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    Butynol I esterase
    2.
    发明授权
    Butynol I esterase 有权
    丁醇I酯酶

    公开(公告)号：US08008051B2

    公开(公告)日：2011-08-30

    申请号：US12422785

    申请日：2009-04-13

    申请人： Bernhard Hauer ,  Thomas Friedrich ,  Christoph Nübling ,  Rainer Stürmer ,  Wolfgang Ladner

    发明人： Bernhard Hauer ,  Thomas Friedrich ,  Christoph Nübling ,  Rainer Stürmer ,  Wolfgang Ladner

    IPC分类号： C12P7/64 ,  C12P7/62 ,  C12N9/18

    CPC分类号： C12N9/16 ,  C12P41/00

    摘要： The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefore, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

    摘要翻译： 本发明涉及具有酯酶活性，特别是丁酸酯酶活性的来自假单胞菌的新型蛋白质，涉及编码的核酸序列，表达盒，载体和重组微生物; 涉及用于制备所述蛋白质的方法及其用于酶，特别是对映选择性酶，酯水解或酯交换有机酯的用途。

    Butynol I esterase
    3.
    发明申请
    Butynol I esterase 有权
    丁醇I酯酶

    公开(公告)号：US20100196970A1

    公开(公告)日：2010-08-05

    申请号：US12422785

    申请日：2009-04-13

    申请人： Bernhard Hauer ,  Thomas Friedrich ,  Christoph Nübling ,  Rainer Stürmer ,  Wolfgang Ladner

    发明人： Bernhard Hauer ,  Thomas Friedrich ,  Christoph Nübling ,  Rainer Stürmer ,  Wolfgang Ladner

    IPC分类号： C12P7/00

    CPC分类号： C12N9/16 ,  C12P41/00

    摘要： The invention relates to novel proteins from Pseudomonas glumae, having esterase activity, in particular butynol I esterase activity, to nucleic acid sequences coding therefor, to expression cassettes, vectors and recombinant microorganisms; to methods for preparing said proteins and to the use thereof for enzymic, in particular enantioselective enzymic, ester hydrolysis or transesterification of organic esters.

    摘要翻译： 本发明涉及具有酯酶活性，特别是丁酸酯酶活性的编码的核酸序列，表达盒，载体和重组微生物的新型蛋白质， 涉及用于制备所述蛋白质的方法及其用于酶，特别是对映选择性酶，酯水解或酯交换有机酯的用途。

    METHOD FOR THE ENZYMATIC REDUCTION OF ALPHA- AND BETA-DEHYDROAMINO ACIDS USING ENOATE REDUCTASES
    6.
    发明申请
    METHOD FOR THE ENZYMATIC REDUCTION OF ALPHA- AND BETA-DEHYDROAMINO ACIDS USING ENOATE REDUCTASES 审中-公开
    使用降低氧化还原酶抑制阿尔法和β-去氢氨基酸的方法

    公开(公告)号：US20100304448A1

    公开(公告)日：2010-12-02

    申请号：US12746973

    申请日：2008-12-08

    申请人： Rainer Stürmer ,  Bernhard Hauer ,  Thomas Friedrich ,  Kurt Faber ,  Melanie Hall ,  Clemens Stückler

    发明人： Rainer Stürmer ,  Bernhard Hauer ,  Thomas Friedrich ,  Kurt Faber ,  Melanie Hall ,  Clemens Stückler

    IPC分类号： C12P13/04

    CPC分类号： C12P13/04

    摘要： A method for the enzymatic preparation of amino acids of the general formula (3) or (4) from alpha-dehydroamino acids of the general formula (1) or (2) wherein R1, R2 are independently of one another H, C1-C6 alkyl, C2-C6 alkenyl, an optionally substituted carbo- or heterocyclic, aromatic or nonaromatic radical, or an alkylaryl radical, or a carboxyl radical (—COOR), R3 is H, formyl, acetyl, propionyl, benzyl, benzyloxycarbonyl, BOC, Alloc, R is H, C1-C6 alkyl, aryl, by reducing a compound of the formula (1) or (2) in the presence of a reductase.

    摘要翻译： 由通式（1）或（2）的α-脱氢氨基酸酶制备通式（3）或（4）的氨基酸的方法，其中R 1，R 2彼此独立地为H，C 1 -C 6 烷基，C 2 -C 6烯基，任选取代的碳 - 或杂环芳族或非芳族基团，或烷基芳基或羧基（-COOR），R 3为H，甲酰基，乙酰基，丙酰基，苄基，苄氧基羰基， 通过在还原酶的存在下还原式（1）或（2）的化合物，R分子是H，C 1 -C 6烷基，芳基。

    Enzymatic Reduction for Producing Optically Active Alcohols
    7.
    发明申请
    Enzymatic Reduction for Producing Optically Active Alcohols 有权
    用于生产光活性醇的酶减量

    公开(公告)号：US20080318288A1

    公开(公告)日：2008-12-25

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Kesseler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。

    Enzymatic reduction for producing optically active alcohols
    9.
    发明授权
    Enzymatic reduction for producing optically active alcohols 有权
    用于制备光学活性醇的酶还原

    公开(公告)号：US07888080B2

    公开(公告)日：2011-02-15

    申请号：US12158390

    申请日：2006-12-13

    申请人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    发明人： Rainer Stürmer ,  Maria Keβeler ,  Bernhard Hauer ,  Thomas Friedrich ,  Michael Breuer ,  Hartwig Schröder

    IPC分类号： C12P17/00 ,  C12N9/14

    CPC分类号： C07D333/16 ,  C07D333/20 ,  C12P7/02 ,  C12P11/00 ,  C12P41/002

    摘要： A process for preparing optically active alkanols of the formula I in which n is an integer from 0 to 5; Cyc is an optionally substituted, mono- or polynuclear, saturated or unsaturated, carbocyclic or heterocyclic ring, and R1 is halogen, SH, OH, NO2, NR2R3 or NR2R3R4+X−, with R2, R3 and R4 independently of one another being hydrogen or a lower alkyl or lower alkoxy radical and X− being a counterion, which process comprises incubating in a medium comprising alkanone of the formula II  in which n, Cyc and R1 are as defined above, an enzyme having a polypeptide sequence (i) SEQ ID NO: 1 or (ii) in which, compared to SEQ ID NO:1, up to 25% of the amino acid radicals have been altered by deletion, insertion, substitution or a combination thereof and which retains at least 50% of the enzymic activity of SEQ ID NO:1, with the compound of the formula II being enzymically reduced to give the compound of the formula I, and isolating the product formed.

    摘要翻译： 一种制备式I的光学活性链烷醇的方法，其中n为0至5的整数; Cyc是任选取代的单或多核饱和或不饱和的碳环或杂环，R1是卤素，SH，OH，NO2，NR2R3或NR2R3R4 + X-，R2，R3和R4彼此独立地是氢 或低级烷基或低级烷氧基，X是抗衡离子，该方法包括在包含式II的烷酮的培养基中孵育，其中n，Cyc和R 1如上定义，具有多肽序列（i）SEQ ID NO： SEQ ID NO：1或（ii）其中与SEQ ID NO：1相比，通过缺失，插入，取代或其组合改变多达25％的氨基酸基团，并且其保留至少50％的 酶活性为SEQ ID NO：1，其中式II化合物被酶还原，得到式I化合物，并分离形成的产物。