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    Nucleotide sequence encoding the dapC gene and process for the production of L-lysine
    1.
    发明申请
    Nucleotide sequence encoding the dapC gene and process for the production of L-lysine 有权
    编码dapC基因的核苷酸序列和生产L-赖氨酸的方法

    公开(公告)号：US20050100927A1

    公开(公告)日：2005-05-12

    申请号：US10745684

    申请日：2003-12-29

    申请人： Bettina Mockel ,  Anke Weissenborn ,  Walter Pfefferle ,  Michael Hartmann ,  Jorn Kalinowski ,  Alfred Puhler

    发明人： Bettina Mockel ,  Anke Weissenborn ,  Walter Pfefferle ,  Michael Hartmann ,  Jorn Kalinowski ,  Alfred Puhler

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N15/31 ,  C12N15/54 ,  C12P13/08 ,  C12Q1/68 ,  C12R1/13

    CPC分类号： C12P13/08 ,  C12N9/1096

    摘要： The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.

    摘要翻译： 本发明涉及来自棒状细菌的分离的多核苷酸，其含有至少一种多核苷酸序列，所述多核苷酸序列选自a）多核苷酸，其与编码含有根据SEQ ID No.3所述的氨基酸序列的多肽的多核苷酸至少70％相同。 2，b）编码多肽的多核苷酸，其含有与SEQ ID NO：1的氨基酸序列至少70％相同的氨基酸序列。 2）c）与a）或b）的多核苷酸互补的多核苷酸，和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及d）发酵生产的方法 的L-氨基酸，特别是L-赖氨酸。

    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE ZWA1 GENE
    3.
    发明申请
    NUCLEOTIDE SEQUENCES WHICH CODE FOR THE ZWA1 GENE 失效
    编号为ZWA1基因的核苷酸序列

    公开(公告)号：US20060205046A1

    公开(公告)日：2006-09-14

    申请号：US11419529

    申请日：2006-05-22

    申请人： Bettina Mockel ,  Walter Pfefferle ,  Achim Marx ,  Jorn Kalinowski ,  Brigitte Bathe ,  Alfred Puhler

    发明人： Bettina Mockel ,  Walter Pfefferle ,  Achim Marx ,  Jorn Kalinowski ,  Brigitte Bathe ,  Alfred Puhler

    IPC分类号： C12P13/08 ,  C12N15/74 ,  C12N1/21

    CPC分类号： C07K14/34 ,  C12N9/00 ,  C12P13/08

    摘要： Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.

    摘要翻译： 本文公开了一种分离的多核苷酸，其包含选自以下的多核苷酸序列：a）与编码包含SEQ ID NO：2的氨基酸序列的多肽的多核苷酸的程度相同的多核苷酸 b）编码多肽的多核苷酸，其包含与SEQ ID NO：2的氨基酸序列至少70％相同程度的氨基酸序列c）与a）和 b）和d）包含a），b）或c）的多核苷酸序列的至少15个连续核苷酸的多核苷酸，以及在所用棒状细菌中扩增zwa1基因的L-氨基酸的发酵制剂的方法。

    Plasmids from Corynebacterium glutamicum and use thereof
    9.
    发明授权
    Plasmids from Corynebacterium glutamicum and use thereof 有权
    谷氨酸棒杆菌的质粒及其用途

    公开(公告)号：US06777229B1

    公开(公告)日：2004-08-17

    申请号：US09704725

    申请日：2000-11-03

    申请人： Andreas Tauch ,  Jorn Kalinowski ,  Alfred Puhler ,  Georg Thierbach

    发明人： Andreas Tauch ,  Jorn Kalinowski ,  Alfred Puhler ,  Georg Thierbach

    IPC分类号： C12N1563

    CPC分类号： C12N15/77 ,  C12P13/02 ,  C12P13/08

    摘要： This invention relates to the mutually compatible plasmids pTET3 and pCRY4, isolated from the strain of Corynebacterium glutamicum deposited under DSM number 5616, wherein plasmid pTET3 is characterised by 1.1 a length of ˜27.8 kbp and the restriction map shown in FIG. 1, 1.2 a replication region comprising the nucleotide sequence shown in SEQ ID no. 1 and 1.3 an antibiotic resistance region consisting of a tetA gene imparting tetracycline resistance and an aadA gene imparting streptomycin and spectinomycin resistance, shown in SEQ ID no. 6, and plasmid pCRY4 is characterised by 1.4 a length of ˜48 kbp and the restriction map shown in FIG. 2 and 1.5 a replication region comprising the nucleotide sequence shown in SEQ ID no. 4 to composite plasmid vectors of these plasmids which are capable of autonomous replication in coryneform bacteria and to processes for the production of L-amino acids, vitamins and nucleotides using these bacteria.

    摘要翻译： 本发明涉及从DSM号5616中沉积的谷氨酸棒杆菌菌株分离的相互相容的质粒pTET3和pCRY4，其中质粒pTET3的特征在于1.1，长度为约27.8kbp，图1所示的限制性图谱。 1,1.2复制区，其包含SEQ ID No： 1和1.3由SEQ ID No.3所示的赋予四环素抗性的tetA基因和赋予链霉素和壮观霉素抗性的aadA基因组成的抗生素抗性区域。 6，并且质粒pCRY4的特征在于1.4〜48kbp的长度以及图4所示的限制性图谱。 2和1.5复制区，其包含SEQ ID No： 这些质粒能够在棒状细菌中自主复制的这些质粒的复合质粒载体和使用这些细菌生产L-氨基酸，维生素和核苷酸的方法。

    Mutant E. coli kiz strains for production of pantothenic acid
    10.
    发明授权
    Mutant E. coli kiz strains for production of pantothenic acid 有权
    用于生产泛酸的突变大肠杆菌kiz菌株

    公开(公告)号：US06171845B2

    公开(公告)日：2001-01-09

    申请号：US09416756

    申请日：1999-10-12

    申请人： Frank Elischweski ,  Jorn Kalinowski ,  Alfred Puhler ,  Nicole Dusch ,  Jurgen Dohmen ,  Mike Farwick ,  Georg Thierbach

    发明人： Frank Elischweski ,  Jorn Kalinowski ,  Alfred Puhler ,  Nicole Dusch ,  Jurgen Dohmen ,  Mike Farwick ,  Georg Thierbach

    IPC分类号： C12N121

    CPC分类号： C12N9/0008 ,  C12P7/42

    摘要： The invention relates to a process for the preparation and improvement of D-pantothenic acid-producing microorganisms by amplification of nucleotide sequences which code for ketopantoate reductase, in particular the panE gene, individually or in combination with one another, and optionally additionally of the ilvC gene, the microorganisms containing these nucleotide sequences, and a process for the preparation of D-pantothenic acid comprising fermentation of these microorganisms, concentration of pantothenic acid in the medium or in the cells of the microorganisms, and isolation of the D-pantothenic acid.

    摘要翻译： 本发明涉及通过扩增编码酮戊酸还原酶，特别是panE基因的核苷酸序列来单独地或彼此组合并任选地另外添加ilvC来制备和改进D-泛酸产生微生物的方法 基因，含有这些核苷酸序列的微生物，以及D-泛酸的制备方法，其包括这些微生物的发酵，在培养基中或微生物细胞中浓缩泛酸，以及分离D-泛酸。