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39 条记录 (耗时 0.022 秒)

    Compositions and methods for cDNA synthesis
    1.
    发明申请
    Compositions and methods for cDNA synthesis 有权
    cDNA合成的组合物和方法

    公开(公告)号：US20110151520A1

    公开(公告)日：2011-06-23

    申请号：US12632802

    申请日：2009-12-08

    申请人： Ayoub Rashtchian ,  David Schuster

    发明人： Ayoub Rashtchian ,  David Schuster

    IPC分类号： C12P19/34 ,  C12N9/12

    CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/143 ,  C12Q2525/179 ,  C12Q2521/107

    摘要： Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided.Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

    摘要翻译： 提供了制备cDNA分子，通过PCR扩增RNA和制备cDNA文库的方法。 还提供了制备cDNA分子的试剂盒。 还提供组合物，其包括试剂的混合物，包括逆转录酶，缓冲液，辅因子和其它组分，适于立即用于将RNA转化成cDNA和RT PCR，而不进行稀释或添加其它组分。 这些组合物可单独或以试剂盒的形式用于cDNA合成或核酸扩增（例如，通过聚合酶链式反应）或在各种研究，医学，诊断，法医和农业应用中使用逆转录酶的任何方法 。

    Compositions and methods for cDNA synthesis
    3.
    发明授权
    Compositions and methods for cDNA synthesis 有权

    公开(公告)号：US08420324B2

    公开(公告)日：2013-04-16

    申请号：US12632802

    申请日：2009-12-08

    申请人： Ayoub Rashtchian ,  David Schuster

    发明人： Ayoub Rashtchian ,  David Schuster

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/143 ,  C12Q2525/179 ,  C12Q2521/107

    摘要： Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

    Compositions for in vitro amplification of nucleic acids
    4.
    发明申请
    Compositions for in vitro amplification of nucleic acids 审中-公开
    用于体外扩增核酸的组合物

    公开(公告)号：US20050123924A1

    公开(公告)日：2005-06-09

    申请号：US10633629

    申请日：2003-08-05

    申请人： Ayoub Rashtchian ,  David Schuster

    发明人： Ayoub Rashtchian ,  David Schuster

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/686 ,  C12Q2527/125

    摘要： Method and compositions for improving DNA polymerase and reverse transcriptase reactions are provided. Addition of anti-foam reagents to the reactions improves fluid handling, especially of small volumes and allows enhanced accuracy of optical detection, without substantially inhibiting enzymatic activity.

    摘要翻译： 提供了改进DNA聚合酶和逆转录酶反应的方法和组合物。 将反泡沫试剂加入到反应中改善了流体处理，特别是小体积的流动处理，并且允许光学检测的增加的准确性，而基本上不抑制酶活性。

    Compositions and methods for increasing amplification efficiency
    5.
    发明申请
    Compositions and methods for increasing amplification efficiency 审中-公开
    用于增加放大效率的组合物和方法

    公开(公告)号：US20070117114A1

    公开(公告)日：2007-05-24

    申请号：US11481953

    申请日：2006-07-07

    申请人： Ayoub Rashtchian ,  David Schuster

    发明人： Ayoub Rashtchian ,  David Schuster

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/99

    CPC分类号： C12Q1/6848 ,  C12N9/1252 ,  C12N9/99 ,  C12Q1/686 ,  C12Q2549/125 ,  C12Q2549/101 ,  C12Q2521/101 ,  C12Q2527/127 ,  C12Q2521/531 ,  C12Q2521/319

    摘要： Compositions and methods are provided that improve the specificity and efficiency of nucleic acid amplification. A binding partner may be bound to a DNA polymerase enzyme where the binding partner substantially inhibits the activity of the polymerase. A second enzyme modifies the binding partner in a manner that relieves the inhibition of the polymerase activity. The activity of the second enzyme may be inhibited in a temperature-sensitive manner such that the second enzyme is active only at elevated temperatures. As a consequence, the polymerase enzyme also is active only when the temperature is elevated.

    摘要翻译： 提供了提高核酸扩增的特异性和效率的组合物和方法。 结合配偶体可以结合DNA聚合酶，其中结合配偶体基本上抑制聚合酶的活性。 第二种酶以减轻聚合酶活性抑制的方式改变结合配偶体。 可以以温度敏感的方式抑制第二种酶的活性，使得第二种酶仅在升高的温度下是有活性的。 因此，仅当温度升高时，聚合酶也是活性的。

    Compositions and methods for cDNA synthesis
    6.
    发明申请
    Compositions and methods for cDNA synthesis 有权

    公开(公告)号：US20060068436A1

    公开(公告)日：2006-03-30

    申请号：US11237922

    申请日：2005-09-29

    申请人： Ayoub Rashtchian ,  David Schuster

    发明人： Ayoub Rashtchian ,  David Schuster

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12P19/34 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/143 ,  C12Q2525/179 ,  C12Q2521/107

    摘要： Methods for making cDNA molecules, for amplification of RNA by PCR and for preparation of cDNA libraries are provided. Kits for making cDNA molecules also are provided. Compositions are also provided comprising mixtures of reagents, including reverse transcriptases, buffers, cofactors and other components, suitable for immediate use in conversion of RNA into cDNA and RT PCR without dilution or addition of further components. These compositions are useful, alone or in the form of kits, for cDNA synthesis or nucleic acid amplification (e.g., by the Polymerase Chain Reaction) or for any procedure utilizing reverse transcriptases in a variety of research, medical, diagnostic, forensic and agricultural applications.

    Compositions comprising a M-MLV reverse transcriptase and a DNA polymerase and use thereof
    8.
    发明授权
    Compositions comprising a M-MLV reverse transcriptase and a DNA polymerase and use thereof 有权
    包含M-MLV逆转录酶和DNA聚合酶的组合物及其用途

    公开(公告)号：US06495350B1

    公开(公告)日：2002-12-17

    申请号：US09472066

    申请日：1999-12-23

    申请人： Jun E. Lee ,  Ayoub Rashtchian

    发明人： Jun E. Lee ,  Ayoub Rashtchian

    IPC分类号： C12Q168

    CPC分类号： C12P19/34 ,  C12N9/1276 ,  C12N15/1096 ,  C12Q1/6846 ,  C12Q2527/149 ,  C12Q2527/125 ,  C12Q2521/107

    摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulfur-containing molecules or acetate-containing molecules (or combinations of such sulfur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.

    摘要翻译： 本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。 具体地，本发明提供了使用逆转录酶和热稳定DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法 （或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。 因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。 本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。

    Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules
    10.
    发明授权
    Use of deoxyinosine containing primers to balance primer efficiency in the amplification of nucleic acid molecules 失效
    使用含有脱氧蛋白的引物平衡引物在核酸分子扩增中的效率

    公开(公告)号：US5578467A

    公开(公告)日：1996-11-26

    申请号：US246921

    申请日：1994-05-20

    申请人： David M. Schuster ,  Ayoub Rashtchian

    发明人： David M. Schuster ,  Ayoub Rashtchian

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6844 ,  C12Q1/6832 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6862 ,  C12Q2600/156

    摘要： The present invention provides improved methods for amplifying a nucleic acid molecule. Specifically, the invention involves replacing at least one nucleotide of an oligonucleotide with a deoxyinosine residue, so as to more equalize the efficiency with which that oligonucloetide and a second oligonucleotide hybridize to a target molecule, and then amplifying the target molecule using, for example, the polymerase chain reaction. Improved amplification results from the improvement in the relative hybridization efficiencies.

    摘要翻译： 本发明提供了用于扩增核酸分子的改进方法。 具体地说，本发明涉及用脱氧肌苷残基替换寡核苷酸的至少一个核苷酸，从而更均衡该寡核苷酸与第二寡核苷酸与目标分子杂交的效率，然后使用例如， 聚合酶链反应。 改进的扩增来自相对杂交效率的提高。