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公开(公告)号：US08628785B2
公开(公告)日：2014-01-14
申请号：US13163018
申请日：2011-06-17
申请人： Ching-Liang Chu , Tzu-Chih Chen
发明人： Ching-Liang Chu , Tzu-Chih Chen
CPC分类号： A61K39/39 , A61K39/0011 , A61K2039/5154 , A61K2039/53 , A61K2039/55516 , A61K2039/572 , C12N5/0639 , C12N2501/998
摘要： The present invention relates to a method for augmenting the immunogenicity of an antigen in a mammal, comprising immunizing the mammal with a composition comprising the antigen, and an adjuvant in an amount of effective to augment the immunogenicity of said antigen, wherein the adjuvant comprises a Ling-Zhi-8 (LZ-8) protein.
摘要翻译： 本发明涉及一种增强哺乳动物抗原免疫原性的方法，包括用包含该抗原的组合物免疫哺乳动物和一种有效增加所述抗原的免疫原性的佐剂，其中佐剂包含 灵芝8（LZ-8）蛋白。
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2.发明授权Fungal immunomodulatory protein (FIP) prepared by microorganisms and uses thereof 有权通过微生物制备的真菌免疫调节蛋白（FIP）及其用途公开(公告)号：US08163519B2
公开(公告)日：2012-04-24
申请号：US10572563
申请日：2004-09-14
申请人： Jiunn-Liang Ko , Yu-Lu Huang , Tzu-Chih Chen , Hsu-Wei Hung , Ho-Lung Jiang , Ching-Lung Hu , Cheng-Chun Kuan , Hsuan-Ju Thou
发明人： Jiunn-Liang Ko , Yu-Lu Huang , Tzu-Chih Chen , Hsu-Wei Hung , Ho-Lung Jiang , Ching-Lung Hu , Cheng-Chun Kuan , Hsuan-Ju Thou
CPC分类号： C07K14/37 , A61K36/064 , A61K38/00
摘要： The invention relates to an improved nucleic acid molecule encoding fungal immunomodulatory protein (FIP) that is better expressed in fungi, to vectors comprising the nucleic acid molecule, to hosts transformed with said vectors, to processes of expressing the protein of the invention in said transformed hosts, to the protein of the invention produced by said processes, to uses of said hosts comprising the protein of the invention and to a process of purifying FIP. The protein of the invention has wide immunomodulatory activity. Thus, the present invention further relates to uses of the protein of the invention in cosmetic or pharmaceutical compositions and to food or feed additives comprising the protein of the invention. Finally, the invention relates to the method of modulating immunological activities by orally administering FIP or proteins fused with FIP to a subject.
摘要翻译： 本发明涉及一种编码真菌免疫调节蛋白（FIP）的改良的核酸分子，其在真菌中更好地表达，包含核酸分子的载体，用所述载体转化的宿主，在所述转化的表达本发明的蛋白质的过程中 宿主，由所述方法产生的本发明的蛋白质，涉及包含本发明蛋白质的所述宿主的用途以及纯化FIP的方法。 本发明的蛋白质具有广泛的免疫调节活性。 因此，本发明还涉及本发明的蛋白质在化妆品或药物组合物中的用途，以及包含本发明蛋白质的食品或饲料添加剂的用途。 最后，本发明涉及通过口服FIP或与FIP融合的蛋白质来调节免疫活性的方法。
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公开(公告)号：US07098033B2
公开(公告)日：2006-08-29
申请号：US11043739
申请日：2005-01-26
申请人： Tzu-Chih Chen , Wei-Ni Hua
发明人： Tzu-Chih Chen , Wei-Ni Hua
IPC分类号： C12N15/64
CPC分类号： C12N15/87
摘要： A fast method of transforming competent cells is described. The competent cells are thawed at room temperature or in a water bath. Plasmid DNAs and competent cells are mixed together, then the mixture is subject to heat shock treatment. After plating the mixture on a low-temperature selective medium by a low-temperature plating tool, the competent cells are cultured on the selective medium.
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4.发明授权公开(公告)号：US07087739B2
公开(公告)日：2006-08-08
申请号：US10737482
申请日：2003-12-16
申请人： Shuai-Jan Hu , Tzu-Chih Chen
发明人： Shuai-Jan Hu , Tzu-Chih Chen
CPC分类号： C07K14/585 , C07H21/04
摘要： Nucleic acid encoding recombinant salmon calcitonin, expression vector thereof, and method for producing recombinant salmon calcitonin therewith. The nucleic acid encoding recombinant salmon calcitonin comprises the sequence of SEQ ID No. 2.
摘要翻译： 编码重组鲑鱼降钙素的核酸，其表达载体及其生产重组鲑降钙素的方法。 编码重组鲑鱼降钙素的核酸包含SEQ ID No.2的序列。
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5.发明申请公开(公告)号：US20050266537A1
公开(公告)日：2005-12-01
申请号：US11123115
申请日：2005-05-06
申请人： Tzu-Chih Chen
发明人： Tzu-Chih Chen
IPC分类号： A23D9/00 , A23K1/00 , A23K1/16 , A23L1/29 , A23L1/30 , A61K8/36 , A61K8/67 , A61Q19/00 , C11B1/06 , C12N1/18 , C12N1/21 , C12N9/02 , C12N9/04 , C12N9/10 , C12P7/64
CPC分类号： A61K8/361 , A23D9/00 , A23K20/158 , A23L33/12 , A23L33/14 , A23V2002/00 , A61K8/67 , A61K2800/85 , A61Q19/00 , C11B1/06 , C12N9/0083 , C12N9/1029 , C12P7/6409 , C12P7/6427 , C12P7/6463 , C12P7/6472 , A23V2250/1882 , A23V2250/218 , A23V2250/188 , A23V2250/1872 , A23V2250/1874 , A23V2250/1862 , A23V2250/187 , A23V2250/1868
摘要： The present invention is directed to a process of producing novel fatty acids in oleaginous yeast by producing oleaginous yeast by introducing into the yeast genes coding for enzymes selected from the group consisting of D5-desaturase, D6-desaturase, D12-desaturase, D15-desaturase and elongase; and culturing the yeast in the medium containing high levels of carbon sources. The present invention is further directed to a residue or fatty acid that is obtained from pressing the oleaginous yeast produced by the process of the invention.
摘要翻译： 本发明涉及一种通过向编码选自D5-去饱和酶，D6-去饱和酶，D12-去饱和酶，D15-去饱和酶 和延伸酶; 并在含有高含量碳源的培养基中培养酵母。 本发明还涉及通过压榨由本发明的方法生产的含油酵母获得的残余物或脂肪酸。
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6.发明授权公开(公告)号：US08629096B2
公开(公告)日：2014-01-14
申请号：US13422789
申请日：2012-03-16
申请人： Jiunn-Liang Ko , Tzu-Chih Chen
发明人： Jiunn-Liang Ko , Tzu-Chih Chen
CPC分类号： A61K38/16 , C07K14/375
摘要： This invention relates to a method for stimulation or an activation of immunological function directed to activate natural killer cells and macrophages or increase production of serum antibody in a patient in need of such stimulation or activation, comprising administering an isolated and/or purified polypeptide of a fungal immunomodulatory protein. This invention also relates to a method for suppressing proliferation of a cancer cell and a method for suppressing a tumor cell mobility, comprising providing to the tumor cell a purified polypeptide of a fungal immunomodulatory protein.
摘要翻译： 本发明涉及一种用于刺激或激活旨在激活天然杀伤细胞和巨噬细胞或增加需要这种刺激或激活的患者中的血清抗体产生的免疫功能的方法，包括施用分离和/或纯化的多肽 真菌免疫调节蛋白。 本发明还涉及一种抑制癌细胞增殖的方法和抑制肿瘤细胞迁移率的方法，包括向肿瘤细胞提供纯化的真菌免疫调节蛋白多肽。
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7.发明申请公开(公告)号：US20120177674A1
公开(公告)日：2012-07-12
申请号：US13422789
申请日：2012-03-16
申请人： Jiunn-Liang Ko , Tzu-Chih Chen
发明人： Jiunn-Liang Ko , Tzu-Chih Chen
CPC分类号： A61K38/16 , C07K14/375
摘要： This invention relates to a method for stimulation or an activation of immunological function directed to activate natural killer cells and macrophages or increase production of serum antibody in a patient in need of such stimulation or activation, comprising administering an isolated and/or purified polypeptide of a fungal immunomodulatory protein. This invention also relates to a method for suppressing proliferation of a cancer cell and a method for suppressing a tumor cell mobility, comprising providing to the tumor cell a purified polypeptide of a fungal immunomodulatory protein.
摘要翻译： 本发明涉及一种用于刺激或激活旨在激活天然杀伤细胞和巨噬细胞或增加需要这种刺激或激活的患者中的血清抗体产生的免疫功能的方法，包括施用分离和/或纯化的多肽 真菌免疫调节蛋白。 本发明还涉及一种抑制癌细胞增殖的方法和抑制肿瘤细胞迁移率的方法，包括向肿瘤细胞提供纯化的真菌免疫调节蛋白多肽。
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公开(公告)号：US07820443B2
公开(公告)日：2010-10-26
申请号：US11370179
申请日：2006-03-08
申请人： Tzu-Chih Chen
发明人： Tzu-Chih Chen
CPC分类号： C12N15/87
摘要： The invention relates to a fast method of transforming competent cells, comprising: a. mixing a plasmid DNA with the competent cells suspending within ionic solution to form a mixture; b. plating the mixture on a warm selective medium; and c. culturing the mixture on the medium; wherein the ionic solution comprises divalent cation selected from the group consisting of Ca2+, Mg2+, Mn2+, Zn2+, Cu2+, Cd2+, Fe2+, Sr2+ and Co2+, and provided that the ionic solution does not include Ca2+ alone.
摘要翻译： 本发明涉及一种转化感受态细胞的快速方法，包括：a。 将质粒DNA与悬浮在离子溶液中的感受态细胞混合以形成混合物; b。 将混合物在温热的选择性介质上电镀; 和c。 在培养基上培养混合物; 其中所述离子溶液包含选自由Ca2 +，Mg2 +，Mn2 +，Zn2 +，Cu2 +，Cd2 +，Fe2 +，Sr2 +和Co2 +组成的组的二价阳离子，并且条件是离子溶液不包括单独的Ca 2+。
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9.发明申请公开(公告)号：US20100009915A1
公开(公告)日：2010-01-14
申请号：US12497898
申请日：2009-07-06
申请人： JIUNN-LIANG KO , TZU-CHIH CHEN
发明人： JIUNN-LIANG KO , TZU-CHIH CHEN
CPC分类号： A61K38/16 , C07K14/375
摘要： This invention relates to a method for stimulation or an activation of immunological function directed to activate natural killer cells and macrophages or increase production of serum antibody in a patient in need of such stimulation or activation, comprising administering an isolated and/or purified polypeptide of a fungal immunomodulatory protein. This invention also relates to a method for suppressing proliferation of a cancer cell and a method for suppressing a tumor cell mobility, comprising providing to the tumor cell a purified polypeptide of a fungal immunomodulatory protein.
摘要翻译： 本发明涉及一种用于刺激或激活旨在激活天然杀伤细胞和巨噬细胞或增加需要这种刺激或激活的患者中的血清抗体产生的免疫功能的方法，包括施用分离和/或纯化的多肽 真菌免疫调节蛋白。 本发明还涉及一种抑制癌细胞增殖的方法和抑制肿瘤细胞迁移率的方法，包括向肿瘤细胞提供纯化的真菌免疫调节蛋白多肽。
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公开(公告)号：US20050130289A1
公开(公告)日：2005-06-16
申请号：US11043739
申请日：2005-01-26
申请人： Tzu-Chih Chen , Wei-Ni Hua
发明人： Tzu-Chih Chen , Wei-Ni Hua
IPC分类号： C12N15/09 , C12M1/00 , C12M1/02 , C12M1/26 , C12N1/21 , C12N15/64 , C12N15/70 , C12N15/74 , C12N15/87 , C12R1/19
CPC分类号： C12N15/87
摘要： A fast method of transforming competent cells is described. The competent cells are thawed at room temperature or in a water bath. Plasmid DNAs and competent cells are mixed together, then the mixture is subject to heat shock treatment. After plating the mixture on a low-temperature selective medium by a low-temperature plating tool, the competent cells are cultured on the selective medium.
摘要翻译： 描述了转化感受态细胞的快速方法。 感受态细胞在室温或水浴中解冻。 将质粒DNA和感受态细胞混合在一起，然后对该混合物进行热休克处理。 通过低温电镀工具在低温选择性培养基上电镀该混合物后，感受态细胞在选择性培养基上培养。
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