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    Method for identifying aptamers
    3.
    发明授权
    Method for identifying aptamers 有权

    公开(公告)号：US09903859B2

    公开(公告)日：2018-02-27

    申请号：US14354597

    申请日：2012-10-25

    申请人： HELMHOLTZ-ZENTRUM FUER UMWELTFORSCHUNG GMBH—UFZ ,  ALBERT-LUDWIGS-UNIVERSITAET FREIBURG

    发明人： Guenter Roth ,  Christine Reinemann ,  Beate Strehlitz

    IPC分类号： C12Q1/68 ,  C07H21/02 ,  C07H21/04 ,  G01N33/53 ,  C12N15/10 ,  C12N5/00

    CPC分类号： G01N33/5308 ,  C12N15/1048 ,  C12Q1/6811 ,  C12Q2525/205

    摘要： The invention relates to a method for identifying aptamers, having the following steps —bringing a mixture of oligonucleotides into contact with an aptamer target structure and binding at least some of the oligonucleotides to the target structure, —separating the oligonucleotides which have been bound to the aptamer target structure from the aptamer target structure and from oligonucleotides that are not bound to the aptamer target structure, —amplifying individual oligonucleotides which were bound to the aptamer target structure in a physically separate manner and producing a plurality of physically separate amplicons, each amplicon predominantly containing one type of oligonucleotides, —specifying a specific marker for a plurality of the physically separate amplicons such that each of the marked amplicons can be uniquely identified using the specified marker of the amplicon, —sequencing oligonucleotides in a plurality of marked amplicons and assigning the marker that is specific for the amplicon to the sequence of the type of oligonucleotides in the amplicon for each amplicon examined by means of the sequencing process —analyzing the binding properties of the types of oligonucleotides to the aptamer target structure and assigning the analyzed binding properties to the specific markers of the amplicons and to the sequences of the types of oligonucleotides.

    BIFUNCTIONAL OLIGONUCLEOTIDE PROBE FOR UNIVERSAL REAL TIME MULTIANALYTE DETECTION
    8.
    发明申请
    BIFUNCTIONAL OLIGONUCLEOTIDE PROBE FOR UNIVERSAL REAL TIME MULTIANALYTE DETECTION 审中-公开
    用于通用实时多媒体检测的双功能寡核苷酸探针

    公开(公告)号：US20140315747A1

    公开(公告)日：2014-10-23

    申请号：US14357574

    申请日：2012-11-12

    申请人： ALBERT-LUDWIGS-UNIVERSITAET FREIBURG

    发明人： Guenter Roth ,  Bernd Faltin ,  Felix Von Stetten ,  Simon Wadle

    IPC分类号： C12Q1/68

    摘要： The invention relates to a mediator probe comprising a probe region and a mediator region. Furthermore, the invention relates to a system comprising a mediator probe and a detection molecule, use of that system and a method for detection of at least one target molecule.

    摘要翻译： 本发明涉及包含探针区域和介体区域的介体探针。 此外，本发明涉及一种包含介体探针和检测分子的系统，该系统的用途和用于检测至少一种靶分子的方法。