公开(公告)号：US20120033064A1

公开(公告)日：2012-02-09

申请号：US13205922

申请日：2011-08-09

Applicant: Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

Inventor： Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

IPC: H04N7/18

CPC classification number: H04N7/183 ,  G02B21/0004 ,  G02B21/086 ,  G02B21/26 ,  G02B21/367 ,  G02B26/008

Abstract: A microscope system includes a pigment amount calculating unit, a cell component identification processing unit, a target molecule expression portion extraction unit, a cell variant setting unit, a cell variant classification determining unit, and a display image generating unit. The pigment amount calculating unit calculates a pigment amount of a molecule target pigment for each pixel of a VS image. The cell component identification processing unit identifies a cell component based on the pigment amount. The target molecule expression portion extraction unit extracts expression portions of target molecules in the area of the cell component. The cell variant setting unit sets a cell variant including a combination of presence/absence of expressions of target molecules. The cell variant classification determining unit classifies a cell area in the VS image into a cell variant based on the combination of the expression portions of the target molecules included in the cell area.

Abstract translation: 显微镜系统包括颜料量计算单元，单元部件识别处理单元，目标分子表达部分提取单元，单元变体设置单元，单元变体分类确定单元和显示图像生成单元。 颜料量计算单元计算VS图像的每个像素的分子靶颜料的颜料量。 细胞成分识别处理单元基于颜料量鉴定细胞成分。 靶分子表达部分提取单元提取细胞成分区域中的靶分子的表达部分。 细胞变体设置单元设置包括目标分子表达的存在/不存在的组合的细胞变体。 细胞变体分类确定单元基于细胞区域中包含的靶分子的表达部分的组合，将VS图像中的细胞区域分类为细胞变体。

公开(公告)号：US07932504B2

公开(公告)日：2011-04-26

申请号：US12343748

申请日：2008-12-24

Applicant: Tatsuki Yamada

Inventor： Tatsuki Yamada

IPC: G01N21/64

CPC classification number: G02B21/16 ,  G01N21/6458 ,  G02B21/18

Abstract: A whole specimen image generated in the bright view field observation method is displayed, and arbitrary domains are designated. And the fluorescence VS image of each object of interest is generated by acquiring the shape of microscopic image for every small block which is formed by dividing the inside of that object of interest into plurality for every said object of interest, and combining two or more microscopic images generated in the fluorescence observation method. The object of interest is an area where a tumor etc., exist, for example.

Abstract translation: 显示在亮视野观察方法中产生的整体标本图像，并指定任意域。 并且通过对于每个所述感兴趣对象将所述感兴趣对象的内部分成多个而形成的每个小块获取显微图像的形状，并且组合两个或更多个微观的 在荧光观察方法中产生的图像。 感兴趣的对象是例如存在肿瘤等的区域。

公开(公告)号：US20100141752A1

公开(公告)日：2010-06-10

申请号：US12629547

申请日：2009-12-02

Applicant: Tatsuki Yamada ,  Shinsuke Tani ,  Takeshi Otsuka ,  Satoshi Arai ,  Yuichi Ishikawa ,  Kengo Takeuchi

Inventor： Tatsuki Yamada ,  Shinsuke Tani ,  Takeshi Otsuka ,  Satoshi Arai ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC: G09G5/02 ,  H04N7/18 ,  G09G5/00

CPC classification number: G01N1/312 ,  G01N2035/00039 ,  G01N2035/00138 ,  G02B21/26 ,  G02B21/367

Abstract: A microscope system includes an image acquiring unit that acquires a specimen image formed by capturing a specimen multi-stained by a plurality of pigments using a microscope; a pigment amount acquiring unit that acquires a pigment amount of each pigment staining a corresponding position on the specimen, for each pixel of the specimen image; and a pigment selecting unit that selects a display target pigment from the plurality of pigments. The system also includes a display image generating unit that generates a display image where a staining state of the specimen by the display target pigment is displayed, on the basis of the pigment amount of the display target pigment in each pixel of the specimen image; and a display processing unit that displays the display image on a display unit.

Abstract translation: 显微镜系统包括图像获取单元，其获取通过使用显微镜捕获多个颜料多染色的样本而形成的标本图像; 颜料量获取单元，针对样本图像的每个像素获取每个颜料的颜料量染色样品上的相应位置; 以及颜料选择单元，其从多种颜料中选择显示目标颜料。 该系统还包括显示图像生成单元，其基于样本图像的每个像素中的显示目标颜料的颜料量，生成显示目标颜料的样品的染色状态的显示图像; 以及显示处理单元，其将显示图像显示在显示单元上。

公开(公告)号：US20100322502A1

公开(公告)日：2010-12-23

申请号：US12816472

申请日：2010-06-16

Applicant: Takeshi OTSUKA ,  Tatsuki YAMADA ,  Yuichi ISHIKAWA ,  Kengo TAKEUCHI

Inventor： Takeshi OTSUKA ,  Tatsuki YAMADA ,  Yuichi ISHIKAWA ,  Kengo TAKEUCHI

IPC: G06K9/00

CPC classification number: G06K9/0014 ,  G06T7/0012 ,  G06T2207/10024 ,  G06T2207/10056 ,  G06T2207/30024

Abstract: The present invention provides a medical diagnosis support device, which is capable of acquiring information to support medical diagnosis for easily and precisely determining chromosome abnormality and/or gene amplification related to cancer or genetic disorder.The medical diagnosis support device for acquiring information to support medical diagnosis from an image of a specimen stained by multiple staining, the image is obtained by photographing the stained specimen with transmitted light, the device comprises: staining characteristics quantity acquisition means for acquiring characteristics quantity of each staining, based on a pixel value of the image of the stained specimen; marker intensifying means for intensifying a marker, based on the characteristics quantity of each staining thus acquired; marker extracting means for extracting the marker of each staining, based on the characteristics quantity in which the marker has been thus intensified; marker state judging means for judging a state of the marker, based on the marker of each staining thus extracted; and marker state identifying and displaying means for identifying and displaying the marker state, based on the judgment result.

Abstract translation: 本发明提供一种能够获取信息以支持医学诊断以容易且精确地确定与癌症或遗传疾病相关的染色体异常和/或基因扩增的医学诊断支持装置。 用于从通过多次染色染色的样本的图像获取用于支持医学诊断的信息的医疗诊断支持装置，通过用透射光拍摄染色的样本获得图像，该装置包括：用于获取特征量的染色特征量获取装置 每次染色，基于染色标本的图像的像素值; 基于如此获得的每个染色的特征量来增强标记物的标记增强装置; 标记提取装置，用于基于标记已被强化的特征量提取每个染色的标记物; 标记状态判断装置，用于基于如此提取的每个染色的标记来判断标记的状态; 以及标记状态识别和显​​示装置，用于基于判断结果来识别和显示标记状态。

公开(公告)号：US06259080B1

公开(公告)日：2001-07-10

申请号：US09268587

申请日：1999-03-15

Applicant: Zheng Li ,  Takashi Yoneyama ,  Tatsuki Yamada ,  Takashi Nagano

Inventor： Zheng Li ,  Takashi Yoneyama ,  Tatsuki Yamada ,  Takashi Nagano

IPC: G02B704

CPC classification number: G02B21/244

Abstract: The present invention is an automatic focal point detection device. In a broad visual field and high resolution observation, when focusing is impossible in a splitting visual field obtained by splitting a specimen in a visual field in plural number, a stage is moved to an in-focus position stored in advance and focusing is executed. Besides the device is provided with an excitation light shutter for intercepting an excitation light with which a specimen is irradiated and when stray light corresponding to a difference detected by closing and opening the shutter in fluorescence observation is smaller than a specific value, specimen search is performed and focusing is executed at a level exceeding stray light, while the difference is equal to or larger than the specific value, focusing is executed with nothing more done.

Abstract translation: 本发明是一种自动焦点检测装置。 在宽视野和高分辨率观察中，当通过在多个视野中分割样本而获得的分割视野中不可能进行聚焦时，将舞台移动到预先存储的对焦位置并进行聚焦。 此外，该装置设置有用于截取照射样本的激发光的激发光快门，并且当在荧光观察中对应于通过关闭和打开快门检测到的差异的杂散光小于特定值时，进行样本搜索 并且在超过杂散光的水平上执行聚焦，而差异等于或大于特定值，则不再进行聚焦。

公开(公告)号：US08937653B2

公开(公告)日：2015-01-20

申请号：US13205922

申请日：2011-08-09

Applicant: Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

Inventor： Tatsuki Yamada ,  Futoshi Akiyama ,  Rie Horii

IPC: H04N7/18 ,  G02B21/00 ,  G02B21/36 ,  G02B26/00 ,  G02B21/08 ,  G02B21/26

CPC classification number: H04N7/183 ,  G02B21/0004 ,  G02B21/086 ,  G02B21/26 ,  G02B21/367 ,  G02B26/008

Abstract: A microscope system includes a pigment amount calculating unit, a cell component identification processing unit, a target molecule expression portion extraction unit, a cell variant setting unit, a cell variant classification determining unit, and a display image generating unit. The pigment amount calculating unit calculates a pigment amount of a molecule target pigment for each pixel of a VS image. The cell component identification processing unit identifies a cell component based on the pigment amount. The target molecule expression portion extraction unit extracts expression portions of target molecules in the area of the cell component. The cell variant setting unit sets a cell variant including a combination of presence/absence of expressions of target molecules. The cell variant classification determining unit classifies a cell area in the VS image into a cell variant based on the combination of the expression portions of the target molecules included in the cell area.

Abstract translation: 显微镜系统包括颜料量计算单元，单元部件识别处理单元，目标分子表达部分提取单元，单元变体设置单元，单元变体分类确定单元和显示图像生成单元。 颜料量计算单元计算VS图像的每个像素的分子靶颜料的颜料量。 细胞成分识别处理单元基于颜料量鉴定细胞成分。 靶分子表达部分提取单元提取细胞成分区域中的靶分子的表达部分。 细胞变体设置单元设置包括目标分子表达的存在/不存在的组合的细胞变体。 细胞变体分类确定单元基于细胞区域中包含的靶分子的表达部分的组合，将VS图像中的细胞区域分类为细胞变体。

公开(公告)号：US08350905B2

公开(公告)日：2013-01-08

申请号：US12357059

申请日：2009-01-21

Applicant: Tatsuki Yamada

Inventor： Tatsuki Yamada

IPC: H04N7/18 ,  G06K9/00

CPC classification number: G01N21/6458 ,  G02B21/367 ,  G06K9/0014 ,  G06T5/50 ,  G06T7/0012 ,  G06T2200/04 ,  G06T2207/10016 ,  G06T2207/10056 ,  G06T2207/10148 ,  G06T2207/30024

Abstract: A microscope system has a VS image generation means for generating a virtual slide image of a specimen which is constructed by mutually connecting a plurality of microscope images with a first photomagnification photographed and acquired whenever an objective lens and the specimen are relatively moved in a direction perpendicular to the optical axis and which represents the entire image of the specimen, an object-of-interest set means setting an object of interest with respect to the entire image of the specimen represented by the VS image, and a three-dimensional VS image generation means for generating a three-dimensional VS image which is constructed by connecting the microscope images at different focal positions in accordance with the same focal position and which is constructed from the microscope images with a second photomagnification higher than the first photomagnification and represents the image of the object of interest.

Abstract translation: 显微镜系统具有用于产生样本的虚拟幻灯片图像的VS图像产生装置，每当物镜和样本在垂直方向上相对移动时，通过相互连接多个显微镜图像和第一次光学曝光而获得的样本 相对于由VS图像表示的样本的整个图像设置感兴趣对象的三维VS图像生成装置，其特征在于， 用于产生三维VS图像的装置，其通过根据相同焦点位置连接不同焦点位置处的显微镜图像而构成，并且由具有高于第一光放大的第二光放大的显微镜图像构成，并且表示 感兴趣的对象。

公开(公告)号：US20110212486A1

公开(公告)日：2011-09-01

申请号：US12950268

申请日：2010-11-19

Applicant: Tatsuki Yamada ,  Yuichi Ishikawa ,  Kengo Takeuchi

Inventor： Tatsuki Yamada ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC: G01N33/48 ,  C12M1/34

CPC classification number: G02B21/365 ,  G01N1/30

Abstract: A microscope system includes an acquisition unit that obtains a specimen image acquired by capturing the specimen stained by an element identification dye that visualizes a predetermined cell constituent element and a molecule target dye that visualizes a predetermined target molecule by using a microscope; a dye amount unit that obtains dye amounts of the element identification dye and the molecule target dye that stain corresponding positions on the specimen for each pixel of the image; an element area identification unit that identifies an area of the cell constituent element based on the dye amount of the element identification dye; a condition setting unit that sets the presence or absence of the predetermined target molecule on the cell constituent element as a condition; and an extraction unit that extracts an area of a target portion that satisfies the condition based on the dye amount of the molecule target dye.

Abstract translation: 显微镜系统包括获取单元，其获取通过捕获通过使用显微镜可视化预定细胞构成元素的元素识别染料和可视化预定靶分子的分子靶染料而染色的样品而获得的标本图像; 染色量单元，其获得染色剂量，所述染料量针对图像的每个像素染色样品上的相应位置的元件识别染料和分子靶染料; 元件区域识别单元，其基于元件识别染料的染色量来识别单元构成元件的面积; 条件设定单元，其将所述细胞构成元件上的所述规定的目标分子的存在或不存在作为条件; 以及提取单元，其基于分子靶染料的染料量提取满足条件的目标部分的面积。

公开(公告)号：US07865007B2

公开(公告)日：2011-01-04

申请号：US11589529

申请日：2006-10-30

Applicant: Tatsuki Yamada

Inventor： Tatsuki Yamada

IPC: G06K9/20 ,  G06K9/36

CPC classification number: G02B21/36

Abstract: It is possible to provide a microscope system, capable of constructing a wide vision and high definition microscope image without requiring a work by a pathologist, of reducing a storage capacity for recording and storing after a pathologist observing and/or determining a diagnosis, and of forming and displaying a wide vision and high definition microscope image by comprising means for obtaining image information of the entirety, or a part, of a sample by moving an object lens and a sample relatively to each other in the perpendicular direction against an optical axis, means for designating a specific zone of the obtained image information, means for storing image information of the designated specific zone, means for reducing an information volume of image information not designated among the obtained image information, means for storing the reduced image information, and means for storing a positional relationship between these pieces of stored image information.

Abstract translation: 可以提供一种显微镜系统，能够构建宽视野和高分辨率显微镜图像，而不需要病理学家的工作，在病理学家观察和/或确定诊断之后降低用于记录和存储的存储容量，以及 通过包括通过相对于光轴在垂直方向上相对移动物镜和样本来获取样本的整体或部分的图像信息的装置来形成和显示宽视力和高清晰度显微镜图像， 用于指定所获得的图像信息的特定区域的装置，用于存储所指定的特定区域的图像信息的装置，用于减少所获得的图像信息中未指定的图像信息的信息量的装置，用于存储缩小的图像信息的装置， 用于存储这些存储的图像信息之间的位置关系。

公开(公告)号：US20100272334A1

公开(公告)日：2010-10-28

申请号：US12817451

申请日：2010-06-17

Applicant: Tatsuki Yamada ,  Satoshi Arai ,  Yoko Yamamoto ,  Yuichi Ishikawa ,  Kengo Takeuchi

Inventor： Tatsuki Yamada ,  Satoshi Arai ,  Yoko Yamamoto ,  Yuichi Ishikawa ,  Kengo Takeuchi

IPC: G06T7/00

CPC classification number: G01N1/312 ,  G01N2035/00039 ,  G01N2035/00138 ,  G02B21/26 ,  G02B21/367

Abstract: A microscope system includes an image acquisition unit for acquiring a spectral image of a specimen by using a microscope, a structure specifying unit for specifying an extraction target structure in the specimen, a display method specifying unit for specifying a display method of the extraction target structure, a structure extraction unit for extracting an area of the extraction target structure in the spectral image by using a reference spectrum of the extraction target structure on the basis of pixel values of each pixel included in the spectral image, a display image generator for generating a display image that represents the extraction target structure in the specimen by the display method specified by the display method specifying unit on the basis of an extraction result of the structure extraction unit, and a display processing unit for performing process for displaying the display image on a display unit.

Abstract translation: 显微镜系统包括用于通过使用显微镜获取样本的光谱图像的图像获取单元，用于指定样本中的提取目标结构的结构指定单元，用于指定提取目标结构的显示方法的显示方法确定单元 一种结构提取单元，用于根据包括在所述分光图像中的每个像素的像素值，通过使用所述提取目标结构的参考光谱来提取所述光谱图像中的所述提取目标结构的区域;显示图像生成器， 显示图像，其通过基于结构提取单元的提取结果的显示方法指定单元指定的显示方法来显示样本中的提取目标结构;以及显示处理单元，用于执行将显示图像显示在 显示单元。