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36 条记录 (耗时 0.037 秒)

    A DEVICE FOR ASEPTICALLY TREATING A SUSPENSION
    4.
    发明申请
    A DEVICE FOR ASEPTICALLY TREATING A SUSPENSION 审中-公开

    公开(公告)号：US20190194597A1

    公开(公告)日：2019-06-27

    申请号：US16331679

    申请日：2017-09-25

    申请人： Yoshikazu Yonemitsu ,  GAIA BioMedicine Inc.

    发明人： Yui Harada ,  Yoshikazu Yonemitsu

    IPC分类号： C12M1/00 ,  B01D63/02 ,  B01L3/00

    CPC分类号： C12M47/04 ,  A61K35/15 ,  A61K35/17 ,  A61P37/04 ,  B01D63/02 ,  B01L3/561 ,  B01L3/567 ,  C12M1/12 ,  C12M1/26 ,  C12N1/02

    摘要： There is provided a device comprising at least the followings: a hollow fiber type separator for separating a liquid A from a suspension comprising an inlet and outlet for passing the suspension, which communicate with the inside of hollow fibers, and at least one discharge port for discharging the separated liquid A, which communicates with the outside of the hollow fibers; an aseptic connection connector for aseptically connecting a bag containing the suspension in a non-aseptic environment, which is connected to the inlet of the hollow fiber type separator; a drainage bag for storing the separated liquid A, which is connected to the discharge port of the hollow fiber type separator; and a collection bag for storing a suspensoid from which the liquid A has been separated, which is connected to the inlet or outlet of the hollow fiber type separator.

    Method for amplifying NK cells
    5.
    发明授权
    Method for amplifying NK cells 有权
    NK细胞扩增方法

    公开(公告)号：US09404083B2

    公开(公告)日：2016-08-02

    申请号：US14129143

    申请日：2012-06-20

    申请人： Yoshikazu Yonemitsu ,  Yui Harada ,  Satoru Saito ,  Yuichiro Yazaki ,  Masato Okamoto ,  Takefumi Ishidao

    发明人： Yoshikazu Yonemitsu ,  Yui Harada ,  Satoru Saito ,  Yuichiro Yazaki ,  Masato Okamoto ,  Takefumi Ishidao

    IPC分类号： A61K35/28 ,  A61K38/00 ,  C12N5/0783 ,  A61K35/17

    CPC分类号： C12N5/0646 ,  A61K35/17 ,  C12N2501/2302

    摘要： A technique is needed which can amplify NK cells in vitro and prepare optimum number of NK cells for the adoptive immunotherapy.A method for amplifying NK cells is provided which comprises steps of: preparing cell population which is comprised of NK cells, removing T cells from the cell population which is comprised of NK cells, and, after removal of T cells, cultivating the remaining cells in a medium supplemented with 2500 to 2831 IU/mL of IL-2. The method for amplifying NK cells of the present invention may comprise a step of removing hematopoietic progenitor cells from the cell population. The present invention provides a pharmaceutical composition for adoptive immunotherapy, comprising NK cells which are prepared by the amplifying method of the present invention.

    摘要翻译： 需要一种可以在体外扩增NK细胞并为过继性免疫治疗准备最佳数量的NK细胞的技术。 提供了扩增NK细胞的方法，其包括以下步骤：制备由NK细胞组成的细胞群，从由NK细胞组成的细胞群中除去T细胞，并且在除去T细胞后，将剩余细胞培养 补充有2500至2831IU / mL的IL-2的培养基。 用于扩增本发明的NK细胞的方法可以包括从细胞群中除去造血祖细胞的步骤。 本发明提供了一种用于过继免疫治疗的药物组合物，其包含通过本发明的放大方法制备的NK细胞。

    Method for producing dendritic cells
    6.
    发明授权
    Method for producing dendritic cells 有权
    树突状细胞的制备方法

    公开(公告)号：US08741639B2

    公开(公告)日：2014-06-03

    申请号：US13127753

    申请日：2009-11-13

    申请人： Yoshikazu Yonemitsu ,  Yasuji Ueda ,  Yui Harada

    发明人： Yoshikazu Yonemitsu ,  Yasuji Ueda ,  Yui Harada

    IPC分类号： A61K39/00 ,  C12N5/0784

    CPC分类号： C12N5/0639 ,  A61K39/00 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/22 ,  C12N2501/23 ,  C12N2501/26

    摘要： An objective of the present invention is to provide methods for producing dendritic cells (DCs), which comprise the step of culturing DC precursor cells in the presence of a plurality of cytokines, produced dendritic cells, and uses thereof.The present inventors discovered that dendritic cells with a high IL-12 productivity can be obtained by culturing DC precursor cells in the presence of a plurality of cytokines, followed by about one week of culture in the presence of GM-CSF and IL-4. The present invention enables preparation of a large amount of DCs with a high IL-12 productivity from a small number of DC precursor cells, and therefore makes it easier to increase the number of DCs for administration in DC-based anti-tumor immune therapy, treatment of infections, etc. Thus, the effect of DC vaccines is expected to be enhanced.

    摘要翻译： 本发明的目的是提供用于制备树突状细胞（DC）的方法，其包括在多种细胞因子，产生的树突状细胞的存在下培养DC前体细胞的步骤及其应用。 本发明人发现通过在多种细胞因子的存在下培养DC前体细胞，然后在GM-CSF和IL-4存在下培养约1周，可获得具有高IL-12生产能力的树突状细胞。 本发明能够从少量的DC前体细胞制备大量具有高IL-12生产能力的DC，因此可以更容易地增加DC-抗肿瘤免疫治疗中用于给药的DC数量， 治疗感染等。因此，预期DC疫苗的效果将得到提高。

    Methods For Suppressing Tumor Proliferation
    9.
    发明申请
    Methods For Suppressing Tumor Proliferation 审中-公开
    抑制肿瘤增生的方法

    公开(公告)号：US20080199438A1

    公开(公告)日：2008-08-21

    申请号：US10598947

    申请日：2005-03-15

    申请人： Katsuo Sueishi ,  Yoshikazu Yonemitsu ,  Yasunori Shikada ,  Norifumi Tsutsumi ,  Mamoru Hasegawa

    发明人： Katsuo Sueishi ,  Yoshikazu Yonemitsu ,  Yasunori Shikada ,  Norifumi Tsutsumi ,  Mamoru Hasegawa

    IPC分类号： A61K48/00 ,  A61K31/7105 ,  A61K38/02 ,  A61P35/00

    CPC分类号： A61K38/179 ,  C12N15/1136 ,  C12N2310/11 ,  C12N2310/14

    摘要： The present invention provides methods for suppressing tumor proliferation comprising the step of inhibiting the expression of PDGF-A or the binding between PDGF-A homodimers and PDGFRα. Activation of the PDGFRα-p70S6K signal transduction pathway by PDGF-AA is an important factor in tumor angiogenesis and relates to the prognosis of patients suffering from tumors. By inhibiting PDGF-A expression in tumors or in their surrounding tissues, or by inhibiting the binding between PDGF-A homodimers and PDGFRα, the formation and retention of tumor vasculature can be inhibited, thereby suppressing tumor proliferation.

    摘要翻译： 本发明提供了抑制肿瘤增殖的方法，包括抑制PDGF-A的表达或PDGF-A同源二聚体与PDGFRα之间的结合的步骤。 通过PDGF-AA激活PDGFRα-p70S6K信号转导途径是肿瘤血管生成中的重要因素，涉及肿瘤患者的预后。 通过抑制肿瘤或其周围组织中的PDGF-A表达，或通过抑制PDGF-A同源二聚体和PDGFRα之间的结合，可以抑制肿瘤血管的形成和保留，从而抑制肿瘤的增殖。