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公开(公告)号:US08609348B2
公开(公告)日:2013-12-17
申请号:US13002465
申请日:2009-07-03
申请人: Manfred Biebl , Renate Grassl , Monika Walter , Stefan Miller
发明人: Manfred Biebl , Renate Grassl , Monika Walter , Stefan Miller
CPC分类号: C07K14/005 , C07K2319/21 , C07K2319/22 , C07K2319/40 , C12N2700/00 , C12N2795/10322 , G01N33/56911 , G01N2333/005
摘要: Bacteriophage adhesion proteins binding to the O-antigen of gram negative bacteria, but lacking the ability of binding to a bacteriophage and of hydrolysing lipopolysaccharides, are described, as well as nucleic acid molecules encoding the proteins. In addition, methods for generating the bacteriophage adhesion proteins and their use in detection, purification and enrichment of bacteria are described.
摘要翻译: 描述了与革兰氏阴性细菌的O-抗原结合但是缺乏结合噬菌体和水解脂多糖的能力的噬菌体粘附蛋白以及编码蛋白质的核酸分子。 此外,描述了用于产生噬菌体粘附蛋白的方法及其在细菌的检测,纯化和富集中的用途。
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公开(公告)号:US20100028857A1
公开(公告)日:2010-02-04
申请号:US12510540
申请日:2009-07-28
申请人: Michael Schütz , Roman Meyer , Holger Grallert , Stefan Miller
发明人: Michael Schütz , Roman Meyer , Holger Grallert , Stefan Miller
CPC分类号: G01N33/569 , G01N33/566
摘要: The invention relates to a method for identifying endotoxins for eliminating said endotoxins from a sample, with the aid of bacteriophage tail proteins.
摘要翻译: 本发明涉及用噬菌体尾蛋白鉴定内毒素从样品中去除所述内毒素的方法。
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公开(公告)号:US20070233728A1
公开(公告)日:2007-10-04
申请号:US11396252
申请日:2006-03-30
申请人: Joachim Puteick , Martin Rogge , Hendrik Geipel , Andrea Pluemper , Andreas Huppert , Kai-Uwe Pielka , Ashwin Yeddula , Matthias Schmitt , Toralf Grossmann , Andreas Neumann , Bare Said , Stefan Miller , Michael Neumann , Florian Rehfeld , Ingo Bruss , Horst Schaude , Michael Bauer , Andreas Morsch , Silke Storch , Klaus Bahr , Bernd Schmitt , Michael Meyringer , Dirk Henrich
发明人: Joachim Puteick , Martin Rogge , Hendrik Geipel , Andrea Pluemper , Andreas Huppert , Kai-Uwe Pielka , Ashwin Yeddula , Matthias Schmitt , Toralf Grossmann , Andreas Neumann , Bare Said , Stefan Miller , Michael Neumann , Florian Rehfeld , Ingo Bruss , Horst Schaude , Michael Bauer , Andreas Morsch , Silke Storch , Klaus Bahr , Bernd Schmitt , Michael Meyringer , Dirk Henrich
IPC分类号: G06F7/00
CPC分类号: G06Q10/06
摘要: Methods and apparatus, including systems and computer program products, for a services architecture design that provides enterprise services having foundation layer functionality at the level of an enterprise application. The design includes a set of service operations, process components, and optionally deployment units. Suitable business objects are also described.
摘要翻译: 用于服务架构设计的方法和装置,包括系统和计算机程序产品,其提供在企业应用级别具有基础层功能的企业服务。 该设计包括一组服务操作,过程组件和可选的部署单元。 还描述了合适的业务对象。
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公开(公告)号:US08906365B2
公开(公告)日:2014-12-09
申请号:US13380312
申请日:2010-06-28
申请人: Rob Lavigne , Stefan Miller , Yves Briers , Guido Volckaert , Maarten Walmagh
发明人: Rob Lavigne , Stefan Miller , Yves Briers , Guido Volckaert , Maarten Walmagh
IPC分类号: C12N9/24 , C07K14/005 , C07K14/47 , C07K19/00 , A61K38/47 , A61L2/00 , C12N15/56 , C12N15/62 , C12N9/50 , C12N15/52 , C12N9/36 , A61K38/00
CPC分类号: A61K38/47 , A61K38/00 , A61L2/00 , C07K14/005 , C07K14/4723 , C07K2319/00 , C07K2319/20 , C12N9/2462 , C12N9/503 , C12N15/52 , C12N15/62 , C12Y302/01017 , Y02A50/475 , Y02A50/478 , Y02A50/479
摘要: The application relates to antimicrobial agents against Gram-negative bacteria, in particular to fusion proteins composed of an enzyme having the activity of degrading the cell wall of Gram-negative bacteria and a peptide stretch fused to the enzyme at the N- or C-terminus, as well as pharmaceutical compositions thereof. Moreover, it relates to nucleic acid molecules encoding such a fusion protein, vectors carrying the nucleic acid molecules and host cells transformed with either the nucleic acid molecules or the vectors. In addition, it relates to such a fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means or as cosmetic substance. The application also relates to the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries.
摘要翻译: 本申请涉及抗革兰氏阴性细菌的抗微生物剂,特别是由具有降解革兰氏阴性细菌细胞壁的活性的酶和在N-或C-末端与酶融合的肽拉伸组成的融合蛋白 ,以及其药物组合物。 此外,它涉及编码这种融合蛋白的核酸分子,携带核酸分子的载体和用核酸分子或载体转化的宿主细胞。 此外,它涉及用作药物,特别是用于治疗或预防革兰氏阴性细菌感染的这种融合蛋白,作为诊断手段或化妆品。 该应用还涉及食品加工设备,食品加工厂,与食品接触的表面,医疗器械,医院和手术中的表面的革兰氏阴性细菌污染的治疗或预防。
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公开(公告)号:US08394597B2
公开(公告)日:2013-03-12
申请号:US10583415
申请日:2004-12-20
申请人: Roman Meyer , Michael Schütz , Holger Grallert , Renate Grassl , Stefan Miller
发明人: Roman Meyer , Michael Schütz , Holger Grallert , Renate Grassl , Stefan Miller
CPC分类号: G01N33/92 , G01N33/56911 , G01N2400/50 , Y10T436/10
摘要: The invention relates to a method for detecting endotoxins in a sample.
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公开(公告)号:US20120189608A1
公开(公告)日:2012-07-26
申请号:US13390033
申请日:2010-08-24
申请人: Yves Briers , Rob Lavigne , Maarten Walmagh , Stefan Miller
发明人: Yves Briers , Rob Lavigne , Maarten Walmagh , Stefan Miller
IPC分类号: A61K38/47 , C07K14/21 , C12N1/21 , C12N1/19 , C12N5/10 , A01P1/00 , C12N15/63 , C12N15/62 , A01N37/18 , C12Q1/34 , A61P31/04 , C12N9/24 , C12N15/56
CPC分类号: C12N9/2462 , A01N43/50 , A23L3/3571 , A61K38/00 , A61K38/47 , C07K7/08 , C07K14/195 , C07K14/21 , C07K14/43518 , C07K2319/21 , C07K2319/55 , C12Q1/04 , C12Q1/34 , C12Y302/01017 , G01N2333/928
摘要: The present invention relates to a polypeptide with an amino acid sequence according to SEQ ID NO: 1 and fragments or derivatives thereof. The present invention further relates to fusion proteins comprising said polypeptide and an additional peptide stretch fused to said polypeptide at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said polypeptide or fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said polypeptide or fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means, as cosmetic substance or as sanitizing agent. The present invention also relates to the use of said polypeptide or fusion protein for the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to a pharmaceutical composition comprising said polypeptide or fusion protein.
摘要翻译: 本发明涉及具有根据SEQ ID NO:1的氨基酸序列及其片段或衍生物的多肽。 本发明还涉及包含所述多肽的融合蛋白和在N-或C-末端与所述多肽融合的另外的肽拉伸。 此外,本发明涉及编码所述多肽或融合蛋白的核酸分子,包含所述核酸分子的载体和包含所述核酸分子或所述载体的宿主细胞。 此外,本发明涉及用作药物的所述多肽或融合蛋白,特别是用作治疗或预防革兰氏阴性细菌感染,作为诊断手段,作为化妆品或消毒剂。 本发明还涉及所述多肽或融合蛋白用于治疗或预防食品的食品加工设备,食品加工厂,与食品接触的表面,医疗器械的革兰氏阴性细菌污染, 在医院和手术中的表面。 此外,本发明涉及包含所述多肽或融合蛋白的药物组合物。
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公开(公告)号:US20120189606A1
公开(公告)日:2012-07-26
申请号:US13380312
申请日:2010-06-28
申请人: Rob Lavigne , Stefan Miller , Yves Briers , Guido Volckaert , Maarten Walmagh
发明人: Rob Lavigne , Stefan Miller , Yves Briers , Guido Volckaert , Maarten Walmagh
IPC分类号: A61K38/46 , C12N9/96 , C12N15/63 , A01N37/46 , C12N5/10 , C12Q1/34 , C12N1/21 , A61P31/04 , A01P1/00 , C12Q1/68 , G01N33/53 , G01N21/64 , C12N15/62 , C12N1/19
CPC分类号: A61K38/47 , A61K38/00 , A61L2/00 , C07K14/005 , C07K14/4723 , C07K2319/00 , C07K2319/20 , C12N9/2462 , C12N9/503 , C12N15/52 , C12N15/62 , C12Y302/01017 , Y02A50/475 , Y02A50/478 , Y02A50/479
摘要: The present invention relates to antimicrobial agents against Gram-negative bacteria, in particular to fusion proteins composed of an enzyme having the activity of degrading the cell wall of Gram-negative bacteria and a peptide stretch fused to the enzyme at the N- or C-terminus. Moreover, the present invention relates to nucleic acid molecules encoding said fusion protein, vectors comprising said nucleic acid molecules and host cells comprising either said nucleic acid molecules or said vectors. In addition, the present invention relates to said fusion protein for use as a medicament, in particular for the treatment or prevention of Gram-negative bacterial infections, as diagnostic means or as cosmetic substance. The present invention also relates to the treatment or prevention of Gram-negative bacterial contamination of foodstuff, of food processing equipment, of food processing plants, of surfaces coming into contact with foodstuff, of medical devices, of surfaces in hospitals and surgeries. Furthermore, the present invention relates to a pharmaceutical composition comprising said fusion protein.
摘要翻译: 本发明涉及抗革兰氏阴性细菌的抗微生物剂,特别涉及由具有降解革兰氏阴性细菌细胞壁的活性的酶组成的融合蛋白,以及与N-或C- 终点。 此外,本发明涉及编码所述融合蛋白的核酸分子,包含所述核酸分子的载体和包含所述核酸分子或所述载体的宿主细胞。 此外,本发明涉及用作药物的所述融合蛋白,特别是用于治疗或预防革兰氏阴性细菌感染,作为诊断手段或化妆品物质。 本发明还涉及食品加工设备,食品加工厂,与食品接触的表面,医疗器械,医院和手术中的表面的革兰氏阴性细菌污染的治疗或预防。 此外,本发明涉及包含所述融合蛋白的药物组合物。
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公开(公告)号:US20110306068A1
公开(公告)日:2011-12-15
申请号:US13181199
申请日:2011-07-12
申请人: Stefan Miller , Roman Meyer , Renate Grassl , Manfred Biebl , Holger Grallert
发明人: Stefan Miller , Roman Meyer , Renate Grassl , Manfred Biebl , Holger Grallert
IPC分类号: G01N33/566 , C07K1/22 , C07K14/005
CPC分类号: G01N33/566 , B01D15/08 , C07K1/13 , C07K1/22 , C07K14/005 , C12N7/00 , C12N2795/00022 , C12N2795/00031 , G01N30/00 , G01N33/56911 , G01N33/92 , G01N2333/005 , G01N2400/50
摘要: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.
摘要翻译: 本发明涉及在没有二价阳离子,特别是Ca 2+或Mg 2+的情况下能够结合内毒素的噬菌体尾蛋白及其衍生物和片段。 此外,本发明涉及使用根据本发明的噬菌体尾蛋白从溶液和样品中消耗内毒素的方法以及内毒素的检测方法。
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公开(公告)号:US08003313B2
公开(公告)日:2011-08-23
申请号:US11814358
申请日:2006-01-23
申请人: Stefan Miller , Roman Meyer , Renate Grassl , Manfred Biebl , Holger Grallert
发明人: Stefan Miller , Roman Meyer , Renate Grassl , Manfred Biebl , Holger Grallert
IPC分类号: C12Q1/70 , G01N33/569
CPC分类号: G01N33/566 , B01D15/08 , C07K1/13 , C07K1/22 , C07K14/005 , C12N7/00 , C12N2795/00022 , C12N2795/00031 , G01N30/00 , G01N33/56911 , G01N33/92 , G01N2333/005 , G01N2400/50
摘要: The present invention relates to bacteriophage tail proteins and the derivatives and fragments thereof that are capable of binding endotoxins in the absence of bivalent positive ions, especially Ca2+ or Mg2+. Further, the present invention relates to methods for depleting endotoxins from solutions and samples using the bacteriophage tail proteins according to the present invention and to a detection method for endotoxins.
摘要翻译: 本发明涉及在没有二价阳离子,特别是Ca 2+或Mg 2+的情况下能够结合内毒素的噬菌体尾蛋白及其衍生物和片段。 此外,本发明涉及使用根据本发明的噬菌体尾蛋白从溶液和样品中消耗内毒素的方法以及内毒素的检测方法。
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公开(公告)号:US20070042357A1
公开(公告)日:2007-02-22
申请号:US11445451
申请日:2006-05-31
申请人: Stefan Miller
发明人: Stefan Miller
CPC分类号: C12Q1/04 , C12Q2304/10 , G01N33/56911 , G01N33/56983 , G01N2333/924
摘要: The present invention relates to a method to detect bacteria, the method comprising the following steps: coupling of the bacteriophages and/or bacteriophage proteins to a support, incubating the support coupled with the bacteriophages and/or bacteriophage proteins with a sample, optionally removing the sample and the bacteria in the sample not bound to the bacteriophages and/or bacteriophage proteins, optionally adding substances permeabilizing or destroying the bacterial membrane, and detecting the bacteria in the sample bound to the bacteriophages and/or bacteriophage proteins, wherein the bound bacteria are not subjected to a cultivation step.
摘要翻译: 本发明涉及一种检测细菌的方法,该方法包括以下步骤:将噬菌体和/或噬菌体蛋白偶联到载体上,将与噬菌体和/或噬菌体蛋白质偶联的载体与样品一起孵育,任选地除去 样品和样品中的细菌不结合噬菌体和/或噬菌体蛋白质,任选地添加渗透或破坏细菌膜的物质,以及检测与噬菌体和/或噬菌体蛋白质结合的样品中的细菌,其中结合的细菌是 没有进行培养步骤。
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