摘要:
A microarray for predicting the prognosis of neuroblastoma, wherein the microarray has 25 to 45 probes related to good prognosis, which are hybridized to a gene transcript whose expression is increased in a good prognosis patient with neuroblastoma and are selected from 96 polynucleotides consisting of the nucleotide sequences of Seq. ID No. 1 to 96 or their partial continuous sequences or their complementary strands, and 25 to 45 probes related to poor prognosis, which are hybridized to a gene transcript whose expression is increased in a poor prognosis patient with neuroblastoma and are selected from 104 polynucleotides consisting of the nucleotide sequences of Seq. ID No. 97 to 200 or their partial continuous sequences or their complementary strands.
摘要翻译:一种用于预测神经母细胞瘤的预后的微阵列,其中所述微阵列具有与良好预后相关的25至45个探针,其与在具有神经母细胞瘤的良好预后患者中表达增加的基因转录物杂交,并且选自96个由核苷酸 序列Seq。 ID No.1至96或其部分连续序列或其互补链,以及与不良预后相关的25至45个探针,其与在具有神经母细胞瘤的不良预后患者中表达增加的基因转录物杂交并选自104个多核苷酸 由Seq的核苷酸序列组成。 ID号97至200或其部分连续序列或其互补链。
摘要:
A microarray for predicting the prognosis of neuroblastoma, wherein the microarray has 25 to 45 probes related to good prognosis, which are hybridized to a gene transcript whose expression is increased in a good prognosis patient with neuroblastoma and are selected from 96 polynucleotides consisting of the nucleotide sequences of SEQ. ID NOs. 1, 5, 6, 14. 16, 17, 19, 22-24, 28, 29, 31, 37, 39, 40, 43, 44, 47-52, 54, 57-60, 62, 64, 65, 67, 68, 72-75, 77, 78, 80-82, 84, 87, 89-91, 94, 100, 103, 112, 113, 118, 120, 129, 130, 132, 136, 138, 142, 144, 145, 148, 150-153, 155, 158-160, 163-165, 169-171, 173, 174, 177, 178, 180-182, 184, 186, 187, 189, 191, 192, 194, 195, 198-200 or their partial continuous sequences or their complementary strands, and 25 to 45 probes related to poor prognosis, which are hybridized to a gene transcript whose expression is increased in a poor prognosis patient with neuroblastoma and are selected from 104 polynucleotides consisting of the nucleotide sequences of SEQ. ID NOs. 2-4, 7-13, 15, 18, 20, 21, 25-27, 30, 32-36, 38, 41, 42, 45, 46, 53, 55, 56, 61, 63, 66, 69-71, 76, 79, 83, 85, 86, 88, 92, 93, 95-99, 101, 102, 104-111, 114-117, 119, 121-128, 131, 133-135, 137, 139-141, 143, 146, 147, 149, 154, 156, 157, 161, 162, 166-168, 172, 175, 176, 179, 183, 185, 188, 190, 193, 196, 197 or their partial continuous sequences or their complementary strands.
摘要:
In relation to a blade cutting stone or concrete, a blade, preventing wearing of a core while preventing the core from wobbling, cuttable at a stable speed is provided. In a blade formed by providing slots on the outer peripheral edge of a circular core and fixing a superabrasive layer to a portion of the outer peripheral surface of the core located between the slots, the superabrasive layer consists of a first superabrasive layer having an extension formed by partially extending the superabrasive layer toward the inner periphery of the core and a second superabrasive layer. A reinforcing superabrasive layer extending from the outer periphery toward the inner periphery of the core is formed on the inner peripheral side of the second superabrasive layer while the reinforcing superabrasive layer is located closer to the outer periphery than a radial central portion of the core, and an outer peripheral end of the reinforcing superabrasive layer is located closer to the outer periphery than an inner peripheral end of the extension of the first superabrasive layer. The second superabrasive layer can also be provided with an extension having a relatively short radial length with respect to the extension of the first superabrasive layer.
摘要:
The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken β-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
摘要:
The present invention provides methods for producing a minus-strand RNA viral vector, which comprise using a promoter comprising a cytomegalovirus enhancer and a chicken β-actin promoter, to induce the transcription of the genome RNA of a minus-strand RNA viral vector and the expression of minus-strand RNA viral proteins that form a ribonucleoprotein with the genome RNA. The methods of the present invention enable high efficiency production of highly safe minus-strand RNA viral vectors. The methods of the present invention are particularly useful for producing minus-strand RNA viral vectors that are deficient in envelope-constituting protein genes.
摘要:
In relation to a blade cutting stone or concrete, a blade, preventing wearing of a core while preventing the core from wobbling, cuttable at a stable speed is provided. In a blade formed by providing slots on the outer peripheral edge of a circular core and fixing a superabrasive layer to a portion of the outer peripheral surface of the core located between the slots, the superabrasive layer consists of a first superabrasive layer having an extension formed by partially extending the superabrasive layer toward the inner periphery of the core and a second superabrasive layer. A reinforcing superabrasive layer extending from the outer periphery toward the inner periphery of the core is formed on the inner peripheral side of the second superabrasive layer while the reinforcing superabrasive layer is located closer to the outer periphery than a radial central portion of the core, and an outer peripheral end of the reinforcing superabrasive layer is located closer to the outer periphery than an inner peripheral end of the extension of the first superabrasive layer. The second superabrasive layer can also be provided with an extension having a relatively short radial length with respect to the extension of the first superabrasive layer.
摘要:
A POF cable line which comprises a steel pipe and three cable cores drawn in the steel pipe. At least one curved offset section is arranged on the steel pipe at a part spaced from a cable joint at a predetermined distance, with a straight portion of the steel pipe interposed therebetween. The curved offset section prevents the cable cores in the part of the steel pipe from snaking hard and consequently prevents the insulation paper in the part from making soft spots or creases. The snaking occurs easily in the offset section when the cable core thermally expands.
摘要:
F gene-deficient virus virions are successfully recovered by using an F gene-deficient Sendai virus genomic cDNA. Further, F gene-deficient infectious viral particles are successfully constructed by using F-expressing cells as helper cells. Also, F gene and HN gene-deficient virus virions are successfully recovered by using a virus genomic cDNA deficient in both F gene and HN gene. Further, F gene and HN gene-deficient infectious viral particles are successfully produced by using F- and HN-expressing cells as helper cells. A virus deficient in F gene and HN gene and having F protein is constructed by using F-expressing cells as helper cells. In addition, M gene-deficient infectious virus particles were produced using helper cells expressing M protein. From cells infected with M gene-deficient viruses, release of virus-like particles was inhibited. Further, a VSV-G pseudo type virus is successfully constructed by using VSV-G-expressing cells. Techniques for constructing these deficient viruses contribute to the development of vectors of Paramyxoviridae usable in gene therapy.
摘要:
A functional RNP containing negative-strand single-stranded RNA derived from Sendai virus, which has been modified so as not to express at least one envelope protein, has been successfully prepared. An RNP comprising a foreign gene is prepared and inserted into a cell with the use of a cationic liposome, thereby successfully expressing the foreign gene.