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1.发明授权Mutant E. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same 有权突变大肠杆菌植酸酶及其天然变体，编码这种植酸酶的核酸，与其结合的载体和宿主细胞以及制备和使用它们的方法公开(公告)号：US07968342B2
公开(公告)日：2011-06-28
申请号：US10524234
申请日：2003-08-11
申请人： Beat O Blattmann , Aldis Darzins , John M. Davis , Lance P. Encell , Thomas B. Morrison , Gregory T. Mrachko , Volker Schellenberger
发明人： Beat O Blattmann , Aldis Darzins , John M. Davis , Lance P. Encell , Thomas B. Morrison , Gregory T. Mrachko , Volker Schellenberger
CPC分类号： C12N15/8243 , C12N9/16 , C12N15/8245
摘要： Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement.
摘要翻译： 提供具有改变活性的产生突变体植酸酶的细胞，以及如此制备的植酸酶。 还提供了制备和生产这种植酸酶的方法以及在饲料中使用表达的植酸酶蛋白作为补充物。
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2.发明申请公开(公告)号：US20080145882A1
公开(公告)日：2008-06-19
申请号：US11978950
申请日：2007-10-30
申请人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
摘要翻译： 本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。
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公开(公告)号：US6133016A
公开(公告)日：2000-10-17
申请号：US851089
申请日：1997-05-05
申请人： Aldis Darzins , Gregory T. Mrachko
发明人： Aldis Darzins , Gregory T. Mrachko
CPC分类号： C12N9/0051 , C10G32/00 , C12N15/52
摘要： The invention relates to a novel microorganism, designated Sphingomonas sp. strain AD109, which is capable of selectively desulfurizing dibenzothiophene. The invention also includes isolated proteins and nucleic acid sequences obtained from this microorganism. In another embodiment, the invention provides a method of using this microorganism or enzyme preparations derived therefrom in the biocatalytic desulfurization of a fossil fuel containing organic sulfur compounds.
摘要翻译： 本发明涉及一种命名为鞘氨醇单胞菌属的新型微生物。 能够选择性地脱硫二苯并噻吩的菌株AD109。 本发明还包括从该微生物获得的分离的蛋白质和核酸序列。 在另一个实施方案中，本发明提供了在含有有机硫化合物的化石燃料的生物催化脱硫中使用该微生物或其衍生的酶制剂的方法。
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4.发明申请公开(公告)号：US20130302880A1
公开(公告)日：2013-11-14
申请号：US13863924
申请日：2013-04-16
申请人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
IPC分类号： C12N9/14
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
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5.发明授权Polynucleotides encoding mutant hydrolase proteins with enhanced kinetics and functional expression 有权编码突变体水解酶蛋白的多核苷酸具有增强的动力学和功能表达公开(公告)号：US08420367B2
公开(公告)日：2013-04-16
申请号：US11978950
申请日：2007-10-30
申请人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
发明人： Aldis Darzins , Lance P. Encell , Rachel Friedman Ohana , Paul Otto , Gediminas Vidugiris , Keith V. Wood , Monika G. Wood , Kris Zimmerman , Michael R. Slater , Kate Qin Zhao
CPC分类号： C12N9/14 , C12N9/96 , C12N11/00 , C12Q1/34 , C12Y308/01 , G01N33/581
摘要： The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins.
摘要翻译： 本发明提供具有增强的动力学和功能表达的突变水解酶蛋白，以及编码突变蛋白的多核苷酸以及使用多核苷酸和突变蛋白的方法。
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6.发明申请Mutant E. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same 有权突变大肠杆菌植酸酶及其天然变体，编码这种植酸酶的核酸，与其结合的载体和宿主细胞以及制备和使用它们的方法公开(公告)号：US20060141562A1
公开(公告)日：2006-06-29
申请号：US10524234
申请日：2003-08-11
申请人： Beat Blattmann , Aldis Darzins , John Davis , Lance Encell , Thomas Morrison , Gregory Mrachko , Volker Schellenberger
发明人： Beat Blattmann , Aldis Darzins , John Davis , Lance Encell , Thomas Morrison , Gregory Mrachko , Volker Schellenberger
CPC分类号： C12N15/8243 , C12N9/16 , C12N15/8245
摘要： Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement.
摘要翻译： 提供具有改变活性的产生突变体植酸酶的细胞，以及如此制备的植酸酶。 还提供了制备和生产这种植酸酶的方法以及在饲料中使用表达的植酸酶蛋白作为补充物。
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7.发明申请Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule 有权通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法公开(公告)号：US20090098627A1
公开(公告)日：2009-04-16
申请号：US12220478
申请日：2008-07-24
申请人： Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Tonny Johnson , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。
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8.发明授权Method of immobilizing a protein or molecule via a mutant dehalogenase that is bound to an immobilized dehalogenase substrate and linked directly or indirectly to the protein or molecule 有权通过与固定化的脱卤酶底物结合并直接或间接连接到蛋白质或分子的突变体脱卤酶固定蛋白质或分子的方法公开(公告)号：US07429472B2
公开(公告)日：2008-09-30
申请号：US11006031
申请日：2004-12-06
申请人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
IPC分类号： C12N9/14 , C12N11/02 , C12N11/14 , C07K14/195
CPC分类号： C12N9/14 , B82Y5/00 , B82Y10/00 , B82Y30/00 , C07K2299/00 , C12N9/0069 , C12Q1/26 , C12Q1/34 , C12Q1/66 , C12Y113/12005 , C12Y308/01005
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。
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公开(公告)号：US08785159B2
公开(公告)日：2014-07-22
申请号：US12950442
申请日：2010-11-19
申请人： Jeffrey G. Linger , Aldis Darzins
发明人： Jeffrey G. Linger , Aldis Darzins
IPC分类号： C12P19/14 , C12N15/00 , C12N5/10 , C12N1/20 , C12N1/00 , C12N1/12 , C12P21/06 , C12P21/04 , C12N9/42
CPC分类号： C07K14/195 , C07K2319/034 , C07K2319/036 , C12N1/22 , C12N9/2437 , C12P19/02 , C12P19/14 , C12Y302/01004
摘要： Nucleic acids encoding secretion signals, expression vectors containing the nucleic acids, and host cells containing the expression vectors are disclosed. Also disclosed are polypeptides that contain the secretion signals and methods of producing polypeptides, including methods of directing the extracellular secretion of the polypeptides. Exemplary embodiments include cellulase proteins fused to secretion signals, methods to produce and isolate these polypeptides, and methods to degrade lignocellulosic biomass.
摘要翻译： 公开了编码分泌信号的核酸，含有核酸的表达载体和含有表达载体的宿主细胞。 还公开了含有分泌信号的多肽和产生多肽的方法，包括引导多肽细胞外分泌的方法。 示例性实施方案包括与分泌信号融合的纤维素酶蛋白，产生和分离这些多肽的方法，以及降解木质纤维素生物质的方法。
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10.发明申请公开(公告)号：US20120220013A1
公开(公告)日：2012-08-30
申请号：US13435970
申请日：2012-03-30
申请人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
发明人： Aldis Darzins , Lance Encell , Dieter Klaubert , Georgyi V. Los , Mark McDougall , Keith V. Wood , Monika G. Wood , Chad Zimprich
CPC分类号： G01N33/581 , C07K7/06 , C07K16/00 , C07K2317/622 , C07K2319/43 , C07K2319/61 , C09B1/00 , C09B11/24 , C09B23/04 , C09B23/06 , C09B23/105 , C12N9/14 , C12Q1/34 , C12Y308/01005 , G01N33/573 , G01N2333/914 , Y10S530/825
摘要： A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.
摘要翻译： 提供任选融合到感兴趣的蛋白质的突变体水解酶。 突变体水解酶能够与相应的非突变（野生型）水解酶的底物形成键，其比野生型水解酶和底物之间形成的键更稳定，并且相对于野生型水解酶具有至少两个氨基酸取代 野生型水解酶。 还提供了包含一个或多个官能团的水解酶的底物，以及使用本发明的突变体水解酶和底物的方法。 还提供能够与底物形成稳定结合的融合蛋白和表达融合蛋白的细胞。
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