang ,  Si Jae Park ,  Eun Jeong Lee ,  Hye Ok Kang ,  Tae Wan Kim ,  Sang Hyun Lee

IPC分类号： C12P7/62 ,  C12N1/21

CPC分类号： C12P7/56 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/13 ,  C12N15/74 ,  C12P7/62 ,  C12P7/625 ,  C12Y101/01036 ,  C12Y203/01 ,  C12Y208/03001

摘要： Provided are a recombinant Ralstonia eutropha capable of producing polylactate or a hydroxyalkanoate-lactate copolymer, and a method of preparing polylactate or a hydroxyalkanoate-lactate copolymer using the same. The recombinant Ralstonia eutropha, which is prepared by introducing a gene of an enzyme converting lactate into lactyl-CoA and a gene of a polyhydroxyalkanoate (PHA) synthase using lactyl-CoA as a substrate thereto, may be cultured, thereby efficiently preparing a lactate polymer and a lactate copolymer.

摘要翻译： 本发明提供能够生产聚乳酸或羟基链烷酸酯 - 乳酸共聚物的重组Ralstonia eutropha，以及使用其制备聚乳酸酯或羟基链烷酸酯 - 乳酸共聚物的方法。 可以培养通过将乳酸酯转化为乳酰基-CoA的酶的基因和使用乳酰基辅酶A作为底物的聚羟基链烷酸酯（PHA）合酶的基因引入的重组Ralstonia eutropha，从而有效地制备乳酸聚合物 和乳酸共聚物。

公开(公告)号：US08524478B2

公开(公告)日：2013-09-03

申请号：US12673389

申请日：2008-07-25

申请人： Si Jae Park ,  Taek Ho Yang ,  Hye Ok Kang ,  Sang Hyun Lee ,  Eun Jung Lee ,  Tae Wan Kim

发明人： Si Jae Park ,  Taek Ho Yang ,  Hye Ok Kang ,  Sang Hyun Lee ,  Eun Jung Lee ,  Tae Wan Kim

IPC分类号： C12N1/10 ,  C12P7/62 ,  C07H21/04

CPC分类号： C12P7/625 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/13 ,  C12Y203/01 ,  C12Y208/03001 ,  Y02P20/52

摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.

摘要翻译： 本发明提供一种丙酸酰辅酶A转移酶突变体，其可从丙酸梭菌中在使用微生物制备聚乳酸（PLA）或PLA共聚物的方法中以高效率将乳酸转化为乳酰辅酶A。 不同于在大肠杆菌中弱表达的常规丙酰基-CoA转移酶，当将来自丙酸梭菌的丙酰辅酶A转移酶的突变体引入重组大肠杆菌中时，可以非常顺利地提供乳酰辅酶A，从而能够高效制备聚乳酸 （PLA）和PLA共聚物。

公开(公告)号：US08883476B2

公开(公告)日：2014-11-11

申请号：US13952355

申请日：2013-07-26

申请人： Si Jae Park ,  Taek Ho Yang ,  Hye Ok Kang ,  Sang Hyun Lee ,  Eun Jung Lee ,  Tae Wan Kim

发明人： Si Jae Park ,  Taek Ho Yang ,  Hye Ok Kang ,  Sang Hyun Lee ,  Eun Jung Lee ,  Tae Wan Kim

IPC分类号： C12N1/10 ,  C12P7/62 ,  C07H21/04 ,  C12N9/10

CPC分类号： C12P7/625 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/13 ,  C12Y203/01 ,  C12Y208/03001 ,  Y02P20/52

摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.

摘要翻译： 本发明提供一种丙酸酰辅酶A转移酶突变体，其可从丙酸梭菌中在使用微生物制备聚乳酸（PLA）或PLA共聚物的方法中以高效率将乳酸转化为乳酰辅酶A。 不同于在大肠杆菌中弱表达的常规丙酰基-CoA转移酶，当将来自丙酸梭菌的丙酰辅酶A转移酶的突变体引入重组大肠杆菌中时，可以非常顺利地提供乳酰辅酶A，从而能够高效制备聚乳酸 （PLA）和PLA共聚物。

公开(公告)号：US20110177569A1

公开(公告)日：2011-07-21

申请号：US12673389

申请日：2008-07-25

申请人： Si Jae Park ,  Taek Ho Yang ,  Hye Ok Kang ,  Sang Hyun Lee ,  Eun Jung Lee ,  Tae Wan Kim

发明人： Si Jae Park ,  Taek Ho Yang ,  Hye Ok Kang ,  Sang Hyun Lee ,  Eun Jung Lee ,  Tae Wan Kim

IPC分类号： C12P7/62 ,  C12N9/10 ,  C12N15/54 ,  C12N15/82 ,  A01H5/00

CPC分类号： C12P7/625 ,  C12N9/1029 ,  C12N9/1048 ,  C12N9/13 ,  C12Y203/01 ,  C12Y208/03001 ,  Y02P20/52

摘要： Provided is a mutant of propionyl-CoA transferase from Clostridium propionicum that can convert lactate into lactyl-CoA with high efficiency in a method of preparing a polylactate (PLA) or PLA copolymer using microorganisms. Unlike conventional propionyl-CoA transferase which is weakly expressed in E. coli, when a mutant of propiony-CoA transferase from Clostridium propionicum is introduced into recombinant E. coli, lactyl-CoA can be supplied very smoothly, thereby enabling highly efficient preparation of polylactate (PLA) and PLA copolymer.

摘要翻译： 本发明提供一种丙酸酰辅酶A转移酶突变体，其可从丙酸梭菌中在使用微生物制备聚乳酸（PLA）或PLA共聚物的方法中以高效率将乳酸转化为乳酰辅酶A。 不同于在大肠杆菌中弱表达的常规丙酰基-CoA转移酶，当将来自丙酸梭菌的丙酰辅酶A转移酶的突变体引入重组大肠杆菌中时，可以非常顺利地提供乳酰辅酶A，从而能够高效制备聚乳酸 （PLA）和PLA共聚物。

公开(公告)号：US20110201067A1

公开(公告)日：2011-08-18

申请号：US13001001

申请日：2009-06-24

申请人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Si Jae Park ,  Tae Wan Kim

发明人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Si Jae Park ,  Tae Wan Kim

IPC分类号： C12P7/62

CPC分类号： C08G63/06 ,  C12P7/625 ,  C12P21/02

摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

摘要翻译： 提供了使用突变微生物制备聚乳酸（PLA）或其共聚物的方法，其中遗传操作参与辅酶A（CoA）供体和乳酸生产途径的基因以提高CoA供体和乳酸的生产率 。 CoA供体和乳酸盐的量在微生物代谢途径中同时增加，以有效生物合成PLA和具有高含量乳酸盐的羟基链烷酸酯 - 乳酸共聚物，其在工业上是有用的。

公开(公告)号：US09120891B2

公开(公告)日：2015-09-01

申请号：US13001001

申请日：2009-06-24

申请人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Si Jae Park ,  Tae Wan Kim

发明人： Sang Yup Lee ,  Yu Kyung Jung ,  Taek Ho Yang ,  Si Jae Park ,  Tae Wan Kim

IPC分类号： C12P7/18 ,  C12P7/42 ,  C12N1/20 ,  C12N15/74 ,  C07H21/04 ,  C08G63/06 ,  C12P7/62 ,  C12P21/02

CPC分类号： C08G63/06 ,  C12P7/625 ,  C12P21/02

摘要： Provided is a method of preparing polylactate (PLA) or a copolymer thereof using a mutant microorganism in which a gene participating in a coenzyme A (CoA) donor- and lactate-producing pathway is genetically manipulated to increase the productivity of a CoA donor and lactate. Amounts of the CoA donor and the lactate are simultaneously increased in a microbial metabolic pathway to enable effective biosynthesis of PLA and a hydroxyalkanoate-lactate copolymer having a high content of lactate, which is industrially useful.

摘要翻译： 提供了使用突变微生物制备聚乳酸（PLA）或其共聚物的方法，其中遗传操作参与辅酶A（CoA）供体和乳酸生产途径的基因以提高CoA供体和乳酸的生产率 。 CoA供体和乳酸盐的量在微生物代谢途径中同时增加，以有效生物合成PLA和具有高含量乳酸盐的羟基链烷酸酯 - 乳酸共聚物，其在工业上是有用的。

公开(公告)号：US20100330634A1

公开(公告)日：2010-12-30

申请号：US12676840

申请日：2008-08-13

申请人： Si Jae Park ,  Sang Hyun Lee ,  Sang Yup Lee ,  Eun Jeong Lee

发明人： Si Jae Park ,  Sang Hyun Lee ,  Sang Yup Lee ,  Eun Jeong Lee

IPC分类号： C12P7/18 ,  C12N1/19 ,  C12N1/15 ,  C12N1/21 ,  C07H21/04 ,  C12N15/63

CPC分类号： C12N15/77 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/001 ,  C12N9/13 ,  C12P7/18 ,  C12Y101/01061 ,  C12Y102/01024 ,  C12Y103/99002

摘要： A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process.

摘要翻译： 提供能够生产1,4-丁二醇的突变体和使用其制备1,4-丁二醇的方法。 通过在能够产生琥珀酸的微生物中引入和扩增编码将琥珀酸酯转化成4-羟基丁酸酯和4-羟基丁酸酯的基因转化成1,4-丁二醇来制备突变体微生物。 该方法包括在含有碳水化合物的培养基中培养该突变体并从培养物中获得1,4-丁二醇。 因此，在化学工业中必不可少的1,4-丁二醇可以在生物过程中制备。

公开(公告)号：US08535918B2

公开(公告)日：2013-09-17

申请号：US12733026

申请日：2008-06-10

申请人： Si Jae Park ,  Sang Hyun Lee

发明人： Si Jae Park ,  Sang Hyun Lee

IPC分类号： C12P7/62

CPC分类号： C12P7/62 ,  C12P7/42

摘要： A method of synthesizing optically-active (S)-3-hydroxybutyric acid and (S)-3-hydroxybutyrate ester using a mutated microorganism is provided. More particularly, a mutated microorganism for preparing (S)-3-hydroxybutyric acid transformed with a gene encoding β-ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase and a gene encoding acyl CoA hydrolase; a method of preparing (S)-3-hydroxybutyric acid using the mutated microorganism; a mutated microorganism for preparing (S)-3-hydroxybutyrate ester transformed with a gene encoding β-ketothiolase, a gene encoding (S)-3-hydroxybutyryl CoA dehydrogenase, a gene encoding acyl CoA hydrolase and a gene encoding lipase; and a method of preparing (S)-3-hydroxybutyrate ester using the mutated microorganism are provided.Accordingly, (S)-3-hydroxybutyric acid with high optical purity may be produced from acetyl CoA produced in glycolysis of a microorganism by a simple process involving the manipulation of a metabolic pathway by a recombinant gene introduced into the microorganism without using a high-cost metal catalyst or a substrate. Further, (S)-3-hydroxybutyrate ester and lactone of (S)-3-hydroxybutyrate ester may be simply produced from (S)-3-hydroxybutyric acid produced by the above method using lipase.

摘要翻译： 提供了使用突变的微生物合成光学活性（S）-3-羟基丁酸和（S）-3-羟基丁酸酯的方法。 更具体地，涉及制备用编码β-酮硫解酶的基因转化的（S）-3-羟基丁酸的突变微生物，编码（S）-3-羟基丁酰辅酶A脱氢酶的基因和编码酰基辅酶A水解酶的基因; 使用突变的微生物制备（S）-3-羟基丁酸的方法; 用于制备用编码β-酮硫解酶的基因转化的（S）-3-羟基丁酸酯的突变微生物，编码（S）-3-羟基丁酰辅酶A脱氢酶的基因，编码酰基辅酶A水解酶的基因和编码脂肪酶的基因; 并提供使用突变微生物制备（S）-3-羟基丁酸酯的方法。 因此，具有高光学纯度的（S）-3-羟基丁酸可以通过涉及通过引入微生物的重组基因操纵代谢途径的简单方法由乙醇辅酶A生产的乙酰辅酶A产生， 成本金属催化剂或基材。 此外，（S）-3-羟基丁酸酯的（S）-3-羟基丁酸酯和内酯可以简单地由使用脂肪酶的上述方法制备的（S）-3-羟基丁酸生成。

公开(公告)号：US07183087B2

公开(公告)日：2007-02-27

申请号：US10662358

申请日：2003-09-16

申请人： Sang Yup Lee ,  Si Jae Park

发明人： Sang Yup Lee ,  Si Jae Park

IPC分类号： C12P7/62 ,  C12N1/20 ,  C12N9/88 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12N9/93 ,  C08G63/06 ,  C12N9/88 ,  C12P7/625 ,  C12Y402/01017

摘要： The present invention relates to a method for producing middle-chain-length polyhydroxyalkanoate (MCL-PHA) using a maoC gene. The producing method of MCL-PHA according to the present invention comprises the steps of transforming a microorganism with the maoC gene to give a transformant, the microorganism being deleted of a fadB gene and containing a PHA synthase gene; culturing the transformant in medium containing a C6-10 carbon source; and obtaining PHA consisting of monomers with 6–10 carbon atoms. When the maoC gene whose function has not yet been established is used according to the present invention, high quality PHA with a higher number of carbon atoms than the prior PHA can be produced at a higher efficiency.

摘要翻译： 本发明涉及使用maoC基因生产中链长度聚羟基链烷酸酯（MCL-PHA）的方法。 根据本发明的MCL-PHA的制备方法包括以下步骤：用maoC基因转化微生物以产生转化体，所述微生物缺失fadB基因并含有PHA合酶基因; 在含有C 6-10碳源的培养基中培养转化体; 并获得由具有6-10个碳原子的单体组成的PHA。 当根据本发明使用尚未建立功能的maoC基因时，可以以更高的效率生产具有比先前的PHA高的碳原子数的高质量PHA。