Lithographic method for attaching biological cells to a solid substrate using a small molecule linker
    1.
    发明申请
    Lithographic method for attaching biological cells to a solid substrate using a small molecule linker 审中-公开
    使用小分子接头将生物细胞附着到固体底物的平版印刷方法

    公开(公告)号:US20050136538A1

    公开(公告)日:2005-06-23

    申请号:US10739493

    申请日:2003-12-17

    IPC分类号: C12N5/00 C12N5/02

    摘要: One embodiment of the invention describes a novel method for providing a substrate for selective cell patterning, wherein the method comprises contacting an epoxide coated substrate surface with a bi-functional molecule having an epoxide end group and reacting these end groups with the substrate surface through a photochemically induced acid coupling reaction. The bi-functional molecule is applied in solution to the substrate surface as a photo-sensitive coating. A photomask stencil is used to deposit electromagnetic radiation into the coating in predetermined locations to form a desired pattern of the coating. The patterned substrate is provided by washing coating from the substrate leaving the bi-functional molecule attached to the substrate in those areas exposed to the radiation providing thereby cell adhesive moieties in controlled locations on the substrate.

    摘要翻译: 本发明的一个实施方案描述了一种用于提供用于选择性细胞图案化的底物的新方法,其中所述方法包括使环氧化物涂覆的基底表面与具有环氧端基的双功能分子接触,并使这些端基与基底表面反应,通过 光化学诱导酸偶联反应。 将双功能分子作为光敏涂层施加在基材表面的溶液中。 光掩模模板用于将电磁辐射沉积到预定位置的涂层中以形成涂层的期望图案。 通过从衬底洗涤涂层而提供图案化衬底,留下暴露于辐射的那些区域中附着到衬底上的双功能分子,从而提供衬底上受控位置中的细胞粘合剂部分。