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    XYLOSE ISOMERASE AND XYLITOL DEHYDROGENASE COMBINATION FOR XYLOSE FERMENTATION TO ETHANOL AND B. fragilis XYLOSE ISOMERASE
    6.
    发明申请
    XYLOSE ISOMERASE AND XYLITOL DEHYDROGENASE COMBINATION FOR XYLOSE FERMENTATION TO ETHANOL AND B. fragilis XYLOSE ISOMERASE 审中-公开
    XYLOSE异黄酮和XYLITOL DEHYDROGENASE组合，用于XYLOSE发酵至乙醇和B.脆弱物XYLOSE异构体

    公开(公告)号：US20140017768A1

    公开(公告)日：2014-01-16

    申请号：US13996305

    申请日：2011-12-09

    申请人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

    发明人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

    IPC分类号： C12N15/81

    CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/92 ,  C12P7/10 ,  C12Y101/01009 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

    摘要： Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. When grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of axylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

    摘要翻译： 本文公开了一种新发现的问题和解决方案，用于工程酿酒酵母发酵木糖以制备使用木糖异构酶的乙醇以将木糖转化为木酮糖，以通过木酮糖激酶进入戊糖磷酸途径。 当在含有木糖木糖醇的培养基上生长时，尽管在酿酒酵母中没有木糖还原酶活性，但是在细胞中趋于积聚。 木糖醇抑制木糖异构酶的活性。 所描述的一种溶液是同时表达外源性木糖异构酶外源木糖醇脱氢酶，而在不存在木糖还原酶表达的情况下，任选也可以过表达木酮糖激酶。 另一种溶液是来自脆弱拟杆菌的木糖异构酶，木糖异构酶比其它木糖异构酶抑制较少，木糖异构酶例如是大肠杆菌木糖异构酶。 脆弱拟杆菌木糖异构酶的表达可以单独使用，或与木糖醇脱氢酶的表达组合使用，任选地过度表达木酮糖激酶以改善从木糖产生乙醇。

    Xylose isomerase and xylitol dehydrogenase combination for xylose fermentation to ethanol and B. fragilis xylose isomerase
    8.
    发明授权
    Xylose isomerase and xylitol dehydrogenase combination for xylose fermentation to ethanol and B. fragilis xylose isomerase 有权
    木糖异构酶和木糖醇脱氢酶组合用于木糖发酵成乙醇和脆弱木糖木糖异构酶

    公开(公告)号：US09365857B2

    公开(公告)日：2016-06-14

    申请号：US13996305

    申请日：2011-12-09

    申请人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

    发明人： Sean Jordan ,  Beth Fatland-Bloom ,  Lhing-Yew Li

    IPC分类号： C12P7/06 ,  C12P7/10 ,  C12N9/90 ,  C12N9/04 ,  C12N1/19 ,  C12N15/81 ,  C12N9/92

    CPC分类号： C12N15/81 ,  C12N9/0006 ,  C12N9/92 ,  C12P7/10 ,  C12Y101/01009 ,  C12Y503/01005 ,  Y02E50/16 ,  Y02E50/17 ,  Y02P20/52

    摘要： Disclosed herein is a newly discovered problem and solution for engineering S. cerevisiae to ferment xylose to make ethanol utilizing xylose isomerase to convert xylose to xylulose for entry, via xylulokinase, into the pentose phosphate pathway. The problem is that when grown on a media containing xylose xylitol tends to accumulate in the cell despite the absence of xylose reductase activity in S. cerevisiae. Xylitol inhibits the activity of xylose isomerases. One solution described is to simultaneously express an exogenous xylitol dehydrogenase along with the exogenous xylose isomerase while optionally also overexpressing xylulokinase in the absence of expression of a xylose reductase. Another solution is a xylose isomerase from Bacteroides fragilis which is less inhibited by xylitol than other xylose isomerases, exemplified by E. coli xylose isomerase. Expression of the Bacteroides fragilis xylose isomerase may be used alone, or in combination with expression of a xylitol dehydrogenase and optionally over expression of xylulokinase to improve ethanol production from xylose.

    摘要翻译： 本文公开了一种新发现的问题和解决方案，用于工程酿酒酵母发酵木糖以制备乙醇，利用木糖异构酶将木糖转化成木酮糖，通过木酮糖激酶进入戊糖磷酸途径。 问题是，当在含有木糖木糖醇的培养基上生长时，尽管在酿酒酵母中不存在木糖还原酶，但仍倾向于在细胞中积累。 木糖醇抑制木糖异构酶的活性。 所描述的一种溶液是同时表达外源性木糖异构酶外源木糖醇脱氢酶，而在不存在木糖还原酶表达的情况下，任选也可以过表达木酮糖激酶。 另一种溶液是来自脆弱拟杆菌的木糖异构酶，木糖异构酶比其它木糖异构酶抑制较少，木糖异构酶例如是大肠杆菌木糖异构酶。 脆弱拟杆菌木糖异构酶的表达可以单独使用，或与木糖醇脱氢酶的表达和任选地过度表达木酮糖激酶一起使用以改善从木糖产生乙醇。