公开(公告)号：US20040076987A1

公开(公告)日：2004-04-22

申请号：US10457994

申请日：2003-06-10

Applicant: Affymetrix, Inc.

Inventor： Glenn McGall ,  Anthony D. Barone ,  Martin Diggelmann ,  David J. Lockhart ,  Ann Maria Caviani Pease ,  Mark Chee

IPC: B05D003/00 ,  C12Q001/68 ,  C12M001/34

CPC classification number: B82Y30/00 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00693 ,  B01J2219/00711 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/807

Abstract: Methods for testing oligonucleotide arrays are disclosed including methods for testing the efficiency of nucleotide coupling; methods for testing amounts of deprotected oligonucleotides; methods for determining amounts of depurinated oligonucleotides; and methods of detecting the presence of cleavable structural features, such as double-stranded nucleic acids

Abstract translation: 公开了测试寡核苷酸阵列的方法，包括测试核苷酸偶联效率的方法; 检测去保护的寡核苷酸的量的方法; 用于测定去酰基化寡核苷酸量的方法; 以及检测可切割结构特征如双链核酸的存在的方法

公开(公告)号：US20020009729A1

公开(公告)日：2002-01-24

申请号：US09781537

申请日：2001-02-08

Applicant: Affymetrix, Inc.

Inventor： Glenn McGall ,  Anthony D. Barone ,  Martin Diggelmann ,  David J. Lockhart ,  Ann Maria Caviani Pease ,  Mark Chee

IPC: C12Q001/68 ,  C12P019/34

CPC classification number: B82Y30/00 ,  B01J2219/00432 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00693 ,  B01J2219/00711 ,  B01J2219/00722 ,  C12Q1/6837 ,  C40B40/06 ,  C40B60/14 ,  Y10S435/807

Abstract: Methods for testing oligonucleotide arrays are disclosed including methods for testing the efficiency of nucleotide coupling; methods for testing amounts of deprotected oligonucleotides; methods for determining amounts of depurinated oligonucleotides; and methods of detecting the presence of cleavable structural features, such as double-stranded nucleic acids.

Abstract translation: 公开了测试寡核苷酸阵列的方法，包括测试核苷酸偶联效率的方法; 检测去保护的寡核苷酸的量的方法; 用于测定去酰基化寡核苷酸量的方法; 以及检测可切割结构特征如双链核酸的存在的方法。

公开(公告)号：US20040106728A1

公开(公告)日：2004-06-03

申请号：US10619799

申请日：2003-07-14

Applicant: Affymetrix, Inc.

Inventor： Glenn H. McGall ,  Anthony D. Barone ,  Martin Diggelmann

IPC: C08G063/48 ,  C08G063/91 ,  C07F009/02

CPC classification number: C07F9/2408 ,  B01J2219/00722 ,  B01J2219/00725 ,  C07D493/10 ,  C07H21/00 ,  C40B40/06 ,  C40B40/10

Abstract: New compounds, compositions and methods which find application in solid phase synthesis including the preparation of high-density arrays of diverse polymer sequences such as diverse peptides and oligonucleotides as well as in preparation of arrays of small ligand molecules. The compounds of the present invention are those which are typically referred to as linking groups, linkers or spacers and include unsymmetrical disulfide linking groups, and 1,3-diol derivatives capable of providing a triggered release of an attached compound from a solid support under mild conditions. Additional new compounds are labels which can be incorporated into either the 3null or 5null terminus of a DNA oligomer.

Abstract translation: 可用于固相合成的新化合物，组合物和方法，包括制备不同聚合物序列的高密度阵列，例如多种肽和寡核苷酸，以及制备小配体分子的阵列。 本发明的化合物是通常称为连接基团，接头或间隔基并且包括不对称二硫键连接基团的化合物，以及能够在温和的条件下从固体支持物引发附着化合物的触发释放的1,3-二醇衍生物 条件。 另外的新化合物是可掺入DNA寡聚体的3'或5'末端的标记。

公开(公告)号：US20030049674A1

公开(公告)日：2003-03-13

申请号：US10278970

申请日：2002-10-22

Applicant: Affymetrix, Inc.

Inventor： David J. Lockhart ,  Dirk Vetter ,  Martin Diggelmann

IPC: C12Q001/68 ,  C07H021/04 ,  C12M001/34

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00608 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00722 ,  C07B2200/11 ,  C07H21/00 ,  C07K7/06 ,  C12Q1/6811 ,  C12Q1/6837 ,  C40B40/00 ,  C40B40/06 ,  G01N33/5308 ,  G01N33/551 ,  C12Q2565/525 ,  C12Q2525/205

Abstract: Libraries of unimolecular, double-stranded oligonucleotides on a solid support. These libraries are useful in pharmaceutical discovery for the screening of numerous biological samples for specific interactions between the double-stranded oligonucleotides, and peptides, proteins, drugs and RNA. In a related aspect, the present invention provides libraries of conformationally restricted probes on a solid support. The probes are restricted in their movement and flexibility using double-stranded oligonucleotides as scaffolding. The probes are also useful in various screening procedures associated with drug discovery and diagnosis. The present invention further provides methods for the preparation and screening of the above libraries.