公开(公告)号：US20100129785A1

公开(公告)日：2010-05-27

申请号：US12275734

申请日：2008-11-21

申请人： Andrew David Pris ,  Tracy Lynn Paxon ,  Ronald James Wroczynski ,  Anthony John Murray

发明人： Andrew David Pris ,  Tracy Lynn Paxon ,  Ronald James Wroczynski ,  Anthony John Murray

IPC分类号： C12Q1/70 ,  G01N33/543 ,  G01N33/553 ,  G01N33/53 ,  G01N33/569 ,  G01N33/545 ,  G01N33/544

CPC分类号： G01N33/54393

摘要： Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods.

公开(公告)号：US20100129795A1

公开(公告)日：2010-05-27

申请号：US12275753

申请日：2008-11-21

申请人： Andrew David Pris ,  Tracy Lynn Paxon ,  Anthony John Murray

发明人： Andrew David Pris ,  Tracy Lynn Paxon ,  Anthony John Murray

IPC分类号： C12Q1/68 ,  G01N33/566

CPC分类号： G01N33/54306

摘要： Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods.

摘要翻译： 本文公开了用于以单一或多路复用的方式确定样品中靶或多个靶的存在或浓度的试剂，方法和试剂盒。 通常，这些方法可以分析在液相中产生或消耗的小分子，这些分子可以使用气相或气相检测方法进行分析。

公开(公告)号：US20100279272A1

公开(公告)日：2010-11-04

申请号：US12030609

申请日：2008-02-13

申请人： Michael Craig Burrell ,  John Yupeng Gui ,  Frank John Mondello ,  Andrew David Pris ,  Tracy Lynn Paxon

发明人： Michael Craig Burrell ,  John Yupeng Gui ,  Frank John Mondello ,  Andrew David Pris ,  Tracy Lynn Paxon

IPC分类号： C12Q1/70 ,  G01N33/53 ,  G01N33/00

CPC分类号： G01N33/54373 ,  G01N21/658 ,  Y10T436/143333

摘要： Methods are described for performing a multiplexed analysis of a level of target analyte in a sample, employing an identifier and a labeling reagent. Either or both of the identifier and the labeling reagent comprises a SERS-active nanoparticle associated with a SERS-active reporter with a uniquely identifiable spectroscopic signature. Interrogation of the identifier and the labeling reagent is conducted by serial coincident detection. Such methods can provide enhanced multiplexed analysis of analytes in a sample, especially with regards to improving the type of identifying reagents that are employed.

摘要翻译： 描述了使用标识符和标记试剂进行样品中目标分析物水平的多重分析的方法。 标识符和标记试剂中的任一个或两者包含与具有唯一可识别的光谱特征的SERS-活性报道分子相关联的SERS活性纳米颗粒。 标识符和标记试剂的询问通过串行重合检测进行。 这样的方法可以提供样品中分析物的增强的多重分析，特别是关于改进所采用的鉴定试剂的类型。

公开(公告)号：US07518721B2

公开(公告)日：2009-04-14

申请号：US11223353

申请日：2005-09-09

申请人： Michael Craig Burrell ,  Frank J. Mondello ,  Yuan-Hsiang Lee ,  Patrick Ronald Engel ,  Andrew David Pris

发明人： Michael Craig Burrell ,  Frank J. Mondello ,  Yuan-Hsiang Lee ,  Patrick Ronald Engel ,  Andrew David Pris

IPC分类号： G01J3/44

CPC分类号： G01N21/65 ,  G01N21/658

摘要： A lateral flow device is disclosed. The lateral flow device includes a substrate having a flow path and a detection zone disposed along the flow path. The detection zone includes an immobilized target-binding moiety directed against a target of a Raman-active complex. Also disclosed is a method of conducting a lateral flow assay and detection system. The method includes i) defining a flow path having a detection zone; ii) flowing a sample down the flow path; and iii) immobilizing a Raman-active complex if present, at the detection zone. The sample includes a Raman-active complex or a Raman-active tag.

摘要翻译： 公开了一种侧流装置。 横向流动装置包括具有沿着流动路径设置的流路和检测区域的基板。 检测区包括针对拉曼活性复合物的靶的固定的靶结合部分。 还公开了进行侧流测定和检测系统的方法。 该方法包括：i）定义具有检测区域的流路; ii）将样品沿着流动路径流动; 和iii）在检测区域固定拉曼活性复合物（如果存在）。 样品包括拉曼活性复合物或拉曼活性标签。

公开(公告)号：US20100129787A1

公开(公告)日：2010-05-27

申请号：US12275807

申请日：2008-11-21

申请人： Andrew David Pris ,  Frank John Mondello

发明人： Andrew David Pris ,  Frank John Mondello

IPC分类号： C12Q1/70 ,  G01N33/543 ,  G01N33/53 ,  G01N33/569

CPC分类号： G01N33/54306 ,  G01N33/581

摘要： Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods.

公开(公告)号：US07615780B2

公开(公告)日：2009-11-10

申请号：US11427544

申请日：2006-06-29

申请人： Steven Alfred Tysoe ,  Eugene Barash ,  Andrew David Pris

发明人： Steven Alfred Tysoe ,  Eugene Barash ,  Andrew David Pris

IPC分类号： H01L23/58

CPC分类号： H01S5/34333 ,  B82Y20/00 ,  H01S5/005 ,  H01S5/02248 ,  H01S5/18308 ,  H01S5/18338 ,  H01S5/18341

摘要： Disclosed herein are biosensors and methods for making and using the same. In one embodiment, the sensor for detecting an analyte comprises: a substrate, recognition elements specific for the analyte, an excitation source, a detector, a chamber located between the substrate and the excitation source and between the substrate and the detector, and an emission filter. The recognition elements are tethered to the substrate such that the recognition elements can be exposed to a sample. The excitation source is capable of emitting a first light having a first light peak intensity at a first wavelength, wherein the first light can excite a luminophore to emit a second light when the recognition elements interact with the analyte. The detector is capable of detecting the second light emitted by the luminophore. The emission filter is capable of filtering in a band gap that includes the first light peak intensity.

摘要翻译： 本文公开了生物传感器及其制造和使用方法。 在一个实施例中，用于检测分析物的传感器包括：基底，对分析物特异的识别元件，激发源，检测器，位于基底和激发源之间以及基底和检测器之间的腔室，以及发射 过滤。 将识别元件束缚于基底，使得识别元件可以暴露于样品。 激发源能够发射具有第一波长的第一光峰强度的第一光，其中当识别元件与分析物相互作用时，第一光可以激发发光体以发射第二光。 检测器能够检测由发光体发出的第二光。 发射滤波器能够在包括第一光峰强度的带隙中进行滤波。

公开(公告)号：US08318099B2

公开(公告)日：2012-11-27

申请号：US11259711

申请日：2005-10-26

申请人： Radislav Alexandrovich Potyrailo ,  William Guy Morris ,  Kenneth Brakeley Welles, II ,  Andrew Michael Leach ,  Andrew David Pris

发明人： Radislav Alexandrovich Potyrailo ,  William Guy Morris ,  Kenneth Brakeley Welles, II ,  Andrew Michael Leach ,  Andrew David Pris

IPC分类号： G01N27/00

CPC分类号： G06K19/07749 ,  G01N27/3275 ,  G01N27/3278 ,  G06K19/0717 ,  Y10T436/11 ,  Y10T436/25375

摘要： Embodiments of the invention include a wireless sensor, such as an RFID tag, that includes a substrate, an antenna disposed on the substrate, and an environmentally sensitive sensor material disposed over at least a portion of said substrate. Other embodiments an RFID tag and at least one antibody coupled to the RFID tag. The RFID tag includes a substrate, circuitry disposed on the substrate, and an antenna coupled to the substrate. The at least one antibody is capable of affecting the signals emanating from the RFID tag. Further embodiments include a detection system that includes a reader. Yet other embodiments include a method for detecting specific analytes. The method includes providing an RFID tag which emanates a first signal having a first frequency, and enabling the REID tag to emanate a second signal having a second frequency upon attraction of a specific analyte to the RFID tag.

摘要翻译： 本发明的实施例包括诸如RFID标签的无线传感器，其包括基板，设置在基板上的天线以及设置在所述基板的至少一部分上的对环境敏感的传感器材料。 其他实施例是RFID标签和耦合到RFID标签的至少一个抗体。 RFID标签包括衬底，设置在衬底上的电路和耦合到衬底的天线。 所述至少一种抗体能够影响从RFID标签发出的信号。 另外的实施例包括包括读取器的检测系统。 其他实施方案包括用于检测特定分析物的方法。 该方法包括提供发射具有第一频率的第一信号的RFID标签，并且使得REID标签在将特定分析物吸引到RFID标签时能够发出具有第二频率的第二信号。

公开(公告)号：US20090203149A1

公开(公告)日：2009-08-13

申请号：US12030482

申请日：2008-02-13

申请人： Andrew David Pris

发明人： Andrew David Pris

IPC分类号： G01N33/566

CPC分类号： G01N33/54326 ,  G01N33/581

摘要： Processes for improved efficiencies as it relates to the analysis of small molecules whose concentration in the analysis solution is dependent upon the concentration of a target as determined through a liquid phase biological assays with vapor and/or gas phase analysis are disclosed. The process generally includes the competitive or non-competitive binding of target substances onto carrier particles functioning as substrates in the biological assay. Employing the carrier particles as substrates provides increased surface area for the reaction to occur; increased ease of washing steps; and allows for concentration of the increased surface area into a smaller reaction volume prior to introduction into the vapor and/or gas phase spectrometer such as an ion mobility spectrometer.

摘要翻译： 公开了与分析溶液中的浓度取决于通过使用蒸气和/或气相分析的液相生物测定法确定的靶浓度相关的小分子分析的改进效率的方法。 该方法通常包括在生物测定中靶标物质与作为底物起作用的载体颗粒的竞争性或非竞争性结合。 使用载体颗粒作为底物提供增加的反应发生的表面积; 增加洗涤步骤的便利性; 并且允许在引入蒸气和/或气相光谱仪例如离子迁移谱仪之前将增加的表面积浓缩成更小的反应体积。

公开(公告)号：US20090137058A1

公开(公告)日：2009-05-28

申请号：US11946342

申请日：2007-11-28

申请人： Eugene Barash ,  Andrew David Pris

发明人： Eugene Barash ,  Andrew David Pris

IPC分类号： G01N21/64 ,  C12M1/34

CPC分类号： G01N21/645 ,  G01N21/0332 ,  G01N21/55 ,  G01N2021/575 ,  G01N2021/6471

摘要： A device and method for detecting the presence of one or more analytes, bound directly or indirectly to a binding substrate functionalized with a fluorophore, based on measurements of fluorescence and reflectivity. The device and methods comprise an excitation source that emits light capable of being absorbed by a fluorophore and results in the fluorophore's excitation and emission, a fluorescent probe specific for the analyte that is attached via chemisorption to the binding substrate, a detector, and a processor adapted to determine the quantity of the one or more analytes present, by correlating measurements of reflected and fluorescent light.

摘要翻译： 基于荧光和反射率的测量来检测直接或间接结合到用荧光团官能化的结合底物的一种或多种分析物的存在的装置和方法。 该装置和方法包括发射能够被荧光团吸收并导致荧光团的激发和发射的光的激发源，对通过化学吸附附着到结合基底的分析物特异的荧光探针，检测器和处理器 适于通过将反射和荧光的测量相关联来确定存在的一种或多种分析物的量。

公开(公告)号：US20100129786A1

公开(公告)日：2010-05-27

申请号：US12275780

申请日：2008-11-21

申请人： Andrew David Pris ,  Frank John Mondello

发明人： Andrew David Pris ,  Frank John Mondello

IPC分类号： C12Q1/70 ,  G01N33/553 ,  G01N33/569 ,  G01N33/53 ,  C12Q1/28 ,  C12Q1/37 ,  C12Q1/34 ,  C12Q1/42 ,  C12Q1/58

CPC分类号： G01N33/54326 ,  C12Q1/25

摘要： Disclosed herein are agents, methods, and kits for determining the presence or concentration of a target, or multiple targets, in a sample, in a uniplexed or multiplexed fashion. In general, the methods enable the analysis of small molecules produced or consumed in liquid-phase that may be analyzed using gas or vapor phase detection methods.