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1.发明授权Reagent and method for the detection of a nucleotide sequence with signal amplification 失效用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：US6017707A
公开(公告)日：2000-01-25
申请号：US870730
申请日：1997-06-06
申请人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods include a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
摘要翻译： 检测目的核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分具有 包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。
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2.发明授权Reagent and method for the detection of a nucleotide sequence with signal amplification 失效用于检测具有信号放大的核苷酸序列的试剂和方法
公开(公告)号：US5695936A
公开(公告)日：1997-12-09
申请号：US433505
申请日：1995-06-14
申请人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
发明人： Bernard Mandrand , Philippe Cros , Thierry Delair , Marie-Helene Charles , Marie-Noelle Erout , Christian Pichot
CPC分类号： C12Q1/6813 , C08G81/02 , C12Q1/682
摘要： Methods for the detection of a nucleotide sequence of interest comprising at least one nucleotide probe marked with a tracer. The methods comprise use of a reagent essentially comprising a linear backbone copolymer having lateral substituents, whose chain consists of a first type of repetitive unit and at least one other type of repetitive unit, in which at least one part of the units of the first type have a lateral substituent comprising a nucleotide unit, such a lateral substituent not being present on the other types of units. Each of said nucleotide units, all of which are identical, comprise at least one nucleotide sequence capable of hybridizing with said sequence of interest and nucleotide sequence capable of hybridizing with a probe, the reagent containing on average more than two of said nucleotide units, in molar equivalents, per mole of polymer. Such a reagent enables signal amplification to be obtained, and therefor lowers the sensitivity threshold. Application, in particular, in the production of tests for the detection of pathogenic organisms, or in the diagnosis of genetic diseases.
摘要翻译： PCT No.PCT / FR94 / 01084第 371 1995年6月14日第 102（e）1995年6月14日PCT PCT 1994年9月15日PCT公布。 WO95 / 08000 PCT出版物 1995年3月23日的方法检测目标核苷酸序列的方法包括用示踪剂标记的至少一个核苷酸探针。 所述方法包括使用基本上包含具有侧向取代基的线性主链共聚物的试剂，其链由第一类型的重复单元和至少一种其它类型的重复单元组成，其中第一类型的单元的至少一部分 具有包含核苷酸单元的侧向取代基，这样的侧向取代基不存在于其它类型的单元上。 所述全部相同的所述核苷酸单元包含至少一个能够与所述感兴趣序列杂交的核苷酸序列和能够与探针杂交的核苷酸序列，所述试剂平均包含两个以上的所述核苷酸单元， 摩尔当量，每摩尔聚合物。 这样的试剂能够获得信号放大，从而降低灵敏度阈值。 特别是应用于生产用于检测病原体的检测或遗传疾病的诊断。
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公开(公告)号：US6033853A
公开(公告)日：2000-03-07
申请号：US952397
申请日：1997-01-08
CPC分类号： C12Q1/6834 , C12Q1/682
摘要： Kit for detecting a specific target nucleic acid sequence using a labeled probe with signal amplification, which comprises in containers (i) a probe labeled with a tracer (ii) a suspension of insoluble particles on which are immobilized at least one series of oligonucleotide units all of which are identical and contain at least one sequence that specifically hybridize with the target nucleic acid sequence and at one sequence that specifically hybridize with tracer labeled probe. A method using the kit is also described.
摘要翻译： PCT No.PCT / FR97 / 00483 Sec。 371日期1998年1月8日 102（e）日期1998年1月8日PCT 1997年3月19日PCT公布。 公开号WO97 / 35031 日期1997年9月25日用于使用具有信号扩增的标记探针检测特异性靶核酸序列，其包含在容器中（i）用示踪剂标记的探针（ii）不溶性颗粒的悬浮液，其上固定有至少一种 一系列寡核苷酸单元都是相同的，并且含有至少一个与靶核酸序列特异性杂交的序列，以及与跟踪标记探针特异性杂交的一个序列。 还描述了使用该试剂盒的方法。
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4.发明授权Water-soluble compounds derived from homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological molecules 失效衍生自马来酸酐的均聚物或共聚物的水溶性化合物以及所述化合物用于支持生物分子的应用
公开(公告)号：US5489653A
公开(公告)日：1996-02-06
申请号：US32027
申请日：1993-03-16
IPC分类号： C07K16/18 , C08F8/00 , C08F8/30 , C08F8/34 , G01N33/543 , G01N33/545
CPC分类号： G01N33/545 , C07K16/18 , C08F8/30 , C08F8/34 , G01N33/54313 , G01N33/54353 , Y10S530/815
摘要： Water-soluble compound derived from a homopolymer or copolymer of maleic anhydride having available anhydride functional groups and hydrolyzed anhydride functional groups, wherein the hydrolyzed anhydride functional groups consist of carboxyl functional groups and functional groups derived from carboxyl functional groups carrying a residue of a compound corresponding to the formula I:C.sub.x H.sub.y A.sub.z O.sub.tin which:A is the nitrogen atom of a primary, secondary or tertiary amine functional group or the sulfur atom of a thiol functional group,x, z and t, independently of each other, are non-zero integers, andy is a non-zero integer, not less than 5 when A is a nitrogen atom and not less than 4 when A is a sulfur atom.
摘要翻译： 衍生自具有可用的酸酐官能团的马来酸酐的均聚物或共聚物的水溶性化合物和水解酸酐官能团，其中所述水解酸酐官能团由羧基官能团和衍生自羧基官能团的官能团组成，所述羧基官能团带有相应化合物残基 其中：A是伯，仲或叔胺官能团或硫醇官能团的硫原子的氮原子，x，z和t彼此独立地是非零整数 y为非零整数，A为氮原子时为5以上，A为硫原子时为4以上。
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5.发明授权Water-soluble compounds derived from a homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological molecules 失效衍生自马来酸酐的均聚物或共聚物的水溶性化合物以及所述化合物用于支持生物分子的应用
公开(公告)号：US5439972A
公开(公告)日：1995-08-08
申请号：US321447
申请日：1994-10-12
IPC分类号： C07K16/18 , C08F8/00 , C08F8/30 , C08F8/34 , G01N33/543 , G01N33/545
CPC分类号： G01N33/545 , C07K16/18 , C08F8/30 , C08F8/34 , G01N33/54313 , G01N33/54353 , Y10S530/815
摘要： Water-soluble compounds derived from a homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological moleculesWater-soluble compound derived from a homopolymer or copolymer of maleic anhydride having available anhydride functional groups and hydrolyzed anhydride functional groups, wherein the hydrolyzed anhydride functional groups consist of carboxyl functional groups and functional groups derived from carboxyl functional groups carrying a residue of a compound corresponding to the formula I:C.sub.x H.sub.y A.sub.z O.sub.tin which:- A is the nitrogen atom of a primary, secondary or tertiary amine functional group or the sulfur atom of a thiol functional group,- x, z and t, independently of each other, are non-zero integers, and- y is a non-zero integer, not less than 5 when A is a nitrogen atom and not less than 4 when A is a sulfur atom.
摘要翻译： 衍生自马来酸酐的均聚物或共聚物的水溶性化合物以及所述化合物用于支持生物分子的应用衍生自具有可获得的酸酐官能团的马来酸酐和水解酸酐官能团的均聚物或共聚物的水溶性化合物，其中 水解酸酐官能团由羧基官能团和衍生自羧基官能团的官能团组成，所述羧基官能团带有对应于式I的化合物的残基：C x H y A z O t，其中：A是伯，仲或叔胺官能团的氮原子或 硫醇官能团的硫原子-x，z和t彼此独立地是非零整数，并且-Y是非零整数，当A是氮原子时不小于5 当A为硫原子时，超过4。
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6.发明授权Water-soluble compounds derived from a homopolymer or copolymer of maleic anhydride, and applications of the said compounds to supporting biological molecules 失效衍生自马来酸酐的均聚物或共聚物的水溶性化合物以及所述化合物用于支持生物分子的应用
公开(公告)号：US5760166A
公开(公告)日：1998-06-02
申请号：US597455
申请日：1996-02-02
IPC分类号： C07K16/18 , C08F8/00 , C08F8/30 , C08F8/34 , G01N33/543 , G01N33/545 , C08F8/12
CPC分类号： G01N33/545 , C07K16/18 , C08F8/30 , C08F8/34 , G01N33/54313 , G01N33/54353 , Y10S530/815
摘要： Water-soluble compound derived from a homopolymer or copolymer of maleic anhydride having available anhydride functional groups and hydrolyzed anhydride functional groups, wherein the hydrolyzed anhydride functional groups consist of carboxyl functional groups and functional groups derived from carboxyl functional groups carrying a residue of a compound corresponding to the formula I: C.sub.x H.sub.y A.sub.z O.sub.t in which: A is the nitrogen atom of a primary, secondary or tertiary amine functional group or the sulfur atom of a thiol functional group, x, z and t, independently of each other, are non-zero integers, and y is a non-zero integer, not less than 5 when A is a nitrogen atom and not less than 4 when A is a sulfur atom.
摘要翻译： 衍生自具有可用的酸酐官能团的马来酸酐的均聚物或共聚物的水溶性化合物和水解酸酐官能团，其中所述水解酸酐官能团由羧基官能团和衍生自羧基官能团的官能团组成，所述羧基官能团带有相应化合物残基 其中：A是伯，仲或叔胺官能团或硫醇官能团的硫原子的氮原子，x，z和t彼此独立地是非零整数 y为非零整数，A为氮原子时为5以上，A为硫原子时为4以上。
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7.发明授权Method for isolating proteins or protein and nucleic acid associations, or particle and protein complexes, reagent and uses 有权用于分离蛋白质或蛋白质和核酸关联的方法，或颗粒和蛋白质复合物，试剂和用途公开(公告)号：US07700744B2
公开(公告)日：2010-04-20
申请号：US11320574
申请日：2005-12-30
IPC分类号： A23J1/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。
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公开(公告)号：US07060804B2
公开(公告)日：2006-06-13
申请号：US10182126
申请日：2001-01-22
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The invention concerns a method for isolating proteins and/or protein and nucleic acid associations in a sample, comprising steps which consist in: contacting said sample and magnetic colloidal particles comprising a core and a coat wherein: the core is magnetic and is coated with at least a polymer comprising functional groups X selected among amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups or mixtures thereof, whereof at least one fraction has reacted with other functional groups of the coat, and the coat consists of a polymer bearing functional groups Z and Z′, capable of ionisation, identical or different, selected among amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulphide, α-halogenocarbonyl, sulphonic acid, maleimide, isocyanate and isothiocyanate groups to form a mixture; incubating said mixture in predetermined conditions; and separating from the mixture the protein and/or protein and nucleic acid associations complexed on the colloidal particles by applying a magnetic field. The invention also concerns a complex consisting of colloidal particles and proteins, a reagent comprising such a complex or colloidal particles.
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公开(公告)号：US20100209987A1
公开(公告)日：2010-08-19
申请号：US12656821
申请日：2010-02-17
IPC分类号： C12N13/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
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10.发明授权Method for transporting or storing a protein, a virus, a prokaryotic cell, or an eukaryotic cell 有权运送或储存蛋白质，病毒，原核细胞或真核细胞的方法公开(公告)号：US08093026B2
公开(公告)日：2012-01-10
申请号：US12656821
申请日：2010-02-17
IPC分类号： C12N13/00
CPC分类号： C12N15/1013 , C07K1/22 , G01N33/5434
摘要： The magnetic colloidal particles comprise a core and an envelope in which the core is magnetic and is coated with at least one polymer comprising functional groups X chosen from amine, hydroxyl, thiol, aldehyde, ester, anhydride, acid chloride, carbonate, carbamate, isocyanate and isothiocyanate groups, or mixtures thereof, at least one fraction of which has reacted with other functional groups of the envelope, and the envelope comprises a polymer bearing ionizable functional groups, Z and Z′, which may be identical or different, chosen from amine, carboxylic acid, ester, anhydride, aldehyde, thiol, disulfide, α-halocarbonyl, sulfonic acid, maleimide, isocyanate and isothiocyanate groups, which have partially reacted with the functional groups X of the core. These magnetic colloidal particles can be used to isolate biological material.
摘要翻译： 磁性胶体颗粒包括核心和外壳，其中芯是磁性的并且涂覆有至少一种包含选自胺，羟基，硫醇，醛，酯，酸酐，酰氯，碳酸酯，氨基甲酸酯，异氰酸酯的官能团X的聚合物 和异硫氰酸酯基团或其混合物，其至少一部分已经与外壳的其它官能团反应，并且外壳包含可离子化官能团Z和Z'的聚合物，Z和Z'可以相同或不同，选自胺 ，羧酸，酯，酸酐，醛，硫醇，二硫化物，α-卤代羰基，磺酸，马来酰亚胺，异氰酸酯和异硫氰酸酯基团，其与核的官能团X部分反应。 这些磁性胶体颗粒可用于分离生物材料。
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