公开(公告)号：US10287553B2

公开(公告)日：2019-05-14

申请号：US14395811

申请日：2013-04-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Daniele Zink ,  Ming Ni ,  Karthikeyan Narayanan ,  Karthikeyan Kandasamy ,  Andrew C. A. Wan ,  Jackie Y. Ying

IPC: C12N5/074 ,  C12N5/00 ,  C12N5/0735 ,  C12N5/0775

Abstract: There is provided a method for culturing a stem cell in vitro. The method comprises providing a substrate surface coated with a coating comprising a molecule having a catechol moiety or a polymer thereof; and growing a stem cell on said coated substrate surface in a growth medium.

公开(公告)号：US20170196993A1

公开(公告)日：2017-07-13

申请号：US15324144

申请日：2015-07-08

Applicant: Agency for Science, Technology and Research

Inventor： Charlotte Hauser ,  Ming Ni

IPC: A61K49/00 ,  A61L27/22 ,  C12Q1/02 ,  A61K9/14 ,  A61K47/42 ,  A61L26/00 ,  A61L27/52

CPC classification number: A61K49/00 ,  A61K9/14 ,  A61K47/42 ,  A61L26/0047 ,  A61L26/008 ,  A61L27/227 ,  A61L27/52 ,  C07K5/06034 ,  C07K5/0808 ,  C07K5/101 ,  C07K14/78 ,  C12Q1/02

Abstract: Various aspects of the present invention relate to a peptide based biomaterial for visualization by SHG microscopy. In particular the invention relates to the use of short peptides as a non-linear optical (NLO) material for second harmonic generation (SHG) microscopy. A preferred short peptide comprises LIVAGK (LK6) and contains a non-polar aliphatic tail (with decreasing hydrophobicity) and a polar head; and can self-assemble into hydrogels; wherein which the peptide forms a tunable fibrous structure for in vitro and in vivo imaging applications and is suitable in disease diagnostics such as amyloidosis, including 1) neuro-degenerative amyloidosis, e.g. Alzheimer's (AD), Parkinson's, Huntington's (PD), 2) non-neuropathic localized amyloidosis such as in Type II Diabetes, and 3) systemic amyloidosis that occurs in multiple tissues, e.g. cataracts and lattice corneal dystrophy (LCD), as well as drug delivery and/or wound dressings.

公开(公告)号：US20150087057A1

公开(公告)日：2015-03-26

申请号：US14395811

申请日：2013-04-22

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Daniele Zink ,  Ming Ni ,  Karthikeyan Narayanan ,  Karthikeyan Kandasamy ,  Andrew C.A. Wan ,  Jackie Y. Ying

IPC: C12N5/074 ,  C12N5/0775 ,  C12N5/0735

CPC classification number: C12N5/0696 ,  C12N5/0068 ,  C12N5/0075 ,  C12N5/0606 ,  C12N5/0663 ,  C12N2500/90 ,  C12N2501/815 ,  C12N2531/00 ,  C12N2533/00 ,  C12N2533/20 ,  C12N2533/30 ,  C12N2533/50 ,  C12N2533/70 ,  C12N2533/78

Abstract: There is provided a method for culturing a stem cell in vitro. The method comprises providing a substrate surface coated with a coating comprising a molecule having a catechol moiety or a polymer thereof; and growing a stem cell on said coated substrate surface in a growth medium.

Abstract translation: 提供了体外培养干细胞的方法。 该方法包括提供涂覆有包含具有儿茶酚部分或其聚合物的分子的涂层的基底表面; 以及在生长培养基中在所述涂覆的基底表面上生长干细胞。

公开(公告)号：US10105454B2

公开(公告)日：2018-10-23

申请号：US15324144

申请日：2015-07-08

Applicant: Agency for Science, Technology and Research

Inventor： Charlotte Hauser ,  Ming Ni

IPC: A61K49/00 ,  A61L26/00 ,  C12Q1/02 ,  A61L27/52 ,  A61K9/14 ,  A61K47/42 ,  A61L27/22

Abstract: Various aspects of the present invention relate to a peptide based biomaterial for visualization by SHG microscopy. In particular the invention relates to the use of short peptides as a non-linear optical (NLO) material for second harmonic generation (SHG) microscopy. A preferred short peptide comprises LIVAGK (LK6) and contains a non-polar aliphatic tail (with decreasing hydrophobicity) and a polar head; and can self-assemble into hydrogels; wherein which the peptide forms a tunable fibrous structure for in vitro and in vivo imaging applications and is suitable in disease diagnostics such as amyloidosis, including 1) neuro-degenerative amyloidosis, e.g. Alzheimer's (AD), Parkinson's, Huntington's (PD), 2) non-neuropathic localized amyloidosis such as in Type II Diabetes, and 3) systemic amyloidosis that occurs in multiple tissues, e.g. cataracts and lattice corneal dystrophy (LCD), as well as drug delivery and/or wound dressings.

公开(公告)号：US20180036432A9

公开(公告)日：2018-02-08

申请号：US15324144

申请日：2015-07-08

Applicant: Agency for Science, Technology and Research

Inventor： Charlotte Hauser ,  Ming Ni

IPC: A61K49/00 ,  A61L27/22 ,  C12Q1/02 ,  A61K9/14 ,  A61K47/42 ,  A61L26/00 ,  A61L27/52

CPC classification number: A61K49/00 ,  A61K9/14 ,  A61K47/42 ,  A61L26/0047 ,  A61L26/008 ,  A61L27/227 ,  A61L27/52 ,  C07K5/06034 ,  C07K5/0808 ,  C07K5/101 ,  C07K14/78 ,  C12Q1/02

Abstract: Various aspects of the present invention relate to a peptide based biomaterial for visualization by SHG microscopy. In particular the invention relates to the use of short peptides as a non-linear optical (NLO) material for second harmonic generation (SHG) microscopy. A preferred short peptide comprises LIVAGK (LK6) and contains a non-polar aliphatic tail (with decreasing hydrophobicity) and a polar head; and can self-assemble into hydrogels; wherein which the peptide forms a tunable fibrous structure for in vitro and in vivo imaging applications and is suitable in disease diagnostics such as amyloidosis, including 1) neuro-degenerative amyloidosis, e.g. Alzheimer's (AD), Parkinson's, Huntington's (PD), 2) non-neuropathic localized amyloidosis such as in Type II Diabetes, and 3) systemic amyloidosis that occurs in multiple tissues, e.g. cataracts and lattice corneal dystrophy (LCD), as well as drug delivery and/or wound dressings.