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    Product quality enhancement in mammalian cell culture processes for protein production
    1.
    发明申请
    Product quality enhancement in mammalian cell culture processes for protein production 有权
    用于蛋白质生产的哺乳动物细胞培养过程中的产品质量增强

    公开(公告)号：US20050084933A1

    公开(公告)日：2005-04-21

    申请号：US10740645

    申请日：2003-12-18

    申请人： Bernhard Schilling ,  Scott Gangloff ,  Dharti Kothari ,  Kirk Leister ,  Linda Matlock ,  Stephen Zegarelli ,  Christoph Joosten ,  Jonathan Basch ,  Sivakesava Sakhamuri ,  Steven S. Lee

    发明人： Bernhard Schilling ,  Scott Gangloff ,  Dharti Kothari ,  Kirk Leister ,  Linda Matlock ,  Stephen Zegarelli ,  Christoph Joosten ,  Jonathan Basch ,  Sivakesava Sakhamuri ,  Steven S. Lee

    IPC分类号： C07K14/705 ,  C12P21/00 ,  C12P21/02 ,  C12N5/06

    CPC分类号： C07K14/70521 ,  C12N2500/34 ,  C12P21/005

    摘要： The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, illustratively, but not limited to, fed-batch cell cultures. The methods comprise feeding the cells with D-galactose, preferably with feed medium containing D-galactose, preferably daily, to sustain a sialylation effective level of D-galactose in the culture for its duration, thus increasing sialylation of the produced proteins. The methods can also comprise at least two temperature shifts performed during the culturing period, in which the temperature is lower at the end of the culturing period than at the time of initial cell culture. The cell culture processes of the invention involving two or more temperature shifts sustain a high cell viability, and can allow for an extended protein production phase. The methods can also comprise the delayed addition of polyanionic compound at a time after innoculation. Supplementation of the cultures with D-galactose, preferably in a feed medium, to sustain galactose at sialylation effective levels in the cultures until the end of a culture run reverses a decline in sialylation that accompanies culture scale up, and is advantageous for large scale culturing processes.

    摘要翻译： 本发明描述了通过动物细胞或哺乳动物细胞培养物生产蛋白质，特别是糖蛋白的方法和方法，例如但不限于补料分批细胞培养物。 所述方法包括用D-半乳糖喂养细胞，优选用含有D-半乳糖的饲料培养基，优选每天喂养，以维持培养基中唾液酸化有效水平的D-半乳糖的持续时间，从而增加产生的蛋白质的唾液酸化。 所述方法还可以包括在培养期间进行的至少两个温度漂移，其中在培养期结束时温度比初始细胞培养时温度更低。 涉及两个或多个温度变化的本发明的细胞培养方法维持高的细胞活力，并且可以允许延长的蛋白质生产阶段。 所述方法还可以包括在接种后延迟加入聚阴离子化合物。 用D-半乳糖，优选在饲料培养基中补充培养物，以维持培养物中唾液酸化有效水平的半乳糖，直至培养结束时反转伴随培养物扩大的唾液酸化的下降，并且有利于大规模培养 过程。

    Mammalian ceII culture processes for protein production
    3.
    发明申请
    Mammalian ceII culture processes for protein production 有权
    用于蛋白质生产的哺乳动物ceII培养方法

    公开(公告)号：US20050019859A1

    公开(公告)日：2005-01-27

    申请号：US10742564

    申请日：2003-12-18

    申请人： Bernhard Schilling ,  Linda Matlock ,  Stephen Zegarelli ,  William Burnett ,  Christoph Joosten ,  Jonathan Basch ,  Sivakesava Sakhamuri ,  Steven Lee

    发明人： Bernhard Schilling ,  Linda Matlock ,  Stephen Zegarelli ,  William Burnett ,  Christoph Joosten ,  Jonathan Basch ,  Sivakesava Sakhamuri ,  Steven Lee

    IPC分类号： C07K14/705 ,  C12N5/00 ,  C12N5/02 ,  C12P21/00 ,  C07K14/74

    CPC分类号： C12P21/005 ,  C07K14/70521 ,  C12N5/0018 ,  C12N2500/34 ,  C12N2500/74 ,  C12N2501/33 ,  C12N2501/90 ,  C12N2501/905 ,  C12N2501/91 ,  C12N2510/02

    摘要： The present invention describes methods and processes for the production of proteins, particularly glycoproteins, by animal cell or mammalian cell culture, preferably, but not limited to, fed-batch cell cultures. In one aspect, the methods comprise at least two temperature shifts performed during the culturing period, in which the temperature is lower at the end of the culturing period than at the time of initial cell culture. Throughout their duration, the culturing processes of the invention involving two or more downward shifts in temperature sustain a high viability of the cultured cells, and can yield an increased end titer of protein product, and a high quality of protein product, as determined, e.g., by sialic acid content of the produced protein. In another aspect, the methods comprise the delayed addition of polyanionic compound during the culturing period. The delayed addition of polyanionic compound sustains a high viability of the cultured cells, and can extend the growth phase, delay the onset of the death phase, and arrest the death phase.

    摘要翻译： 本发明描述了通过动物细胞或哺乳动物细胞培养物生产蛋白质，特别是糖蛋白的方法和方法，优选但不限于补料分批细胞培养物。 在一个方面，所述方法包括在培养期间进行的至少两个温度漂移，其中在培养期结束时温度比初始细胞培养时的温度更低。 在整个持续时间内，涉及两个或更多个温度向下移位的本发明的培养方法维持培养细胞的高活力，并且可以产生蛋白质产物的最终滴定度和蛋白质产物的高质量，如所确定的，例如 ，通过产生蛋白质的唾液酸含量。 另一方面，所述方法包括在培养期间延迟加入聚阴离子化合物。 聚阴离子化合物的延迟添加维持培养细胞的高活力，并且可以延长生长期，延缓死亡阶段的发作并停止死亡期。

    Determining an encoding type of data
    4.
    发明授权
    Determining an encoding type of data 有权
    确定数据的编码类型

    公开(公告)号：US08175844B2

    公开(公告)日：2012-05-08

    申请号：US12494840

    申请日：2009-06-30

    申请人： Mori Nobuyoshi ,  Bernhard Schilling ,  Martin Schmidt

    发明人： Mori Nobuyoshi ,  Bernhard Schilling ,  Martin Schmidt

    IPC分类号： G06F17/18 ,  G06F17/30

    CPC分类号： G06F17/10

    摘要： Implementations of the present disclosure provide for determining an encoding type of data. Implementations include receiving a data set from a computer-readable storage medium, decoding the data set using a first encoding type to provide a first plurality of numbers, generating a first distribution based on the first plurality of numbers, decoding the data set using a second encoding type to provide a second plurality of numbers, and generating a second distribution based on the second plurality of numbers. An actual encoding type of the data set is determined based on the first distribution, the second distribution and an expected distribution, and the data set is processed based on the actual encoding type.

    摘要翻译： 本公开的实现提供了确定数据的编码类型。 实现包括从计算机可读存储介质接收数据集，使用第一编码类型对数据集进行解码以提供第一多个数字，基于第一多个数字生成第一分布，使用第二编码解码数据集 编码类型以提供第二多个数字，以及基于所述第二多个数字生成第二分布。 基于第一分布，第二分布和预期分布来确定数据集的实际编码类型，并且基于实际编码类型处理数据集。

    DETERMINING AN ENCODING TYPE OF DATA
    5.
    发明申请
    DETERMINING AN ENCODING TYPE OF DATA 有权
    确定编码类型的数据

    公开(公告)号：US20100332184A1

    公开(公告)日：2010-12-30

    申请号：US12494840

    申请日：2009-06-30

    申请人： Mori Nobuyoshi ,  Bernhard Schilling ,  Martin Schmidt

    发明人： Mori Nobuyoshi ,  Bernhard Schilling ,  Martin Schmidt

    IPC分类号： G06F17/18 ,  G06F17/30 ,  G06F7/556

    CPC分类号： G06F17/10

    摘要： Implementations of the present disclosure provide for determining an encoding type of data. Implementations include receiving a data set from a computer-readable storage medium, decoding the data set using a first encoding type to provide a first plurality of numbers, generating a first distribution based on the first plurality of numbers, decoding the data set using a second encoding type to provide a second plurality of numbers, and generating a second distribution based on the second plurality of numbers. An actual encoding type of the data set is determined based on the first distribution, the second distribution and an expected distribution, and the data set is processed based on the actual encoding type.

    摘要翻译： 本公开的实现提供了确定数据的编码类型。 实现包括从计算机可读存储介质接收数据集，使用第一编码类型对数据集进行解码以提供第一多个数字，基于第一多个数字生成第一分布，使用第二编码解码数据集 编码类型以提供第二多个数字，以及基于所述第二多个数字生成第二分布。 基于第一分布，第二分布和预期分布来确定数据集的实际编码类型，并且基于实际编码类型处理数据集。

    Time zone identifier
    7.
    发明申请
    Time zone identifier 审中-公开
    时区标识符

    公开(公告)号：US20070121425A1

    公开(公告)日：2007-05-31

    申请号：US11291245

    申请日：2005-11-30

    申请人： Markus Eble ,  Gundula Niemann ,  Bernhard Schilling

    发明人： Markus Eble ,  Gundula Niemann ,  Bernhard Schilling

    IPC分类号： G04B19/22

    CPC分类号： G04G9/0076

    摘要： A time zone identifier for a geographic location is generated by receiving a request, including a first information portion in the time zone identifier that represents a time difference between a standard time and a local base time for the geographic location, and, if there exists a rule specifying when the geographic location observes daylight saving time, including a second information portion identifying the rule in the time zone identifier. Otherwise, the second information portion is not included. A computer-readable data structure for a time zone identifier includes a first attribute for the first information portion and a second attribute for the second information portion. A computer system includes a rule repository and a time zone management module.

    摘要翻译： 通过接收包括时区标识符中的第一信息部分的表示生成地理位置的时区标识符，该第一信息部分表示地理位置的标准时间和本地基准时间之间的时间差，并且如果存在 指定地理位置何时观察夏令时的规则，包括标识时区标识符中的规则的第二信息部分。 否则，不包括第二信息部分。 用于时区标识符的计算机可读数据结构包括第一信息部分的第一属性和第二信息部分的第二属性。 计算机系统包括规则库和时区管理模块。

    Setting gauge for setting a depth stop on a handpiece for medical purposes
    8.
    发明授权
    Setting gauge for setting a depth stop on a handpiece for medical purposes 失效
    设置用于医疗用途的手持件上设置深度限位的量规

    公开(公告)号：US06499221B1

    公开(公告)日：2002-12-31

    申请号：US09527591

    申请日：2000-03-17

    申请人： Bernhard Kuhn ,  Bernhard Schilling

    发明人： Bernhard Kuhn ,  Bernhard Schilling

    IPC分类号： A61C300

    CPC分类号： B23B49/005 ,  A61C5/44 ,  B23B2260/088

    摘要： The invention relates to a setting gauge (21) for setting a depth stop (6) on a handpiece (1), which has a connection device (3) for the releasable connection of a pin-shaped tool (4) with the handpiece (1), in particular for setting a depth stop (6) on a handpiece (1) for a working of a root canal (W2) in a tooth (Z). So that the setting of the depth stop can be effected more simply and with little manual effort, the setting gauge (21) has a connection element for connecting and positioning the setting gauge (21) on the handpiece (1) and at least one scale (27a, 27b, 27c) on the setting gauge body (22) which, in the position of the setting gauge (21) connected with the handpiece (1), extends parallel to the depth stop (6) and its setting path.

    摘要翻译： 本发明涉及一种用于在手持件（1）上设置深度止挡件（6）的设定规（21），其具有用于将销状工具（4）与手持件可释放地连接的连接装置（3） 特别是用于在用于牙齿（Z）中的根管（W2）的加工的手持件（1）上设置深度止挡件（6）。 因此，深度限位器的设置可以更简单且更少地进行人工操作，设定规（21）具有连接元件，用于连接和定位手持件（1）上的设定规（21）和至少一个刻度 （21）的与手持件（1）连接的位置与设置计量器主体（22）平行的深度限制（6）及其设定路径延伸的设置量筒体（27a，27b，27c）。

    Medical and/or dental instrument with oscillatory rod
    10.
    发明授权
    Medical and/or dental instrument with oscillatory rod 有权

    公开(公告)号：US06616450B2

    公开(公告)日：2003-09-09

    申请号：US09924302

    申请日：2001-08-08

    申请人： Walter Mössle ,  Bernhard Schilling ,  Gerd Löhn

    发明人： Walter Mössle ,  Bernhard Schilling ,  Gerd Löhn

    IPC分类号： A61C108

    CPC分类号： A61C17/20

    摘要： The invention relates to a medical or dental-medical treatment instrument (1) having a grip sleeve (11), an oscillatory rod (13) which extends longitudinally in the grip sleeve (11) and is mounted therein in a radially or also axially elastically yielding manner, and an oscillation generator (31) for transmitting oscillations to the oscillatory rod (13). In order to attain a compact construction, the oscillatory rod (13) is of a base part and an attachment part which is fixed on the base part.