公开(公告)号：US20080122428A1

公开(公告)日：2008-05-29

申请号：US10562308

申请日：2004-06-25

申请人： Bernd Kuhn ,  Winfried Denk ,  Gerd Hubener ,  Peter Fromherz

发明人： Bernd Kuhn ,  Winfried Denk ,  Gerd Hubener ,  Peter Fromherz

IPC分类号： G01N21/64 ,  C07D221/18 ,  C07D221/04

CPC分类号： C09B23/12 ,  G01N21/6408

摘要： The present invention relates to a method of determining voltage changes, e.g. in cell membranes, by means of a voltage-sensitive dye.

摘要翻译： 本发明涉及一种确定电压变化的方法，例如， 在细胞膜中，通过电压敏感染料。

公开(公告)号：US06967725B2

公开(公告)日：2005-11-22

申请号：US09971856

申请日：2001-10-05

申请人： Winfried Denk ,  Michale Sean Fee ,  Fritjof Helmchen ,  David William Tank

发明人： Winfried Denk ,  Michale Sean Fee ,  Fritjof Helmchen ,  David William Tank

IPC分类号： G02B21/00 ,  G01B11/24

CPC分类号： G02B21/002

摘要： An optical scanning system includes a probe and a processor. The probe includes a mechanical oscillator responsive to AC voltage signals and an optical fiber. The optical fiber has a free end that executes an oscillatory scanning motion in response to being mechanically driven by the mechanical oscillator. The processor is configured to receive measured intensities of light emitted from spots of a sample scanned by light from the free end of the optical fiber. The processor is also configured to assign intensities to image pixels based on the measured intensities of light. The acts of assigning compensate for variations in the density of the scanned spots.

公开(公告)号：US20050007660A1

公开(公告)日：2005-01-13

申请号：US10493593

申请日：2002-10-25

申请人： Winfried Denk

发明人： Winfried Denk

IPC分类号： G02B21/24 ,  G02B21/00

CPC分类号： G02B21/24

摘要： An imaging device for microscopic imaging of an object using an objective, which is set up for generating an object image in infinity, and a fixed tube optic, which is set up to generate an intermediate image from the object imaged, are described, the objective being situated so it is movable in relation to the tube optic in at least one reference direction, which deviates from the alignment of the optical axis of the objective, and a deflection device having at least one adjustable reflector, which directs the beam path from the objective onto the tube optic in any position of the objective in such a way that it runs perpendicularly to the tube optic and parallel to its optical axis, and an adjustment device are provided, using which the objective and the at least one reflector are movable.

摘要翻译： 描述了一种用于使用用于产生无限远物体图像的物镜的物体的微观成像的成像装置和被设置为从被成像的物体生成中间图像的固定管光学器件，目标 被定位成使得其可以相对于管光学器件在至少一个参考方向上移动，所述至少一个参考方向偏离物镜的光轴的对准，以及具有至少一个可调节反射器的偏转装置，其将光束路径从 将物镜放置在物镜的任何位置上，使得其以垂直于管光学器件并平行于其光轴的方式运行在物镜的任何位置上，并且提供调节装置，使用该调节装置物镜和至少一个反射器是可移动的。

公开(公告)号：US07659993B2

公开(公告)日：2010-02-09

申请号：US11200457

申请日：2005-08-09

申请人： Marcus Feierabend ,  Markus Rückel ,  Winfried Denk

发明人： Marcus Feierabend ,  Markus Rückel ,  Winfried Denk

IPC分类号： G01B11/02

CPC分类号： G01J9/00 ,  G01J9/02 ,  G01N21/6458 ,  G03H1/0443 ,  G03H2001/0458 ,  G03H2001/0467

摘要： A method for sensing a wave-front of specimen light scattered from an illuminated area in a specimen (10) includes the steps of focusing illumination light into the specimen (10), directing specimen light scattered in the specimen (10) to a detector device (50) having a plurality of detector elements (51) and being capable to sense light with local resolution, detecting sample light contained in the specimen light with the detector device (50), said sample light being scattered in a predetermined sample plane (11) of the specimen (10) and being selected by a time-based gating of the specimen light, locally resolved measuring phase information of the sample light, and reconstructing the wave-front of the sample light on the basis of the phase information. Furthermore, a method of microscopic imaging with adapted illumination light is described.

摘要翻译： 用于感测从样本（10）中的照射区域散射的样本光的波前的方法包括将照射光聚焦到样本（10）中的步骤，将在样本（10）中散射的样本光引导到检测器装置 （50）具有多个检测器元件（51）并能够以局部分辨率感测光，利用检测器装置（50）检测样品光中包含的样品光，所述样品光在预定的样品平面（11 ），并且通过对样本光进行基于时间的选通来选择样本光的局部分解测量相位信息，并且基于相位信息重建样本光的波前。 此外，描述了具有适应的照明光的显微成像的方法。

公开(公告)号：US06226112B1

公开(公告)日：2001-05-01

申请号：US09099788

申请日：1998-06-18

申请人： Winfried Denk ,  Gadi Lenz ,  Joseph Shmulovich ,  Chunhui Xu

发明人： Winfried Denk ,  Gadi Lenz ,  Joseph Shmulovich ,  Chunhui Xu

IPC分类号： H04J1408

CPC分类号： H04J14/08

摘要： In a time-division-multiplex system, a relatively high-rate optical signal stream comprising multiple interleaved signal sequences is applied to one end of an elongated waveguide that includes multiple photodetectors disposed along the longitudinal extent of the waveguide. Probe pulses at a relatively low rate are applied to the other end of the waveguide in a synchronized fashion to cause two-photon non-linear absorption in successive respective photodetectors as each propagating probe pulse overlaps successive different signals of each sequence. In that way, electrical output signals are provided from each photodetector at the relatively low probe-pulse rate.

摘要翻译： 在时分多路复用系统中，将包括多个交错信号序列的相对高速率的光信号流施加到细长波导的一端，该波导包括沿波导的纵向延伸设置的多个光电探测器。 以相对低的速率的探针脉冲以同步方式施加到波导的另一端，以在连续的各个光电检测器中引起双光子非线性吸收，因为每个传播的探针脉冲与每个序列的连续的不同信号重叠。 以这种方式，以相对低的探针脉冲速率从每个光电检测器提供电输出信号。

公开(公告)号：US06316950B1

公开(公告)日：2001-11-13

申请号：US08856561

申请日：1997-05-15

申请人： Winfried Denk ,  Chunhui Xu

发明人： Winfried Denk ,  Chunhui Xu

IPC分类号： G01R31265

CPC分类号： H01L22/12 ,  G01R31/311

摘要： Semiconductor devices are imaged using two-photon absorption. The method is similar to conventional optical beam induced imaging except that the light beams used have frequencies (photon energies) insufficient to excite electrons across the semiconductor bandgap. Rather the instantaneous intensity of the lower frequency light is increased, as by using a pulsed laser source, so that electron transitions occur by two-photon absorption predominately in the localized region where the beam is focused. The result is minimal absorption during passage through the substrate and maximal absorption in the component-rich active layer where the beam is focused. This enhances imaging of fine-detail semiconductor devices. Specifically, the quadratic dependence of free carrier generation on the excitation intensity both enhances the resolution and provides a three-dimensional sectioning capability.

摘要翻译： 使用双光子吸收成像半导体器件。 该方法类似于常规光束诱导成像，除了所使用的光束具有不足以激发跨越半导体带隙的电子的频率（光子能量）。 相反地​​，通过使用脉冲激光源，较低频率光的瞬时强度增加，使得电子跃迁主要通过聚焦光束的局部区域中的双光子吸收发生。 其结果是在通过衬底的过程中的最小吸收和在聚焦光束的组分丰富的有源层中的最大吸收。 这增强了精细细节半导体器件的成像。 具体地说，自由载流子产生对激发强度的二次依赖性增强了分辨率并提供了三维切片能力。

公开(公告)号：US5961767A

公开(公告)日：1999-10-05

申请号：US856566

申请日：1997-05-15

申请人： Vladimir A. Aksyuk ,  David J. Bishop ,  Winfried Denk ,  David W. Tank

发明人： Vladimir A. Aksyuk ,  David J. Bishop ,  Winfried Denk ,  David W. Tank

IPC分类号： B05D1/26 ,  B01L3/02 ,  B05C5/00 ,  B05C5/02 ,  B05C9/00 ,  B05C11/00 ,  B05C11/10 ,  B05C11/105 ,  B81C3/00 ,  C09J5/00 ,  C09J163/00 ,  B05D3/02

CPC分类号： B01L3/0262 ,  B05C11/105 ,  B05C5/02 ,  Y10T29/49105 ,  Y10T29/49204 ,  Y10T29/4921 ,  Y10T29/49885

摘要： A method for forming micron-sized or smaller drops of liquid, and the use of the method in fabricating micro electro mechanical and micro mechanical devices is disclosed. A micropipette is formed having an inside diameter no larger than the size of the drops to be formed. The micropipette is connected to a system capable of developing a positive and optionally negative pressure within the micropipette. The tip of the micropipette is placed in liquid. The liquid is drawn into the micropipette via capillary action or from the negative pressure developed by the system. The micropipette is then positioned to deliver liquid to an intended location on a surface. To deliver the liquid, a positive pressure is developed within the micropipette. The positive pressure forces a micron-sized or smaller drop of liquid out of the micropipette. The method can be used to form micron-sized or smaller drops of adhesive for fixing in place various structural members that form microdevices.

摘要翻译： 公开了用于形成微米级或更小液滴的方法，以及在制造微机电和微机械装置中使用该方法。 形成具有不大于要形成的液滴的尺寸的内径的微量移液管。 微量移液管连接到能够在微量移液管内形成正面和可选的负压的系统。 微量吸头的尖端放置在液体中。 液体通过毛细作用或由系统产生的负压吸入微量吸液管。 然后将微量移液器定位成将液体输送到表面上的预期位置。 为了输送液体，在微量移液管内产生正压力。 正压迫使微米尺寸或更小的液滴从微量移液管中移出。 该方法可以用于形成用于固定各种形成微型装置的结构构件的微米级或更小的粘合剂滴。

公开(公告)号：US4684206A

公开(公告)日：1987-08-04

申请号：US563715

申请日：1983-12-20

申请人： Johannes G. Bednorz ,  Winfried Denk ,  Martin Lanz ,  Wolfgang D. Pohl

发明人： Johannes G. Bednorz ,  Winfried Denk ,  Martin Lanz ,  Wolfgang D. Pohl

IPC分类号： G02B6/00 ,  G01Q80/00 ,  G01Q90/00 ,  G02B6/02 ,  G02B27/00 ,  G11B7/08 ,  G11B7/135 ,  G11B7/22 ,  H01S5/10 ,  G02B6/10 ,  B23P15/00 ,  B29D11/00

CPC分类号： G02B6/02 ,  G11B7/08 ,  G11B7/1387 ,  H01S5/10 ,  G01Q80/00

摘要： This light waveguide consists of an optically transparent body cut at one end to a sharp tip and polished optically flat at the other end. A metallization layer on its surface is thick enough to be opaque. By pressing the waveguide against a rigid plate the metallization is plastically deformed so as to expose a tiny aperture at the tip of the body through which light can pass. By carefully controlling the deformation of the metallization the diameter of the aperture can be kept between 10 and 500 nm. The waveguide can be incorporated in a semiconductor laser of a read/write head used in an optical storage device.

摘要翻译： 该光波导由在一端切割成尖锐的光学透明体并在另一端光学平坦地抛光。 其表面上的金属化层足够厚以使其不透明。 通过将波导压靠在刚性板上，金属化被塑性变形，以便暴露出能够通过光的身体的尖端处的微小孔。 通过仔细地控制金属化的变形，孔的直径可以保持在10和500nm之间。 波导可以结合在光存储器件中使用的读/写头的半导体激光器中。

公开(公告)号：US09799098B2

公开(公告)日：2017-10-24

申请号：US12597406

申请日：2008-04-24

申请人： H. Sebastian Seung ,  Joseph F. Murray ,  Viren Jain ,  Srinivas C. Turaga ,  Moritz Helmstaedter ,  Winfried Denk

发明人： H. Sebastian Seung ,  Joseph F. Murray ,  Viren Jain ,  Srinivas C. Turaga ,  Moritz Helmstaedter ,  Winfried Denk

IPC分类号： G06K9/40 ,  G06T5/00 ,  G06K9/34 ,  G06K9/46 ,  G06T7/11

CPC分类号： G06T5/001 ,  G06K9/342 ,  G06K9/4628 ,  G06T7/11 ,  G06T2207/20084

摘要： Identifying objects in images is a difficult problem, particularly in cases an original image is noisy or has areas narrow in color or grayscale gradient. A technique employing a convolutional network has been identified to identify objects in such images in an automated and rapid manner. One example embodiment trains a convolutional network including multiple layers of filters. The filters are trained by learning and are arranged in successive layers and produce images having at least a same resolution as an original image. The filters are trained as a function of the original image or a desired image labeling; the image labels of objects identified in the original image are reported and may be used for segmentation. The technique can be applied to images of neural circuitry or electron microscopy, for example. The same technique can also be applied to correction of photographs or videos.

公开(公告)号：US20100183217A1

公开(公告)日：2010-07-22

申请号：US12597406

申请日：2008-04-24

申请人： H. Sebastian Seung ,  Joseph F. Murray ,  Viren Jain ,  Srinivas C. Turaga ,  Moritz Helmstaedter ,  Winfried Denk

发明人： H. Sebastian Seung ,  Joseph F. Murray ,  Viren Jain ,  Srinivas C. Turaga ,  Moritz Helmstaedter ,  Winfried Denk

IPC分类号： G06K9/62

CPC分类号： G06T5/001 ,  G06K9/342 ,  G06K9/4628 ,  G06T7/11 ,  G06T2207/20084

摘要： Identifying objects in images is a difficult problem, particularly in cases an original image is noisy or has areas narrow in color or grayscale gradient. A technique employing a convolutional network has been identified to identify objects in such images in an automated and rapid manner. One example embodiment trains a convolutional network including multiple layers of filters. The filters are trained by learning and are arranged in successive layers and produce images having at least a same resolution as an original image. The filters are trained as a function of the original image or a desired image labeling; the image labels of objects identified in the original image are reported and may be used for segmentation. The technique can be applied to images of neural circuitry or electron microscopy, for example. The same technique can also be applied to correction of photographs or videos.

摘要翻译： 识别图像中的对象是一个困难的问题，特别是在原始图像嘈杂或具有颜色或灰度梯度窄的区域的情况下。 已经鉴定了采用卷积网络的技术，以自动和快速的方式识别这些图像中的对象。 一个示例性实施例训练包括多层滤波器的卷积网络。 过滤器通过学习进行训练，并且以连续的层布置并产生具有与原始图像至少相同分辨率的图像。 根据原始图像或期望的图像标记来对滤波器进行训练; 报告原始图像中识别的对象的图像标签，并可用于分割。 该技术可以应用于例如神经电路或电子显微镜的图像。 相同的技术也可以应用于照片或视频的校正。