公开(公告)号：US12196741B2

公开(公告)日：2025-01-14

申请号：US17425571

申请日：2020-01-27

Applicant: AXION BIOSYSTEMS, INC.

Inventor： Michael Antony Clements ,  Isaac Perry Clements ,  Thomas J. O'Brien ,  James David Ross ,  Daniel Christopher Millard ,  Andrew Willsie ,  Edgar A. Brown ,  Robert Dixon Grier, Jr. ,  Amanda Jervis Preyer ,  Denise Danielle Sullivan

IPC: G01N33/483 ,  G01N21/59 ,  G01N27/02 ,  G01N33/487

Abstract: A scalable, real-time, label-free, electrode- or optical-based cell monitoring system for integration into a cell culture incubator is described herein. An example system includes (1) cell culture consumables with integrated electrodes and/or optics for growing and monitoring cells, (2) incubator trays for consumable organization and recording, and (3) a system console, external to the incubator, for connecting multiple incubator trays. Without perturbing the cell culture, the system is capable of monitoring multiple culture attributes for each cell culture consumable simultaneously. These attributes can include, but are not limited to, cell growth, proliferation, morphology, media pH, or media oxygen. The system can support multiple trays, which permits monitoring dozens to hundreds of consumables simultaneously, including a mixture of consumables of various sizes. In addition to monitoring adherent cells, the disclosed technology can be readily adapted for monitoring of cell suspensions.

公开(公告)号：US10351812B2

公开(公告)日：2019-07-16

申请号：US14838575

申请日：2015-08-28

Applicant: Axion BioSystems, Inc.

Inventor： Robert Dixon Grier, Jr. ,  David Ernest Irvine ,  James David Ross

IPC: C12M1/00 ,  C12M1/12 ,  C12M1/32 ,  C12M1/34 ,  C12M3/00

Abstract: Systems and methods for creating a localized environment for a cell culture plate are described herein. An example system can include a dock shaped to receive the cell culture plate, and a gas distribution device positioned on the dock. The gas distribution device can include a frame including a plurality of sides connected to form an opening, an internal channel within the frame for directing a gas mixture within the gas distribution device, an inlet port in fluid communication with the internal channel for receiving the gas mixture, and a plurality of apertures in fluid communication with the internal channel. The plurality of apertures can be spaced apart from one another along the frame and configured to direct the gas mixture toward the opening.

公开(公告)号：US09885012B2

公开(公告)日：2018-02-06

申请号：US14533373

申请日：2014-11-05

Applicant: AXION BIOSYSTEMS, INC.

Inventor： Robert Dixon Grier, Jr. ,  Anthony Michael Nicolini ,  Colin Michael Arrowood ,  Swaminathan Rajaraman

IPC: B01L1/00 ,  B01L3/00 ,  C12M1/00 ,  G01N33/487 ,  C12M1/32 ,  C12M1/26 ,  C12M1/42 ,  C12M1/34 ,  C12M1/36

CPC classification number: C12M23/38 ,  C12M23/00 ,  C12M23/12 ,  C12M33/04 ,  C12M35/02 ,  C12M41/12 ,  C12M41/26 ,  C12M41/32 ,  C12M41/36 ,  C12M41/46 ,  C12M41/48 ,  G01N33/48728

Abstract: Devices, systems, and methods for facilitating placement of cells and materials in culture plates configured for high-throughput applications are provided. A culture system is provided with a culture plate having a lid for guiding placement of cells and materials in each individual culture well of a culture plate. The lid may provide for coupling to an electrophysiology culture plate comprising a biosensor plate and a biologic culture plate, where the biosensor plate underlies and is coupled to the culture well plate such that each biosensor is operatively coupled to one culture well of the plurality of culture wells. A containment device that physically influences the positioning of fluid received in the culture plate is also provided herein.