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    METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE
    1.
    发明申请
    METHOD OF REDUCING GENE EXPRESSION USING MODIFIED CODON USAGE 审中-公开
    使用改性CODON使用减少基因表达的方法

    公开(公告)号：US20100041107A1

    公开(公告)日：2010-02-18

    申请号：US12446767

    申请日：2007-10-18

    申请人： Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schröder ,  Osker Zelder ,  Weol Kyu Jeong

    发明人： Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schröder ,  Osker Zelder ,  Weol Kyu Jeong

    IPC分类号： C12P13/12 ,  C12P1/00 ,  C12P13/04 ,  C12P13/08 ,  C12P7/64 ,  C12N5/10 ,  C12N5/04 ,  C12N1/21 ,  C12N15/00 ,  C12N15/11

    CPC分类号： C12N15/77

    摘要： The present invention is directed to a method of reducing the amount of at least one polypeptide in a host cell by expressing a nucleotide sequence encoding for the polypeptide in the host cell wherein the nucleotide sequence uses codons that are rarely used according to the codon usage of the host organism. Furthermore, the present invention relates to nucleotide sequences encoding for a polypeptide with a codon usage that has been adjusted to use codons that are only rarely used according to the codon usage of the host organism. The present invention further relates to the use of such sequences and methods for producing fine chemicals such as amino acids, sugars, lipids, oils, carbohydrates, vitamins, cofactors etc.

    摘要翻译： 本发明涉及通过在宿主细胞中表达编码多肽的核苷酸序列来减少宿主细胞中至少一种多肽的量的方法，其中所述核苷酸序列使用根据密码子使用的密码子使用的密码子 宿主生物体。 此外，本发明涉及编码具有密码子使用的多肽的核苷酸序列，其已被调整为使用根据宿主生物的密码子使用很少使用的密码子。 本发明还涉及这样的序列和方法用于生产精细化学品如氨基酸，糖，脂质，油，碳水化合物，维生素，辅因子等的用途。

    Process for the production of cadaverine
    3.
    发明授权
    Process for the production of cadaverine 有权
    生产尸体素的方法

    公开(公告)号：US08741623B2

    公开(公告)日：2014-06-03

    申请号：US12295348

    申请日：2007-03-23

    申请人： Oskar Zelder ,  Weol Kyu Jeong ,  Corinna Klopprogge ,  Andrea Herold ,  Hartwig Schröder

    发明人： Oskar Zelder ,  Weol Kyu Jeong ,  Corinna Klopprogge ,  Andrea Herold ,  Hartwig Schröder

    IPC分类号： C12N1/20 ,  C12N15/74 ,  C12N15/77 ,  A01N63/00 ,  C07H21/04

    CPC分类号： C12P13/02 ,  C12N9/88 ,  C12P13/001

    摘要： Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

    摘要翻译： 通过构建具有失调的赖氨酸脱羧酶基因的重组微生物和选自由天冬氨酸激酶，天冬氨酸二甲醛脱氢酶，二氢吡啶二羧酸合成酶，二氢吡啶二羧酸还原酶，四氢吡啶二羧酸盐琥珀酰基酶，琥珀酰 - 氨基酮基庚酸酯转氨酶，琥珀酰 - 二氨基 - 庚二酸二琥珀酰酶，二氨基庚二酸差向异构酶，二氨基庚二酸脱氢酶，精氨酰-tRNA合成酶，二氨基庚二酸脱羧酶，丙酮酸羧化酶，磷酸烯醇丙酮酸羧化酶，葡萄糖-6-磷酸脱氢酶，转酮酶，转醛醇酶，6-磷酸葡萄糖醛酸酶，果糖1， 6-二磷酸酶，高丝氨酸脱氢酶，磷酸烯醇丙酮酸羧激酶，琥珀酰辅酶A合成酶，甲基丙二酰-CoA变位酶，条件是如果天冬氨酸激酶被释放为基因（i）至少第二个基因（i），除了天冬氨酸激酶外必须解除调节，并培养所述 微生物。

    Process For The Production Of Cadaverine
    5.
    发明申请
    Process For The Production Of Cadaverine 有权
    尸曹的生产过程

    公开(公告)号：US20090246838A1

    公开(公告)日：2009-10-01

    申请号：US12295348

    申请日：2007-03-23

    申请人： Oskar Zelder ,  Weol Kyu Jeong ,  Corinna Klopprogge ,  Andrea Herold ,  Hartwig Schröder

    发明人： Oskar Zelder ,  Weol Kyu Jeong ,  Corinna Klopprogge ,  Andrea Herold ,  Hartwig Schröder

    IPC分类号： C12P13/00 ,  C12N1/00 ,  C12N1/21

    CPC分类号： C12P13/02 ,  C12N9/88 ,  C12P13/001

    摘要： Process for the production of cadaverine by constructing a recombinant microorganism which has a deregulated lysine decarboxylase gene and at least one deregulated gene selected from the group (i) which consists of aspartokinase, aspartatesemialdehyde dehydrogenase, dihydrodipicolinate synthase, dihydrodipicolinate reductase, tetrahydrodipicolinate succinylase, succinyl-amino-ketopimelate transaminase, succinyl-diamino-pimelate desuccinylase, diaminopimelate epimerase, diaminopimelate dehydrogenase, arginyl-tRNA synthetase, diaminopimelate decarboxylase, pyruvate carboxylase, phosphoenolpyruvate carboxylase, glucose-6-phosphate dehydrogenase, transketolase, transaldolase, 6-phosphogluconolactonase, fructose 1,6-biphosphatase, homoserine dehydrogenase, phophoenolpyruvate carboxykinase, succinyl-CoA synthetase, methylmalonyl-CoA mutase, provided that if aspartokinase is deregulated as gene (i) at least a second gene (i) other than aspartokinase has to be deregulated, and cultivating said microorganism.

    摘要翻译： 通过构建具有失调的赖氨酸脱羧酶基因的重组微生物和选自由天冬氨酸激酶，天冬氨酸二甲醛脱氢酶，二氢吡啶二羧酸合成酶，二氢吡啶二羧酸还原酶，四氢吡啶二羧酸盐琥珀酰基酶，琥珀酰 - 氨基酮基庚酸酯转氨酶，琥珀酰 - 二氨基 - 庚二酸二琥珀酰酶，二氨基庚二酸差向异构酶，二氨基庚二酸脱氢酶，精氨酰-tRNA合成酶，二氨基庚二酸脱羧酶，丙酮酸羧化酶，磷酸烯醇丙酮酸羧化酶，葡萄糖-6-磷酸脱氢酶，转酮酶，转醛醇酶，6-磷酸葡萄糖醛酸酶，果糖1， 6-二磷酸酶，高丝氨酸脱氢酶，磷酸烯醇丙酮酸羧激酶，琥珀酰辅酶A合成酶，甲基丙二酰-CoA变位酶，条件是如果天冬氨酸激酶被释放为基因（i）至少第二个基因（i），除了天冬氨酸激酶外必须解除调节，并培养所述 微生物。