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    Reagents and methods for solid phase synthesis and display
    3.
    发明申请
    Reagents and methods for solid phase synthesis and display 审中-公开
    用于固相合成和显示的试剂和方法

    公开(公告)号：US20040106728A1

    公开(公告)日：2004-06-03

    申请号：US10619799

    申请日：2003-07-14

    Applicant: Affymetrix, Inc.

    Inventor： Glenn H. McGall ,  Anthony D. Barone ,  Martin Diggelmann

    IPC: C08G063/48 ,  C08G063/91 ,  C07F009/02

    CPC classification number: C07F9/2408 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07D493/10 ,  C07H21/00 ,  C40B40/06 ,  C40B40/10

    Abstract: New compounds, compositions and methods which find application in solid phase synthesis including the preparation of high-density arrays of diverse polymer sequences such as diverse peptides and oligonucleotides as well as in preparation of arrays of small ligand molecules. The compounds of the present invention are those which are typically referred to as linking groups, linkers or spacers and include unsymmetrical disulfide linking groups, and 1,3-diol derivatives capable of providing a triggered release of an attached compound from a solid support under mild conditions. Additional new compounds are labels which can be incorporated into either the 3null or 5null terminus of a DNA oligomer.

    Abstract translation: 可用于固相合成的新化合物，组合物和方法，包括制备不同聚合物序列的高密度阵列，例如多种肽和寡核苷酸，以及制备小配体分子的阵列。 本发明的化合物是通常称为连接基团，接头或间隔基并且包括不对称二硫键连接基团的化合物，以及能够在温和的条件下从固体支持物引发附着化合物的触发释放的1,3-二醇衍生物 条件。 另外的新化合物是可掺入DNA寡聚体的3'或5'末端的标记。

    Nucleic acid labeling compounds
    5.
    发明申请
    Nucleic acid labeling compounds 有权
    核酸标记化合物

    公开(公告)号：US20040002595A1

    公开(公告)日：2004-01-01

    申请号：US10452519

    申请日：2003-06-02

    Applicant: Affymetrix, Inc.

    Inventor： Glenn McGall ,  Anthony D. Barone

    IPC: C07H021/04 ,  C07H019/052 ,  C07D403/02 ,  C07D45/02

    CPC classification number: C07H19/052 ,  C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/12 ,  C07H21/00

    Abstract: Nucleic acid labeling compounds containing heterocyclic derivatives are disclosed. The heterocyclic derivative containing compounds are synthesized by condensing a heterocyclic derivative with a cyclic group (e.g. a ribofuranose derivative). The labeling compounds are suitable for enzymatic attachment to a nucleic acid, either terminally or internally, to provide a mechanism of nucleic acid detection.

    Abstract translation: 公开了含有杂环衍生物的核酸标记化合物。 含杂环衍生物的化合物是通过将杂环衍生物与环状基团（例如呋喃核糖衍生物）缩合而合成的。 标记化合物适合于在末端或内部酶促附着于核酸，以提供核酸检测机制。

    Nucleic acid labeling methods
    7.
    发明申请
    Nucleic acid labeling methods 审中-公开
    核酸标记方法

    公开(公告)号：US20040086914A1

    公开(公告)日：2004-05-06

    申请号：US10617992

    申请日：2003-07-11

    Applicant: Affymetrix, INC.

    Inventor： Kyle B. Cole ,  Vivi Truong ,  Glenn H. McGall ,  Anthony D. Barone

    IPC: C12Q001/68 ,  C07H021/02 ,  C07H021/04 ,  C12P019/34

    CPC classification number: C07H21/00 ,  C07H19/10 ,  C07H19/20 ,  C12Q1/6816 ,  C12Q2565/501 ,  C12Q2563/107 ,  C12Q2521/501

    Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one embodiment, T4 RNA ligase is used to attach a 3null-biotinylated AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3null-AppN-3null-linker-biotin is used as donor molecule. In another aspect of the invention, a method is provided for analyzing a nucleic acid population on a nucleic acid microarray comprising providing a nucleic acid population or converting the nucleic acid population into nucleic acid fragments; ligating the nucleic acid population or fragments to a labeled nucleic acid molecule to form labeled nucleic acid population or fragments using a ligase; hybridizing the labeled nucleic acid population or fragments to an array of nucleic acid probes, and determining hybridization signals of the probes as an indication of levels of the nucleic acids in the nucleic acid population.

    Abstract translation: 在本发明的一个方面，提供了用于末端标记RNA（总RNA，mRNA，cRNA或片段化RNA）的方法。 在一个实施方案中，T4 RNA连接酶用于将3'-生物素化的AMP或CMP供体连接到RNA受体分子。 在另一个实施方案中，焦磷酸盐分子3'-AppN-3'-接头 - 生物素用作供体分子。 在本发明的另一方面，提供了一种用于分析核酸微阵列上的核酸群的方法，包括提供核酸群体或将核酸群体转化为核酸片段; 将核酸群体或片段连接到标记的核酸分子以使用连接酶形成标记的核酸群体或片段; 将标记的核酸群体或片段与核酸探针阵列杂交，以及确定探针的杂交信号作为核酸群体中核酸水平的指示。

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