公开(公告)号：US20240035072A1

公开(公告)日：2024-02-01

申请号：US18360137

申请日：2023-07-27

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON

IPC: C12Q1/6841 ,  G01N21/64

CPC classification number: C12Q1/6841 ,  G01N21/6428 ,  G01N2021/6439

Abstract: The present disclosure generally relates to methods and compositions for in situ analysis or detection of analytes in a sample. More specifically, the present disclosure relates to methods for reducing autofluorescence in tissue samples, methods for analyzing biological samples, and compounds for use in the same. The methods and compounds of the present disclosure may be especially suitable for analytical methods employing fluorescence in situ hybridization techniques over multiple cycles of imaging.

公开(公告)号：US20240167081A1

公开(公告)日：2024-05-23

申请号：US18503949

申请日：2023-11-07

Applicant: 10x Genomics,Inc.

Inventor： Felice Alessio BAVA ,  Cheyenne CHRISTOPHERSON ,  Justin COSTA ,  Shalini GOHIL ,  Christina GALONSKA ,  Monica NAGENDRAN

IPC: C12Q1/682 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30

CPC classification number: C12Q1/682 ,  C12Q1/6841 ,  C12Q1/6844 ,  C12Q1/6876 ,  G01N1/30

Abstract: The present disclosure relates in some aspects to methods and compositions for immobilization of nucleic acids for in situ detection in a biological sample or a matrix embedding the biological sample.

公开(公告)号：US20240376521A1

公开(公告)日：2024-11-14

申请号：US18661391

申请日：2024-05-10

Applicant: 10x Genomics, Inc.

Inventor： Justin COSTA ,  Cheyenne CHRISTOPHERSON

IPC: C12Q1/6806 ,  C12N9/12 ,  C12N9/22 ,  C12Q1/6876

Abstract: The present disclosure relates in some aspects to methods and boronic acid compositions for immobilizing RNA analytes in biological samples, and more specifically fragmented RNAs. RNA analytes may be tethered covalently or non-covalently to exogenous or endogenous molecules in a biological sample, for example, cross-linked directly to a polymerized three-dimensional matrix.

公开(公告)号：US20240351041A1

公开(公告)日：2024-10-24

申请号：US18634751

申请日：2024-04-12

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON ,  Justin COSTA ,  Shalini GOHIL ,  Hong CHEN ,  Yufeng QIAN ,  Minkyung PARK ,  Xiaoyang CAI ,  Hiroshi SASAKI ,  Naveed BAKH

IPC: B01L3/00

CPC classification number: B01L3/527 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2200/16 ,  B01L2300/044 ,  B01L2300/12

Abstract: The present disclosure provides systems and methods for reagent storage. System may include one or more substrates comprising a partition. The partition may be configured to retain a first reagent separate from a second reagent. The partition may include a seal disposed between the first and second reagent. The seal may be configured to maintain the first reagent separate from the second reagent. The first and second reagent may be disposed in a first layer and a second layer, respectively, of frozen material. The first layer and second layer of frozen material may maintain the first reagent separate from the second reagent.

公开(公告)号：US20240191297A1

公开(公告)日：2024-06-13

申请号：US18486918

申请日：2023-10-13

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON ,  Veronica Emelina GONZALEZ MUÑOZ ,  Joshua GU ,  Albert Dale KIM ,  Yi LUO ,  Meiliana TJANDRA

IPC: C12Q1/6881 ,  C12Q1/6806 ,  C12Q1/6841

CPC classification number: C12Q1/6881 ,  C12Q1/6806 ,  C12Q1/6841 ,  C12Q2600/166

Abstract: The present disclosure relates in some aspects to methods and compositions for quality control (e.g., assessing the level of fixation of a biological sample) and/or optimization of analyte detection in fixed samples. In some embodiments, the method can be used to process a fixed biological sample, the method comprising contacting the fixed biological sample with a nucleic acid stain and/or an actin stain; detecting an optical signal associated with the nucleic acid stain and/or an optical signal associated with the actin stain; comparing the detected optical signal(s) to one or more references to determine the quality of the biological sample. Single cell analysis, spatial array based analysis or in situ analysis can be performed using the processed biological sample.

公开(公告)号：US20240035070A1

公开(公告)日：2024-02-01

申请号：US18360135

申请日：2023-07-27

Applicant: 10x Genomics, Inc.

Inventor： Cheyenne CHRISTOPHERSON

IPC: C12Q1/6837 ,  C12Q1/6841

CPC classification number: C12Q1/6837 ,  C12Q1/6841

Abstract: The present disclosure generally relates to methods and compositions for detecting a plurality of molecules of one or more analytes in a sample. In some aspects, the present disclosure relates to methods for determining the locations and identities of analytes in a biological sample using detectably labeled probes comprising detectable labels with different signal emission lifetimes. In some aspects, the present disclosure relates to methods for identifying the detectable labels of the detectably labeled probes using time-gated detection. The methods herein have particular applicability in the detection of identifier sequences (e.g., analyte sequences or barcode sequences) in situ in a biological sample, including those using sequential cycles of detectably labeled probe hybridization to decode the identifier sequences.