公开(公告)号：US20130204610A1

公开(公告)日：2013-08-08

申请号：US13695051

申请日：2012-04-26

申请人： Zheng Wu

发明人： Zheng Wu

IPC分类号： G06F17/28

CPC分类号： G06F17/289 ,  G06F17/279

摘要： The presented is a tool and method for language presentation, browsing, editing, translation and communication based on Semantic Web, to be utilized as interface for collaborating software products and services or human-machine interaction. The conceptual system is extended to further include such objects as language components, sentence patterns or syntax rules, to get solutions for semantic logic representation devices, language presentation devices, semantic-language converting devices, the registry and delegation system, in forming a language-component-based system for browsing, editing, conversion and communication. It is always allowed to bring need-based control over the conceptual system and the registry with their scope and scale being kept at appropriate level; with a widespread community participation, the establishment of semantic-language converting device ecosystem will be important guarantee of a flexible and diversified language expression system; therefore to constitute the core of those pragmatic standards or specifications for machine translation, human-machine interface and the web system.

摘要翻译： 提出了基于语义Web的语言呈现，浏览，编辑，翻译和通信的工具和方法，被用作协作软件产品和服务或人机交互的界面。 概念系统被扩展为进一步包括诸如语言组件，句子模式或语法规则的对象，以获得语义逻辑表示设备，语言呈现设备，语义语言转换设备，注册表和委托系统的解决方案，以形成语言 - 基于组件的系统，用于浏览，编辑，转换和通信。 总是允许对概念系统和注册表进行基于需求的控制，其范围和规模保持在适当的水平; 社区参与广泛，语义转换设备生态系统的建立将成为灵活多样的语言表达系统的重要保证; 因此构成机器翻译，人机界面和网络系统的务实标准或规范的核心。

公开(公告)号：US07782452B2

公开(公告)日：2010-08-24

申请号：US11848516

申请日：2007-08-31

申请人： Courosh Mehanian ,  Hans J. Hansen ,  Yingjian Wang ,  Yuval Ben-Dov ,  Zheng-Wu Li ,  Andrew V. Hill ,  Mehdi Vaez-Iravani ,  Kurt Zimmermann

发明人： Courosh Mehanian ,  Hans J. Hansen ,  Yingjian Wang ,  Yuval Ben-Dov ,  Zheng-Wu Li ,  Andrew V. Hill ,  Mehdi Vaez-Iravani ,  Kurt Zimmermann

IPC分类号： G01N21/00

CPC分类号： G02B21/18 ,  G01N21/8806 ,  G01N21/9501 ,  G01N2021/8825 ,  G02B21/367

摘要： A system is provided herein for inspecting a specimen. In one embodiment, the system may include a dual-channel microscope, two illuminators, each coupled for illuminating a different channel of the dual-channel microscope and two detectors, each coupled to a different channel of the dual-channel microscope for acquiring images of the specimen. Means are provided for separating the channels of the dual-channel microscope, so that the two detectors can acquire the images of the specimen at substantially the same time. In one embodiment, the channels of the dual-channel microscope may be spectrally separated by configuring the two illuminators, so that they produce light in two substantially non-overlapping spectral ranges. In another embodiment, the channels of the dual-channel microscope may be spatially separated by positioning the two detectors, so that the illumination light do not overlap and the fields of view of the two detectors do not overlap within a field of view of an objective lens included within the system.

摘要翻译： 本文提供了用于检查样本的系统。 在一个实施例中，系统可以包括双通道显微镜，两个照明器，每个照明器被耦合用于照亮双通道显微镜和两个检测器的不同通道，每个检测器耦合到双通道显微镜的不同通道，用于获取 标本。 提供了用于分离双通道显微镜的通道的装置，使得两个检测器可以在基本上同时获取样本的图像。 在一个实施例中，双通道显微镜的通道可以通过配置两个照明器进行光谱分离，使得它们产生两个基本上非重叠的光谱范围的光。 在另一个实施例中，双通道显微镜的通道可以通过定位两个检测器在空间上分离，使得照明光不重叠，并且两个检测器的视场在目标的视场内不重叠 镜头包含在系统内。

公开(公告)号：US07732129B1

公开(公告)日：2010-06-08

申请号：US09203078

申请日：1998-12-01

申请人： Shuyuan Zhang ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho ,  Shawn Gallagher

发明人： Shuyuan Zhang ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho ,  Shawn Gallagher

IPC分类号： C12Q1/70

CPC分类号： C07K14/4746 ,  A61K35/761 ,  A61K38/00 ,  A61K48/00 ,  C12N7/00 ,  C12N15/86 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2710/10352 ,  C12N2750/14143

摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

摘要翻译： 本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。 特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。 在其他实施方案中，本发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的细胞，存在改善的Ad-p53产生。 提高产量也很重要的是使用洗涤剂溶解。 本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

公开(公告)号：US20100055763A1

公开(公告)日：2010-03-04

申请号：US12572943

申请日：2009-10-02

申请人： SHUYUAN ZHANG ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho

发明人： SHUYUAN ZHANG ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho

IPC分类号： C12N7/02

CPC分类号： C12N7/00 ,  A61K35/761 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746 ,  C12N15/86 ,  C12N2710/10045 ,  C12N2710/10051 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2810/6018

摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production with cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

摘要翻译： 本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。 特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。 在其他实施方案中，本发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的细胞，存在改善的Ad-p53产生。 提高产量也很重要的是使用洗涤剂溶解。 本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

公开(公告)号：US07445930B2

公开(公告)日：2008-11-04

申请号：US10909066

申请日：2004-07-30

申请人： Shuyuan Zhang ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho ,  Deborah Wilson ,  Lucetta Caston

发明人： Shuyuan Zhang ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho ,  Deborah Wilson ,  Lucetta Caston

IPC分类号： C12N15/00 ,  A61K39/00 ,  A61K39/23 ,  A61K39/095

CPC分类号： C12N7/00 ,  A61K35/761 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746 ,  C12N15/86 ,  C12N2710/10045 ,  C12N2710/10051 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2810/6018

摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production witrh cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

摘要翻译： 本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。 特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。 在其它实施方案中，本发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的细胞，具有改善的Ad-p53产生。 提高产量也很重要的是使用洗涤剂溶解。 本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

公开(公告)号：US20080107631A1

公开(公告)日：2008-05-08

申请号：US11768796

申请日：2007-06-26

申请人： Zheng Wu ,  Shuyuan Zhang

发明人： Zheng Wu ,  Shuyuan Zhang

IPC分类号： A01N63/00

CPC分类号： C12N7/00 ,  A61K9/0019 ,  A61K9/19 ,  A61K35/761 ,  A61K47/02 ,  A61K47/183 ,  A61K47/20 ,  A61K47/26 ,  A61K47/32 ,  A61K47/38 ,  A61K47/42 ,  A61K47/46 ,  A61K48/00 ,  C12N15/86 ,  C12N2710/10051 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10351

摘要： The present invention addresses the need to improve the long-term storage stability (i.e. infectivity) of vector formulations. In particular, it has been demonstrated that for adenovirus, the use of bulking agents, cryoprotectants and lyoprotectants imparts desired properties that allow both lyophilized and liquid adenovirus formulations to be stored at 4° C. for up to 6 months and retain an infectivity between 60-100% of the starting infectivity.

摘要翻译： 本发明解决了改善载体制剂的长期储存稳定性（即感染性）的需要。 特别地，已经证明，对于腺病毒，使用填充剂，冷冻保护剂和冻干保护剂赋予所需的性质，其允许冻干和液体腺病毒制剂在4℃下储存长达6个月并保持在60 -100％的起始传染性。

公开(公告)号：US20060253967A1

公开(公告)日：2006-11-16

申请号：US11429244

申请日：2006-05-08

申请人： Zheng Wu

发明人： Zheng Wu

IPC分类号： E03D9/08 ,  A47K3/20 ,  A47K4/00

CPC分类号： A61H35/00 ,  A61H2201/1633 ,  A61H2205/085 ,  A61H2205/086 ,  A61H2205/087

摘要： A hydrotherapy device comprising a primary unit, a conduit, and a control panel electrically connected to the primary conduit for controlling the treatment of water. The primary unit includes a filter unit, a heater for heating the water, and a pressure regulator for pressurizing the water. The conduit directs water that is pre-treated by the primary unit out for use in hydrotherapy. The invention connects to an existing water supply system and pre-treats the water for use in hydrotherapy to be of higher quality and more purified so as to ensure the usage safety and reduce the chance of the user feeling discomforts or getting infections.

摘要翻译： 一种水疗装置，包括主要单元，导管和电连接到主导管的控制面板，用于控制水的处理。 主要单元包括过滤器单元，用于加热水的加热器和用于对水进行加压的压力调节器。 导管将主要单元预先处理的水引导到水疗中。 本发明连接到现有的供水系统，并且将用于水疗的水预处理更高质量和更纯化，以确保使用安全性并减少使用者感到不舒服或感染的机会。

公开(公告)号：US06422701B1

公开(公告)日：2002-07-23

申请号：US09928164

申请日：2001-08-10

申请人： Jay Wei ,  Zheng-wu Li ,  Shiyu Zhang

发明人： Jay Wei ,  Zheng-wu Li ,  Shiyu Zhang

IPC分类号： A61B302

摘要： One embodiment of the present invention is a visual field tester that includes: (a) a high intensity light source; (b) an optical fiber; (c) scanning optics, wherein light output from the light source is directed by the optical fiber to impinge upon the scanning optics, and the scanning optics directs the light to form a stimulus at various field positions; and (d) a video display system to output one or more light patterns. Another embodiment of the present invention is a visual field tester that includes: (a) a video display system to output one or more types of light patterns; (b) a background illumination display system; and (c) viewing optics to magnify the field of view of the video display system and the background illumination display system. Still another embodiment of the present invention is a visual field tester that includes: (a) a video display system to output one or more types of light patterns; and (b) a background illumination display system that outputs variable intensity light, wherein the video display system includes: (i) a variable intensity, high brightness light source and (ii) a light modulator display disposed to transmit light output from the source.

公开(公告)号：US06194191B1

公开(公告)日：2001-02-27

申请号：US08975519

申请日：1997-11-20

申请人： Shuyuan Zhang ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho

发明人： Shuyuan Zhang ,  Capucine Thwin ,  Zheng Wu ,  Toohyon Cho

IPC分类号： C12N702

CPC分类号： C12N7/00 ,  A61K35/761 ,  A61K38/00 ,  A61K48/00 ,  C07K14/4746 ,  C12N15/86 ,  C12N2710/10045 ,  C12N2710/10051 ,  C12N2710/10322 ,  C12N2710/10332 ,  C12N2710/10343 ,  C12N2710/10351 ,  C12N2810/6018

摘要： The present invention addresses the need to improve the yields of viral vectors when grown in cell culture systems. In particular, it has been demonstrated that for adenovirus, the use of low-medium perfusion rates in an attached cell culture system provides for improved yields. In other embodiments, the inventors have shown that there is improved Ad-p53 production witrh cells grown in serum-free conditions, and in particular in serum-free suspension culture. Also important to the increase of yields is the use of detergent lysis. Combination of these aspects of the invention permits purification of virus by a single chromatography step that results in purified virus of the same quality as preparations from double CsCl banding using an ultracentrifuge.

摘要翻译： 本发明解决了在细胞培养系统中生长时提高病毒载体产量的需要。 特别地，已经证明，对于腺病毒，在附着的细胞培养系统中使用低中等灌注速率提供了提高的产量。 在其它实施方案中，本发明人已经表明，在无血清条件下，特别是在无血清悬浮培养物中生长的细胞，具有改善的Ad-p53产生。 提高产量也很重要的是使用洗涤剂溶解。 本发明的这些方面的组合允许通过单个色谱步骤纯化病毒，其导致与使用超速离心机的双CsCl条带制备相同质量的纯化病毒。

公开(公告)号：US08093933B2

公开(公告)日：2012-01-10

申请号：US12606195

申请日：2009-10-27

申请人： Yong Wang ,  Odi Dahan ,  Zheng Wu ,  Jianbin Zhao

发明人： Yong Wang ,  Odi Dahan ,  Zheng Wu ,  Jianbin Zhao

IPC分类号： H03L7/00 ,  G06F17/17

CPC分类号： H03H17/0219 ,  H03H17/0628

摘要： A method for fast tracking and jitter improvement in an asynchronous sample rate conversion includes a digital phase locked loop (DPLL) for an asynchronous sample rate conversion (ASRC) device. A control apparatus in the DPLL includes a gain controller that sets and maintains gains (Ki, Kp) of two branches of the control apparatus at a fixed value, which enables searching of a desired value by the DPLL to determine a neighborhood of the desired value, and reduces the gains when the number of samples reaches a predetermined number. Processing units in the DPLL generate and process first and second input signals based on an input clock, an output clock, and a system clock. The second input signal is processed using two branches. Signals resulting from the two branches are re-aligned according to a changed status of the first processed input signal such that the signals resulting from the two branches are sampled in the same input clock interval.

摘要翻译： 用于异步采样率转换中的快速跟踪和抖动改进的方法包括用于异步采样率转换（ASRC）设备的数字锁相环（DPLL）。 DPLL中的控制装置包括增益控制器，其以固定值设置和维持控制装置的两个分支的增益（Ki，Kp），从而能够通过DPLL搜索期望值以确定期望值的邻域 并且当样本数量达到预定数量时降低增益。 DPLL中的处理单元基于输入时钟，输出时钟和系统时钟生成并处理第一和第二输入信号。 使用两个分支处理第二个输入信号。 根据第一处理输入信号的改变状态，由两个分支产生的信号被重新对齐，使得由两个分支产生的信号在相同的输入时钟间隔中进行采样。