公开(公告)号：US20120252110A1

公开(公告)日：2012-10-04

申请号：US13432658

申请日：2012-03-28

申请人： Mitsuhiro OURA ,  Teruo OKANO ,  Tatsuya SHIMIZU ,  Hirotsugu KUBO ,  Sunao TAKEDA

发明人： Mitsuhiro OURA ,  Teruo OKANO ,  Tatsuya SHIMIZU ,  Hirotsugu KUBO ,  Sunao TAKEDA

IPC分类号： C12M1/26 ,  C12M1/34

CPC分类号： C12M33/06 ,  C12M29/26 ,  C12M41/14 ,  C12M41/48

摘要： A cell culture apparatus includes: an isolator in which a sterile space accommodating a cell incubator filled with a culture solution containing cells to be cultured is disposed; a sampling unit configured to sample the culture solution in the cell incubator; a delivery flow path through which an inside of the sterile space and an outside of the sterile space communicate with each other, the delivery flow path configured to limit a flow in the delivery flow path to a direction that is directed from the inside of the sterile space toward the outside of the sterile space; and a culture solution delivering section configured to deliver the sampled culture solution to the outside of the sterile space via the delivery flow path.

摘要翻译： 细胞培养装置包括：隔离器，其中设置容纳装有包含待培养细胞的培养液的细胞培养箱的无菌空间; 取样单元，其构造为对细胞培养器中的培养液进行取样; 输送流动路径，无菌空间的内部和无菌空间的外部通过其彼此连通，输送流路被配置为将输送流动路径中的流动限制到从无菌空间的内部指向的方向 空间朝无菌空间外面; 以及培养液输送部，其构造成经由所述输送流路将取样的培养液输送到所述无菌空间的外部。

公开(公告)号：US20120214250A1

公开(公告)日：2012-08-23

申请号：US13400106

申请日：2012-02-19

申请人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

发明人： Mitsuhiro Oura ,  Teruo Okano ,  Tatsuya Shimizu ,  Hirotsugu Kubo ,  Sunao Takeda ,  Shinji Yamamori

IPC分类号： G01N21/59

CPC分类号： G01N21/80 ,  C12M41/26 ,  G01N21/3151 ,  G01N31/221 ,  G01N2201/0627 ,  G01N2201/1211

摘要： A method of measuring a pH of a solution includes: emitting light beams of two wavelengths from one side of a measuring region of a solution into which an indicator is mixed, while pulsating the solution in the measuring region; receiving at least one of transmitted light beams and reflected light beams of the emitted light beams on the other side of the measuring region, while pulsating the solution in the measuring region; obtaining absorbances of the two wavelengths based on the received at least one of the transmitted light beams and the reflected light beams; obtaining an absorbance ratio from the obtained absorbances ; and calculating a pH value of the solution based on the obtained absorbance ratio and an absorbance ratio/pH value correspondence database which is previously stored.

摘要翻译： 测量溶液的pH的方法包括：在测量区域中的溶液脉动的同时，从混合有指示剂的溶液的测量区域的一侧发射两个波长的光束; 在所述测量区域中的所述溶液脉动的同时，在测量区域的另一侧上接收发射光束的透射光束和反射光束中的至少一个; 基于所接收的所述透射光束和所述反射光束中的至少一个，获得所述两个波长的吸光度; 从所获得的吸光度获得吸光度比; 并且基于所获得的吸光度比和预先存储的吸光度比/ pH值对应数据库来计算溶液的pH值。

公开(公告)号：US20120052556A1

公开(公告)日：2012-03-01

申请号：US13214774

申请日：2011-08-22

申请人： Akane SUZUKI ,  Takahiro SHIOYAMA ,  Sunao TAKEDA

发明人： Akane SUZUKI ,  Takahiro SHIOYAMA ,  Sunao TAKEDA

IPC分类号： C12M3/00

CPC分类号： G01N1/286 ,  G01N1/38 ,  G01N15/14 ,  G01N2001/085 ,  G01N2001/2866

摘要： A cell isolation apparatus includes: a cylinder member that is inserted into a container into which a reagent is to be introduced; a net which is stretched over the cylinder member; a tissue acquiring unit that is projected from the cylinder member; and a bottom lid portion that is configured by a bottomed pipe in which a first hole that communicates an interior with an exterior is formed, and that is fitted to a bottom portion of the cylinder member in a state where the net and the tissue acquiring unit are accommodated.

摘要翻译： 细胞隔离装置包括：插入到待引入试剂的容器中的圆筒构件; 在气缸构件上拉伸的网; 组织获取单元，其从所述圆筒构件突出; 以及底盖部，其由形成有与内部连通的第一孔的有底管构成，并且在所述网和所述组织获取单元的状态下嵌合到所述筒构件的底部， 被容纳。

公开(公告)号：US20110151446A1

公开(公告)日：2011-06-23

申请号：US12702262

申请日：2010-02-08

申请人： Takahiro SHIOYAMA ,  Sunao TAKEDA ,  Akane SUZUKI ,  Naoki KOBAYASHI ,  Yuko NAGAI

发明人： Takahiro SHIOYAMA ,  Sunao TAKEDA ,  Akane SUZUKI ,  Naoki KOBAYASHI ,  Yuko NAGAI

IPC分类号： C12Q1/68

CPC分类号： C12Q1/68 ,  C12Q2563/107 ,  C12Q2565/626

摘要： A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the invention, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the invention includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye.

摘要翻译： 一种细胞处理溶液和用于制备通过流式细胞术提供给核DNA测量的染色细胞悬液的方法。 细胞处理溶液可以包括表面活性剂，核糖核酸酶和荧光染料。 表面活性剂可以包括例如非离子表面活性剂，两性离子表面活性剂，阴离子表面活性剂和/或阳离子表面活性剂。 在本发明的一种方法中，制备通过流式细胞术提供给核DNA测量的染色细胞悬浮液。 该方法可以包括将组织样品添加到细胞处理溶液中，所述细胞处理溶液包括表面活性剂，核糖核酸酶和荧光染料，分解组织样品，以及过滤分解的组织样品。 本发明的另一种方法包括分解组织样品，通过过滤分解的组织样品制备细胞悬浮液，并加入包含表面活性剂，核糖核酸酶和荧光染料的细胞处理溶液。

公开(公告)号：US20090287075A1

公开(公告)日：2009-11-19

申请号：US12407839

申请日：2009-03-20

申请人： Hiroshi KUBOTA ,  Sunao TAKEDA

发明人： Hiroshi KUBOTA ,  Sunao TAKEDA

IPC分类号： A61B5/04

CPC分类号： A61B5/0059 ,  A61B5/6833 ,  A61B2560/0412

摘要： A biological signal measurement sensor is to be fixed to a living body with an adhesive. The biological signal measurement sensor includes: a surface, which is to be in contact with a part of the living body; and a first portion, which is opposite to the surface and to which the adhesive is to be attached. At least a part of the first portion has a first patterned indented shape.

摘要翻译： 生物信号测量传感器用粘合剂固定在活体上。 生物信号测量传感器包括：与生物体的一部分接触的表面; 以及第一部分，其与表面相对并且粘合剂将附接到该第一部分。 第一部分的至少一部分具有第一图案化的缩进形状。

公开(公告)号：US06737434B2

公开(公告)日：2004-05-18

申请号：US10380828

申请日：2003-03-19

申请人： Minoru Tokizawa ,  Sunao Takeda ,  Yasushi Kaneko ,  Koji Kusano ,  Hiromichi Eto ,  Koichi Tachibana ,  Susumu Sato ,  Tadayoshi Taniyama

发明人： Minoru Tokizawa ,  Sunao Takeda ,  Yasushi Kaneko ,  Koji Kusano ,  Hiromichi Eto ,  Koichi Tachibana ,  Susumu Sato ,  Tadayoshi Taniyama

IPC分类号： C07D23360

CPC分类号： C07D233/64 ,  C07D231/12 ,  C07D233/56 ,  C07D249/08

摘要： Imidazole derivatives of the formula (1) or salts thereof, pharmaceuticals containing the derivatives or the salts, and intermediates for the synthesis of the derivatives or the salts (wherein R1 is lower alkyl; R2 is alkyl or aralkyl; and X1 is halogeno). These compounds exhibit G-CSF-like activities and can be substituted for G-CSF preparations.

摘要翻译： 式（1）的咪唑衍生物或其盐，含有衍生物或盐的药物，以及用于合成衍生物或盐的中间体（其中R 1为低级烷基; R 2为烷基或芳烷基; X 1为卤素）。 这些化合物表现出G-CSF样活性，可代替G-CSF制剂。

公开(公告)号：US06194592B1

公开(公告)日：2001-02-27

申请号：US09551569

申请日：2000-04-18

申请人： Sunao Takeda ,  Yasushi Kaneko ,  Minoru Tokizawa ,  Hiromichi Eto ,  Kazuya Ishida ,  Kazunori Maebashi ,  Masaru Matsumoto ,  Takemitsu Asaoka ,  Susumu Sato

发明人： Sunao Takeda ,  Yasushi Kaneko ,  Minoru Tokizawa ,  Hiromichi Eto ,  Kazuya Ishida ,  Kazunori Maebashi ,  Masaru Matsumoto ,  Takemitsu Asaoka ,  Susumu Sato

IPC分类号： C07D30334

CPC分类号： C07D231/12 ,  C07D233/56 ,  C07D249/08 ,  C07D303/48

摘要： Described is a triazole derivative represented by the formula (1): wherein R1 represents a hydrogen atom, a lower alkyl group or an aralkyl group, X1 and X2 are the same or different and each independently represents a hydrogen atom, a halogen atom or a halogenoalkyl and n stands for an integer of 0 to 2, or salt thereof; a preparation process of said compound and a pharmaceutical comprising said compound as an effective ingredient. The compound as described above has high antimycotic activity and is useful for the prevention and treatment of mammalian mycotic infections.

公开(公告)号：US06184396B2

公开(公告)日：2001-02-06

申请号：US09551854

申请日：2000-04-18

申请人： Sunao Takeda ,  Yasushi Kaneko ,  Minoru Tokizawa ,  Hiromichi Eto ,  Kazuya Ishida ,  Kazunori Maebashi ,  Masaru Matsumoto ,  Takemitsu Asaoka ,  Susumu Sato

发明人： Sunao Takeda ,  Yasushi Kaneko ,  Minoru Tokizawa ,  Hiromichi Eto ,  Kazuya Ishida ,  Kazunori Maebashi ,  Masaru Matsumoto ,  Takemitsu Asaoka ,  Susumu Sato

IPC分类号： C07D30334

CPC分类号： C07D231/12 ,  C07C323/22 ,  C07C2601/02 ,  C07D233/56 ,  C07D249/08 ,  C07D303/34

摘要： Described is a triazole derivative represented by the formula (1): wherein R1 represents a hydrogen atom, a lower alkyl group or an aralkyl group, X1 and X2 are the same or different and each independently represents a hydrogen atom, a halogen atom or a halogenoalkyl and n stands for an integer of 0 to 2, or salt thereof; a preparation process of said compound and a pharmaceutical comprising said compound as an effective ingredient. The compound as described above has high antimycotic activity and is useful for the prevention and treatment of mammalian mycotic infections.

公开(公告)号：US5986144A

公开(公告)日：1999-11-16

申请号：US311057

申请日：1999-05-14

申请人： Minoru Tokizawa ,  Sunao Takeda ,  Yasushi Kaneko ,  Hiromichi Eto ,  Kazuya Ishida ,  Kazunori Maebashi ,  Masaru Matsumoto ,  Takemitsu Asaoka ,  Susumu Sato ,  Hideaki Matsuda

发明人： Minoru Tokizawa ,  Sunao Takeda ,  Yasushi Kaneko ,  Hiromichi Eto ,  Kazuya Ishida ,  Kazunori Maebashi ,  Masaru Matsumoto ,  Takemitsu Asaoka ,  Susumu Sato ,  Hideaki Matsuda

IPC分类号： C07D249/08 ,  C07D303/34 ,  C07D521/00 ,  C07C323/07

CPC分类号： C07D231/12 ,  C07D233/56 ,  C07D249/08 ,  C07D303/34

摘要： Described is a triazole derivative represented by the formula (1) or salt thereof, preparation process thereof, preparation intermediates represented by the formulas (2) and (3), and a pharmaceutical comprising the compound represented by the formula (1) or salt thereof. wherein n stands for 0, 1 or 2, X.sup.1 represents H or F and Y.sup.1 and Y.sup.2 each independently represents H, halogen or CF.sub.3.

摘要翻译： 描述由式（1）表示的三唑衍生物或其盐，其制备方法，由式（2）和（3）表示的制备中间体，以及包含式（1）表示的化合物或其盐的药物 。 其中n表示0,1或2，X 1表示H或F，Y 1和Y 2各自独立地表示H，卤素或CF 3。

公开(公告)号：US5699807A

公开(公告)日：1997-12-23

申请号：US507709

申请日：1995-07-26

申请人： Jun Motogi ,  Yoshio Sakai ,  Sunao Takeda

发明人： Jun Motogi ,  Yoshio Sakai ,  Sunao Takeda

IPC分类号： A61B5/022 ,  A61B5/02

CPC分类号： A61B5/02116 ,  A61B5/022 ,  A61B5/7239

摘要： A pressure sensor gathers discrete data representing the pulse amplitude of a pulse wave signal when a cuff pressure is increased and decreased. A RAM stores the discrete data of the pulse amplitude. A CPU processes the discrete data by using a spline function, to thereby generate the data representative of a smooth continuous line passing by points of the pulse amplitude of the discrete data. This process reduces a variation of the pulse amplitude of a pulse wave signal, to thereby minimize a variation of the blood pressure values. An inflection point of the smooth continuous line is used as a diastolic blood pressure value.

摘要翻译： 当袖带压力增加和减小时，压力传感器收集表示脉搏波信号的脉冲幅度的离散数据。 RAM存储脉冲幅度的离散数据。 CPU通过使用样条函数处理离散数据，从而生成表示通过离散数据的脉冲幅度的点的平滑连续线的数据。 该过程减少了脉搏波信号的脉冲幅度的变化，从而使血压值的变化最小化。 平滑连续线的拐点被用作舒张血压值。