公开(公告)号：US09175421B2

公开(公告)日：2015-11-03

申请号：US13332411

申请日：2011-12-21

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： C40B30/04 ,  G01N21/552 ,  G01N21/64 ,  G01N33/543 ,  G01N33/557 ,  B01L3/00 ,  G01N21/05 ,  G01N21/21 ,  G01N35/00 ,  G01N21/03

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US08105845B2

公开(公告)日：2012-01-31

申请号：US10578860

申请日：2004-11-14

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： G01N33/543

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method comprises adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. The invention also provides a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点处的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 本发明还提供一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20070087348A1

公开(公告)日：2007-04-19

申请号：US10578860

申请日：2004-11-14

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method comprises adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. The invention also provides a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点处的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 本发明还提供一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US09040309B2

公开(公告)日：2015-05-26

申请号：US11471716

申请日：2006-06-21

申请人： Shai Nimri

发明人： Shai Nimri

IPC分类号： G01N33/548 ,  G01N33/543

CPC分类号： G01N33/54353 ,  G01N33/548

摘要： The invention provides a binding layer comprising a polysaccharide substituted by carboxylic groups or derivatives thereof exhibiting high performance in the binding of ligand molecules and in the interaction thereof with analyte molecules. A method for the preparation of the binding layer and for the assaying of various analyte molecules is also provided.

摘要翻译： 本发明提供一种结合层，其包含被羧基或其衍生物取代的多糖，其在配体分子的结合和与分析物分子的相互作用中表现出高性能。 还提供了制备结合层和分析各种分析物分子的方法。

公开(公告)号：US20070117199A1

公开(公告)日：2007-05-24

申请号：US11471716

申请日：2006-06-21

申请人： Shai Nimri

发明人： Shai Nimri

IPC分类号： C12M1/34

CPC分类号： G01N33/54353 ,  G01N33/548

摘要： The invention provides a binding layer comprising a polysaccharide substituted by carboxylic groups or derivatives thereof exhibiting high performance in the binding of ligand molecules and in the interaction thereof with analyte molecules. A method for the preparation of the binding layer and for the assaying of various analyte molecules is also provided.

摘要翻译： 本发明提供一种结合层，其包含被羧基或其衍生物取代的多糖，其在配体分子的结合和与分析物分子的相互作用中表现出高性能。 还提供了制备结合层和分析各种分析物分子的方法。