公开(公告)号：US09175421B2

公开(公告)日：2015-11-03

申请号：US13332411

申请日：2011-12-21

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： C40B30/04 ,  G01N21/552 ,  G01N21/64 ,  G01N33/543 ,  G01N33/557 ,  B01L3/00 ,  G01N21/05 ,  G01N21/21 ,  G01N35/00 ,  G01N21/03

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20120129723A1

公开(公告)日：2012-05-24

申请号：US13332411

申请日：2011-12-21

申请人： Ariel NOTCOVICH ,  Alon HERSCHHORN ,  Shai NIMRI ,  John BARICH ,  Ariel LIPSON ,  Ran BOAZ ,  Doron LIPSON ,  Yaakov LEVIE

发明人： Ariel NOTCOVICH ,  Alon HERSCHHORN ,  Shai NIMRI ,  John BARICH ,  Ariel LIPSON ,  Ran BOAZ ,  Doron LIPSON ,  Yaakov LEVIE

IPC分类号： C40B30/04 ,  C40B60/08

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： Provided is a method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method includes adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. Also provided is a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 提供了一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 还提供了一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US06970249B1

公开(公告)日：2005-11-29

申请号：US10088654

申请日：2000-09-12

申请人： Stephen Lipson ,  Ariel Notcovich

发明人： Stephen Lipson ,  Ariel Notcovich

IPC分类号： G01N21/55 ,  G01B9/02

CPC分类号： G01N21/553 ,  G01N21/45

摘要： A device for measuring simultaneously the phase at each point of an image formed by light reflected from a sample in which the phase has been modified by plasma resonance in a thin conducting layer, the device comprising a thick transparent substrate (10) on which a thin layer of conducting material is deposited (13); a light source (1); an interferometer; an imaging means (9); and a processing means.

摘要翻译： 一种用于同时测量由在薄导电层中通过等离子体共振修饰相位的样品反射的光形成的每个点处的相位的装置，该器件包括厚的透明衬底（10），薄的透明衬底 沉积导电材料层（13）; 光源（1）; 干涉仪 成像装置（9）; 和处理装置。

公开(公告)号：US08105845B2

公开(公告)日：2012-01-31

申请号：US10578860

申请日：2004-11-14

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ariel Lipson ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： G01N33/543

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method comprises adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. The invention also provides a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点处的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 本发明还提供一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20070087348A1

公开(公告)日：2007-04-19

申请号：US10578860

申请日：2004-11-14

申请人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

发明人： Ariel Notcovich ,  Alon Herschhorn ,  Shai Nimri ,  John Barich ,  Ran Boaz ,  Doron Lipson ,  Yaakov Levie

IPC分类号： C12Q1/68 ,  G01N33/53 ,  G06F19/00

CPC分类号： C40B30/04 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2300/0636 ,  B01L2300/0877 ,  G01N21/05 ,  G01N21/553 ,  G01N21/648 ,  G01N33/54373 ,  G01N33/5438 ,  G01N33/557 ,  G01N2021/0346 ,  G01N2021/212 ,  G01N2021/6439 ,  G01N2021/6463 ,  G01N2035/00158

摘要： A method for determining one or more kinetic parameters of binding between a first binding member and a second binding member. The method comprises adsorbing the first binding member to a surface at a plurality of microspots. The second binding member is then presented to the first binding member at each of the microspots, there being a plurality of combinations of first binding member surface density and second binding member concentration among the plurality of microspots. Data indicative of a binding reaction between the first of microspots are then obtained and analyzed so as to obtain one or more kinetic parameters of the binding between the first and second binding members. The invention also provides a system for carrying out the method. A method for localizing a molecular species at microspots on a surface, and a probe array produced by the method are also provided.

摘要翻译： 一种用于确定第一结合构件和第二结合构件之间的结合的一个或多个动力学参数的方法。 该方法包括将第一结合构件吸附到多个微点处的表面。 然后将第二结合构件提供给每个微点处的第一结合构件，在多个微孔中存在多个第一结合构件表面密度和第二结合成员浓度的组合。 然后获得并分析指示第一个微孔之间的结合反应的数据，以便获得第一和第二结合成员之间的结合的一个或多个动力学参数。 本发明还提供一种用于执行该方法的系统。 还提供了一种用于定位表面上的微孔分子种类的方法，以及通过该方法制备的探针阵列。

公开(公告)号：US20060072113A1

公开(公告)日：2006-04-06

申请号：US10540940

申请日：2002-12-25

申请人： Boaz Ran ,  Ariel Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephan Lipson ,  Doron Lipson

发明人： Boaz Ran ,  Ariel Notcovich ,  Ariel Lipson ,  Shay Nimri ,  Stephan Lipson ,  Doron Lipson

IPC分类号： G01N21/55

CPC分类号： G01N21/55 ,  G01N21/553 ,  G01N2021/058

摘要： An SPR sensor comprising: a thin conducting layer comprising at least one conductive element formed on a surface of a transparent substrate; an illumination system controllable to illuminate an interface between the conducting layer and the substrate; a photosensitive surface that generates signals responsive to light from the light source that is reflected from a region of the interface; a flow cell formed with at least one flow channel having a lumen defined by a wall at least a portion of which is formed from an elastic material and a portion of which is formed by a region of the conducting layer; and at least one hollow needle having an exit orifice communicating with the needle's lumen and wherein fluid flow is enabled between the flow channel and the needle's lumen by puncturing the elastic material with the at least one needle so that the exit orifice communicates with the flow channel lumen.