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    Resin-coated metal plate and drawn cans using these plates
    1.
    发明授权
    Resin-coated metal plate and drawn cans using these plates 有权
    树脂涂覆的金属板和使用这些板的拉伸罐

    公开(公告)号：US08133542B2

    公开(公告)日：2012-03-13

    申请号：US12847610

    申请日：2010-07-30

    申请人： Akio Kuroda ,  Takashi Iwai ,  Kazuhiro Sato

    发明人： Akio Kuroda ,  Takashi Iwai ,  Kazuhiro Sato

    IPC分类号： B05D3/02

    CPC分类号： B32B15/08 ,  B32B1/02 ,  B32B7/02 ,  B32B15/09 ,  B32B15/18 ,  B32B15/20 ,  B32B27/08 ,  B32B27/28 ,  B32B27/308 ,  B32B27/36 ,  B32B2255/06 ,  B32B2255/26 ,  B32B2307/554 ,  B32B2307/704 ,  B32B2307/714 ,  B32B2439/66 ,  B32B2439/70 ,  B65D1/28 ,  Y10T428/1355 ,  Y10T428/31681 ,  Y10T428/31786

    摘要： The present invention offers resin-coated metal plates and methods of making, in which the resin-coated metal plates prevent wicket scars from occurring during processes of varnish, print and dry, and there are few scars on the resin-coated surface, the inner surface side of the can and that have superior corrosion resistance and workability, and drawn cans using these metal plates. Therefore, resin film that is applied to the metal plates, is comprised of two layers of a crystallized saturated polyester resin layer [A] and a layer composed of resin [B] that is comprised of saturated polyester resin (i) and ionomer resin (ii), wherein the layer composed of resin [B] is laminated on the above-mentioned metal plate to tightly contact with it, and on the polar surface of the saturated polyester resin layer that is crystallized [A] a highly crystallized layer (X) is formed. A drawn can is formed using the resin-coated metal plate.

    摘要翻译： 本发明提供了树脂涂覆的金属板和制造方法，其中树脂涂覆的金属板防止在清漆，印刷和干燥过程中出现小网痕迹，并且在树脂涂覆表面上几乎没有疤痕 罐的表面侧，具有优异的耐腐蚀性和可加工性，以及使用这些金属板的拉伸罐。 因此，施加到金属板上的树脂膜由两层结晶的饱和聚酯树脂层[A]和由饱和聚酯树脂（i）和离聚物树脂（i）组成的树脂[B]组成的层组成， ii），其中由树脂[B]组成的层层叠在上述金属板上以紧密接触，并且在结晶的饱和聚酯树脂层的极性表面上[A]高度结晶的层（X ） 形成了。 使用树脂涂覆的金属板形成拉伸罐。

    Resin-Coated Metal Plate And Drawn Cans Using These Plates
    2.
    发明申请
    Resin-Coated Metal Plate And Drawn Cans Using These Plates 审中-公开
    树脂涂层金属板和拉丝罐使用这些板

    公开(公告)号：US20070207283A1

    公开(公告)日：2007-09-06

    申请号：US10563008

    申请日：2003-09-08

    申请人： Akio Kuroda ,  Takashi Iwai ,  Kazuhiro Sato

    发明人： Akio Kuroda ,  Takashi Iwai ,  Kazuhiro Sato

    IPC分类号： B32B15/09 ,  B32B1/02

    CPC分类号： B32B15/08 ,  B32B1/02 ,  B32B7/02 ,  B32B15/09 ,  B32B15/18 ,  B32B15/20 ,  B32B27/08 ,  B32B27/28 ,  B32B27/308 ,  B32B27/36 ,  B32B2255/06 ,  B32B2255/26 ,  B32B2307/554 ,  B32B2307/704 ,  B32B2307/714 ,  B32B2439/66 ,  B32B2439/70 ,  B65D1/28 ,  Y10T428/1355 ,  Y10T428/31681 ,  Y10T428/31786

    摘要： The present invention offers resin-coated metal plates that prevent wicket scars from occurring during processes of varnish, print and dry, and there are few scars on the resin-coated surface, the inner surface side of the can and that have superior corrosion resistance and workability, and drawn cans using these metal plates. Therefore, resin film that is applied to the metal plates, is comprised of two layers of a crystallized saturated polyester resin layer [A] and a layer composed of resin [B] that is comprised of saturated polyester resin (i) and ionomer resin (ii), wherein the layer composed of resin [B] is laminated on the above-mentioned metal plate to tightly contact with it, and on the polar surface of the saturated polyester resin layer that is crystallized [A] a highly crystallized layer (X) is formed. The drawn can 10 of the present invention is formed using the resin-coated metal plate 11.

    摘要翻译： 本发明提供了树脂涂覆的金属板，其防止在清漆，印刷和干燥过程中检测到ick痕，并且在树脂涂覆的表面，罐的内表面侧上几乎没有疤痕并具有优异的耐腐蚀性， 可加工性和使用这些金属板的拉伸罐。 因此，施加到金属板上的树脂膜由两层结晶的饱和聚酯树脂层[A]和由饱和聚酯树脂（i）和离聚物树脂（i）组成的树脂[B]组成的层组成， ii），其中由树脂[B]组成的层层叠在上述金属板上以紧密接触，并且在结晶的饱和聚酯树脂层的极性表面上[A]高度结晶的层（X ） 形成了。 使用树脂涂覆金属板11形成本发明的拉伸罐10。

    Resin-Coated Metal Plate And Drawn Cans Using These Plates
    3.
    发明申请
    Resin-Coated Metal Plate And Drawn Cans Using These Plates 有权
    树脂涂层金属板和拉丝罐使用这些板

    公开(公告)号：US20100296898A1

    公开(公告)日：2010-11-25

    申请号：US12847610

    申请日：2010-07-30

    申请人： Akio Kuroda ,  Takashi Iwai ,  Kazuhiro Sato

    发明人： Akio Kuroda ,  Takashi Iwai ,  Kazuhiro Sato

    IPC分类号： B21D51/26 ,  B05D3/02 ,  B05D5/00 ,  B05D1/36

    CPC分类号： B32B15/08 ,  B32B1/02 ,  B32B7/02 ,  B32B15/09 ,  B32B15/18 ,  B32B15/20 ,  B32B27/08 ,  B32B27/28 ,  B32B27/308 ,  B32B27/36 ,  B32B2255/06 ,  B32B2255/26 ,  B32B2307/554 ,  B32B2307/704 ,  B32B2307/714 ,  B32B2439/66 ,  B32B2439/70 ,  B65D1/28 ,  Y10T428/1355 ,  Y10T428/31681 ,  Y10T428/31786

    摘要： The present invention offers resin-coated metal plates and methods of making, in which the resin-coated metal plates prevent wicket scars from occurring during processes of varnish, print and dry, and there are few scars on the resin-coated surface, the inner surface side of the can and that have superior corrosion resistance and workability, and drawn cans using these metal plates. Therefore, resin film that is applied to the metal plates, is comprised of two layers of a crystallized saturated polyester resin layer [A] and a layer composed of resin [B] that is comprised of saturated polyester resin (i) and ionomer resin (ii), wherein the layer composed of resin [B] is laminated on the above-mentioned metal plate to tightly contact with it, and on the polar surface of the saturated polyester resin layer that is crystallized [A] a highly crystallized layer (X) is formed. A drawn can is formed using the resin-coated metal plate.

    摘要翻译： 本发明提供了树脂涂覆的金属板和制造方法，其中树脂涂覆的金属板防止在清漆，印刷和干燥过程中出现小网痕迹，并且在树脂涂覆表面上几乎没有疤痕 罐的表面侧，具有优异的耐腐蚀性和可加工性，以及使用这些金属板的拉伸罐。 因此，施加到金属板的树脂膜由两层结晶的饱和聚酯树脂层[A]和由饱和聚酯树脂（i）和离聚物树脂（i）构成的树脂[B]组成的层 ii），其中由树脂[B]组成的层层叠在上述金属板上以紧密接触，并且在结晶的饱和聚酯树脂层的极性表面上[A]高度结晶的层（X ） 形成了。 使用树脂涂覆的金属板形成拉伸罐。

    METHOD FOR PRODUCING PHOSPHITE DEHYDROGENASE PROTEIN AND USE THEREOF
    4.
    发明申请
    METHOD FOR PRODUCING PHOSPHITE DEHYDROGENASE PROTEIN AND USE THEREOF 有权
    生产磷酸脱氢蛋白的方法及其用途

    公开(公告)号：US20140051134A1

    公开(公告)日：2014-02-20

    申请号：US14113346

    申请日：2012-04-16

    申请人： Akio Kuroda ,  Ryuichi Hirota

    发明人： Akio Kuroda ,  Ryuichi Hirota

    IPC分类号： C12N9/04

    CPC分类号： C12N9/0006 ,  C12N9/0004 ,  C12P17/182 ,  C12Y120/01001

    摘要： In order to provide: a phosphite dehydrogenase protein having both improved solubility and improved heat stability; a method for producing a gene encoding the phosphite dehydrogenase protein; a method for producing the phosphite dehydrogenase protein; and use of the phosphite dehydrogenase protein, (i) a phosphite dehydrogenase protein having a specific amino acid sequence and (ii) a gene encoding the phosphite dehydrogenase protein are used.

    摘要翻译： 为了提供：具有改善的溶解度和改善的热稳定性的亚磷酸酯脱氢酶蛋白质; 生产编码亚磷酸脱氢酶蛋白质的基因的方法; 一种生产亚磷酸脱氢酶蛋白的方法; 和使用亚磷酸酯脱氢酶蛋白质，（i）具有特定氨基酸序列的亚磷酸脱氢酶蛋白质和（ii）编码亚磷酸酯脱氢酶蛋白质的基因。

    Luminescent method for measuring endotoxin
    5.
    发明授权
    Luminescent method for measuring endotoxin 有权
    用于测量内毒素的发光方法

    公开(公告)号：US08394602B2

    公开(公告)日：2013-03-12

    申请号：US12734485

    申请日：2008-11-11

    申请人： Akio Kuroda ,  Kenichi Noda

    发明人： Akio Kuroda ,  Kenichi Noda

    IPC分类号： C12Q1/56

    CPC分类号： G01N21/763 ,  C12Q1/66 ,  G01N33/56911 ,  G01N33/579

    摘要： The present invention provides a method comprising allowing a reaction of a sample, a reagent containing Factor C, which can be activated by binding with endotoxin, and a synthetic luminescent substrate comprising a luminescent substrate bound to a peptide, for release of the luminescent substrate from the synthetic luminescent substrate, allowing a luminescent enzyme to act on the luminescent substrate released in the luminescent substrate release step, for measurement of the luminescence intensity, and quantifying the level of endotoxin in the sample based on a measured value obtained in the luminescence measuring step, the method enabling endotoxin to be simply and quickly measured at a level that cannot be detected in conventional methods for endotoxin measurement, without use of any dedicated measuring device.

    摘要翻译： 本发明提供了一种方法，其包括使样品，可通过与内毒素结合而活化的因子C的试剂与包含与肽结合的发光底物的合成发光底物反应，从而将发光底物从 所述合成发光基板允许发光酶作用于在所述发光底物释放步骤中释放的所述发光基板，用于测量所述发光强度，并且基于在所述发光测量步骤中获得的测量值来定量所述样品中的内毒素水平 ，在不使用任何专用测量装置的情况下，能够以常规的内毒素测量方法无法检测的水平简单且快速地测量内毒素的方法。

    METHOD OF AMPLIFYING ATP AND USER THEREOF
    6.
    发明申请
    METHOD OF AMPLIFYING ATP AND USER THEREOF 有权
    放大ATP及其用途的方法

    公开(公告)号：US20100311093A1

    公开(公告)日：2010-12-09

    申请号：US12769100

    申请日：2010-04-28

    申请人： Akio Kuroda

    发明人： Akio Kuroda

    IPC分类号： C12Q1/48 ,  C12N9/12

    CPC分类号： C12N9/1229 ,  C12N15/62 ,  C12P19/32 ,  C12Q1/485

    摘要： The ATP amplification method is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. Also provided is an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.

    摘要翻译： ATP扩增方法是通过使多磷酸激酶和腺苷酸激酶（不含有ADP的融合蛋白）的融合蛋白（PPK-ADK）作用于不含有ADP的混合物来扩增和检测非常微量的外源ATP的方法 ATP，AMP和多磷酸盐化合物。 还提供了可以扩增和检测单细胞水平的ATP的超灵敏ATP扩增方法，以及基于该ATP扩增方法的超敏感微生物测定法。

    Method of Amplifying Atp and Use Thereof
    7.
    发明申请
    Method of Amplifying Atp and Use Thereof 有权
    放大和使用它的方法

    公开(公告)号：US20080213754A1

    公开(公告)日：2008-09-04

    申请号：US10566957

    申请日：2004-07-27

    申请人： Akio Kuroda

    发明人： Akio Kuroda

    IPC分类号： C12Q1/68 ,  C12P19/30 ,  C12N9/12

    CPC分类号： C12N9/1229 ,  C12N15/62 ,  C12P19/32 ,  C12Q1/485

    摘要： The ATP amplification method of the present invention is a method for amplifying and detecting a very trace amount of exogenous ATP by allowing a fusion protein (PPK-ADK) of a polyphosphate kinase and an adenylate kinase, the fusion protein not containing ADP, to act on a mixture of ATP, AMP, and a polyphosphate compound. The present invention also provides an ultrasensitive ATP amplification method by which ATP at a single cell level can be amplified and detected, and an ultrasensitive microbial assay based on this ATP amplification method.

    摘要翻译： 本发明的ATP扩增方法是通过使多磷酸激酶的融合蛋白（PPK-ADK）和不含ADP的融合蛋白的腺苷酸激酶（PPK-ADK）作用而扩增和检测非常微量的外源ATP的方法 在ATP，AMP和多磷酸盐化合物的混合物上。 本发明还提供了可以扩增和检测单细胞水平的ATP的超灵敏ATP扩增方法，以及基于该ATP扩增方法的超敏感微生物测定法。

    Bias circuit for controlling bias voltage of differential amplifier
    9.
    发明授权
    Bias circuit for controlling bias voltage of differential amplifier 失效
    用于控制差分放大器偏置电压的偏置电路

    公开(公告)号：US5488330A

    公开(公告)日：1996-01-30

    申请号：US361583

    申请日：1994-12-22

    申请人： Hideaki Masuoka ,  Akio Kuroda

    发明人： Hideaki Masuoka ,  Akio Kuroda

    IPC分类号： H03G11/00 ,  H03D7/14 ,  H03F1/32 ,  H03F3/45 ,  H04B1/26

    CPC分类号： H03F3/4521 ,  H03F1/3211 ,  H03F3/45085 ,  H03F3/45089 ,  H03F2203/45366 ,  H03F2203/45542 ,  H03F2203/45544 ,  H03F2203/45574 ,  H03F2203/45702

    摘要： A first differential amplifier includes first and second transistors. A bias circuit includes a second differential amplifier, a constant-current source, and a capacitor. The bases of third and fourth transistors constituting a second differential amplifier, are connected to those of the first and second transistors, respectively. The constant-current source supplies a constant current to the third and fourth transistors, and one end of the capacitor is connected to a connection node of the constant-current source and fourth transistor. In the bias circuit, when a high-level signal is supplied to the base of the first transistor, the capacitor is charged and discharged in accordance with the operations of the third and fourth transistors, with the result that the base potential of the first and second transistors is stabilized, and the operation current flowing through the first differential amplifier is prevented from increasing.

    摘要翻译： 第一差分放大器包括第一和第二晶体管。 偏置电路包括第二差分放大器，恒流源和电容器。 构成第二差分放大器的第三和第四晶体管的基极分别连接到第一和第二晶体管的基极。 恒流源向第三和第四晶体管提供恒定电流，并且电容器的一端连接到恒流源和第四晶体管的连接节点。 在偏置电路中，当向第一晶体管的基极提供高电平信号时，根据第三和第四晶体管的操作对电容器进行充电和放电，结果是第一和第二晶体管的基极电位 第二晶体管稳定，并且防止流过第一差分放大器的工作电流增加。