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1.发明授权Therapeutic agents for diseases associated with apoptotic degeneration in ocular tissue cells that use SIV-PEDF vectors 有权与使用SIV-PEDF载体的眼组织细胞凋亡变性相关疾病的治疗剂公开(公告)号：US08278284B2
公开(公告)日：2012-10-02
申请号：US11884738
申请日：2006-02-21
申请人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
发明人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
CPC分类号： A61K48/005 , A61K48/00 , C07K14/475 , C12N15/86 , C12N2740/15043 , C12N2740/15045 , C12N2810/6081
摘要： The present invention provides novel methods for treating diseases associated with apoptotic degeneration in ocular tissue cells by effective administration of pigment epithelium derived factor (PEDF). The present inventors studied PEDF as a means to prevent ganglion cell death, the final pathology of glaucoma. The present invention is particularly focused on SIV vectors for effective methods for delivering PEDF, and constructed an SIV-PEDF vector. When the SIV-PEDF vector was administered subretinally to an ischemia reperfusion model and NMDA-induced model, a significant suppression effect on ganglion cell death was observed. The present inventors therefore discovered that the SIV-PEDF vector is an effective pharmaceutical agent for treating diseases associated with apoptotic degeneration in ocular tissue cells, such as glaucoma.
摘要翻译： 本发明提供了通过有效施用色素上皮衍生因子（PEDF）来治疗与眼组织细胞凋亡变性相关的疾病的新方法。 本发明人研究了PEDF作为预防神经节细胞死亡，青光眼的最终病理学的手段。 本发明特别关注用于递送PEDF的有效方法的SIV载体，并构建了SIV-PEDF载体。 当将SIV-PEDF载体视觉上给予缺血再灌注模型和NMDA诱导的模型时，观察到对神经节细胞死亡的显着抑制作用。 因此本发明人发现SIV-PEDF载体是用于治疗与眼组织细胞如青光眼中凋亡变性相关的疾病的有效药剂。
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2.发明申请Therapeutic Agents for Diseases Associated With Apoptotic Degeneration in Ocular Tissue Cells That Use SIV-PEDF Vectors 有权与使用SIV-PEDF载体的组织细胞凋亡相关的疾病治疗剂公开(公告)号：US20090233988A1
公开(公告)日：2009-09-17
申请号：US11884738
申请日：2006-02-21
申请人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
发明人： Masanori Miyazaki , Yoshikazu Yonemitsu , Yasuhiro Ikeda , Katsuo Sueishi , Toshiaki Tabata , Akihiro Iida , Yasuji Ueda , Mamoru Hasegawa
IPC分类号： A61K31/711 , C12N15/63 , C12N7/01
CPC分类号： A61K48/005 , A61K48/00 , C07K14/475 , C12N15/86 , C12N2740/15043 , C12N2740/15045 , C12N2810/6081
摘要： The present invention provides novel methods for treating diseases associated with apoptotic degeneration in ocular tissue cells by effective administration of pigment epithelium derived factor (PEDF). The present inventors studied PEDF as a means to prevent ganglion cell death, the final pathology of glaucoma. The present invention is particularly focused on SIV vectors for effective methods for delivering PEDF, and constructed an SIV-PEDF vector. When the SIV-PEDF vector was administered subretinally to an ischemia reperfusion model and NMDA-induced model, a significant suppression effect on ganglion cell death was observed. The present inventors therefore discovered that the SIV-PEDF vector is an effective pharmaceutical agent for treating diseases associated with apoptotic degeneration in ocular tissue cells, such as glaucoma.
摘要翻译： 本发明提供了通过有效施用色素上皮衍生因子（PEDF）来治疗与眼组织细胞凋亡变性相关的疾病的新方法。 本发明人研究了PEDF作为预防神经节细胞死亡，青光眼的最终病理学的手段。 本发明特别关注用于递送PEDF的有效方法的SIV载体，并构建了SIV-PEDF载体。 当将SIV-PEDF载体视觉上给予缺血再灌注模型和NMDA诱导的模型时，观察到对神经节细胞死亡的显着抑制作用。 因此本发明人发现SIV-PEDF载体是用于治疗与眼组织细胞如青光眼中凋亡变性相关的疾病的有效药剂。
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3.发明授权Pseudotype retroviral vectors containing membrane proteins having hemagglutinin activity 有权含有具有血凝素活性的膜蛋白的假型逆转录病毒载体公开(公告)号：US07510706B2
公开(公告)日：2009-03-31
申请号：US10306949
申请日：2002-11-29
申请人： Yoshikazu Yonemitsu , Toshihiro Nakajima , Kenji Nakamaru , Masanori Kobayashi , Mamoru Hasegawa , Yasuji Ueda , Akihiro Iida , Hiroyuki Sakakibara
发明人： Yoshikazu Yonemitsu , Toshihiro Nakajima , Kenji Nakamaru , Masanori Kobayashi , Mamoru Hasegawa , Yasuji Ueda , Akihiro Iida , Hiroyuki Sakakibara
CPC分类号： C12N7/00 , A61K48/00 , C12N15/86 , C12N2740/15043 , C12N2740/15045 , C12N2740/15052 , C12N2810/60
摘要： The present invention provides a retroviral vector containing a membrane protein having a hemagglutinin activity. The present inventors constructed a retroviral vector pseudotyped by the membrane protein having a hemagglutinin activity. This viral vector showed gene transfer at a high efficiency into host cells. In particular, it was established that genes can be transferred thereby at a high efficiency into cells into which genes can hardly be transferred by the conventional techniques, for example, blood cells and hematopoietic cells including hematopoietic stem cells, and mucous cells including mucosa epithelial cells. The viral vector of the present invention is highly useful as a vector for gene therapy.
摘要翻译： 本发明提供含有血凝素活性的膜蛋白的逆转录病毒载体。 本发明人构建了具有血细胞凝集素活性的膜蛋白假型化的逆转录病毒载体。 该病毒载体以高效率显示宿主细胞的基因转移。 特别地，已经确定，通过常规技术，例如血细胞和包括造血干细胞在内的造血细胞和包括粘膜上皮细胞的粘液细胞在基因难以转移的细胞中，可以将高效转移基因 。 本发明的病毒载体作为基因治疗载体是非常有用的。
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公开(公告)号：US20050221292A1
公开(公告)日：2005-10-06
申请号：US10513094
申请日：2003-04-30
申请人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
IPC分类号： A61K38/00 , A61K48/00 , A61P35/00 , C07K14/115 , C12N15/45 , C12N15/86 , C12Q1/70 , C12Q1/68
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。
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公开(公告)号：US20100297732A1
公开(公告)日：2010-11-25
申请号：US12712905
申请日：2010-02-25
申请人： Hiroaki KINOH , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki KINOH , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。
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公开(公告)号：US07709621B2
公开(公告)日：2010-05-04
申请号：US12140715
申请日：2008-06-17
申请人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。
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公开(公告)号：US07226786B2
公开(公告)日：2007-06-05
申请号：US10316535
申请日：2002-12-10
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Fumino Tokito , Takahiro Hirata , Tsuyoshi Tokusumi , Makoto Inoue , Yumiko Tokusumi
CPC分类号： C12N15/86 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2800/30 , C12N2810/6081
摘要： F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
摘要翻译： 通过使用F基因缺陷的仙台病毒基因组cDNA成功地回收了F基因缺陷型病毒粒子。 此外，通过使用F表达细胞作为辅助细胞，成功构建了F基因缺陷型感染性病毒颗粒。 此外，通过使用F基因和HN基因缺陷的病毒基因组cDNA，成功地回收了F基因和HN基因缺陷型病毒粒子。 此外，通过使用F-和HN表达细胞作为辅助细胞，F基因和HN基因缺陷型感染性病毒颗粒成功产生。 通过使用F表达细胞作为辅助细胞构建F基因和HN基因并具有F蛋白的病毒。 此外，使用表达M蛋白的辅助细胞产生M基因缺陷型感染性病毒颗粒。 从感染M基因缺陷病毒的细胞中，病毒样颗粒的释放被抑制。 此外，通过使用表达VSV-G的细胞成功地构建了VSV-G伪型病毒。 用于构建这些缺陷病毒的技术有助于可用于基因治疗的副粘病毒科的载体的发育。
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公开(公告)号：US20070009949A1
公开(公告)日：2007-01-11
申请号：US11515341
申请日：2006-09-01
申请人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue , Yumiko Tokusumi
发明人： Kaio Kitazato , Tsugumine Shu , Hidekazu Kuma , Yasuji Ueda , Makoto Asakawa , Mamoru Hasegawa , Akihiro Iida , Takahiro Hirata , Makoto Inoue , Yumiko Tokusumi
CPC分类号： C07K14/005 , A61K48/00 , A61K2039/5254 , A61K2039/5256 , C12N7/00 , C12N15/86 , C12N2710/24143 , C12N2760/18811 , C12N2760/18822 , C12N2760/18823 , C12N2760/18843 , C12N2760/18845 , C12N2760/18861 , C12N2760/20222 , C12N2800/30 , C12N2810/6081
摘要： A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express at least one envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.
摘要翻译： 已经成功地制备了已经被修饰以便不表达至少一种包膜蛋白的来源于仙台病毒的负链单链RNA的功能性RNP。 制备包含外来基因的RNP，并使用阳离子脂质体将其插入细胞中，从而成功地表达外源基因。
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公开(公告)号：US20080299642A1
公开(公告)日：2008-12-04
申请号：US12140715
申请日：2008-06-17
申请人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。
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公开(公告)号：US07402427B2
公开(公告)日：2008-07-22
申请号：US10513094
申请日：2003-04-30
申请人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
发明人： Hiroaki Kinoh , Makoto Inoue , Yasuji Ueda , Akihiro Iida , Mamoru Hasegawa , Masanori Kobayashi
IPC分类号： C12N15/86 , A01N63/00 , A61K39/155
CPC分类号： C07K14/005 , A61K38/00 , A61K48/00 , C07K2319/50 , C12N15/86 , C12N2760/18022 , C12N2760/18822 , C12N2760/18843 , C12N2800/30
摘要： The present invention provides cell fusogenic vectors having replicative ability, whose protease-dependent tropism has been modified. M gene-deficient viral vectors encoding modified F proteins, in which the cleavage site of the F protein of paramyxovirus is modified to be cleaved by different proteases, were produced. In cells transfected with these vectors, the genomic RNA present in the vectors is replicated, and cell fusogenic infection spreads to neighboring cells depending on the presence of other proteases; however, no viral particles are released. The vectors of this invention, encoding the F proteins which are cleaved by proteases whose activity is enhanced in cancer, show cancer growth suppressive effect in vivo.
摘要翻译： 本发明提供具有复制能力的细胞融合载体，其蛋白酶依赖性向性已被修饰。 产生编码修饰的F蛋白的M基因缺陷型病毒载体，其中修饰了被不同蛋白酶切割的副粘病毒的F蛋白的切割位点。 在用这些载体转染的细胞中，载体中存在的基因组RNA被复制，并且根据其它蛋白酶的存在，细胞融合感染扩散到相邻细胞; 然而，没有病毒颗粒被释放。 本发明的编码由蛋白酶切割的F蛋白的载体，其活性在癌症中增强，在体内显示出癌症生长抑制作用。
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