公开(公告)号：US20230339983A1

公开(公告)日：2023-10-26

申请号：US18182332

申请日：2023-03-11

Applicant: Agency for Science, Technology and Research

Inventor： Sandhya Sriram ,  Shigeki Sugii ,  Young Tae Chang ,  Nam-Young Kang

IPC: C07F5/02 ,  C12N5/0735 ,  C12N5/074 ,  G01N33/58

CPC classification number: C07F5/022 ,  C12N5/0606 ,  C12N5/0696 ,  G01N33/582

Abstract: Determining, in a sample, the presence and/or amount of pluripotent stem cells. A sample suspected of containing one or more pluripotent stem cells is provided. The sample with a fluorescent compound is contacted under conditions that allow binding of the fluorescent compound to the pluripotent stem cells, where the fluorescent compound is a fluorescent compound of formula (I) or a pharmaceutically acceptable salt thereof. The presence and/or amount of the pluripotent stem cells is determined by measuring the fluorescence of the cells following the contacting.

公开(公告)号：US11634437B2

公开(公告)日：2023-04-25

申请号：US16620792

申请日：2018-07-04

Applicant: Agency for Science, Technology and Research

Inventor： Sandhya Sriram ,  Shigeki Sugii ,  Young Tae Chang ,  Nam-Young Kang

IPC: G01N33/58 ,  C12N5/0735 ,  C07F5/02 ,  C12N5/074

Abstract: The present invention provides fluorescent compounds of formula (I) as disclosed herein or pharmaceutically acceptable salts thereof. Further provided are uses of said fluorescent compounds in methods of determining, in a sample, the presence and/or amount of pluripotent stem cells or cells undergoing reprogramming to become induced pluripotent stem cells.

公开(公告)号：US20190086389A1

公开(公告)日：2019-03-21

申请号：US16100152

申请日：2018-08-09

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Shigeki Sugii ,  Wee Kiat Ong ,  Weiping Han

IPC: G01N33/50 ,  G01N33/68 ,  C12N5/0775

Abstract: Disclosed is a method of identifying the origin of adipose-derived stem cells, comprising detecting at least one cell-surface marker selected from a group consisting of CD 10, CD141, CD 142 and CD200. Also disclosed are methods of determining adipogenic capability of a cell capable of adipogenesis by determining the presence of CD 10 or CD200.

公开(公告)号：US20240309326A1

公开(公告)日：2024-09-19

申请号：US18577873

申请日：2022-07-08

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Jian Ming Lamony Chew ,  Khin Oo Angela Lwin ,  Yeh Qi Cheryl Wong ,  Shigeki Sugii

IPC: C12N5/077

CPC classification number: C12N5/0653 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2531/00

Abstract: This technology relates to a method of isolating adipose-derived cell lines from an animal, a method of isolating adipocytes from an animal, and isolated adipose-derived cell lines thereof.

公开(公告)号：US20160143955A1

公开(公告)日：2016-05-26

申请号：US14903672

申请日：2014-07-10

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Shigeki Sugii ,  Wee Kiat Ong

IPC: A61K35/35 ,  C12N5/077

CPC classification number: A61K35/35 ,  A61K35/12 ,  A61K35/36 ,  C12N5/0653 ,  C12N5/0667 ,  C12N2527/00

Abstract: Disclosed is a method for recovering stromal vascular fraction from a sample. The method comprises providing a mechanical force capable of breaking the sample into a single cell suspension whilst maintaining intact stromal vascular fraction cells cellular structures, wherein the mechanical force is mincing and/or homogenization. Also disclosed are methods of using the stromal vascular fraction.

Abstract translation: 公开了一种从样品中回收基质血管部分的方法。 该方法包括提供能够将样品破碎成单细胞悬浮液同时保持完整的基质血管部分细胞细胞结构的机械力，其中机械力是切碎和/或均质化。 还公开了使用基质血管部分的方法。